Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F125 I0 R1 65 108.6 5319566 96.1% 5112102 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,352,673 T→C 100% E118G (GAA→GGA)  arcB ← aerobic respiration control sensor histidine protein kinase, cognate to two‑component response regulators ArcA and RssB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,352,6730TC97.5% 366.9 / ‑2.2 113E118G (GAA→GGA) arcBaerobic respiration control sensor histidine protein kinase, cognate to two‑component response regulators ArcA and RssB
Reads supporting (aligned to +/- strand):  ref base T (2/2);  new base C (49/56);  total (54/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

GAGCTGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTTCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGACA  >  NC_000913/3352576‑3352766
                                                                                                 |                                                                                             
gAGCTGAATTTGTGTCTCTTCGCGCTTTTTGATTTTAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTccc                                                                                             <  1:1398505/100‑1 (MQ=255)
  gCTGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTg                                                                                           <  1:1443293/100‑1 (MQ=255)
  gCTGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTg                                                                                           <  2:61394/100‑1 (MQ=255)
  gCTGAATTTGTGTCTCTTCGCACTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTg                                                                                           >  1:2567558/1‑100 (MQ=255)
    tGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGAt                                                                                         >  2:1833089/1‑100 (MQ=255)
    tGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGAt                                                                                         <  2:2024787/100‑1 (MQ=255)
    tGAATTTGTGTCTATTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCTGCTTTTTCACGAACGGCAATTACATGAt                                                                                         >  2:1281869/1‑100 (MQ=255)
      aaTTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGAttt                                                                                       >  2:764475/1‑100 (MQ=255)
       aTTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTACCTGATTTa                                                                                      >  1:1984435/1‑100 (MQ=255)
        tttGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAg                                                                                     >  1:107393/1‑100 (MQ=255)
         ttGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGc                                                                                    <  2:420809/100‑1 (MQ=255)
          tgtgTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCt                                                                                   <  1:275505/100‑1 (MQ=255)
           gtgtCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTg                                                                                  <  2:2253334/100‑1 (MQ=255)
            tgtCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTgg                                                                                 >  1:1164456/1‑100 (MQ=255)
            tgtCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTgg                                                                                 >  1:82936/1‑100 (MQ=255)
             gtCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTggg                                                                                <  1:398605/100‑1 (MQ=255)
             gtCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTgag                                                                                <  1:1798466/100‑3 (MQ=255)
             gtCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTNTCACGAACGGNAATTCCCTGNTTTAGCTggg                                                                                >  1:1272152/1‑100 (MQ=255)
              tctcTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGTAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGc                                                                               <  2:1441078/100‑1 (MQ=255)
              tctcTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGc                                                                               <  1:228972/100‑1 (MQ=255)
               ctctTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTTCTTCCGCTTTTTCACGAACGGCAATTTCCTGATTTAGCTGGGCa                                                                              >  2:595606/1‑100 (MQ=255)
               ctctTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCa                                                                              <  1:1296849/100‑1 (MQ=255)
                  ttCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAata                                                                           >  1:154473/1‑100 (MQ=255)
                  ttCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTACGCTTTTTAACGAACGGCAATTCCCTGATTTAGCTGGGCAata                                                                           >  1:871199/1‑100 (MQ=255)
                   tCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTACAGTTATACTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAatat                                                                          >  1:686039/1‑100 (MQ=255)
                     gcgcTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAatat                                                                          >  2:209094/1‑98 (MQ=255)
                      cgcTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTACCTGATTTAGCTGGGCAAtattat                                                                       >  1:1598754/1‑100 (MQ=255)
                          ctTTGATTTCAATTTTCAGTTGGCTGAAGGTTTCTTACAGTGCTGCTTGCGCTTTTTGATTAACGGCAATTCTCTGATTTAGTTGGGCTAGATTACCt                                                                     <  2:2183736/98‑1 (MQ=255)
                           tttGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCtttt                                                                  <  1:365828/100‑1 (MQ=255)
                            ttGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTa                                                                 <  2:2061828/100‑1 (MQ=255)
