Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F183 I0 R1 119 96.4 4778198 94.0% 4491506 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,352,673 T→C 100% E118G (GAA→GGA)  arcB ← aerobic respiration control sensor histidine protein kinase, cognate to two‑component response regulators ArcA and RssB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,352,6730TC98.2% 363.1 / ‑2.9 113E118G (GAA→GGA) arcBaerobic respiration control sensor histidine protein kinase, cognate to two‑component response regulators ArcA and RssB
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base C (47/61);  total (48/65)
Fisher's exact test for biased strand distribution p-value = 5.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.50e-01

Only 100 of 115 total aligned reads displayed.

GAGCTGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTTCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGACAGAC  >  NC_000913/3352576‑3352769
                                                                                                 |                                                                                                
gAGCTGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTccc                                                                                                >  2:1261952/1‑100 (MQ=255)
 aGCTGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCt                                                                                               >  1:96932/1‑100 (MQ=255)
 aGCTGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCt                                                                                               <  1:192500/100‑1 (MQ=255)
  gCTGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTg                                                                                              <  2:822378/100‑1 (MQ=255)
  gCTGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTg                                                                                              <  2:822056/100‑1 (MQ=255)
   cTGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGa                                                                                             >  2:1326223/1‑100 (MQ=255)
    tGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTTGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCGCGAACGGCAATTCCCTg                                                                                              <  2:1217555/98‑1 (MQ=255)
    tGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGAt                                                                                            <  1:1074722/100‑1 (MQ=255)
    tGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGGTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGa                                                                                             <  2:1319673/99‑1 (MQ=255)
        tttGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTTCCTGATTTAg                                                                                        <  2:277663/100‑1 (MQ=255)
        tttGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGCTTTAg                                                                                        >  1:1527745/1‑100 (MQ=255)
         ttGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGc                                                                                       <  2:248613/100‑1 (MQ=255)
           gtgtCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTg                                                                                     <  2:2008493/100‑1 (MQ=255)
              tctcTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGc                                                                                  <  2:2079512/100‑1 (MQ=255)
               ctctTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCa                                                                                 <  2:154283/100‑1 (MQ=255)
               ctctTCGCGCTCTTTGATGTCGATTTTCAGTTGGCCGAAGGTTTCTTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTACCTGATTTACCTGGGc                                                                                  <  2:1559748/99‑1 (MQ=255)
                 ctTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAat                                                                               >  1:1631220/1‑100 (MQ=255)
                 ctTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAat                                                                               >  1:162452/1‑100 (MQ=255)
                 ctTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAat                                                                               >  1:1227296/1‑100 (MQ=255)
                 ctTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAat                                                                               >  1:2167495/1‑100 (MQ=255)
                  ttCGCGCTCTTTGATTTCAATTTTCAGTTTGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAata                                                                              <  2:1609351/100‑1 (MQ=255)
                  ttCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCCGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAata                                                                              >  2:1319284/1‑100 (MQ=255)
                  ttCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAata                                                                              <  2:1065705/100‑1 (MQ=255)
                   tCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAatat                                                                             <  2:460379/100‑1 (MQ=255)
                        ctctTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTCGGCAATATTATCt                                                                        >  2:1134752/1‑100 (MQ=255)
                         tctTTGTTTTCAATTTTCAGTTGTGCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCt                                                                        <  2:2284631/99‑1 (MQ=255)
                          ctTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCGATTCCCTGATTTAGCTGGGCAATATTATCttt                                                                      >  2:921935/1‑100 (MQ=255)
                          ctTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCttt                                                                      >  1:1982632/1‑100 (MQ=255)
                          ctTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCttt                                                                      >  1:1648223/1‑100 (MQ=255)
                           tttGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCtttt                                                                     <  1:1046790/100‑1 (MQ=255)
                           tttGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCtttt                                                                     <  1:379011/100‑1 (MQ=255)
                           tttGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCtttt                                                                     <  2:1474855/100‑1 (MQ=255)
                            ttGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTa                                                                    <  1:1952588/100‑1 (MQ=255)
                            ttGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTa                                                                    <  2:318763/100‑1 (MQ=255)
                              gATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAAc                                                                  <  2:2177402/100‑1 (MQ=255)
                              gATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAAc                                                                  <  1:1066767/100‑1 (MQ=255)
                               aTTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACt                                                                 <  2:1289093/100‑1 (MQ=255)
                                tttCAATTTTCAGTTGGCCGAAGGTTTCATGCAGTTCTGCTTCCGGTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTg                                                                >  2:1145535/1‑100 (MQ=255)
                                  tCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGaa                                                              >  1:796221/1‑100 (MQ=255)
                                  tCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCATGATTTTGCTGGGCAATATTATCTTTTAACt                                                                 >  2:1983563/1‑97 (MQ=255)
                                   cAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGa                                                               >  2:1999229/1‑98 (MQ=255)
                                    aaTTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACg                                                            >  2:1290385/1‑100 (MQ=255)
                                       tttCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGACCGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTg                                                         >  1:1443904/1‑100 (MQ=255)
                                       tttCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTg                                                         <  2:2284164/100‑1 (MQ=255)
                                        ttCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGa                                                        <  1:1010408/100‑1 (MQ=255)
                                         tCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAg                                                       <  2:796219/100‑1 (MQ=255)
                                          cAGTTTGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTAAgg                                                      <  1:1057744/100‑1 (MQ=255)
                                          cAGTTGGCCGGAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAgg                                                      <  1:638380/100‑1 (MQ=255)
