Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F244 I0 R1 270 114.4 5840133 93.3% 5448844 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,008,075 A→G 100% D189G (GAC→GGC)  ygeX → 2,3‑diaminopropionate ammonia lyase, PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,008,0750AG98.1% 347.0 / ‑2.8 103D189G (GAC→GGC) ygeX2,3‑diaminopropionate ammonia lyase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (54/47);  total (55/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.40e-01

Only 100 of 105 total aligned reads displayed.

TCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGAAATGG  >  NC_000913/3007979‑3008170
                                                                                                |                                                                                               
tCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGgcac                                                                                              >  2:524414/1‑100 (MQ=255)
tCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGgcac                                                                                              >  1:168867/1‑100 (MQ=255)
  ggTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAACACGGCTGGGAAGTGGTACAGGGCACgg                                                                                            >  1:1955984/1‑100 (MQ=255)
   gTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGc                                                                                           >  1:491042/1‑100 (MQ=255)
     gCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCAt                                                                                         >  1:1268549/1‑100 (MQ=255)
      ccGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATg                                                                                        <  2:1268554/100‑1 (MQ=255)
      ccGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATg                                                                                        <  2:53214/100‑1 (MQ=255)
       cGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATgg                                                                                       <  2:1166803/100‑1 (MQ=255)
         aGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGa                                                                                     <  2:2197536/100‑1 (MQ=255)
            gCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAgg                                                                                  >  2:185824/1‑100 (MQ=255)
            gCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAgg                                                                                  <  1:1259594/100‑1 (MQ=255)
             cATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGt                                                                                 <  2:491045/100‑1 (MQ=255)
             cATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGt                                                                                 >  2:182005/1‑100 (MQ=255)
              aTCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAgg                                                                                  <  2:1995899/98‑1 (MQ=255)
               tCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTa                                                                               <  2:1075757/100‑1 (MQ=255)
                cGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTac                                                                              <  2:1177156/100‑1 (MQ=255)
                 gTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGCGGTACAGGGCACGGCATGGGAAGGTTaca                                                                             >  1:1018347/1‑100 (MQ=255)
                  tCACGGATATGCACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTacac                                                                            >  1:2070882/1‑100 (MQ=255)
                     cGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCaa                                                                         >  1:2581797/1‑100 (MQ=255)
                      ggATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCaaa                                                                        >  1:471734/1‑100 (MQ=255)
                         tatGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGCTACACCAAACTc                                                                     >  1:681263/1‑100 (MQ=255)
                          atGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATcc                                                                    >  2:1071521/1‑100 (MQ=255)
                          atGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATcc                                                                    >  1:63851/1‑100 (MQ=255)
                          atGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATcc                                                                    <  2:2600906/100‑1 (MQ=255)
                           tGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATccc                                                                   >  2:203027/1‑100 (MQ=255)
                           tGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATccc                                                                   >  1:2393850/1‑100 (MQ=255)
                             aaCTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCa                                                                  <  2:1558374/99‑1 (MQ=255)
                               cTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAAcc                                                               <  2:2324531/100‑1 (MQ=255)
                                 aTGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTg                                                             <  2:1515997/100‑1 (MQ=255)
                                 aTGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTg                                                             <  1:1714253/100‑1 (MQ=255)
                                  tGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTgg                                                            <  1:636269/100‑1 (MQ=255)
                                  tGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTgg                                                            <  2:1025868/100‑1 (MQ=255)
                                  tGACAATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGCAGGTTACACCAAAATCCCAACCTgg                                                            >  1:1318039/1‑100 (MQ=255)
                                   gACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGa                                                           >  2:1029146/1‑100 (MQ=255)
                                     cGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTgg                                                            <  1:633225/97‑1 (MQ=255)
                                     cGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATc                                                         <  2:2630095/100‑1 (MQ=255)
                                     cGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATc                                                         >  1:2343324/1‑100 (MQ=255)
                                     cGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATc                                                         <  1:1381520/100‑1 (MQ=255)
                                      gttACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCa                                                        <  1:1771452/98‑1 (MQ=255)
                                      gATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCa                                                        <  1:2872483/100‑1 (MQ=255)
                                       aTACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCAt                                                       >  1:2781470/1‑100 (MQ=255)
                                          ccGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCa                                                    >  1:2535293/1‑100 (MQ=255)
                                          ccGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCa                                                    >  1:1415439/1‑100 (MQ=255)
                                           cGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCaa                                                   >  1:1480988/1‑100 (MQ=255)
                                            gTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAg                                                  >  2:170858/1‑100 (MQ=255)
                                            gTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAg                                                  <  2:1620893/100‑1 (MQ=255)
                                            gTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAg                                                  <  2:2897172/100‑1 (MQ=255)
                                            gTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAg                                                  >  2:395111/1‑100 (MQ=255)
                                             ttCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAgg                                                 <  1:259568/100‑1 (MQ=255)
                                              tCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGc                                                <  2:783253/100‑1 (MQ=255)
