Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F232 I0 R1 243 156.3 7646460 93.8% 7172379 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 107,553 (T)9→8 100% intergenic (+79/‑152) lpxC → / → secM UDP‑3‑O‑acyl N‑acetylglucosamine deacetylase/regulator of secA translation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913107,5450T.93.2% 522.6 / 31.1 133intergenic (+71/‑160)lpxC/secMUDP‑3‑O‑acyl N‑acetylglucosamine deacetylase/regulator of secA translation
Reads supporting (aligned to +/- strand):  ref base T (7/2);  new base . (50/74);  total (57/76)
Fisher's exact test for biased strand distribution p-value = 3.79e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01

Only 100 of 149 total aligned reads displayed.

AAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCA  >  NC_000913/107448‑107644
                                                                                                 |                                                                                                   
aaaGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCCCTCTCTCCGGCCAGGTGAACCAGTCGttt                                                                                                   >  2:810592/1‑100 (MQ=255)
aaaGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGttt                                                                                                   <  1:3567286/100‑1 (MQ=255)
 aaGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGCACCAGTCGtttt                                                                                                  >  1:1092520/1‑100 (MQ=255)
     gcCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGtttttttt                                                                                              <  2:3534697/100‑1 (MQ=255)
      ccTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGttttttttg                                                                                             <  1:1170931/100‑2 (MQ=255)
      ccTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGttttttttg                                                                                             >  2:847925/1‑99 (MQ=255)
      ccTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGttttttttg                                                                                             >  1:3772692/1‑99 (MQ=255)
      ccTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGttttttttg                                                                                             <  1:653148/100‑2 (MQ=255)
       cTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGttttttttga                                                                                            >  1:3654570/1‑98 (MQ=255)
       cTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGttttttttga                                                                                            >  1:671101/1‑98 (MQ=255)
         tCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGttttttttt                                                                                             >  2:2025535/1‑97 (MQ=255)
           aGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAAttt                                                                                       >  1:1866371/1‑100 (MQ=255)
            gCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTT‑AAtttt                                                                                      >  1:304846/1‑100 (MQ=255)
            gCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAAtttt                                                                                      >  2:140693/1‑100 (MQ=255)
             cTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTta                                                                                     <  1:2402343/100‑1 (MQ=255)
             cTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTta                                                                                     <  1:430186/100‑1 (MQ=255)
             cTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTta                                                                                     <  2:171901/100‑1 (MQ=255)
             cTGTACGGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCTCTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAAttttn                                                                                     <  2:3397474/100‑2 (MQ=255)
               gTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTT‑TATTTtata                                                                                   >  2:3245490/1‑100 (MQ=255)
               gTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTtata                                                                                   <  2:2093807/100‑1 (MQ=255)
               gTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTta                                                                                     >  2:3172999/1‑98 (MQ=255)
                 aCTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAag                                                                                 <  2:3794563/100‑1 (MQ=255)
                 aCTGGCATAACGACATTTATACTGTCGGATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGTCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAag                                                                                 <  2:340723/100‑1 (MQ=255)
                  cTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTT‑AATTTTATAaga                                                                                >  1:1712084/1‑100 (MQ=255)
                    ggCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAagaga                                                                              <  2:1227441/100‑2 (MQ=255)
                    ggCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCAATCTCTCCGGCCAGGTGAACCAGTCG‑TCTTTTTTGCATTTTATAag                                                                                 >  2:1156830/1‑97 (MQ=255)
                            gACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATaaaaa                                                                      >  1:486278/1‑100 (MQ=255)
                              cATTTATACCGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTCAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATaaaaaa                                                                     >  2:2925549/1‑99 (MQ=255)
                               aTTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATaaaaaa                                                                     >  2:3557739/1‑98 (MQ=255)
                                tttATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACgg                                                                  <  1:3501150/100‑1 (MQ=255)
