Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F232 I0 R1 243 156.3 7646460 93.8% 7172379 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,202,686 C→T 16.3% L266F (CTC→TTC)  cca → fused tRNA nucleotidyl transferase/2'3'‑cyclic phosphodiesterase/2'nucleotidase and phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,202,6860CT16.3% 460.9 / 69.7 195L266F (CTC→TTC) ccafused tRNA nucleotidyl transferase/2'3'‑cyclic phosphodiesterase/2'nucleotidase and phosphatase
Reads supporting (aligned to +/- strand):  ref base C (88/75);  new base T (16/16);  total (104/91)
Fisher's exact test for biased strand distribution p-value = 7.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.55e-01

Only 100 of 204 total aligned reads displayed.

TTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGCCA  >  NC_000913/3202587‑3202785
                                                                                                   |                                                                                                   
ttAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAAc                                                                                                     >  2:3607121/1‑100 (MQ=255)
  aaTGTCGCTCCCAGTGGTGGCTATGCTGAGTCCTCACTTCGAGATCCGTTTCGCAACGTTATGCCTCGATCTCGGTAAAGGGCTGACGCCGCCAGAActc                                                                                                   <  2:1064294/100‑1 (MQ=255)
    tGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTg                                                                                                 >  2:625282/1‑100 (MQ=255)
    tGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTg                                                                                                 >  2:3138770/1‑100 (MQ=255)
       cGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGcc                                                                                              >  2:1355386/1‑100 (MQ=255)
       cGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGcc                                                                                              >  1:1284902/1‑100 (MQ=255)
       cGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGcc                                                                                              >  2:3362757/1‑100 (MQ=255)
       cGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGcc                                                                                              >  2:3473797/1‑100 (MQ=255)
        gCTCTCAATGGCAGCGATGCTGAGTCCGCAGGTCGATGTCCGTCTCGCAACTTGATGCCACGAGCTCGGTAAAGGGCTGACGCCGCCAGAACTCTgg                                                                                                <  2:1012762/97‑1 (MQ=255)
         ctctCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCcgc                                                                                            >  1:821863/1‑100 (MQ=255)
         ctctCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCcgc                                                                                            >  2:1580875/1‑100 (MQ=255)
          tctcAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCcgcg                                                                                           <  2:1745415/100‑1 (MQ=255)
          tctcAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCcgcg                                                                                           <  1:3640417/100‑1 (MQ=255)
            tcAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGtc                                                                                         >  1:1441140/1‑100 (MQ=255)
            tcAATGGCGCCGATACTGCGTCCGGAGGTCGAGGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCGGCCAGAGTTCTGGCGGCGtc                                                                                         <  2:3428819/100‑1 (MQ=25)
             cAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCGGCCAGAGTTCTGGCGGCGtc                                                                                         <  1:2675173/99‑1 (MQ=38)
             cAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGtca                                                                                        >  1:3328028/1‑100 (MQ=255)
               aTGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCGGCCAGAGTTCTGGCGGCGtcagc                                                                                      <  1:430282/100‑3 (MQ=38)
               aTGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGtcatc                                                                                      >  2:1761381/1‑100 (MQ=255)
               aTGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGtcatc                                                                                      >  1:394707/1‑100 (MQ=255)
               aTGGCGGCGATGCGGAGTCCGCAGGTGGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCGGCCAGAGTTCTGGCGGCGtcagc                                                                                      <  2:570116/100‑3 (MQ=37)
                tGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCGGCCAGAGTTCTGGCGGCGtcagcc                                                                                     <  1:689149/100‑4 (MQ=37)
                tGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGtcatca                                                                                     <  1:581084/100‑1 (MQ=255)
                 ggcggcGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCGGCCAGAGTTCTGGCGGCGtcagccc                                                                                    <  1:3695214/100‑5 (MQ=37)
                 ggcggcGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTcatcat                                                                                    >  2:1283618/1‑100 (MQ=255)
                   cggcgATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCCGATGCCGCCAGAACTCTGGCCGCGTCAtcatgg                                                                                  <  2:2508367/100‑1 (MQ=255)
                    ggcgATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCAtcatggt                                                                                 <  1:1288227/100‑1 (MQ=255)
                      cgATGCTGAGTCCTCAGGTCGATGTCCTTTTCGCCACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCAtcatggt                                                                                 <  2:1868714/98‑1 (MQ=255)
                      cgATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCAtcatggtc                                                                                >  2:1693389/1‑99 (MQ=255)
                          gCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGc                                                                           >  2:3400422/1‑100 (MQ=255)
