Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A16 F258 I0 R1 432 103.0 5191697 92.7% 4812703 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,202,686 C→T 23.1% L266F (CTC→TTC)  cca → fused tRNA nucleotidyl transferase/2'3'‑cyclic phosphodiesterase/2'nucleotidase and phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,202,6860CT23.1% 216.5 / 61.4 117L266F (CTC→TTC) ccafused tRNA nucleotidyl transferase/2'3'‑cyclic phosphodiesterase/2'nucleotidase and phosphatase
Reads supporting (aligned to +/- strand):  ref base C (48/42);  new base T (12/15);  total (60/57)
Fisher's exact test for biased strand distribution p-value = 5.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.59e-01

Only 100 of 119 total aligned reads displayed.

TTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGCC  >  NC_000913/3202587‑3202784
                                                                                                   |                                                                                                  
ttAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAAc                                                                                                    <  2:2418406/100‑1 (MQ=255)
   aTGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAActct                                                                                                 >  1:1954737/1‑100 (MQ=255)
    tGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTg                                                                                                <  2:1670915/100‑1 (MQ=255)
      aCGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGc                                                                                              >  1:2361361/1‑100 (MQ=255)
      aCGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGc                                                                                              <  2:636050/100‑1 (MQ=255)
       cGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGcc                                                                                             >  2:2241487/1‑100 (MQ=255)
         ctctCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCGGCCAGAGTTCTggcggc                                                                                           <  2:784570/100‑1 (MQ=255)
         ctctCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCcgc                                                                                           >  2:2956/1‑100 (MQ=255)
          tctcAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCcgcg                                                                                          >  2:412164/1‑100 (MQ=255)
            tcAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCGGCCAGAGTTCTGGCGGCGtc                                                                                        <  2:2522334/100‑1 (MQ=38)
             cAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGtca                                                                                       <  2:1655530/100‑1 (MQ=255)
               aTGGCGGCGATGTTGCGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCGGCCAGAGTTCTGGCGGCGtcagc                                                                                     <  2:1738054/100‑3 (MQ=37)
               aTGGCGGCGATGTTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCGGCCAGAGTTCTGGCGGCGtcagc                                                                                     <  1:13584/100‑3 (MQ=37)
               aTGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCGGCCAGAGTTCTGGCGGCGtcagc                                                                                     <  2:1223158/100‑3 (MQ=38)
               aTGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCGGCCAGAGTTCTGGCGGCGtcagc                                                                                     <  2:2392177/100‑3 (MQ=38)
               aTGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACGTTATGCCACGATCTCGGTAAAGGGCTGACGCGGCCAGAGTTCTGGCGGCGtcagc                                                                                     <  1:1977533/100‑3 (MQ=37)
                tGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGtcatca                                                                                    >  2:1898860/1‑100 (MQ=255)
                 ggcgtggATGCTGCGCCCGCAGGGCGATTTCCGTCTCGAAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCGGCCAGAGTTCTGGCGGCGgcagccc                                                                                   <  1:1877898/94‑5 (MQ=16)
                 ggcggcGATGCTGAGTCCGCAGGTCTATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCGGCCAGAGTTCTGGCGGCGtcagc                                                                                     <  1:1084185/98‑3 (MQ=37)
                 ggcggcGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTcatcat                                                                                   >  2:575501/1‑100 (MQ=255)
                 ggcggcGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTcatcat                                                                                   >  2:582507/1‑100 (MQ=255)
                 ggcggcGATGCGGAGTCCGCAGGGCGATGGCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCGGCCAGAGTTCTGGCGGCGtcagc                                                                                     <  1:1347682/98‑3 (MQ=25)
                  gcggcgATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCGGCCAGAGTTCTGGCGGCGtcagccct                                                                                  <  2:2158312/100‑6 (MQ=37)
                  gcggcgATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCGGCCAGAGTTCTGGCGGCGtcagccct                                                                                  <  2:2259170/100‑6 (MQ=37)
                  gcggcgATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCAtcatg                                                                                  <  1:1342359/100‑1 (MQ=255)
                   cggcgATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTACAGGGCTGACGCCGCCACAACTCTGTCCGCGTCAtcacgg                                                                                 >  1:2368581/1‑100 (MQ=255)
                   cggcgATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCAtcatgg                                                                                 >  2:769425/1‑100 (MQ=255)
                   cggcgATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCAtcatgg                                                                                 <  2:2503811/100‑1 (MQ=255)
                   cggcgACGCTGAGTCCGCAGGTGGATGTCCGGGTCGCAACTTGATGCCTCGATCGCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCAtcatgg                                                                                 <  1:2496892/100‑1 (MQ=255)
                       gaggCTGAGTCCACAGGTCGATGTTAGTTTCACAACTTTAGCCCACGATCTCGCTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCAtcatggt                                                                                <  1:603220/94‑1 (MQ=255)
