Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F244 I0 R1	270	114.4	5840133	93.3%	5448844	99.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,337,024	G→A	100%	S3L (TCG→TTG)	adiC ←	arginine:agmatine antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,337,024	0	G	A	99.2%	834.7 / ‑3.5	249	S3L (TCG→TTG)	adiC	arginine:agmatine antiporter
Reads supporting (aligned to +/- strand): ref base G (2/0); new base A (122/124); total (124/125)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

Only 100 of 249 total aligned reads displayed.

AGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGG  >  NC_000913/4336928‑4337120

aGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAg                                                                                               <  2:1155150/100‑1 (MQ=255)

aGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAg                                                                                               <  1:2694539/100‑1 (MQ=255)

  gTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGAc                                                                                             <  2:1322430/100‑1 (MQ=255)

       cAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAAt                                                                                        <  2:2910185/100‑1 (MQ=255)

         ggTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTa                                                                                      >  1:1024103/1‑100 (MQ=255)

         ggTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTa                                                                                      <  2:432346/100‑1 (MQ=255)

         ggTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTa                                                                                      >  2:1380729/1‑100 (MQ=255)

         ggTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTa                                                                                      <  1:909372/100‑1 (MQ=255)

              cAGAAAAACACCTGACCCCATAATATTCCCCAACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTc                                                                                 >  1:235631/1‑100 (MQ=255)

                gAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCt                                                                               >  2:1191670/1‑100 (MQ=255)

                gAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCAACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACt                                                                                  >  1:2112611/1‑97 (MQ=255)

                  aaaaCACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTcccg                                                                              <  2:2709572/99‑3 (MQ=255)

                  aaaaCACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGc                                                                             >  2:1204925/1‑100 (MQ=255)

                  aaaaCACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGc                                                                             <  2:1165386/100‑1 (MQ=255)

                   aaaCACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGc                                                                             <  1:2390249/99‑1 (MQ=255)

                      cacCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAg                                                                         <  1:1181868/100‑1 (MQ=255)

                      cacCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAg                                                                         >  1:494753/1‑100 (MQ=255)

                       acCTGACCCCATAATATTCCCCAACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAgg                                                                        <  2:235632/100‑1 (MQ=255)

                        ccTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGc                                                                       >  2:1486318/1‑100 (MQ=255)

                        ccTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGc                                                                       >  2:1600003/1‑100 (MQ=255)

                        ccTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGc                                                                       <  2:1897750/100‑1 (MQ=255)

                         cTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCg                                                                      <  2:2037009/100‑1 (MQ=255)

                         cTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCg                                                                      <  1:2195074/100‑1 (MQ=255)

                         cTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCAGCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCg                                                                      <  1:1123016/100‑1 (MQ=255)

                          tGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGa                                                                     <  1:2897756/100‑1 (MQ=255)

                           gACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGGCAAGGCGa                                                                     >  1:2169349/1‑99 (MQ=255)

                           gACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAg                                                                    <  2:1999421/100‑1 (MQ=255)

                            aCCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGc                                                                   <  2:2474025/100‑1 (MQ=255)

                            aCCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGc                                                                   <  2:1505884/100‑1 (MQ=255)

                             ccccATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCt                                                                  <  2:1670315/100‑1 (MQ=255)

                              cccATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCtt                                                                 >  1:1662950/1‑100 (MQ=255)

                                cATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCg                                                               >  2:2807901/1‑100 (MQ=255)

                                 ataataTTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGt                                                              <  2:300579/100‑1 (MQ=255)

                                 ataataTTCCCCGACACAATCGGGGTGACGGGGATTGAGCCAACTTTGTGAGCATGAGCATCGAAAAACATAATTAATCTACTGCGAAGGCGa                                                                     <  1:2413522/93‑1 (MQ=255)

                                  taataTTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTg                                                             <  1:1786692/100‑1 (MQ=255)

                                  gcttaatCCCCGTCACCCTGATGGTGTCGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTg                                                             >  2:708480/7‑100 (MQ=25)

                                   aataTTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGa                                                            <  2:665210/100‑1 (MQ=255)

                                   aataTTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGa                                                            <  1:2028087/100‑1 (MQ=255)

                                    atatTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGAc                                                           <  1:1483984/100‑1 (MQ=255)

                                    atatTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGAc                                                           >  2:90366/1‑100 (MQ=255)

                                    atatTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGAc                                                           <  1:362324/100‑1 (MQ=255)

                                     tatTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACa                                                          <  2:2693903/100‑1 (MQ=255)

                                     tatTCCCCGACACCATCAGGGTGAAGGGGATTAAGCCCACATTGTGAGTACCAGCATCCAAAGACATAATTAAACAGCTGCGAAGGGGAGGTTCGAGACa                                                          >  1:1362758/1‑100 (MQ=255)

                                       ttCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAACCTCCTGCGAAGGCGAGCTTCGTGAc                                                           <  1:295179/97‑1 (MQ=255)

                                         ccccGACACCATCAGGGTGACGGGGATTAAGCCCACTTTATGAACATCAGCATCCAAAGACATAACTAAACTCCTGCGAAGGCGAGCTTCATGACAATaa                                                      >  1:1528488/1‑100 (MQ=255)

                                          cccGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATaaa                                                     >  2:185186/1‑100 (MQ=255)

                                          cccGACAACATCAGGGTGACGGGCATTAAGCCCACTTTGTGAGCATCAACATCCAAAGACATAACTACCCTCCTGAGAAGGCGAGCTTAGTGa                                                            >  1:1574835/1‑93 (MQ=255)

