Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F191 I0 R1 105 142.7 6924153 96.8% 6702580 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,352,673 T→C 100% E118G (GAA→GGA)  arcB ← aerobic respiration control sensor histidine protein kinase, cognate to two‑component response regulators ArcA and RssB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,352,6730TC98.5% 458.3 / ‑4.0 129E118G (GAA→GGA) arcBaerobic respiration control sensor histidine protein kinase, cognate to two‑component response regulators ArcA and RssB
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base C (65/62);  total (67/62)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 132 total aligned reads displayed.

GAGCTGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTTCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGACAGA  >  NC_000913/3352576‑3352768
                                                                                                 |                                                                                               
gAGCTGACTTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTCCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTccc                                                                                               <  1:1858686/100‑1 (MQ=255)
gAGCTGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTccc                                                                                               >  2:773143/1‑100 (MQ=255)
 aGCTGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCt                                                                                              >  2:2031375/1‑100 (MQ=255)
  gCTGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTg                                                                                             <  2:1699062/100‑1 (MQ=255)
  gCTGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTg                                                                                             <  1:1005211/100‑1 (MQ=255)
   cTGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGa                                                                                            >  2:993453/1‑100 (MQ=255)
    tGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGAt                                                                                           >  2:2819384/1‑100 (MQ=255)
    tGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGAt                                                                                           >  1:2793063/1‑100 (MQ=255)
    tGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGAt                                                                                           >  2:3225409/1‑100 (MQ=255)
    tGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGAt                                                                                           <  1:2342057/100‑1 (MQ=255)
    tGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGAt                                                                                           >  2:403742/1‑100 (MQ=255)
     gAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGAtt                                                                                          <  2:2793085/100‑1 (MQ=255)
       gatcttgtCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTa                                                                                        <  2:1580770/95‑1 (MQ=255)
         ttGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGc                                                                                      <  1:488187/100‑1 (MQ=255)
         ttGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGGTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGc                                                                                      >  1:1241698/1‑100 (MQ=255)
          tgtgTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCt                                                                                     >  2:1279134/1‑100 (MQ=255)
            tgtCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTaagatc                                                                                   >  1:1580755/1‑95 (MQ=255)
            tgtCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTgg                                                                                   >  1:2805808/1‑100 (MQ=255)
              tctcTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGc                                                                                 >  1:1780992/1‑100 (MQ=255)
              tctcTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGc                                                                                 <  2:2146061/100‑1 (MQ=255)
               ctctTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCa                                                                                <  2:624680/100‑1 (MQ=255)
                tctTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCaa                                                                               >  2:52526/1‑100 (MQ=255)
                 ctTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAat                                                                              >  1:1525813/1‑100 (MQ=255)
                    cgcgCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAAtatt                                                                           >  1:2655540/1‑100 (MQ=255)
                    cgcgCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAAtatt                                                                           >  2:2586796/1‑100 (MQ=255)
                     gcgcTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAAtatta                                                                          <  2:1431282/100‑1 (MQ=255)
                       gcTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATTTTATc                                                                        >  1:2634997/1‑100 (MQ=255)
                         tctTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCtt                                                                      <  2:2323986/100‑1 (MQ=255)
                         tctTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCtt                                                                      <  2:2157165/100‑1 (MQ=255)
                          ctTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGCACGCCAATTCCCTGATTTAGCTGGGCAATATTATCttt                                                                     >  2:2121020/1‑100 (MQ=255)
                           tttGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCtttt                                                                    <  1:1915039/100‑1 (MQ=255)
                           tttGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCtttt                                                                    <  2:3418439/100‑1 (MQ=255)
                            ttGATTTTAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCttt                                                                     <  1:455158/98‑1 (MQ=255)
                            ttGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTa                                                                   <  1:1328200/100‑1 (MQ=255)
                              gATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAAc                                                                 <  2:1540666/100‑1 (MQ=255)
                              gATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAAc                                                                 <  2:2774828/100‑1 (MQ=255)
                              gATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAAc                                                                 <  2:2263239/100‑1 (MQ=255)
                              gATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAAc                                                                 <  1:249504/100‑1 (MQ=255)
                               aTTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACt                                                                <  2:2072263/100‑1 (MQ=255)
                               aTTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACt                                                                <  1:1732715/100‑1 (MQ=255)
                                tttCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTg                                                               <  2:167936/100‑1 (MQ=255)
                                  tCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGaa                                                             <  2:1128131/100‑1 (MQ=255)
                                  tCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGaa                                                             >  1:2561914/1‑100 (MQ=255)
                                   cAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCAGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAAc                                                            <  1:1830027/100‑1 (MQ=255)
                                     aTTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGt                                                          >  2:984583/1‑100 (MQ=255)
                                           aGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGc                                                    <  2:2726604/100‑1 (MQ=255)
                                           aGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGc                                                    <  2:1436524/100‑1 (MQ=255)
                                           aGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGc                                                    <  1:266780/100‑1 (MQ=255)
                                             ttGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTc                                                  <  1:1052538/100‑1 (MQ=255)