                                              tCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGc                                                <  2:532485/100‑1 (MQ=255)
                                                gCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTa                                              >  2:1340886/1‑100 (MQ=255)
                                                gCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTa                                              >  2:1727477/1‑100 (MQ=255)
                                                gCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTa                                              <  2:1504086/100‑1 (MQ=255)
                                                gCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTa                                              >  1:1158963/1‑100 (MQ=255)
                                                  cTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTAc                                             <  1:368740/99‑1 (MQ=255)
                                                    gACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCa                                          >  1:2045284/1‑100 (MQ=255)
                                                     aCCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATGCCAACGTGGATCATGCAAGGCTagggaa                                         >  1:2187256/1‑95 (MQ=255)
                                                     aCCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCaa                                         >  1:2364685/1‑100 (MQ=255)
                                                        aTGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAAccc                                      <  1:2165652/100‑1 (MQ=255)
                                                          gCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTg                                    <  2:1061438/100‑1 (MQ=255)
                                                            aaCACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGc                                  <  1:1675935/100‑1 (MQ=255)
                                                             acacGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCa                                 <  1:1627969/100‑1 (MQ=255)
                                                              cacGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAg                                <  2:1045371/100‑1 (MQ=255)
                                                              cacGCGCAGCAGCAAGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAACATCCCAACCTGGATCAGGCAAGGCTACGCAACCCTGGc                                  >  1:1317683/1‑98 (MQ=255)
                                                               acGCGCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGa                               >  1:2481783/1‑100 (MQ=255)
                                                                cgcgCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGa                               <  1:182004/99‑1 (MQ=255)
                                                                cgcgCAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGAt                              >  1:416825/1‑100 (MQ=255)
                                                                 gcgcAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATg                             >  2:112899/1‑100 (MQ=255)
                                                                 gcgcAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATg                             <  1:747685/100‑1 (MQ=255)
                                                                  cgcAGCAGCACGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGa                            >  2:674613/1‑100 (MQ=255)
                                                                     agcagcaCGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCATGGCTACGCAACCCTGGCAGATGAAGc                         <  2:1773767/100‑1 (MQ=255)
                                                                        agcaCGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGGTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGt                      <  1:1484080/100‑1 (MQ=255)
                                                                         gcaCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTc                     >  2:810977/1‑100 (MQ=255)
                                                                           aCGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGa                   >  1:1583358/1‑100 (MQ=255)
                                                                            cGGCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAg                  >  2:452286/1‑100 (MQ=255)
                                                                            cGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAg                  <  1:194429/100‑1 (MQ=255)
                                                                              gCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCa                <  2:23232/100‑1 (MQ=255)
                                                                              gCTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGAACATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCa                >  2:716825/1‑100 (MQ=255)
                                                                               cTGGGAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCaa               >  2:2465660/1‑100 (MQ=255)
                                                                                 gggAAGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAAt             >  2:1632558/1‑100 (MQ=255)
                                                                                    aaGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCg          <  1:595727/100‑1 (MQ=255)
                                                                                    aaGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCg          <  2:1023354/100‑1 (MQ=255)
                                                                                     aGTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGt         >  2:1442834/1‑100 (MQ=255)
                                                                                      gTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTg        <  2:1153631/100‑1 (MQ=255)
                                                                                      gTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTg        <  1:2570646/100‑1 (MQ=255)
                                                                                      gTGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGAAGATGAAGCCGTCGAGCAAATGCGTg        >  1:2667335/1‑100 (MQ=255)
                                                                                       tGGTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGa       >  2:1108430/1‑100 (MQ=255)
                                                                                        ggTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCGACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGaa      >  1:1456393/1‑100 (MQ=255)
                                                                                        ggTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCCACCCTCGCAGATGAAGCCGTCCAGCAAATGCGTGa       >  1:2024090/1‑99 (MQ=255)
                                                                                        ggTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGaa      >  1:1017783/1‑100 (MQ=255)
                                                                                        ggTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGaa      >  1:1519268/1‑100 (MQ=255)
                                                                                        ggTACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGaa      <  1:1936594/100‑1 (MQ=255)
                                                                                          tACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGAAAt    >  1:509789/1‑100 (MQ=255)
                                                                                          tACAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGAAAt    <  1:1133726/100‑1 (MQ=255)
                                                                                           aCAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGAAATg   <  2:1762976/100‑1 (MQ=255)
                                                                                            cAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGAAATgg  >  2:668033/1‑100 (MQ=255)
                                                                                            cAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGAAATgg  >  1:2158598/1‑100 (MQ=255)
                                                                                            cAGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCAGGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGAAATgg  <  1:245585/100‑1 (MQ=255)
                                                                                             aGGGCACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGAAATgg  >  2:1266741/1‑99 (MQ=255)
                                                                                                |                                                                                               
TCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGAAATGG  >  NC_000913/3007979‑3008170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A