Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F232 I0 R1 243 156.3 7646460 93.8% 7172379 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,411,299 T→C 100% V10A (GTA→GCA)  fis → global DNA‑binding transcriptional dual regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,411,2990TC99.4% 583.7 / ‑5.1 166V10A (GTA→GCA) fisglobal DNA‑binding transcriptional dual regulator
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (75/90);  total (76/90)
Fisher's exact test for biased strand distribution p-value = 4.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.51e-01

Only 100 of 166 total aligned reads displayed.

ACAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAAC  >  NC_000913/3411203‑3411394
                                                                                                |                                                                                               
aCAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACt                                                                                              >  2:147298/1‑100 (MQ=255)
aCAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACt                                                                                              >  2:145939/1‑100 (MQ=255)
aCAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACt                                                                                              <  1:47229/100‑1 (MQ=255)
aCAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACt                                                                                              <  1:521719/100‑1 (MQ=255)
 cAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTg                                                                                             >  1:1568205/1‑100 (MQ=255)
   gCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGAc                                                                                           >  1:613783/1‑100 (MQ=255)
   gCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGAc                                                                                           >  1:909026/1‑100 (MQ=255)
   gCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGAc                                                                                           >  1:1555703/1‑100 (MQ=255)
      ggAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGt                                                                                        >  2:1304995/1‑100 (MQ=255)
       gAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGtt                                                                                       >  1:1718007/1‑100 (MQ=255)
           cGTTGTTGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAATTATGTTCTAACAACGCGTAAATTCTGACGCACTGACCGTTTCTa                                                                                   >  2:68097/1‑100 (MQ=255)
           cGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTa                                                                                   >  1:2287087/1‑100 (MQ=255)
            gTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTan                                                                                  <  1:3499634/100‑2 (MQ=255)
             ttGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTAcc                                                                                 <  1:2610252/100‑1 (MQ=255)
               ggAGTCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGt                                                                               <  2:2812923/100‑1 (MQ=255)
                gAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGtt                                                                              >  1:1643150/1‑100 (MQ=255)
                gAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGtt                                                                              <  1:3644503/100‑1 (MQ=255)
                gAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGtt                                                                              <  1:1820649/100‑1 (MQ=255)
                 aGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTa                                                                             <  1:2809684/100‑1 (MQ=255)
                 aGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTa                                                                             <  1:995800/100‑1 (MQ=255)
                  ggCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTaa                                                                            >  2:100485/1‑100 (MQ=255)
                   gCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAAc                                                                           <  2:2158829/100‑1 (MQ=255)
                    cATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTCACAAAACTATGTTCGAACAACGCGTAAATTCTAACGCACTGACCGTTTCTACAGTTAAct                                                                          >  2:3232256/1‑100 (MQ=255)
                     aTACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAActc                                                                         <  2:717788/100‑1 (MQ=255)
                        cTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCa                                                                      >  2:3471650/1‑100 (MQ=255)
                         ttCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAg                                                                     >  1:3313876/1‑100 (MQ=255)
                         ttCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAg                                                                     <  1:2026568/100‑1 (MQ=255)
                         ttCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAg                                                                     <  2:1054799/100‑1 (MQ=255)
                         ttCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAg                                                                     <  1:295363/100‑1 (MQ=255)
                              aacttttGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCa                                                                <  1:3043246/97‑1 (MQ=255)
                              aaaTTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCa                                                                <  2:796018/100‑1 (MQ=255)
                              aaaTTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCa                                                                <  2:3405686/100‑1 (MQ=255)
                               aaTTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCan                                                               <  1:1900394/100‑2 (MQ=255)
                               aaTTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAg                                                               <  1:1528079/100‑1 (MQ=255)
                                 ttttGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGt                                                             <  2:812445/100‑1 (MQ=255)
                                 ttttGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGt                                                             >  2:1558543/1‑100 (MQ=255)
                                   ttGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTaa                                                           <  2:3096440/100‑1 (MQ=255)
                                   ttGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTaa                                                           >  2:1942501/1‑100 (MQ=255)
                                     gCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAAcc                                                         >  1:1415981/1‑100 (MQ=255)
                                      cGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAAccc                                                        >  2:2713496/1‑100 (MQ=255)
                                      cGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAAccc                                                        >  1:2867144/1‑100 (MQ=255)
                                      cGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAAccc                                                        >  1:2262901/1‑100 (MQ=255)
                                      cGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAAccc                                                        >  1:1959530/1‑100 (MQ=255)
                                        tAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCaa                                                      >  2:3413604/1‑100 (MQ=255)
                                        tAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCAAAAATTCTGACGCACTGACCGTTTCTACCGCTAACTCTCAGGCTCAGGTAACCCaa                                                      >  2:916743/1‑100 (MQ=255)
                                         aaaCAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAGATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCaaa                                                     >  1:2203687/1‑100 (MQ=255)
                                         aaaCAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAggagatcggaa                                                     >  1:1469143/1‑91 (MQ=255)
                                         aaaCAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCaaa                                                     <  1:1325779/100‑1 (MQ=255)
                                          aaCAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCaaaa                                                    >  2:3578559/1‑100 (MQ=255)