                                              tggatgAAGGTTTCCTACAGGTCAGCTTCCGTTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAgg                                                     <  1:166315/91‑1 (MQ=255)
                                              tGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCa                                                 >  2:2100998/1‑100 (MQ=255)
                                              tGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTATTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTg                                                        <  1:2055160/93‑1 (MQ=255)
                                               ggCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCaa                                                >  2:1400527/1‑100 (MQ=255)
                                                gCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCaaa                                               >  2:672663/1‑100 (MQ=255)
                                                gCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCaaa                                               >  2:1884329/1‑100 (MQ=255)
                                                 ccGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAt                                              >  2:1275012/1‑100 (MQ=255)
                                                   gAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCCATATTATCTTTTAACTGAACGTTGAGGCTCAAAtcg                                            >  2:1043394/1‑100 (MQ=255)
                                                    aaGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAtcgc                                           >  1:1344744/1‑100 (MQ=255)
                                                    aaGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAtcgc                                           <  2:29094/100‑1 (MQ=255)
                                                          tCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAtcgcgctcgc                                     <  1:2932889/100‑1 (MQ=255)
                                                          tCCTGCAGTTCTGCTTCCGCTTTTTAACGAACGACAATTCCCTGATTTAGCTCGGCAATATTATCTTTTCACTGAGCGTTGAGGCTCAAAtcgcgctcgc                                     >  1:1536772/1‑100 (MQ=255)
                                                           ccTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAtcgcgctcgcg                                    <  2:1018656/100‑1 (MQ=255)
                                                           ccTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAtcgcgctcgcg                                    >  2:1540829/1‑100 (MQ=255)
                                                            cTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAtcgcgctcgcg                                    >  2:941078/1‑99 (MQ=255)
                                                             tGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCa                                  >  2:1154477/1‑100 (MQ=255)
                                                             tGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCa                                  >  1:3286981/1‑100 (MQ=255)
                                                             tGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCa                                  <  1:468608/100‑1 (MQ=255)
                                                                aGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCGATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCAtct                               <  1:2580663/100‑1 (MQ=255)
                                                                aGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCAtct                               >  2:2740011/1‑100 (MQ=255)
                                                                 gTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCAtc                                <  1:2059629/98‑1 (MQ=255)
                                                                  ttCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATctcc                             <  1:1445486/100‑1 (MQ=255)
                                                                    cTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATctcctc                           >  1:252163/1‑100 (MQ=255)
                                                                    cTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATctcctc                           >  1:3445998/1‑100 (MQ=255)
                                                                     tGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCtcctcc                          >  1:1042634/1‑100 (MQ=255)
                                                                      gCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCa                         >  2:3163407/1‑100 (MQ=255)
                                                                          ccGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGttt                     >  1:3131539/1‑100 (MQ=255)
                                                                          ccGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGttt                     >  2:691106/1‑100 (MQ=255)
                                                                           cGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGtttt                    >  1:3051284/1‑100 (MQ=255)
                                                                            gCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGttttt                   <  1:1941053/100‑1 (MQ=255)
                                                                             cTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTg                  <  1:3415760/100‑1 (MQ=255)
                                                                             cTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTg                  <  1:773136/100‑1 (MQ=255)
                                                                              tttttCACGAACGGCAATTCCCTGATTTAGCTGGCCAATATTATCTTTTAACTGACCGTGGAGGCTAAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGc                 <  1:2780042/100‑1 (MQ=255)
                                                                                tttCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCAc               >  2:991638/1‑100 (MQ=255)
                                                                                tttCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCAc               <  1:2100981/100‑1 (MQ=255)
                                                                                 ttCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCAcc              <  1:748858/100‑1 (MQ=255)
                                                                                  tCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCa             >  2:301312/1‑100 (MQ=255)
                                                                                  tCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCa             <  2:467862/100‑1 (MQ=255)
                                                                                   cACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAg            >  1:163979/1‑100 (MQ=255)
                                                                                   cACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAg            <  2:1238365/100‑1 (MQ=255)
                                                                                     cGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGcc          >  2:1666921/1‑100 (MQ=255)
                                                                                     cGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGcc          >  2:1851183/1‑100 (MQ=255)
                                                                                        aCGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTg       >  1:2169125/1‑100 (MQ=255)
                                                                                        aCGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTg       <  1:2031358/100‑1 (MQ=255)
                                                                                        aCGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTg       <  2:2561933/100‑1 (MQ=255)
                                                                                          tccgatctCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGAc     <  2:2050724/93‑1 (MQ=255)
                                                                                          ggCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGACCGTTGCGGCTCAAATCGCGCCCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGAc     >  2:3319798/1‑100 (MQ=255)
                                                                                          ggCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGAc     <  1:1540814/100‑1 (MQ=255)
                                                                                           gCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGACa    >  1:891912/1‑100 (MQ=255)
                                                                                            cAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGACAg   >  2:657057/1‑100 (MQ=255)
                                                                                            cAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGACAg   >  2:1850913/1‑100 (MQ=255)
                                                                                                 |                                                                                               
GAGCTGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTTCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGACAGA  >  NC_000913/3352576‑3352768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A