                                           aGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGc                                                     >  2:1506736/1‑100 (MQ=255)
                                           aGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGc                                                     <  2:1648857/100‑1 (MQ=255)
                                           aGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGc                                                     <  1:2364550/100‑1 (MQ=255)
                                            gTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCt                                                    <  2:180741/100‑1 (MQ=255)
                                              tGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCa                                                  >  2:1153140/1‑100 (MQ=255)
                                               ggCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTTTTTTTTTTTTATACCCTGATTTAGCTGGGCAATATTATTTTTTAACTGAACGTTGAgg                                                      >  2:1685582/1‑95 (MQ=255)
                                                gCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCaaa                                                >  1:379462/1‑100 (MQ=255)
                                                 ccGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAt                                               >  1:1893742/1‑100 (MQ=255)
                                                 ccGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAt                                               >  2:476166/1‑100 (MQ=255)
                                                 ccGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAt                                               >  2:1911192/1‑100 (MQ=255)
                                                   gAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAtcg                                             <  1:443178/100‑1 (MQ=255)
                                                   gAAGATTTGCTGTAGTTCTAGTTCCGGTTTTTCAATAACTGTAATTGCCAGTTTTAGCTGGGCAATATTATGTTTTAACTGCACGTGGAGGCTCAAAtcg                                             <  2:381518/100‑1 (MQ=255)
                                                     aGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAtcgcg                                           <  2:255548/100‑1 (MQ=255)
                                                      ggTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAtcgcgc                                          <  2:2014412/100‑1 (MQ=255)
                                                       gTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAtcgcgct                                         >  1:1596321/1‑100 (MQ=255)
                                                        tttCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAtcgcgctc                                        >  1:671671/1‑100 (MQ=255)
                                                         ttCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAtcgcgctcg                                       <  2:569149/100‑1 (MQ=255)
                                                            cTGCAGTTCTGCTTCCGTATTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATCATCTTTTAACGGAACGTCGAGGCTCAAAttgcgccagcgc                                    <  2:1373804/100‑1 (MQ=255)
                                                             tGCAGTTCTGCTTGCGCTTTTTCACGAACGGAAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCa                                   >  1:1604573/1‑100 (MQ=255)
                                                             tGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCa                                   <  1:2168281/100‑1 (MQ=255)
                                                             tGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCa                                   <  1:929130/100‑1 (MQ=255)
                                                              gCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTTTTTTTTTTTTTTTTTTTTATCTTTTAACTGAACGTTGAGGCTCAAAtcgcgctcgcgc                                    >  2:1762588/1‑98 (MQ=255)
                                                              gCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCAt                                  >  1:891206/1‑100 (MQ=255)
                                                               cAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCAtc                                 <  1:582029/100‑1 (MQ=255)
                                                                aGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCAtct                                <  2:226544/100‑1 (MQ=255)
                                                                 gTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCAtctc                               >  1:1697916/1‑100 (MQ=255)
                                                                 gTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCAtctc                               >  1:1266150/1‑100 (MQ=255)
                                                                   tCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATctcct                             <  1:1027863/100‑1 (MQ=255)
                                                                   tCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATctcct                             >  1:1696426/1‑100 (MQ=255)
                                                                   tCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATctcc                              <  2:2044900/99‑1 (MQ=255)
                                                                    cTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATctcctc                            >  2:2171139/1‑100 (MQ=255)
                                                                     tGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCtcctcc                           >  1:1655470/1‑100 (MQ=255)
                                                                         tCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGtt                       <  1:32578/100‑1 (MQ=255)
                                                                         tCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGtt                       >  2:232572/1‑100 (MQ=255)
                                                                         tCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGtt                       >  2:1093972/1‑100 (MQ=255)
                                                                          ccGCTTTTTCACGAACGGCAATTTCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGttt                      <  1:482537/100‑1 (MQ=255)
                                                                           cGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGtttt                     >  1:1113028/1‑100 (MQ=255)
                                                                           cGCTTTTTCACGAACGGCAATTACCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACATTAAGGCTCAAATCGCCATCGCGCATctcct                             >  2:1983168/1‑92 (MQ=255)
                                                                            gCTTTTTCACGGACGGCAATTCCCTGATTTAGATGGGCAATATTATCTTTTAACTGATCGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGttttt                    <  1:747469/100‑1 (MQ=255)
                                                                            gCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCCCCTCCAGttttt                    <  2:1601047/100‑1 (MQ=255)
                                                                              tttttCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGc                  <  2:162428/100‑1 (MQ=255)
                                                                              tttttCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGc                  <  2:249139/100‑1 (MQ=255)
                                                                               ttttCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCa                 <  1:1841123/100‑1 (MQ=255)
                                                                                  tCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGTGCTCGCGCATCTCCTCCAGTTTTTGCACCa              >  1:1742349/1‑100 (MQ=255)
                                                                                       aaCGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGt         >  1:490215/1‑100 (MQ=255)
                                                                                        aCGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTg        <  2:1406473/100‑1 (MQ=255)
                                                                                        aCGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTg        <  2:1926027/100‑1 (MQ=255)
                                                                                         cGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGa       >  2:161642/1‑100 (MQ=255)
                                                                                          ggCAATTCTCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGAc      <  1:1031972/100‑1 (MQ=255)
                                                                                          ggCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGAc      <  1:232574/100‑1 (MQ=255)
                                                                                             aaTTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGACAGa   <  2:1326204/100‑1 (MQ=255)
                                                                                              aTTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGACAGAc  >  1:1913878/1‑100 (MQ=255)
                                                                                                 |                                                                                                
GAGCTGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTTCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGACAGAC  >  NC_000913/3352576‑3352769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A