Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A16 F258 I0 R1 432 103.0 5191697 92.7% 4812703 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,214,964 A→G 100% I229V (ATT→GTT)  metA → homoserine O‑transsuccinylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,214,9640AG94.2% 375.8 / 8.4 121I229V (ATT→GTT) metAhomoserine O‑transsuccinylase
Reads supporting (aligned to +/- strand):  ref base A (6/1);  new base G (57/57);  total (63/58)
Fisher's exact test for biased strand distribution p-value = 1.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.65e-01

Only 100 of 123 total aligned reads displayed.

CTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGACCCGGATGTACCGT  >  NC_000913/4214867‑4215060
                                                                                                 |                                                                                                
cTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCAtt                                                                                                >  2:1550125/1‑100 (MQ=255)
 tttCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTg                                                                                               <  2:2161702/100‑1 (MQ=255)
 tttCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTg                                                                                               <  1:49420/100‑1 (MQ=255)
  ttCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGc                                                                                              <  2:1845803/100‑1 (MQ=255)
  ttCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGc                                                                                              <  1:1643098/100‑1 (MQ=255)
   tCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGcc                                                                                             >  1:1845954/1‑100 (MQ=255)
    ccGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCt                                                                                            <  1:2345576/100‑1 (MQ=255)
     cGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCtt                                                                                           >  2:2123664/1‑100 (MQ=255)
     cGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCtt                                                                                           >  1:2117081/1‑100 (MQ=255)
       gCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAATGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTtg                                                                                         <  1:1330066/100‑1 (MQ=255)
       gCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTtg                                                                                         <  2:1861534/100‑1 (MQ=255)
        cAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCttt                                                                                          <  1:580523/98‑1 (MQ=255)
        cAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTtgt                                                                                        <  2:38737/100‑1 (MQ=255)
        cAGCGTTGATTCGTGATTACACCGACCTGGAAATTCTGGCAGAGACCGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTtgt                                                                                        >  1:1404665/1‑100 (MQ=255)
         aGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTtgtn                                                                                       <  1:1338794/100‑2 (MQ=255)
         aGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTtgtg                                                                                       <  1:18561/100‑1 (MQ=255)
            gTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACg                                                                                    <  2:145310/100‑1 (MQ=255)
             ttGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACgg                                                                                   >  2:1163485/1‑100 (MQ=255)
              tGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACggg                                                                                  <  2:2276260/100‑1 (MQ=255)
              tGATTCGTGATTACACCGATCTCGAAATTCTAGCAGATACGGAAGAATGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACggg                                                                                  <  1:1749946/100‑1 (MQ=255)
               gATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGc                                                                                 <  1:2451240/100‑1 (MQ=255)
                   cGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATc                                                                             >  1:2285341/1‑100 (MQ=255)
                     tGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCCGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATccc                                                                           <  1:1594397/100‑1 (MQ=255)
                     tGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATccc                                                                           <  1:741618/100‑1 (MQ=255)
                         tACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAat                                                                       <  1:1189671/100‑1 (MQ=255)
                         tACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAat                                                                       <  1:1033583/100‑1 (MQ=255)
                          acacCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAata                                                                      <  2:133688/100‑1 (MQ=255)
                          acacCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAata                                                                      <  2:2080138/100‑1 (MQ=255)
                          acacCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAat                                                                       >  2:2077408/1‑99 (MQ=255)
                          acacCGAGCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAata                                                                      <  2:102630/100‑1 (MQ=255)
                           cacCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAatat                                                                     >  2:1993901/1‑100 (MQ=255)
                           cacCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAatat                                                                     >  1:397428/1‑100 (MQ=255)
                             ccGATCTGGCAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAACGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATatga                                                                   >  1:2170735/1‑100 (MQ=255)
                             ccGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATatga                                                                   >  2:1170738/1‑100 (MQ=255)
                             ccGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATatga                                                                   <  1:930327/100‑1 (MQ=255)
                             ccGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATatga                                                                   <  2:785839/100‑1 (MQ=255)
                             ccGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATatga                                                                   >  1:144509/1‑100 (MQ=255)
                             ccGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATatga                                                                   >  2:2398553/1‑100 (MQ=255)
                               gATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATatgatg                                                                 <  2:2431843/100‑1 (MQ=255)
                                  cTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATgcgc                                                              >  2:337307/1‑100 (MQ=255)
                                   tGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCa                                                             >  2:830225/1‑100 (MQ=255)
                                   tGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCa                                                             <  1:412456/100‑1 (MQ=255)
                                      aaaTTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAAc                                                          <  1:2389016/100‑1 (MQ=255)
                                         ttCTGGCAGAGACGGAAGAAGGGGATGCATCTCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGTCCATCCCGAATGTGATGCGGaaggggt                                                       >  1:800198/1‑95 (MQ=255)
                                           cTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTgg                                                     <  1:48674/100‑1 (MQ=255)
                                           cTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTg                                                      >  1:1857120/1‑99 (MQ=255)
                                           cTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGAAGGGCCATCCCGGATATGATGCGCATACg                                                         >  1:2495305/1‑96 (MQ=255)
                                             ggCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGgcg                                                   >  1:1341652/1‑100 (MQ=255)