                             tGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTaa                                                                <  2:803725/100‑1 (MQ=255)
                              gATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTaan                                                               <  2:1272203/100‑2 (MQ=255)
                              gATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAAc                                                               <  1:277435/100‑1 (MQ=255)
                                  tCAATTTTCAGTTGGCCGAAGGTTACCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGCTTTAGCTGGGCAATATTATCTTTTAACTGaa                                                           >  2:1576276/1‑100 (MQ=255)
                                   cAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAAc                                                          <  1:953090/100‑1 (MQ=255)
                                   cAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAAc                                                          <  1:623209/100‑1 (MQ=255)
                                   cAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAAc                                                          >  1:25576/1‑100 (MQ=255)
                                   cAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAAc                                                          >  1:1353163/1‑100 (MQ=255)
                                   cAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAAc                                                          >  1:1410977/1‑100 (MQ=255)
                                   cAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAAc                                                          >  1:2131856/1‑100 (MQ=255)
                                   cAATTTTCAGTTGGCCGAAGGTTTCCTGAAGTTCTGCTTCCGCTTTTTCTCGAACGGCAATTACCTGATTTGGCTGGTCAATTTTGTGTTTTagc                                                               <  2:2482682/95‑3 (MQ=255)
                                     aTTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACg                                                         <  2:2509904/99‑1 (MQ=255)
                                       tttCAGTTGGCCGAATGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCGATTTCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTg                                                      <  2:2030029/100‑1 (MQ=255)
                                       tttCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTg                                                      <  2:2094028/100‑1 (MQ=255)
                                        ttCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGa                                                     <  2:840475/100‑1 (MQ=255)
                                        ttCACTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGGTGa                                                     >  2:204488/1‑100 (MQ=255)
                                           aGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACTGTAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGc                                                  <  2:1172040/100‑1 (MQ=255)
                                           aGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGc                                                  >  2:1732415/1‑100 (MQ=255)
                                           aGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGc                                                  <  1:2142842/100‑1 (MQ=255)
                                            gTTGGCCGAAGGTTTCCTGCTGTTCTGCTTCCGCTTTTTCACGAACGACAATTCACTGAATTAGCTGGACAATATTATCTTTTAACTGAACGTTGAGGCt                                                 >  1:1380111/1‑100 (MQ=255)
                                             ttGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTc                                                <  2:871190/100‑1 (MQ=255)
                                              tGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCa                                               <  2:1433834/100‑1 (MQ=255)
                                              tGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCa                                               >  1:1692141/1‑100 (MQ=255)
                                               ggCCGAAGGTTTCCTGCAGTTCTGCTCCCGGTTTTTCACGAACGGCAATTTCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCaa                                              >  2:1544468/1‑100 (MQ=255)
                                                    aaGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAtcgc                                         <  2:1007730/100‑1 (MQ=255)
                                                    aaGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAtcgc                                         <  2:1263930/100‑1 (MQ=255)
                                                    aaGGTTTCCTGCAGTTCTGC‑TCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAtcgcg                                        <  2:1085677/100‑1 (MQ=255)
                                                     aGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAtcgcg                                        <  1:2452997/100‑1 (MQ=255)
                                                      ggTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAtcgcgc                                       <  1:1356362/100‑1 (MQ=255)
                                                           ccTGCAGTTCTGCTTCCGCTTTTTCTCGAACGGCAATTTCCTGATTTAGCTGGGCAATATTATCTGTTAACTGAACGTTGAGGCTCAAAtcgcgctcgcg                                  <  1:2251743/100‑1 (MQ=255)
                                                            cTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAtcgcgctcgcgc                                 >  2:2583139/1‑100 (MQ=255)
                                                             tGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCa                                <  2:2338271/100‑1 (MQ=255)
                                                             tGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCa                                <  2:442940/100‑1 (MQ=255)
                                                             tGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCa                                >  2:904203/1‑100 (MQ=255)
                                                             tGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCa                                <  2:1655264/100‑1 (MQ=255)
                                                              gCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCAt                               >  1:962060/1‑100 (MQ=255)
