Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F110 I0 R1
|
60 |
98.0 |
4713732 |
96.7% |
4558178 |
99.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
4,337,024 |
G→A |
100% |
S3L (TCG→TTG) |
adiC ← |
arginine:agmatine antiporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,337,024 | 0 | G | A | 99.0%
| 273.3
/ ‑5.9
| 79 | S3L (TCG→TTG) | adiC | arginine:agmatine antiporter |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (33/45); minor base C (0/1); total (33/46) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 |
CAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGG > NC_000913/4336927‑4337120
|
cAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCaaa < 2:1759491/100‑1 (MQ=255)
aGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAg > 1:65097/1‑100 (MQ=255)
gTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGAc > 1:1167467/1‑100 (MQ=255)
gTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGAc < 1:900514/100‑1 (MQ=255)
gTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGAc < 1:604927/100‑1 (MQ=255)
gTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGAc < 1:2202686/100‑1 (MQ=255)
ttGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACAt < 2:147754/100‑1 (MQ=255)
ttGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACAt < 2:861005/100‑1 (MQ=255)
ggTAACAGAAAAGCACCTGACGCCATAATATTCCCCGACACCAGCAGGGTGACGCGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTa < 2:1979047/100‑1 (MQ=255)
ggTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTa > 1:1739675/1‑100 (MQ=255)
ggTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTa > 1:597858/1‑100 (MQ=255)
ggTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTa > 2:1628168/1‑100 (MQ=255)
gTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTaa < 2:1112104/100‑1 (MQ=255)
gTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTaa < 1:424250/100‑1 (MQ=255)
tAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTaaa > 1:2197558/1‑100 (MQ=255)
tAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTaaa > 1:579927/1‑100 (MQ=255)
aaCAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAAc < 2:2060411/100‑1 (MQ=255)
aCAGAAAAACACCTGACCCCATAATATTCCCCGACGCCAGCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACt < 2:1830156/100‑1 (MQ=255)
aGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTcc > 1:302589/1‑100 (MQ=255)
gAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCt > 1:1718012/1‑100 (MQ=255)
aaaaaCACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTg < 1:429926/100‑1 (MQ=255)
aaaCACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCg < 2:1041793/100‑1 (MQ=255)
cacCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAg < 1:800787/100‑1 (MQ=255)
acCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAgg < 1:1464134/100‑1 (MQ=255)
ccTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGc < 2:1936647/100‑1 (MQ=255)
tGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGa < 1:2208899/100‑1 (MQ=255)
ccccATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCt > 2:459362/1‑100 (MQ=255)
cccATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCACAATTTGTGAGCATCAGCATCCAAAGAAATAATTAAACTACTGCGAAGGCGAGCtt > 2:1869442/1‑100 (MQ=255)
ataataTTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGt < 1:1840680/100‑1 (MQ=255)
taataTTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTg < 2:1485113/100‑1 (MQ=255)
taataTTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTg > 2:1291552/1‑100 (MQ=255)
atatTCCCCGACACCATCAGGGTGACGGGGATTCAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGAc < 2:199174/100‑1 (MQ=255)
atatTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTTATCTCCTGCGAAGGCGAGCTTCg < 2:2220402/96‑1 (MQ=255)
atatTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGAc < 1:1548961/100‑1 (MQ=255)
atatTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGAc < 2:1591803/100‑1 (MQ=255)
atatTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGAc < 2:1818271/100‑1 (MQ=255)
tatTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACa < 1:1033283/100‑1 (MQ=255)
tatTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACa < 1:45963/100‑1 (MQ=255)
atTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACaa < 1:2273830/100‑1 (MQ=255)
ccccGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATaa > 2:64740/1‑100 (MQ=255)
cccGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATaaa > 1:1694934/1‑100 (MQ=255)
ccGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAAt > 2:149188/1‑100 (MQ=255)
gACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTa < 2:2326875/100‑1 (MQ=255)
acacCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTAc < 2:2333205/100‑1 (MQ=255)
cacCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACg > 2:1474114/1‑100 (MQ=255)
acCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGt < 2:2197539/100‑1 (MQ=255)
ccATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGtc > 1:4352/1‑100 (MQ=255)
ccATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGtc > 2:2101833/1‑100 (MQ=255)
cATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGtca < 2:71519/100‑1 (MQ=255)
cATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGtca > 2:1511040/1‑100 (MQ=255)
cATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGtca > 2:880127/1‑100 (MQ=255)
aGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGtcatatc < 2:752096/100‑1 (MQ=255)
aGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACAtcatatc > 2:490193/1‑100 (MQ=255)
gggTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGtcatatca > 2:982208/1‑100 (MQ=255)
cGGGGATTAAGCCCACTTTGTGAGCATCAGTATCCCAAGACATACTTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGcc < 1:106806/100‑1 (MQ=255)
cGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGcc > 2:599183/1‑100 (MQ=255)
gggATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTg < 2:1786295/100‑1 (MQ=255)
gggATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTg > 1:599646/1‑100 (MQ=255)
ttATGCGCACTTTGTATGCATCAGCATCCAAATACATAATTAAACCCCTGCGAAGGCGAGCTTTGTAACAATAAATTACGTCAGATCTTAGTCCTgt < 2:914836/97‑2 (MQ=255)
tAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTc < 1:492988/100‑1 (MQ=255)
aGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGa > 1:907707/1‑100 (MQ=255)
aGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGa > 1:843541/1‑100 (MQ=255)
aGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGACCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGa > 2:1506070/1‑100 (MQ=255)
cccACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATa < 1:1511671/100‑1 (MQ=255)
ccACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAg < 2:261186/100‑1 (MQ=255)
ccACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAg > 1:452539/1‑100 (MQ=255)
aCTTTGTGAGCATCAGCATCCAAAGACATCATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGtt < 1:1238287/100‑1 (MQ=255)
cTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTg < 1:887580/100‑1 (MQ=255)
cTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTg < 2:429786/100‑1 (MQ=255)
cTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTg < 1:297698/100‑1 (MQ=255)
tgtgAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAat < 1:588388/100‑1 (MQ=255)
tgtgAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAat < 1:1982606/100‑1 (MQ=255)
agcatcagcatcCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAattatt < 2:1227662/100‑1 (MQ=255)
gcatcagcatcCAAAGACATAATTAAACTCATGAGAAGGAGAGAATCGTGACAATACATTACCACCTATAATACGCCTGCATTCAATAGTTGAAtcatta > 2:437323/1‑100 (MQ=255)
tcagcatcCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCg < 2:1167462/100‑1 (MQ=255)
agcatcCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGAc < 2:1101577/100‑1 (MQ=255)
agcatcCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGAc > 1:1312742/1‑100 (MQ=255)
gcatcCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATAACATACGCCTGCATTCGATAGTTTAATTATTATTGAc > 2:2189799/1‑99 (MQ=255)
catcCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCg > 1:328145/1‑100 (MQ=255)
tcCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCggg > 2:2130327/1‑100 (MQ=255)
|
CAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGG > NC_000913/4336927‑4337120
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