                                  tataCTGTCGTATAAAATTCGCCTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATCACAAACgct                                                                 >  2:3026663/1‑97 (MQ=255)
                                  tataCTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGATCCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGt                                                                 <  1:138627/99‑1 (MQ=255)
                                  tataCTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTg                                                                >  2:3495089/1‑100 (MQ=255)
                                    taCTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCg                                                              <  2:1776788/100‑1 (MQ=255)
                                    taCTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCg                                                              <  1:2681694/100‑1 (MQ=255)
                                      cTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGaa                                                            >  1:1762093/1‑100 (MQ=255)
                                       tGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAAc                                                           <  1:309603/100‑1 (MQ=255)
                                           gTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTTCGAACGCt                                                        >  2:701286/1‑99 (MQ=255)
                                            tataAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGt                                                      >  1:2748481/1‑100 (MQ=255)
                                             ataAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATCAGAGCTATAAAAAACGGTGCGAACGCTGtt                                                     >  2:1567760/1‑100 (MQ=255)
                                             ataAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGtt                                                     >  1:3001729/1‑100 (MQ=255)
                                             ataAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGtt                                                     >  1:119003/1‑100 (MQ=255)
                                              taAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGttt                                                    <  1:864990/100‑1 (MQ=255)
                                               aaaaTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGtttt                                                   <  1:1382252/100‑1 (MQ=255)
                                                  aTTCGACTGGCAAATCTGGCACTCTCTCCGGACAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCtt                                                <  2:920210/100‑1 (MQ=255)
                                                   ttCTACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTa                                               <  1:3236091/100‑1 (MQ=255)
                                                   ttCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTa                                               <  1:1473056/100‑1 (MQ=255)
                                                    tCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTaa                                              <  2:18669/100‑1 (MQ=255)
                                                     cGACTGGCAAATGTGGCACTCTCTCCGGCCAGGTGAACCAGTAG‑TTTTTTTTGTATTTTATAAAAGATCTAAATAACGGTGGCAACGGTGTTTTCGTa                                               >  2:3330704/1‑98 (MQ=255)
                                                     cGACTGGCAAATCTGTCATTCTCTCTGGCCAGGTGAACAAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAg                                             <  2:1890046/100‑1 (MQ=255)
                                                     cGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTaa                                              <  1:2402649/99‑1 (MQ=255)
                                                     cGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAg                                             >  2:1194640/1‑100 (MQ=255)
                                                      gACTGGCAAATCTGGCATTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAg                                             <  2:2203322/99‑1 (MQ=255)
                                                      gACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGc                                            <  1:2491907/100‑1 (MQ=255)
                                                      gACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAAAAAAATAAAAAACGGTGCGAACGCTGTTTTCTTAAGc                                            <  2:3820748/100‑1 (MQ=255)
                                                        cTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCAc                                          <  1:349131/100‑1 (MQ=255)
                                                        cTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCAc                                          >  1:2537385/1‑100 (MQ=255)
                                                         tGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACt                                         >  1:2243326/1‑100 (MQ=255)
                                                          ggCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACtt                                        >  1:1738047/1‑100 (MQ=255)
                                                          ggCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACtt                                        >  1:1737507/1‑100 (MQ=255)
                                                          ggCAAATCTGGCACTCTCTCAGGCCAGGTGAACCAGTCGTTTTTTTTT‑AATTTTATAAGAGGTATAAAAAACGATACGAACGCTGTTTTCTTAAGCACtt                                        >  2:662273/1‑100 (MQ=255)
                                                            cAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTGAGCAAtttt                                      >  2:764870/1‑100 (MQ=255)
                                                            cAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACtttt                                      <  2:1543262/100‑1 (MQ=255)
                                                            cAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACtttt                                      >  1:3274933/1‑100 (MQ=255)
                                                             aaaTCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCa                                           >  2:292916/1‑94 (MQ=255)
                                                             aaaTCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTc                                     >  1:642789/1‑100 (MQ=255)