                                                acCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGt                                               <  2:2330742/100‑1 (MQ=255)

                                                 ccATCAGGGTGACGGGGCTTACGCCCACTCTGTGAGCACCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACCATAAATTACGtc                                              <  1:2147365/100‑1 (MQ=255)

                                                 ccATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGtc                                              >  1:28096/1‑100 (MQ=255)

                                                 ccATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGtc                                              >  1:1410012/1‑100 (MQ=255)

                                                  cATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGtca                                             >  2:1283128/1‑100 (MQ=255)

                                                    ttccgatctcGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGtcata                                           <  1:977061/91‑1 (MQ=255)

                                                     cAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGtcatat                                          <  1:636727/100‑1 (MQ=255)

                                                     cAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGtcatat                                          <  1:863943/100‑1 (MQ=255)

                                                     cAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGtcata                                           >  2:278988/1‑99 (MQ=255)

                                                      aGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGtcatatc                                         <  2:516823/100‑1 (MQ=255)

                                                       tggTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGtcatatca                                        <  1:2864059/99‑1 (MQ=255)

                                                       gggTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGtcatatca                                        >  2:2285877/1‑100 (MQ=255)

                                                        ggTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGtcatatcaa                                       >  2:2864078/1‑99 (MQ=255)

                                                           gACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACg                                    >  1:225002/1‑100 (MQ=255)

                                                             cGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGcc                                  >  2:2199272/1‑100 (MQ=255)

                                                              ggggATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCt                                 >  2:775107/1‑100 (MQ=255)

                                                               gggATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTg                                >  2:540162/1‑100 (MQ=255)

                                                               gggATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCAAACGCCTg                                >  2:2916174/1‑100 (MQ=255)

                                                                ggATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGc                               <  1:1276871/100‑1 (MQ=255)

                                                                ggATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGc                               <  1:786480/100‑1 (MQ=255)

                                                                ggATTAAGCCCACTTTGTGAGCATCAGCATCAATAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTg                                <  1:596292/99‑1 (MQ=255)

                                                                 gATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGCCATAAAATACGcc                                  >  1:749729/1‑96 (MQ=255)

                                                                     aaGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCttgat                             >  1:1442348/1‑93 (MQ=255)

                                                                      aGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGa                         >  1:2492142/1‑100 (MQ=255)

                                                                      aGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGa                         >  2:473236/1‑100 (MQ=255)

                                                                        cccACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATa                       >  2:2108474/1‑100 (MQ=255)

                                                                         ccACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAg                      >  1:1907527/1‑100 (MQ=255)

                                                                          cACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAg                      >  2:826779/1‑99 (MQ=255)

                                                                          cACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGt                     <  2:1076540/100‑1 (MQ=255)

                                                                          cACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGt                     >  2:2677732/1‑100 (MQ=255)

                                                                            cTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTg                   <  2:387508/100‑1 (MQ=255)

                                                                            cTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTg                   <  2:49155/100‑1 (MQ=255)

                                                                            cTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTg                   <  2:939126/100‑1 (MQ=255)

                                                                             tttGTGAGCATCAGCAACCAAAGACATAATTAAACTCCTGCGAAGGCGCGCTTCATGACAATAAATTACGTCATATCATACGCCGGCATTCGATAg                      <  1:1996896/96‑1 (MQ=255)

                                                                               tgtgAGCATCAGCCTCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAat                <  2:2621348/100‑1 (MQ=255)

                                                                                gtgAGCATCAGCATCCAAAGACATAATTGAACTCCTGCGCAGGCGCGCTTCGTGACAATCAATCACCTCATATCATACCCCTGCATTCTAGAGTTGAat                >  1:1620224/1‑99 (MQ=255)

                                                                                 tgAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTACATTCGATAGTAGAatta              >  1:547697/1‑100 (MQ=255)

                                                                                  gAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAattan             <  2:1430385/100‑2 (MQ=255)

                                                                                   agcatcagcatcCAAAGACATAATTAATCTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAattat             >  1:1041949/1‑99 (MQ=255)

                                                                                   agcatcagcatcCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAattatt            >  2:1728672/1‑100 (MQ=255)

                                                                                    gcatcagcatcCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAAttatta           >  2:253991/1‑100 (MQ=255)

                                                                                    gcatcagcatcCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAAttatta           >  2:1795150/1‑100 (MQ=255)

                                                                                     catcagcatcCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATtattat          <  2:114960/100‑1 (MQ=255)

                                                                                      atcagcatcCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATc         <  1:438431/100‑1 (MQ=255)

                                                                                      atcagcatcCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATc         <  1:2234908/100‑1 (MQ=255)

                                                                                      atcagcatcCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATc         <  2:1951141/100‑1 (MQ=255)

                                                                                       tcagcatcCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCg        <  1:2680667/100‑1 (MQ=255)

                                                                                       tcagcatcCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCg        >  2:1762265/1‑100 (MQ=255)

                                                                                          gcatcCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAattatgtctttgg     >  2:320797/1‑92 (MQ=255)

                                                                                          gcatcCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGAcc     <  2:327415/100‑1 (MQ=255)

                                                                                           catcCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCg    >  2:1620130/1‑100 (MQ=255)

                                                                                            atcCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATAGCATACGCCTGCATTCGATAGTTGAATTATTATCGACCgg   <  1:2466135/100‑1 (MQ=255)

                                                                                             tcCAAAGACATAATTAAACTCCTGCGAGGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCggg  >  1:2532211/1‑100 (MQ=255)

AGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGG  >  NC_000913/4336928‑4337120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A