                                                               cAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCAtc                              <  2:25576/100‑1 (MQ=255)
                                                                 gTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCAtctc                            >  1:1404643/1‑100 (MQ=255)
                                                                   tCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATctcct                          <  1:2358886/100‑1 (MQ=255)
                                                                      gCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCa                       >  1:1983269/1‑100 (MQ=255)
                                                                      gCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGAGCATTTTCTCCa                       >  1:1339066/1‑100 (MQ=255)
                                                                      gCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAAGCTCAAATCGCGCTCGCGCATCTCCTCCa                       >  1:646985/1‑100 (MQ=255)
                                                                        ttCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACATTGAGTCTCAAATCGCGCTCGCGCATCTCCTCCAGt                     >  1:1196612/1‑100 (MQ=255)
                                                                         tCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGtt                    >  1:2443447/1‑100 (MQ=255)
                                                                         tCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGtt                    >  1:313056/1‑100 (MQ=255)
                                                                          ccGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGACACAAATCGCACTCGCGCATCTCCTCCAGttt                   >  1:906654/1‑100 (MQ=255)
                                                                           cGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGtttt                  >  2:2492758/1‑100 (MQ=255)
                                                                           cGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTAAACATTGAGGCTCAAATCACGCTCGAGCATCTCCTCAAGtttt                  >  1:2160285/1‑100 (MQ=255)
                                                                            gCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGttttt                 >  1:1765276/1‑100 (MQ=255)
                                                                             cTTTTTCACGAACGGCAATTCCCTGATTTATCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTg                <  1:1706713/100‑1 (MQ=255)
                                                                             cTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTg                >  1:2001337/1‑100 (MQ=255)
                                                                             cTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTg                <  2:1765259/100‑1 (MQ=255)
                                                                               ttttCATGAACGGCAATTCCCTGTTTTAGCTGGGCAATATTATCTTTTAACTTAACGTTGAGGCTCAATTCGCGCTCTCGCATCTCCTCCAGTTTTTGCa              <  2:2236506/100‑1 (MQ=255)
                                                                               ttttCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTGGCGCATCTCCTCCAGTTTTTGCa              <  2:317969/100‑1 (MQ=255)
                                                                                 ttCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCAcc            >  1:2019333/1‑100 (MQ=255)
                                                                                 ttCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCAcc            <  1:1833106/100‑1 (MQ=255)
                                                                                  tCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCa           >  2:2307046/1‑100 (MQ=255)
                                                                                  tCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCa           <  2:2001318/100‑1 (MQ=255)
                                                                                  tCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCa           >  2:492085/1‑100 (MQ=255)
                                                                                     cGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGcc        >  1:460486/1‑100 (MQ=255)
                                                                                      gAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCg       <  2:154472/100‑1 (MQ=255)
                                                                                      gAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCg       >  2:286965/1‑100 (MQ=255)
                                                                                      gAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCg       <  2:833600/100‑1 (MQ=255)
                                                                                        aCGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCgag     <  1:1783278/100‑3 (MQ=255)
                                                                                        aCGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTg     <  2:422049/100‑1 (MQ=255)
                                                                                          ggCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTTAACGTTTAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGAc   <  1:2166396/100‑1 (MQ=255)
                                                                                          ggCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGAc   <  2:587475/100‑1 (MQ=255)
                                                                                          ggCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGAc   <  1:2112465/100‑1 (MQ=255)
                                                                                           gCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGACa  <  1:1889038/100‑1 (MQ=255)
                                                                                           gCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGACa  >  2:2404136/1‑100 (MQ=255)
                                                                                                 |                                                                                             
GAGCTGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTTCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGACA  >  NC_000913/3352576‑3352766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A