Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F183 I0 R1 119 96.4 4778198 94.0% 4491506 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,337,024 G→A 100% S3L (TCG→TTG)  adiC ← arginine:agmatine antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,337,0240GA94.2% 266.8 / 1.6 88S3L (TCG→TTG) adiCarginine:agmatine antiporter
Reads supporting (aligned to +/- strand):  ref base G (3/3);  new base A (41/41);  total (44/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGG  >  NC_000913/4336933‑4337120
                                                                                           |                                                                                                
tGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATa                                                                                          >  1:1714933/1‑100 (MQ=255)
  cAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAAt                                                                                        <  1:1016351/100‑1 (MQ=255)
    ggTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTa                                                                                      >  1:1230268/1‑100 (MQ=255)
     gTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTaa                                                                                     >  2:574196/1‑100 (MQ=255)
      tAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTaaa                                                                                    >  1:350386/1‑100 (MQ=255)
      tAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTaaa                                                                                    >  1:949079/1‑100 (MQ=255)
       aaCAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAAc                                                                                   <  1:1658019/100‑1 (MQ=255)
        aCAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACt                                                                                  <  2:2326643/100‑1 (MQ=255)
         cAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTc                                                                                 >  1:427970/1‑100 (MQ=255)
         cAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTc                                                                                 >  1:1622427/1‑100 (MQ=255)
         cAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTc                                                                                 >  1:678884/1‑100 (MQ=255)
         cAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTc                                                                                 >  1:1279235/1‑100 (MQ=255)
          aGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTcc                                                                                <  1:413341/100‑1 (MQ=255)
           gAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCt                                                                               >  2:1310511/1‑100 (MQ=255)
              aaaCACGTGACTACATAATATTCCCCGACACCATCAGGGGGACGGGGATTAAGCCAACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCg                                                                            >  2:599264/1‑100 (MQ=255)
                acacCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGaa                                                                          >  2:899853/1‑100 (MQ=255)
                    cTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCg                                                                      <  2:760577/100‑1 (MQ=255)
                    cTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCg                                                                      <  1:539875/100‑1 (MQ=255)
                     tGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGa                                                                     >  2:831093/1‑100 (MQ=255)
                        ccccATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCt                                                                  >  1:865668/1‑100 (MQ=255)
                         cccATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCtt                                                                 >  1:741029/1‑100 (MQ=255)
                          ccATAATATTCCCCGACGCCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTc                                                                >  1:770068/1‑100 (MQ=255)
                           cATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCg                                                               <  1:99798/100‑1 (MQ=255)
                             taataTTCCCCGACACCGTCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTg                                                             >  1:1292279/1‑100 (MQ=255)
                             taataTTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTg                                                             >  1:1438952/1‑100 (MQ=255)
                             taataTTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTg                                                             >  1:1380078/1‑100 (MQ=255)
                             gatctttCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGATATAATTAAACTCCTGCGAAGGCGAGCTTCGTg                                                             <  1:2264983/95‑1 (MQ=255)
                              aataTTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCGCTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGa                                                            <  1:99374/100‑1 (MQ=255)
                              aataTTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGa                                                            >  1:1899715/1‑100 (MQ=255)
                               atatTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGAc                                                           <  2:1966841/100‑1 (MQ=255)
                               atatTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGAc                                                           <  2:1949878/100‑1 (MQ=255)
                                tatTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACa                                                          >  1:1154567/1‑100 (MQ=255)
                                tatTCCCCGAAACCATAAGGGTGACGCGGATTCAGCCATCTCTGTGAGCATCTGCATCCATAGACATATTTAACCTCCTGCGAAGGCGAGCTTCGTGAc                                                           <  2:978289/99‑1 (MQ=255)
                                  ttCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGagatc                                                        >  2:2264962/1‑96 (MQ=255)
                                  ttCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGt                                                              <  2:1354159/94‑1 (MQ=255)
                                  ttCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAAt                                                        <  1:1864303/100‑1 (MQ=255)
                                  ttCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAAt                                                        <  1:2271610/100‑1 (MQ=255)
                                  ttCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAAt                                                        >  2:2012484/1‑100 (MQ=255)
                                  atCCCCGTCACCCTGATGGTGTCGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAAt                                                        >  2:1220351/2‑100 (MQ=37)
                                    ccccGACACCATCAGGGTGACGGGGATTAAGCCCCCTATGTGCGCCTCAGCATCCAAAGATATAATTAAACTCTTGCGAAGGCGAGCTTCGTGACaa                                                         >  2:2027448/1‑97 (MQ=255)
                                     cccGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAAATCCTGCGACGGCGCGCTTCGTGACAATaa                                                      >  1:1387921/1‑99 (MQ=255)
                                     cccGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATaaa                                                     >  1:2111480/1‑100 (MQ=255)
                                         acacCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTAc                                                 <  2:1237093/100‑1 (MQ=255)
