Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A16 F201 I0 R1 306 176.9 8831342 93.4% 8248473 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,202,686 C→T 16.4% L266F (CTC→TTC)  cca → fused tRNA nucleotidyl transferase/2'3'‑cyclic phosphodiesterase/2'nucleotidase and phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,202,6860CT16.4% 468.6 / 72.2 200L266F (CTC→TTC) ccafused tRNA nucleotidyl transferase/2'3'‑cyclic phosphodiesterase/2'nucleotidase and phosphatase
Reads supporting (aligned to +/- strand):  ref base C (110/57);  new base T (12/21);  total (122/78)
Fisher's exact test for biased strand distribution p-value = 2.91e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.43e-01

Only 100 of 203 total aligned reads displayed.

AATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGCCA  >  NC_000913/3202589‑3202785
                                                                                                 |                                                                                                   
   gACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAActct                                                                                                  >  1:418227/1‑98 (MQ=255)
     ctctctCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGcc                                                                                              <  1:73409/98‑1 (MQ=255)
     cGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGcc                                                                                              >  2:1939921/1‑100 (MQ=255)
      gCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCAATGTCCGTTTCGCCACTTTATGCCACGATCCCGGGAAAGGGCTGACGCCGCCAGAACTCTggac                                                                                              >  1:3576743/1‑97 (MQ=255)
       ctctCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCcg                                                                                             >  1:3899639/1‑99 (MQ=255)
         ctcAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCcgcg                                                                                           >  2:2629369/1‑99 (MQ=255)
           cAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCGGCCAGAGTTCTGGCGGCGtca                                                                                        <  2:4059247/100‑1 (MQ=38)
           cAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCGGCCAGAGTTCTGGCGGCGtca                                                                                        <  2:2131441/100‑1 (MQ=38)
             aTGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGGCGCGGCCAGAGTTCTGGCGGCGtcagc                                                                                      <  2:3177969/100‑3 (MQ=37)
             aTGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCGGCCAGAGTTCTGGCGGCGtcagc                                                                                      <  2:1678932/100‑3 (MQ=38)
             aTGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCGGCCAGAGTTCTGGCGGCGtcagc                                                                                      <  2:1526844/100‑3 (MQ=38)
             aTGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCGGCCAGAGTTCTGGCGGCGtcagc                                                                                      <  2:656007/100‑3 (MQ=38)
             aTGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCGGCCAGAGTTCTGGCGGCGtcagc                                                                                      <  2:911888/100‑3 (MQ=38)
             aTGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGtcatc                                                                                      >  2:1117502/1‑100 (MQ=255)
             aTGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGtcatc                                                                                      >  2:197693/1‑100 (MQ=255)
              tGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCGGCCAGAGTTCTGGCGGCGtcagcc                                                                                     <  2:2359192/100‑4 (MQ=37)
              tGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGtcatca                                                                                     >  2:1002662/1‑100 (MQ=255)
              tGGCGGCAATGCTGAGTCCGCAGGTCTATGCCTGTCTCGCAGCTTTATGCCACGATCTCGGTAAAGGGCTGACGCGGCCAGGGTTCTGGCGGCGtcagc                                                                                      <  1:211914/99‑3 (MQ=19)
                gggggcaTGCGGAGTCCGCTGGTGGATGTCCGTTTCGCAGCTCGATGCCACGGGCTCGGTAGAGGGCTGACGCCGCCAGAACTCTGGCCGCGtcatca                                                                                     <  1:3708460/92‑1 (MQ=255)
                gcggcgATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCAtcatg                                                                                   >  1:4255119/1‑100 (MQ=255)
                  ggcgATGCTGAGTCCGCAGGGCGATGTCGGTTTCGCAAGTTTATGCCACGATCTCGGGAAAGGGCTGACGCGGCCAGAGTTCTGGCGGCGtcagccct                                                                                   <  1:997688/98‑6 (MQ=21)
                   gcgATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCAtcatggtc                                                                                >  1:1589453/1‑100 (MQ=255)
                   gcgATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCAtcatggtc                                                                                >  1:1507130/1‑100 (MQ=255)
                   gcgATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCAtcatggtc                                                                                <  2:2881021/100‑1 (MQ=255)
                    cgATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCAtcatggtca                                                                               >  1:336270/1‑100 (MQ=255)
                    cgATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCAtcatggtca                                                                               >  1:3443839/1‑100 (MQ=255)
                       tGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCAtcatggtcatgg                                                                            >  2:2032721/1‑100 (MQ=255)
                         cTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGcc                                                                          >  2:1370549/1‑100 (MQ=255)
                          tGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTTAAGGGCTGCCGCCGCCAGAACTCTGGCCGCGTCATCATAGTCATGGc                                                                           >  1:2550946/1‑98 (MQ=255)
                          tGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGccc                                                                         >  1:2545628/1‑100 (MQ=255)