                        aTGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCAtcatggtcatg                                                                            >  1:577512/1‑100 (MQ=255)
                           cTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGcc                                                                         <  1:388396/100‑1 (MQ=255)
                           cTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGcc                                                                         <  1:41946/100‑1 (MQ=255)
                           cTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGcc                                                                         <  2:1185817/100‑1 (MQ=255)
                           cTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGcc                                                                         >  2:2251008/1‑100 (MQ=255)
                           cTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGcc                                                                         >  2:2498935/1‑100 (MQ=255)
                            tGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGccc                                                                        >  2:1366461/1‑100 (MQ=255)
                            tGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGcc                                                                         <  2:893263/99‑1 (MQ=255)
                             gAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGccag                                                                       >  1:1185813/1‑98 (MQ=255)
                             gAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCcg                                                                       <  2:921547/100‑1 (MQ=255)
                                 ccGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCcggcgg                                                                   <  2:1834075/100‑1 (MQ=255)
                                  cGCAGGTAGATGTCCGTTTCGCAACTTTATGCCACGAACTCGGTAAAGGGCTGACGCCGGCAGAACTCTGGCCGGGTCATCCTGGTCATCGCCcggcg                                                                    >  1:1878839/1‑98 (MQ=255)
                                      ggTCGATGTCCGTTTCGCAACTTTATGCCACTATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGgtgt                                                               <  1:1209230/99‑1 (MQ=255)
                                      ggTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGgtg                                                                <  1:1256820/98‑1 (MQ=255)
                                       gTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTa                                                             <  1:1553480/100‑1 (MQ=255)
                                       gTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTa                                                             <  2:1616634/100‑1 (MQ=255)
                                       gTCGATGTCCGTTTCGCAACTTGATGCCCCGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGgtg                                                                <  1:900865/97‑1 (MQ=255)
                                        tCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTaa                                                            <  2:1208016/100‑1 (MQ=255)
                                          gATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGt                                                          >  1:584508/1‑100 (MQ=255)
                                             gTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAg                                                       >  1:1063849/1‑100 (MQ=255)
                                               ccGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTg                                                     <  2:1606981/100‑1 (MQ=255)
                                                   ttCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGaac                                                 >  1:1382065/1‑100 (MQ=255)
                                                     cGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGaacaa                                               >  2:1910552/1‑100 (MQ=255)
                                                      gCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCTGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGaacaac                                              <  2:790973/100‑1 (MQ=255)
                                                      gCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGaacaac                                              >  1:1938432/1‑100 (MQ=255)
                                                      gCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGaacaac                                              <  2:2081170/100‑1 (MQ=255)
                                                      gCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCAGCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGaacaac                                              <  2:1943725/100‑1 (MQ=255)
                                                          cTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATg                                          >  1:1167744/1‑100 (MQ=255)
                                                          cTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATg                                          >  1:1011922/1‑100 (MQ=255)
                                                           tttATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGc                                         <  2:2305014/100‑1 (MQ=255)
                                                            ttATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGcc                                        >  1:2552981/1‑100 (MQ=255)
                                                            ttATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTATGGCCGCGTCATCATGGTCATGGCCCGGCGTGTGTTAAGTTAGTGGAACCACTATgac                                        >  1:1776156/1‑98 (MQ=255)
                                                               tGCCACGATCTGGGGCGAGGGCTGAGACAGCAAGAGCTCAGGCCGCGCAACCATGTTCTTTTCTTGTTGCGTGTTAAGTTAGTGGAACAACTATGCTAGc                                     >  1:2240427/1‑100 (MQ=255)
                                                                gCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCg                                    >  2:1441315/1‑100 (MQ=255)
                                                                gCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCg                                    <  1:590391/100‑1 (MQ=255)
                                                                gCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCg                                    >  1:449478/1‑100 (MQ=255)