                                           ncAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCaaaaa                                                   >  1:1973236/2‑100 (MQ=255)
                                           aCAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCaaaan                                                   <  2:2256807/100‑2 (MQ=255)
                                           aCAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCaaaaa                                                   >  1:760728/1‑100 (MQ=255)
                                            cAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAAc                                                  >  2:666218/1‑100 (MQ=255)
                                            cAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAAc                                                  <  2:2103904/100‑1 (MQ=255)
                                            cAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAAc                                                  >  1:1787546/1‑100 (MQ=255)
                                              gAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAAccc                                                >  1:1117570/1‑100 (MQ=255)
                                               aaaTAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAAcccc                                               <  2:1169395/100‑1 (MQ=255)
                                                aaTAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTTACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAAcc                                                 <  2:2802101/97‑1 (MQ=255)
                                                 aTAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTg                                             >  2:1909064/1‑100 (MQ=255)
                                                   aaaGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCg                                           <  2:3784577/100‑1 (MQ=255)
                                                    aaGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCGACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCGGCGt                                          <  2:3326021/100‑1 (MQ=255)
                                                        gCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGCTAACTCTCCGGATCAGGTAACCCAAAAACCCCTGCGTGACt                                      >  2:96003/1‑100 (MQ=255)
                                                          tGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCg                                    >  1:1203237/1‑100 (MQ=255)
                                                           gacgaaaCTATGTTCGAACAACGCTTAATTTCTAACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCgg                                   <  2:2497015/95‑1 (MQ=255)
                                                           gACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCgg                                   <  1:2590268/100‑1 (MQ=255)
                                                           gACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCgg                                   <  1:286220/100‑1 (MQ=255)
                                                            aCAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGt                                  <  2:42139/100‑1 (MQ=255)
                                                            aCAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGt                                  <  2:103506/100‑1 (MQ=255)
                                                              aGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTa                                <  1:2194738/100‑1 (MQ=255)
                                                              aGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTa                                >  1:3053343/1‑100 (MQ=255)
                                                               gAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCCAAAACCCCTGCGTGACTCGGTTaa                               >  2:853104/1‑100 (MQ=255)
                                                               gAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTaa                               >  1:3228586/1‑100 (MQ=255)
                                                               gAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTAACGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTaa                               >  2:1331658/1‑100 (MQ=255)
                                                                 gatctGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAAc                             <  1:380122/96‑1 (MQ=255)
                                                                 aCTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAAc                             <  2:3791118/100‑1 (MQ=255)
                                                                    aTGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAgg                          <  1:519515/100‑1 (MQ=255)
                                                                      gTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAgatc                        >  2:380120/1‑97 (MQ=255)
                                                                      gTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCa                        <  2:893017/100‑1 (MQ=255)
                                                                          gAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTga                    <  1:885297/100‑1 (MQ=255)
                                                                            acaacGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTgaag                  <  1:3794254/100‑1 (MQ=255)
                                                                            acaacGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTgaag                  <  1:2979697/100‑1 (MQ=255)
                                                                             caacGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTgaaga                 <  2:1203228/100‑1 (MQ=255)
                                                                               acGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAGAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAAc               <  2:1685838/100‑1 (MQ=255)
                                                                                cgcgTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACt              >  2:2571593/1‑100 (MQ=255)
                                                                                 gcgTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTa             <  2:1761807/100‑1 (MQ=255)
                                                                                  cgTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGAAAAAAAAAAAAAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTAt            <  2:2780295/100‑1 (MQ=255)
                                                                                   gTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTAtt           >  1:166220/1‑100 (MQ=255)
                                                                                    tAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTAttt          >  1:148423/1‑100 (MQ=255)
                                                                                     aaaTTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTAtttt         >  2:3112579/1‑100 (MQ=255)
                                                                                      aaTTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTg        <  1:1228533/100‑1 (MQ=255)
                                                                                       aTTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGc       >  1:1133825/1‑100 (MQ=255)
                                                                                       aTTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGc       >  1:2348165/1‑100 (MQ=255)
                                                                                       aTTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGc       <  2:1631672/100‑1 (MQ=255)
                                                                                        ttCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGCt      >  2:1107153/1‑100 (MQ=255)
                                                                                          cTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCa    <  1:249747/100‑1 (MQ=255)
                                                                                          cTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCa    >  2:1399429/1‑100 (MQ=255)
                                                                                          cTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCa    <  2:148423/100‑1 (MQ=255)
                                                                                           tGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCaa   <  2:2005196/100‑1 (MQ=255)
                                                                                            gACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAAc  >  1:2850268/1‑100 (MQ=255)
                                                                                            gACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAAc  >  1:2932171/1‑100 (MQ=255)
                                                                                                |                                                                                               
ACAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAAC  >  NC_000913/3411203‑3411394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A