                          gCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGc                                                                           >  2:3357982/1‑100 (MQ=255)
                          gCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGc                                                                           <  1:2870864/100‑1 (MQ=255)
                           cTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGcc                                                                          >  2:3631025/1‑100 (MQ=255)
                            tGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGcc                                                                          >  2:1181212/1‑99 (MQ=255)
                               gTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCcggc                                                                      >  1:483690/1‑100 (MQ=255)
                                  cGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCggg                                                                   <  1:1414454/100‑1 (MQ=255)
                                  cGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCggg                                                                   >  1:1071470/1‑100 (MQ=255)
                                  cGCAGGTCGATGGCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCggg                                                                   <  2:3724764/100‑1 (MQ=255)
                                    cAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGgtg                                                                 <  1:1641462/100‑1 (MQ=255)
                                     aGGTCGATGCCCGTATCGCAAATTTATAAACCGATCTCGGTAAAGCGCTGAAGACGACAGAACTCTGGCAGAATCATCATGGTCATGGCCCGGAGGgtg                                                                 <  2:1128433/99‑1 (MQ=255)
                                      ggTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGtt                                                               <  1:2758650/100‑1 (MQ=255)
                                      ggTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGtt                                                               >  2:306492/1‑100 (MQ=255)
                                         cGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAg                                                            >  1:920259/1‑100 (MQ=255)
                                             gTCTGTTTCGCAACTTTATGCCACGATCTGGGTAAAGCGCTTACGCCGCCAGAACTCTGGTCGCGTGAGAATGGTCATGGCCCGGCGTGTGTTAAGTTAg                                                        <  2:2764463/100‑1 (MQ=255)
                                             gTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAg                                                        >  2:3052418/1‑100 (MQ=255)
                                               ccGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTg                                                      <  1:218339/100‑1 (MQ=255)
                                                 gTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGa                                                    <  1:3419244/100‑1 (MQ=255)
                                                   ttCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGaac                                                  <  1:981675/100‑1 (MQ=255)
                                                   ttCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGGTAAGTTAGTGGaac                                                  >  2:3651308/1‑100 (MQ=255)
                                                    tCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCCTGGCCCGGCGGGGGTTAAGTTAGTGGaaca                                                 >  2:2063420/1‑100 (MQ=255)
                                                     cGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCACGGCGGGTGTTAAGTTAGTGGaacaa                                                >  1:2299016/1‑100 (MQ=255)
                                                       cAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACt                                              >  2:2601166/1‑100 (MQ=255)
                                                       cAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACt                                              >  1:1891932/1‑100 (MQ=255)
                                                       cAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACt                                              >  1:2043183/1‑100 (MQ=255)
                                                            ttATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGcc                                         <  1:1026421/100‑1 (MQ=255)
                                                              aTGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAg                                       <  1:305996/100‑1 (MQ=255)
                                                              aTGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAg                                       <  1:2482483/100‑1 (MQ=255)
                                                                gCCACGGTCTCGGTAAAGGGCTGACGCCGCCAGAACTCTTGCCGCGGCATCATGGTCATGGCAAGCCGGGAGCTAAGTTAGAGGAACACCTATGCCa                                        >  2:712679/1‑97 (MQ=255)
                                                                gCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCg                                     >  1:2072723/1‑100 (MQ=255)
                                                                 ccACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGt                                    >  2:3321367/1‑100 (MQ=255)
                                                                  cACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTc                                   <  1:2550279/100‑1 (MQ=255)
                                                                   aCGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCt                                  <  1:563248/100‑1 (MQ=255)
                                                                     gATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTgc                                >  1:2935427/1‑100 (MQ=255)
                                                                     gATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTgc                                >  1:1139632/1‑100 (MQ=255)
                                                                       tctcGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGATCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTCTGCCAGCGTCTgcgc                              >  2:1822387/1‑100 (MQ=255)