                                                                gCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACGCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCg                                    >  2:1829828/1‑100 (MQ=255)
                                                                      aTCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTgcg                              <  1:1707475/100‑1 (MQ=255)
                                                                       tctcGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGGCTgcgc                             >  2:2118255/1‑100 (MQ=255)
                                                                            gTAAAGGGCTTACGCCGCCAGAACTGTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGcc                        <  1:1694372/100‑1 (MQ=255)
                                                                            gTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGcc                        >  2:1181540/1‑100 (MQ=255)
                                                                             tAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCa                       >  2:2330966/1‑100 (MQ=255)
                                                                              aaaGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGAAACAACTATGCCAGCGTCTGCGCGTGCCaa                      >  1:1423376/1‑100 (MQ=255)
                                                                               aaGGGCTGACGCGGCCAGAACGCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGGTAAAGTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCan                      <  1:72479/99‑2 (MQ=255)
                                                                                 gggCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATg                   >  2:1459679/1‑100 (MQ=255)
                                                                                 atgatGACGCGGCCAGAGTTCTGGCGGCGTCATCATGGTCATGGCCCGGCGGGTGTTTAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATg                   >  1:1232279/5‑100 (MQ=37)
                                                                                  ggCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGa                  >  1:2409555/1‑100 (MQ=255)
                                                                                   gCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGaa                 >  2:1302991/1‑100 (MQ=255)
                                                                                    atGACGCGGCCAGAGTTCTGGCGGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGaaa                >  2:1479325/2‑100 (MQ=38)
                                                                                     tGACGCGGCCAGAGTTCTGGCGGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAAt               >  2:745917/1‑100 (MQ=38)
                                                                                      gACGCGGCCAGAGTTCTGGCGGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAAtt              >  2:2080262/1‑100 (MQ=38)
                                                                                      gACGCGGCCAGAGTTCTGGCGGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAAtt              >  1:1166125/1‑100 (MQ=38)
                                                                                      gACGCGGCCAGAGTTCTGGCGGCGTCAGCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAAtt              >  2:2480842/1‑100 (MQ=37)
                                                                                      gACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAAtt              >  2:2575987/1‑100 (MQ=255)
                                                                                       aCGCGGCCAGAGTTCTGGCGGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTc             >  1:2326226/1‑100 (MQ=39)
                                                                                        cgcggcCAGAGTTCTGGCGGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTcg            >  2:2169900/1‑100 (MQ=255)
                                                                                        cgcggcCAGAGTTCTGGCGGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTcg            >  2:2163279/1‑100 (MQ=255)
                                                                                        cgcggcCAGAGTTCTGGCGGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTcg            >  2:1718602/1‑100 (MQ=255)
                                                                                        cgcggcCAGAGTTCTGGCGGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTcg            >  2:1516218/1‑100 (MQ=255)
                                                                                        cgccgcCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTcg            >  1:1536289/1‑100 (MQ=255)
                                                                                        cgccgcCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTcg            >  1:122644/1‑100 (MQ=255)
                                                                                        cgccgcCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTcg            >  1:736113/1‑100 (MQ=255)
                                                                                        cgccgcCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTcg            <  1:1080916/100‑1 (MQ=255)
                                                                                         gccgccAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTcgc           <  1:171725/100‑1 (MQ=255)
                                                                                          ccgccAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTcgcg          <  1:2569930/100‑1 (MQ=255)
                                                                                            gccAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGAt        <  2:2428634/100‑1 (MQ=255)
                                                                                            gccAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGAt        <  2:2207159/100‑1 (MQ=255)
                                                                                              cAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGAttt      >  1:522827/1‑100 (MQ=255)
                                                                                                 aaCTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGc   <  2:1063853/100‑1 (MQ=255)
                                                                                                  aCTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGcc  >  1:839687/1‑100 (MQ=255)
                                                                                                   |                                                                                                  
TTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGCC  >  NC_000913/3202587‑3202784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A