                          tGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGccc                                                                         >  2:3204248/1‑100 (MQ=255)
                             gTCCGCAGGTCGATGTCCGTGTCTCAACGTTATGCCACGAGGTCTGTAAAGGGTTGACGCCACCATAACGCTGGCCGCGTCATCATGAGCATGGGCcggc                                                                      >  1:1980614/1‑100 (MQ=255)
                              tCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCcggcg                                                                     <  2:1507136/100‑1 (MQ=255)
                              tCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCcggcg                                                                     <  1:1393497/100‑1 (MQ=255)
                                cGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCcggcgg                                                                    >  1:3345475/1‑99 (MQ=255)
                                cGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCggg                                                                   <  2:2533508/100‑1 (MQ=255)
                                 gtaGGTCTTTGTTCGTTTCTCATCTTTATGCCATGTTTTCTGTAAAGGGCTGACGCCGCCAGTACTCTGGCCGCGTCTTCTTTGTCATGGCTCGGCggg                                                                   <  1:1107992/97‑1 (MQ=255)
                                 gCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGCTCTCGGTAAAAGGCTGACGCTGCCAGAACTCTGGCTGGGTCATCATGGTCATGGCCGCGCggg                                                                   >  1:3032803/1‑99 (MQ=255)
                                 gCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATGTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGgt                                                                  >  1:2350831/1‑100 (MQ=255)
                                 gCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGgt                                                                  <  2:4144/100‑1 (MQ=255)
                                 gCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGgt                                                                  <  2:552450/100‑1 (MQ=255)
                                  cAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGgtg                                                                 <  2:415776/100‑1 (MQ=255)
                                  cAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGgtg                                                                 <  1:3523464/100‑1 (MQ=255)
                                    ggTCGATGTCCGTTTCGCAACTTTATGCCCCGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGtt                                                               <  1:3222160/100‑1 (MQ=255)
                                    ggTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGtt                                                               <  2:1220140/100‑1 (MQ=255)
                                    ggTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGtt                                                               >  1:1704462/1‑100 (MQ=255)
                                    ggTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGtt                                                               >  2:3952457/1‑100 (MQ=255)
                                     ggcaaagTCCGTTCCGCAGCTGTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCggg                                                                   <  1:1720490/89‑1 (MQ=255)
                                      tCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTaa                                                             >  1:3763281/1‑100 (MQ=255)
                                      tCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTaa                                                             <  2:1486807/100‑1 (MQ=255)
                                        gATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGt                                                           >  2:2946844/1‑100 (MQ=255)
                                         aTGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGtt                                                          <  2:3507123/100‑1 (MQ=255)
                                         aTGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGtt                                                          <  2:3900306/100‑1 (MQ=255)
                                            tCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGt                                                       >  2:867865/1‑100 (MQ=255)
                                             ccGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTg                                                      >  1:1619626/1‑100 (MQ=255)
                                             ccGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTg                                                      <  1:930105/100‑1 (MQ=255)
                                                 ttCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGaac                                                  >  1:1890910/1‑100 (MQ=255)
                                                   cGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGaacaa                                                >  1:1019643/1‑100 (MQ=255)
                                                     cAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGaacaac                                               >  2:1771160/1‑99 (MQ=255)
                                                     cAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACt                                              <  2:1376996/100‑1 (MQ=255)
                                                        cTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATg                                           >  2:1258070/1‑100 (MQ=255)
                                                        cTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATg                                           >  1:747668/1‑100 (MQ=255)
                                                          ttATGCCATGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGcc                                         >  1:474207/1‑100 (MQ=255)
                                                             tGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGc                                      >  2:3955029/1‑100 (MQ=255)
                                                               ccACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCAGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGt                                    >  1:2364082/1‑100 (MQ=255)
                                                                cACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTc                                   >  1:698202/1‑100 (MQ=255)
