Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F125 I0 R1 65 108.6 5319566 96.1% 5112102 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 107,553 (T)9→8 100% intergenic (+79/‑152) lpxC → / → secM UDP‑3‑O‑acyl N‑acetylglucosamine deacetylase/regulator of secA translation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913107,5450T.93.9% 406.1 / 20.1 99intergenic (+71/‑160)lpxC/secMUDP‑3‑O‑acyl N‑acetylglucosamine deacetylase/regulator of secA translation
Reads supporting (aligned to +/- strand):  ref base T (6/0);  new base . (37/56);  total (43/56)
Fisher's exact test for biased strand distribution p-value = 5.44e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 116 total aligned reads displayed.

TCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGAC  >  NC_000913/107446‑107640
                                                                                                   |                                                                                               
tCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGt                                                                                                 >  1:2290189/1‑100 (MQ=255)
  aaaGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGttt                                                                                               >  2:1539422/1‑100 (MQ=255)
      cgcCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGttttttt                                                                                           <  1:2501436/100‑1 (MQ=255)
      cgcCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGttttttt                                                                                           <  1:2000908/100‑1 (MQ=255)
       gcCTTCAGCTGTACTGGCATAACTACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGtttttttt                                                                                          <  1:651205/100‑1 (MQ=255)
       gcCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGtttttttt                                                                                          <  2:1353423/100‑1 (MQ=255)
        ccTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGttttttttg                                                                                         >  1:449135/1‑99 (MQ=255)
        ccTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGttttttttg                                                                                         >  1:1461181/1‑99 (MQ=255)
        ccTTCAGCTGTACTGGCATAACGACATTTAAACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGttttttttt                                                                                         <  2:1358303/100‑1 (MQ=255)
         ctacatctttACTGTCATAACGACTTTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGtttttttt                                                                                          <  1:2482352/89‑1 (MQ=255)
         cTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGttttttttga                                                                                        >  1:153487/1‑98 (MQ=255)
           tCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGttttttttgaa                                                                                       <  2:1728894/99‑4 (MQ=255)
           tCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACAAGACGttttttttgaat                                                                                      >  1:2388251/1‑96 (MQ=255)
             aGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGTACTCTGTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAAtt                                                                                    <  2:593687/99‑1 (MQ=255)
              gCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGttttttttgcatttt                                                                                   >  1:26359/1‑93 (MQ=255)
              gCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAAtttt                                                                                  <  1:1336318/100‑1 (MQ=255)
              gCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCAACTGGCAAAGCTGGCACTCACTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAAtttt                                                                                  <  1:1643067/100‑1 (MQ=255)
               cTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTta                                                                                 <  1:1713917/100‑1 (MQ=255)
                tGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTtat                                                                                <  1:862742/100‑1 (MQ=255)
                 gTACTTGCATATCGACATTTATACTGTCGTATAATTTTCGACTGGCAAATCTGGCACGCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTtata                                                                               <  1:711638/100‑1 (MQ=255)
                 gTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGCATTTtat                                                                                >  2:250983/1‑99 (MQ=255)
                 gTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTtata                                                                               >  2:2351449/1‑100 (MQ=255)
                 gTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGACAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTtata                                                                               <  2:1193277/100‑1 (MQ=255)
                  tACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATaa                                                                              <  1:1585301/100‑1 (MQ=255)
                    cTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAaga                                                                            >  2:2046679/1‑100 (MQ=255)
                    cTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAaga                                                                            >  2:1583463/1‑100 (MQ=255)
                    cTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTAG‑TTTTTTTTGAATTTTATAaga                                                                            >  1:2309367/1‑100 (MQ=255)
                    cTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCAGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAaga                                                                            >  1:2254167/1‑100 (MQ=255)
                      ggCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGc                                                                          >  2:1327344/1‑100 (MQ=255)
                      ggCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGc                                                                          <  2:196495/100‑1 (MQ=255)
                        cATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTT‑ATTTTTTTAAGAGCta                                                                        >  2:837133/1‑100 (MQ=255)
                        cATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCta                                                                        <  2:2009760/100‑1 (MQ=255)
                        cATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCta                                                                        <  1:1121763/100‑1 (MQ=255)
                        cATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCta                                                                        <  1:1121740/100‑1 (MQ=255)
                         aTAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCtat                                                                       >  1:1956766/1‑100 (MQ=255)
                          tAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCtata                                                                      <  2:686482/100‑1 (MQ=255)
                           aaCGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATaa                                                                     >  2:2044293/1‑100 (MQ=255)
                            aCGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATaaa                                                                    >  2:234984/1‑100 (MQ=255)
                            aCGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATaaa                                                                    >  1:2562073/1‑100 (MQ=255)
                                cATTTATACTTTCGTATAAAATTTGACTGTCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATaaaaaa                                                                 <  2:856917/99‑1 (MQ=255)
                                cATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAAc                                                                <  1:1943886/100‑1 (MQ=255)
                                  tttATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACgg                                                              <  2:1988426/100‑1 (MQ=255)
                                    tataCTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTg                                                            >  1:2280476/1‑100 (MQ=255)
                                     ataCTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGc                                                           >  1:2468508/1‑100 (MQ=255)
                                      taCTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGc                                                           >  1:2153632/1‑100 (MQ=255)
                                       aCTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGa                                                         <  1:1761563/100‑1 (MQ=255)
                                        cTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGaa                                                        >  2:1341450/1‑100 (MQ=255)
                                          gTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACg                                                      >  2:2547835/1‑100 (MQ=255)