                                             ggCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGgcg                                                   >  2:617248/1‑100 (MQ=255)
                                              gCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGgcgc                                                  >  2:1817764/1‑100 (MQ=255)
                                              gCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGgcgc                                                  <  1:2561653/100‑1 (MQ=255)
                                               cAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCa                                                 <  2:1250040/100‑1 (MQ=255)
                                                agagACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAg                                                <  2:115828/100‑1 (MQ=255)
                                                  agaCGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGa                                              >  1:1027171/1‑100 (MQ=255)
                                                   gaCGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGaa                                             >  2:1073670/1‑100 (MQ=255)
                                                        aagaagGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATACCGAATATGATGCGCAAACGCTGGCGCAGGAAttttt                                        >  1:479730/1‑100 (MQ=255)
                                                          gaagGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTcc                                      <  2:493410/100‑1 (MQ=255)
                                                           aagGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCcg                                     <  2:674038/100‑1 (MQ=255)
                                                            agGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCcgc                                    <  2:144510/100‑1 (MQ=255)
                                                             ggggATGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCcgcg                                   <  2:1246572/100‑1 (MQ=255)
                                                                 aTGCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGAtgt                               <  2:153288/100‑1 (MQ=255)
                                                                   gCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATCTGATGCGCAACCGCTGGCGCAGGACTTTTTCCGCGATGTgg                             >  1:1189344/1‑100 (MQ=255)
                                                                   gCATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGAtgtg                              >  1:2345789/1‑99 (MQ=255)
                                                                    cATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGa                            >  2:268003/1‑100 (MQ=255)
                                                                    cATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGa                            >  1:414249/1‑100 (MQ=255)
                                                                    cATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGa                            >  1:57366/1‑100 (MQ=255)
                                                                    cATATCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGa                            >  1:1284023/1‑100 (MQ=255)
                                                                     atatCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGaa                           <  1:202113/100‑1 (MQ=255)
                                                                      tatCTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAg                          >  1:565346/1‑100 (MQ=255)
                                                                         cTGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCg                       >  2:1782400/1‑100 (MQ=255)
                                                                          tGTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCgg                      <  1:441186/100‑1 (MQ=255)
                                                                           gTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCgta                     >  1:2006261/1‑98 (MQ=255)
                                                                           gTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGa                     >  1:2145205/1‑100 (MQ=255)
                                                                           gTTTGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGa                     >  1:1768560/1‑100 (MQ=255)
                                                                              tggcAGGAAAGATAAGCGCGTTGGCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGGTGGCGCAGGAATTTTTCCGAGATGTGGAAGCCGGACt                   <  1:2442212/96‑1 (MQ=255)
                                                                              tGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGGGATGTGGAAGCCGGGCTa                  >  1:401089/1‑100 (MQ=255)
                                                                              tGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTa                  <  1:829354/100‑1 (MQ=255)
                                                                              tGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTa                  >  1:1515797/1‑100 (MQ=255)
                                                                              tGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTa                  <  2:2199106/100‑1 (MQ=255)
                                                                              tGCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTa                  >  1:1017776/1‑100 (MQ=255)
                                                                              tGCCAGCAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATATTTCCGCGATGTGGAAGCCGGACTa                  >  2:198540/1‑100 (MQ=255)
                                                                               gCCAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAg                 <  1:1785357/100‑1 (MQ=255)
                                                                                ccAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGa                >  2:1139182/1‑100 (MQ=255)
                                                                                 cAGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGAc               >  1:613570/1‑100 (MQ=255)
                                                                                  aGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGAcc              >  1:531830/1‑100 (MQ=255)
                                                                                  aGTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGAcc              >  2:2387665/1‑100 (MQ=255)
                                                                                   gTAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGAccc             <  2:2379466/100‑1 (MQ=255)
                                                                                    tAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGACCCg            <  2:1381519/100‑1 (MQ=255)
                                                                                    tAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGACCCg            >  1:2175578/1‑100 (MQ=255)
                                                                                    tAAAGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGACCCg            >  2:1931481/1‑100 (MQ=255)
                                                                                    tAAAGATAAGCGCGTTGCCTGTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGACCCg            <  2:1387178/100‑1 (MQ=255)
                                                                                     aaaGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGACCCgg           >  2:308009/1‑100 (MQ=255)
                                                                                      aaGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGACCCGGa          <  2:1027174/100‑1 (MQ=255)
                                                                                      aaGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGACCCGGa          <  1:733929/100‑1 (MQ=255)
                                                                                       aGATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGACCCGGAt         <  1:664601/100‑1 (MQ=255)
                                                                                        gATAAGCGCGTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGACCCGGATg        >  2:237065/1‑100 (MQ=255)
                                                                                        gATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGACCCGGATg        >  1:21405/1‑100 (MQ=255)
                                                                                            aGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGACCCGGATGTAcc    >  2:849460/1‑100 (MQ=255)
                                                                                              cgcgTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGACCCGGATGTACCGt  <  2:621670/100‑1 (MQ=255)
                                                                                              cgcgTTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGAAATTTTTCCGCGATGTGGAAGCCGGACTAGACCCGGATGTACCGt  <  2:621818/100‑1 (MQ=255)
                                                                                                 |                                                                                                
CTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGACCCGGATGTACCGT  >  NC_000913/4214867‑4215060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A