                                                                        tctggTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGcgcg                             <  2:2151943/97‑1 (MQ=255)
                                                                        ctcGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTgcgc                              >  2:2494254/1‑99 (MQ=255)
                                                                        ctcGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGcgcg                             >  1:3795898/1‑100 (MQ=255)
                                                                          cGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTg                           <  1:3166400/100‑1 (MQ=255)
                                                                          cGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTg                           >  1:3270702/1‑100 (MQ=255)
                                                                          cGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTg                           <  1:1552580/100‑1 (MQ=255)
                                                                          cGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTg                           <  1:3530786/100‑1 (MQ=255)
                                                                           ggTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGcgcgaga                          >  1:2151957/1‑97 (MQ=255)
                                                                             tAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCa                        <  1:1761391/100‑1 (MQ=255)
                                                                              aaaGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCaa                       <  1:1184712/100‑1 (MQ=255)
                                                                               aaGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCaaa                      >  2:1987585/1‑100 (MQ=255)
                                                                                catgatGACGCGGCCAGAGTTCTGGCGGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCaaa                      >  2:817791/6‑99 (MQ=37)
                                                                                catgatGACGCGGCCAGAGTTCTGGCGGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCATCTGCGCGTGCCaaa                      >  1:20771/6‑99 (MQ=25)
                                                                                aGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAAt                     >  1:1245772/1‑100 (MQ=255)
                                                                                 gggCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATg                    <  1:3473824/100‑1 (MQ=255)
                                                                                   gCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGaa                  >  1:1352012/1‑100 (MQ=255)
                                                                                    cTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGaaa                 <  1:869047/100‑1 (MQ=255)
                                                                                    atGACGCGGCCAGAGTTCTGGCGGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTACCAAATGaaa                 >  1:3809909/2‑100 (MQ=37)
                                                                                     tGACGCGGCCAGAGTTCTGGCGGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAAt                >  2:2303513/1‑100 (MQ=38)
                                                                                      gACGCGGCCAGAGTTCTGGCGGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAAtt               >  1:3370613/1‑100 (MQ=38)
                                                                                        cgcggcCAGAGTTCTGGCGGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTcg             >  2:3210158/1‑100 (MQ=255)
                                                                                        cgcggcCAGAGTTCTGGCGGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTcg             >  1:1578635/1‑100 (MQ=255)
                                                                                        cgcggcCAGAGTTCTGGCGGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTcg             >  2:2190069/1‑100 (MQ=255)
                                                                                        cgcggcCAGAGTTCTGGCGGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTcg             >  2:2128198/1‑100 (MQ=255)
                                                                                           cgccAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGa          <  1:1824797/100‑1 (MQ=255)
                                                                                           cgccAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGa          >  1:725441/1‑100 (MQ=255)
                                                                                           cgccAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGa          <  1:312765/100‑1 (MQ=255)
                                                                                             ccAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGAtt        >  2:2796020/1‑100 (MQ=255)
                                                                                             ccAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGAtt        <  2:539828/100‑1 (MQ=255)
                                                                                              cAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGAttt       <  2:2925602/100‑1 (MQ=255)
                                                                                                 aaCTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGc    <  2:3369819/100‑1 (MQ=255)
                                                                                                 aaCTCTGGCCGCGTCATCATAATCACAGCCCGGCGTCTGTTAAGTTAGTGGAACAGCTATGTCAGCGCCTGTGCGTGTCAAATTAAATTCGCGATTTTGc    <  2:877995/100‑1 (MQ=255)
                                                                                                 aaCTCCGCCCGCGTCATCATGGTCATAGCCCGTCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTa      <  2:1787117/98‑1 (MQ=255)
                                                                                                   ctctGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGCCa  >  2:408977/1‑100 (MQ=255)
                                                                                                   ctctGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGCCa  >  1:1953772/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
TTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGCCA  >  NC_000913/3202587‑3202785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A