Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A9 F255 I0 R1
|
121 |
91.8 |
4500463 |
95.1% |
4279940 |
99.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
3,028,955 |
C→T |
28.8% |
V20I (GTA→ATA) |
ygfS ← |
putative 4Fe‑4S ferredoxin‑type oxidoreductase subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,028,955 | 0 | C | T | 28.8%
| 104.4
/ 49.0
| 73 | V20I (GTA→ATA) | ygfS | putative 4Fe‑4S ferredoxin‑type oxidoreductase subunit |
| Reads supporting (aligned to +/- strand): ref base C (22/30); new base T (12/9); total (34/39) |
| Fisher's exact test for biased strand distribution p-value = 3.05e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.26e-01 |
CCGGCGCACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCGTATCAAACAGAGTTAACATATCGCGCG > NC_000913/3028856‑3029052
|
ccGGCGCACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATGGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTAc > 2:1334480/1‑100 (MQ=255)
cGGCGCACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACt < 2:1677163/100‑1 (MQ=255)
gcgcACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTATTTc > 2:1327888/1‑100 (MQ=255)
gcgcACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTc < 1:1467613/100‑1 (MQ=255)
cACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTcaca < 1:2049511/100‑1 (MQ=255)
cACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTcaca > 1:143674/1‑100 (MQ=255)
cACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTcaca < 1:1869057/100‑1 (MQ=255)
aCTGATGCTGTCCAGTCGCGGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTATTTCACaa < 1:555103/100‑1 (MQ=255)
tGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGt < 1:1388058/100‑1 (MQ=255)
gATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTa > 1:714695/1‑100 (MQ=255)
gATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGAAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTa < 2:2206021/100‑1 (MQ=255)
tGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTATTTCACAAGTACGGCa > 1:1182735/1‑100 (MQ=255)
tGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCa < 1:43305/100‑1 (MQ=255)
tGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCa < 2:1602993/100‑1 (MQ=255)
gTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTATTTCACAAGTACGGCAg > 2:1798813/1‑100 (MQ=255)
gTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTATTTCACAAGTACGGCAg < 1:623868/100‑1 (MQ=255)
gTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTATTTCACAAGTACGGCAg > 1:854962/1‑100 (MQ=255)
ccAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTATTTCACAAGTACGGCAGc > 1:335708/1‑99 (MQ=255)
cAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCa < 2:63370/100‑1 (MQ=255)
aGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCaa > 2:1518232/1‑100 (MQ=255)
gTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAAt > 1:1197361/1‑100 (MQ=255)
cGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATAc > 1:1524493/1‑100 (MQ=255)
cGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATAc > 1:1659166/1‑100 (MQ=255)
gCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTATTTCACAAGTACGGCAGCCAATACa < 1:1502301/100‑1 (MQ=255)
cTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTATTTCACAAGTACGGCAGCCAATACAg < 2:1182731/100‑1 (MQ=255)
ccTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGc > 2:935272/1‑100 (MQ=255)
ttCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCg > 2:499047/1‑100 (MQ=255)
ttCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCg < 2:2091104/100‑1 (MQ=255)
tCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCgg < 2:860245/100‑1 (MQ=255)
cAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCgg < 1:466521/99‑1 (MQ=255)
gCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGAtt > 2:105957/1‑100 (MQ=255)
ggggTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTATTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAAc < 1:341605/100‑1 (MQ=255)
ggggTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAAc < 2:407201/100‑1 (MQ=255)
ggTAAAAAGACATCGGCATTCAACTCCTGTGCTGAAGGGTGAGCGACCACACAGGCTATTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTaa > 2:1305806/1‑97 (MQ=255)
aaaaaGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGTCCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATaa < 1:2123468/100‑1 (MQ=255)
aaaaaGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATCCCGTCAGCCGGATTCACGATaa > 1:967189/1‑100 (MQ=255)
aaGACAACGGCATTCACCTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTa < 1:752837/100‑1 (MQ=255)
aGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGCCCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTaa < 2:2000600/100‑1 (MQ=255)
gACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACAGAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAAc < 1:687294/100‑1 (MQ=255)
tCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTATTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGAtt > 2:704531/1‑100 (MQ=255)
tCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGAtt < 1:105957/100‑1 (MQ=255)
tCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGAtt < 2:29108/100‑1 (MQ=255)
aTTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTtcatc > 1:1909304/1‑100 (MQ=255)
cAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTATTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACg > 2:931902/1‑100 (MQ=255)
cAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTtcatca > 1:711429/1‑98 (MQ=255)
cAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACg > 2:1935533/1‑100 (MQ=255)
aCTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTATTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGAt > 2:1229264/1‑100 (MQ=255)
aCTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTATTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGAt < 1:902929/100‑1 (MQ=255)
aCTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGAt < 2:2167203/100‑1 (MQ=255)
cTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATg > 2:631015/1‑100 (MQ=255)
cTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATg > 1:787940/1‑100 (MQ=255)
cTGTTCTGAAGGGTGAGCGACCACACAGGCTATTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCt > 2:1481608/1‑100 (MQ=255)
cTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCt < 1:396345/100‑1 (MQ=255)
cTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCACCACGATGCCt > 2:191093/1‑100 (MQ=255)
gTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTc > 2:2037027/1‑100 (MQ=255)
tGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACTATTTCATCACGATGCCTTCGTAt < 2:494391/100‑1 (MQ=255)
tGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCGTa < 1:219213/99‑1 (MQ=255)
tGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCGTAt > 2:1191453/1‑100 (MQ=255)
gAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCGTATc > 2:732126/1‑100 (MQ=255)
gAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCGTATc < 1:1965114/100‑1 (MQ=255)
gAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCGTATc < 1:1964945/100‑1 (MQ=255)
aGGGTGAGCGACCACACAGGCTATTTCACAAGTAAGGCAGCCAATACAGTCAGCCGGATTAACGAGAATTAACGATTTCATTACGATGCCTTCGTATCaa < 1:467164/100‑1 (MQ=255)
ggTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCGTATCaa > 2:1400822/1‑98 (MQ=255)
ggTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCGTATCAAAc < 1:582693/100‑1 (MQ=255)
gTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCGTATCAAACa > 2:1748574/1‑100 (MQ=255)
gTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCGTATCAAACa > 1:1735395/1‑100 (MQ=255)
gAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCGTATCAAACaga < 2:2099004/100‑1 (MQ=255)
gCGACCACACAGGCTATTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCGTATCAAACagag > 1:2167794/1‑99 (MQ=255)
gACCACACAGGCTATTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCGTATCAAACAGAGTTa < 2:854962/100‑1 (MQ=255)
ccACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACCATTTCATCACGATGCCTTCGTATCAAACAGAGTTAAc < 2:1197357/100‑1 (MQ=255)
cacaGGCTATTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCGTATCAAACAGAGTTAACata < 1:1639183/100‑1 (MQ=255)
acaGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCGTATCAAACAGAGTTAACatat < 1:2028859/100‑1 (MQ=255)
ggCTATTTCAAAAGTCCGGCAGAAAATACAGTCAGCAGGATCAACCATAATTAACGATTTCAGCACGATGCCTTCGTATCAAACAGAGTTAACatacctc > 2:411776/1‑96 (MQ=255)
gCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCGTATCAAACAGAGTTAACATATcg < 2:1822896/98‑1 (MQ=255)
tccTTCACAAGTAGGGCAGCCAATACAGTCAGCCGGATTAACAATAATTAACGATTTCATCACGATGCCTTCGTATCAAACAGAGTTAACATATcgcgcg > 1:1516925/3‑100 (MQ=255)
cTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCGTATAAAACAGAGTTAATATATcgag > 1:654707/1‑94 (MQ=255)
|
CCGGCGCACTGATGCTGTCCAGTCGCTGTACCTTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCGTATCAAACAGAGTTAACATATCGCGCG > NC_000913/3028856‑3029052
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