                                          gTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACg                                                      <  1:988506/100‑1 (MQ=255)
                                            cGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGc                                                     >  2:2359242/1‑99 (MQ=255)
                                            cGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCt                                                    >  1:1415785/1‑100 (MQ=255)
                                            cGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCt                                                    >  1:2426358/1‑100 (MQ=255)
                                            cGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCt                                                    >  1:1206980/1‑100 (MQ=255)
                                              tataAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGt                                                  >  2:676244/1‑100 (MQ=255)
                                               ataAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTT‑AATTTTTTAAGAGCTATAAAAAAAGGTGCCAACGCtttt                                                 >  2:1654129/1‑100 (MQ=255)
                                               ataAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGtt                                                 >  1:782108/1‑100 (MQ=255)
                                                naaaaTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAACTATAAAAAACGGTGCGAACGCTGttt                                                >  2:759271/2‑100 (MQ=255)
                                                  aaaTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGTGAACGCTGtttgc                                              <  1:1800234/100‑3 (MQ=255)
                                                  aaaTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTc                                              <  2:399544/100‑1 (MQ=255)
                                                   aaTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCt                                             <  2:638511/100‑1 (MQ=255)
                                                    aTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCtt                                            >  1:5410/1‑100 (MQ=255)
                                                     ttCGACTGGCAAATCTTGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTa                                           <  1:1167648/100‑1 (MQ=255)
                                                     ttCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTa                                           <  1:43564/100‑1 (MQ=255)
                                                      tCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTaa                                          <  2:140931/100‑1 (MQ=255)
                                                       cGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTaan                                         <  2:854649/100‑2 (MQ=255)
                                                       cGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAg                                         <  2:2427035/100‑1 (MQ=255)
                                                       cGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAg                                         <  2:332884/100‑1 (MQ=255)
                                                          cTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCAc                                      >  2:583531/1‑100 (MQ=255)
                                                          cTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCAc                                      >  1:474201/1‑100 (MQ=255)
                                                          cTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCAc                                      <  1:167342/100‑1 (MQ=255)
                                                           tGGCAAATCTTTCACGCTCGCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACt                                     <  1:1542568/100‑1 (MQ=255)
                                                            ggCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAATAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACtt                                    >  2:285627/1‑100 (MQ=255)
                                                            ggCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACtt                                    >  1:1421468/1‑100 (MQ=255)
                                                             gCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACttt                                   >  1:683406/1‑100 (MQ=255)
                                                             gCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACttt                                   <  2:2309339/100‑1 (MQ=255)
                                                              cAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACtttt                                  >  2:863986/1‑100 (MQ=255)
                                                                aaTTTGGCCCTTTCTCCCGCCAGGGGACCCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTcc                                <  2:1993908/100‑1 (MQ=255)
                                                                  tCTTGCACTCTCTCCTGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGc                              <  1:612489/100‑1 (MQ=255)
                                                                   cTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGca                             <  1:2391669/100‑1 (MQ=255)
                                                                       cACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAAc                         <  1:366500/100‑1 (MQ=255)
                                                                       cACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAAc                         <  1:1327357/100‑1 (MQ=255)
                                                                        aCTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACt                        <  1:2046701/100‑1 (MQ=255)
                                                                         ctctctCCGGCCAGGTGAACCAGTCGTTTTTTTTT‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACtt                       >  2:255396/1‑100 (MQ=255)
                                                                          tctctcCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTa                      <  1:2182596/100‑1 (MQ=255)
                                                                           ctctcCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTAt                     <  2:1155597/100‑1 (MQ=255)
                                                                            tctcCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTAt                     >  1:640703/1‑99 (MQ=255)
                                                                             ctcCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATc                    <  2:1038984/99‑1 (MQ=255)
                                                                             ctcCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCt                   <  2:2362215/100‑1 (MQ=255)
                                                                               ccGGCCAGGTGAACCAGTCGTTTTTTTTT‑TTTTTTTTAAAAGATAAAAAGAACGGTGGGAGCGTTGATTGCCTAAGGAATTTTTCGctcgtctt                       >  2:966269/1‑87 (MQ=255)
                                                                               ccGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAACACGGTGCAAACGCTGTTTTCTTAAGCACTTTTCCGCACAAATTATCTTc                 >  2:2404890/1‑100 (MQ=255)
                                                                                  gCCAGGTGATCCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCAtt              <  1:1368271/100‑1 (MQ=255)
                                                                                  gCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAATGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCAtt              <  1:357964/100‑1 (MQ=255)
                                                                                   ccAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTc             <  2:153486/100‑1 (MQ=255)
                                                                                    cAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCg            <  2:1780620/100‑1 (MQ=255)
                                                                                      ggTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCg            >  2:683094/1‑98 (MQ=255)
                                                                                       gTGATCCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGc         <  1:940992/100‑1 (MQ=255)
                                                                                       gTGAACCAGTCG‑TTTCTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGc         <  1:2614489/100‑1 (MQ=255)
                                                                                         gAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTCAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCtg       <  2:1411073/100‑1 (MQ=255)
                                                                                            ccAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCtgtg     <  1:908740/99‑1 (MQ=255)
                                                                                              aGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGAc  <  1:2044315/100‑1 (MQ=255)
                                                                                                   |                                                                                               
TCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGAC  >  NC_000913/107446‑107640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A