                                          cacCATCAGGGTGACGGGGATTAAGCCCACTTTGTTAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACg                                                <  2:650623/100‑1 (MQ=255)
                                          cacCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACg                                                <  1:91192/100‑1 (MQ=255)
                                          cacCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACg                                                <  2:171177/100‑1 (MQ=255)
                                           acCATCAGGGTGACGGGGATTAAGCCCACTTTGTGCGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGt                                               >  2:2132331/1‑100 (MQ=255)
                                           acCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGt                                               <  1:2167016/100‑1 (MQ=255)
                                                 aGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGtcatatc                                         <  2:1084182/100‑1 (MQ=255)
                                                 aGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGtcatatc                                         <  2:251191/100‑1 (MQ=255)
                                                 aGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGtcatatc                                         <  2:1537112/100‑1 (MQ=255)
                                                 aGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGtcatatc                                         >  2:1220671/1‑100 (MQ=255)
                                                  gggTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGtcatatca                                        >  2:259076/1‑100 (MQ=255)
                                                  gggTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGtcatatca                                        >  1:2045372/1‑100 (MQ=255)
                                                        cGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGcc                                  <  1:700158/100‑1 (MQ=255)
                                                        cGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGcc                                  >  2:1673014/1‑100 (MQ=255)
                                                          gggATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTg                                >  1:1776150/1‑100 (MQ=255)
                                                          gggATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTg                                <  2:706063/100‑1 (MQ=255)
                                                           ggATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGc                               <  1:1310518/100‑1 (MQ=255)
                                                             aTTAAGCCCTAGTTGTGAGCATGAGCATCCGCAGACATAATTAACCTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCGTACGCCTGCAt                             <  2:704161/100‑1 (MQ=255)
                                                             aTTAAGCCCACTTTGTGAGCTTGAGCATCCAAAGACATAATTAAACTCCTTCGAAGGCGAGCTGCGTGACAATAAATTACGTAATTTCATACCCCTg                                >  2:1624650/1‑97 (MQ=255)
                                                              ttAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCAtt                            >  1:471090/1‑100 (MQ=255)
                                                               tAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTc                           <  1:2066118/100‑1 (MQ=255)
                                                               tAAGCCCACTTTGTGAGAATCAGCATCCAAAGACATAATTAAAATCCTGCGAAGGCGAGGTTCGTGACAATAAATTACGTCATATCATgcgc                                   >  2:1674503/1‑92 (MQ=255)
                                                                 aGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGa                         >  2:1228350/1‑100 (MQ=255)
                                                                  gCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGAt                        <  2:1380070/100‑1 (MQ=255)
                                                                   cccACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATa                       >  2:233657/1‑100 (MQ=255)
                                                                     cACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGt                     >  1:133338/1‑100 (MQ=255)
                                                                       cTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTg                   <  1:465474/100‑1 (MQ=255)
                                                                       cTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTg                   <  2:1380574/100‑1 (MQ=255)
                                                                       cTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTg                   <  2:1155544/100‑1 (MQ=255)
                                                                        tttGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGa                  <  2:885909/100‑1 (MQ=255)
                                                                         ttttgAGCATCAGCATGCAAAGACATAATTAAACTCCTGCGAATGCTAGCTTCGGTACTATTAATTACTTCATATCATACGCCTGCATTCGATAGTTGaa                 <  1:1388520/97‑1 (MQ=255)
                                                                          tttgAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAat                <  2:885847/98‑1 (MQ=255)
                                                                          tgtgAGCATCAGCATCCAAAGACATAATTAATCTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAat                <  1:2251202/100‑1 (MQ=255)
                                                                          tgtgAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGGCATATCATACGCCTGCATTCGATAGTTGAat                <  2:1009097/100‑1 (MQ=255)
                                                                            tgAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAatta              <  1:644880/100‑1 (MQ=255)
                                                                             gAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAattat             >  1:113747/1‑100 (MQ=255)
                                                                             gAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAattat             <  2:1292272/100‑1 (MQ=255)
                                                                               gcatcagcatcCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAAttatta           >  2:349187/1‑100 (MQ=255)
                                                                               gcatcagcatcCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAAttatta           >  1:368220/1‑100 (MQ=255)
                                                                                  tcagcatcCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCg        <  1:591542/100‑1 (MQ=255)
                                                                                     gcatcCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGAcc     <  2:694664/100‑1 (MQ=255)
                                                                                     gcatcCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGAcc     <  1:2262627/100‑1 (MQ=255)
                                                                                      catcCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCg    <  1:1902483/100‑1 (MQ=255)
                                                                                      catcCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCg    >  2:954242/1‑100 (MQ=255)
                                                                                       atcCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCgg   <  1:1810295/100‑1 (MQ=255)
                                                                                        tcCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCggg  >  2:1516976/1‑100 (MQ=255)
                                                                                           |                                                                                                
TGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGG  >  NC_000913/4336933‑4337120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A