                                                             aaaTCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTc                                     >  1:2707177/1‑100 (MQ=255)
                                                              aaTCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTcc                                    <  1:2825893/100‑1 (MQ=255)
                                                              aaTCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTcc                                    <  2:1155115/100‑1 (MQ=255)
                                                               aTCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTT‑TTTTTTTTTAGAGTCAAAAAAAACCGTGACCACGCTTTTTTCCTTGGCAAtttt                                      >  2:1727878/1‑97 (MQ=255)
                                                               aTCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCg                                   <  1:769016/100‑1 (MQ=255)
                                                                tCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGc                                  <  1:2024129/100‑1 (MQ=255)
                                                                tCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGc                                  <  1:2287919/100‑1 (MQ=255)
                                                                tCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGc                                  <  1:2413265/100‑1 (MQ=255)
                                                                tCTGGCACTCCCCCCGGCAAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCg                                   <  1:717541/99‑1 (MQ=255)
                                                                 cTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGca                                 <  1:1336062/100‑1 (MQ=255)
                                                                  tGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGcac                                <  1:850902/100‑1 (MQ=255)
                                                                   ggCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTT‑TTTTTTTTAAGAGCTATAAAAAACGGTGCGAACGCCGTTTTCTTAAGCCATTTTCCg                                   >  2:1049525/1‑96 (MQ=255)
                                                                   ggCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGcaca                               <  2:491416/100‑1 (MQ=255)
                                                                   ggCACGCTCTCCGGCATGGTGAACCAGTCG‑TTTTTTGTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGc                                  <  2:1355619/97‑1 (MQ=255)
                                                                    gCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACaa                              >  2:1905230/1‑100 (MQ=255)
                                                                     cattctctcCGGCCAAGTGAACCAGTCGTTTTTTTTT‑AATTTTATAAAACCTATAAAACACGGTGCGAACGATGTTTTCTTAAGCACTTTTCCGCACAAc                             <  2:2397043/97‑1 (MQ=255)
                                                                      aCTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACt                            <  1:714823/100‑1 (MQ=255)
                                                                      aCTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACt                            <  2:1644439/100‑1 (MQ=255)
                                                                       ctctctCGGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACtt                           >  1:1994186/1‑100 (MQ=255)
                                                                       ctctctCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACt                            <  1:142038/99‑1 (MQ=255)
                                                                         ctctcCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACACTGTTTTCTTAAGCACTTTTCCGCACAAc                             >  1:3221110/1‑96 (MQ=255)
                                                                           ctcCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCt                       >  1:983477/1‑100 (MQ=255)
                                                                            tcCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCtt                      <  1:1331039/100‑1 (MQ=255)
                                                                               ggCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCAt                   >  1:2864911/1‑100 (MQ=255)
                                                                               ggCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCAt                   <  1:2868771/100‑1 (MQ=255)
                                                                                gCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCAtt                  <  2:1614782/100‑1 (MQ=255)
                                                                                  cAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCg                <  1:2657035/100‑1 (MQ=255)
                                                                                     gTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGc             <  1:60875/100‑1 (MQ=255)
                                                                                        aaCCCGTCG‑TTTTTTTTGTATTATATGAGAGATATAAACACCGCTGCGAACGCTGTTTTGTACACCCCCTTCCCGGCCAACTTACCTTCATTCGt               >  2:2354785/1‑95 (MQ=255)
                                                                                         aCCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCtgtg         <  2:3610561/100‑1 (MQ=255)
                                                                                         aCCAGTCG‑TGTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCtgtg         <  2:501735/100‑1 (MQ=255)
                                                                                          ccAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTgg        <  1:2163333/100‑1 (MQ=255)
                                                                                            aGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGATGCGACTGCTGTTTACTTAAGCACTTTTCCGCCCAACTGATCTTCATTCGTGCGATGGa       >  2:2633986/1‑99 (MQ=255)
                                                                                                 tttttttttGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCa  <  1:2990579/100‑1 (MQ=255)
                                                                                                 |                                                                                                   
AAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCA  >  NC_000913/107448‑107644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A