                                                                  cGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTg                                 <  2:281355/100‑1 (MQ=255)
                                                                   gATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTgc                                >  1:490566/1‑100 (MQ=255)
                                                                    aTCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGGGTTAAGTTAGTGGAACAACTATGCCAGCGTCTgc                                >  1:3977813/1‑99 (MQ=255)
                                                                      ctcGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGcgcg                             >  2:3044747/1‑100 (MQ=255)
                                                                      ctcGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGcgcg                             >  2:2586719/1‑100 (MQ=255)
                                                                        cGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCTCGTCATCATGGCCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTg                           <  1:3933137/100‑1 (MQ=255)
                                                                        cGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTg                           >  2:1159444/1‑100 (MQ=255)
                                                                        cGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTg                           <  2:2515672/100‑1 (MQ=255)
                                                                         ggTCAAGGGATGCCGCCCCCAGAACTCTGGCCGCGTCACCCTCGTCATGGCCCGGCGGCTGTTCAGATAGTGGACCAACTATGCCAGCGCCTGCGCGta                           <  1:2629354/99‑2 (MQ=255)
                                                                          gTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGcc                         >  1:1221281/1‑100 (MQ=255)
                                                                           tAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCa                        <  2:1579353/100‑1 (MQ=255)
                                                                              aGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCaaa                      >  2:435401/1‑99 (MQ=255)
                                                                              aGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAAt                     <  2:3436173/100‑1 (MQ=255)
                                                                               gggCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCCAATg                    >  1:856223/1‑100 (MQ=255)
                                                                                 gCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGaa                  <  2:2788372/100‑1 (MQ=255)
                                                                                 gCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGaa                  >  1:2506565/1‑100 (MQ=255)
                                                                                  cTGTCGCCACCAGAACTCTGGCCGCGTCATCATTGTCTTGGCCCGGCTGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTTCCAAATGaaa                 <  1:3315864/100‑1 (MQ=255)
                                                                                   tGACGCGGCCAGAGTTCTGGCGGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAAt                >  1:2252809/1‑100 (MQ=38)
                                                                                   tGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAAt                <  2:1705899/100‑1 (MQ=255)
                                                                                    gACGCGGCCAGAGTTCTGGCGGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAAtt               >  2:1693723/1‑100 (MQ=38)
                                                                                    gACGCGGCCAGAGTTCTGGCGGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAAtt               >  1:795494/1‑100 (MQ=38)
                                                                                      cgcggcCAGAGTTCTGGCGGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGTCAGCGTCTGCGCGTGCCAAATGAAATTcg             >  1:3780500/1‑100 (MQ=255)
                                                                                      cgcggcCAGAGTTCTGGCGGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTcg             >  1:4339944/1‑100 (MQ=255)
                                                                                      cgcggcCAGAGTTCTGGCGGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTcg             >  2:2199485/1‑100 (MQ=255)
                                                                                       gccgccAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTcgc            <  1:2427/100‑1 (MQ=255)
                                                                                        ccgccAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTcgcg           <  1:18237/100‑1 (MQ=255)
                                                                                        ccgccAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTcgcg           >  1:2676794/1‑100 (MQ=255)
                                                                                         cgccAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTcgcg           >  2:716926/1‑99 (MQ=255)
                                                                                         cgccAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGa          >  2:3587762/1‑100 (MQ=255)
                                                                                           ccAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGAtt        >  2:1669639/1‑100 (MQ=255)
                                                                                           ccAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGAt         >  1:952350/1‑99 (MQ=255)
                                                                                            cAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGAttt       >  2:3828261/1‑100 (MQ=255)
                                                                                             aGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGAGAtcgg      >  1:1495441/1‑97 (MQ=255)
                                                                                                aCTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGcc   >  1:2823198/1‑100 (MQ=255)
                                                                                                 |                                                                                                   
AATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGCCA  >  NC_000913/3202589‑3202785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A