Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F255 I0 R1 121 91.8 4500463 95.1% 4279940 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,411,299 T→C 100% V10A (GTA→GCA)  fis → global DNA‑binding transcriptional dual regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,411,2990TC98.2% 350.1 / ‑3.2 106V10A (GTA→GCA) fisglobal DNA‑binding transcriptional dual regulator
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (47/57);  total (48/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.82e-01

Only 100 of 106 total aligned reads displayed.

AACAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAA  >  NC_000913/3411202‑3411393
                                                                                                 |                                                                                              
aaCAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGTAc                                                                                              >  2:841442/1‑100 (MQ=255)
  cAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACt                                                                                             >  2:1527743/1‑99 (MQ=255)
     cTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGAcc                                                                                         <  1:122168/100‑1 (MQ=255)
      tGGAGGCGTTGGAGGCATACTTCGAAAAGTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGAcc                                                                                         <  2:1544693/99‑1 (MQ=255)
        gAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGtt                                                                                      <  1:2028879/100‑1 (MQ=255)
         aGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGttt                                                                                     >  1:350558/1‑100 (MQ=255)
          ggCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTc                                                                                    <  1:906542/100‑1 (MQ=255)
          ggCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTc                                                                                    <  2:1446290/100‑1 (MQ=255)
           gCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCt                                                                                   <  1:1932513/100‑1 (MQ=255)
           gCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCt                                                                                   <  1:583444/100‑1 (MQ=255)
           gCGTTGGAGGCATACTTCGAAAATTTTGCGGAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCt                                                                                   <  1:1887228/100‑1 (MQ=255)
            cGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTa                                                                                  <  2:1446318/100‑1 (MQ=255)
               tGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCg                                                                               >  1:2021379/1‑100 (MQ=255)
               tGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCg                                                                               <  1:1270006/100‑1 (MQ=255)
                ggAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGt                                                                              >  2:22439/1‑100 (MQ=255)
                ggAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGt                                                                              <  2:2040616/100‑1 (MQ=255)
                ggAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGt                                                                              <  1:1977271/100‑1 (MQ=255)
                  aGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGtt                                                                             <  2:512431/99‑1 (MQ=255)
                   ggCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTaa                                                                           >  1:65660/1‑100 (MQ=255)
                    gCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAAc                                                                          <  1:474019/100‑1 (MQ=255)
                     cATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAAct                                                                         >  1:573228/1‑100 (MQ=255)
                       tACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCTAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAAct                                                                         >  2:825879/1‑98 (MQ=255)
                         cTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCa                                                                     <  2:1623567/100‑1 (MQ=255)
                         cTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACCATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCa                                                                     <  2:1434651/100‑1 (MQ=255)
                          ttCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAg                                                                    >  2:896616/1‑100 (MQ=255)
                           tCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAgg                                                                   <  1:190462/100‑1 (MQ=255)
                            cGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAgta                                                                  >  2:1215922/1‑98 (MQ=255)
                            cGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGa                                                                  >  1:1198056/1‑100 (MQ=255)
                            cGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGa                                                                  >  1:1100783/1‑100 (MQ=255)
                               aaaTTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCa                                                               <  1:693047/100‑1 (MQ=255)
                               aaaTTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCa                                                               <  2:1865695/100‑1 (MQ=255)
                                  ttttGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGt                                                            >  1:1080580/1‑100 (MQ=255)
                                  ttttGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGt                                                            <  2:65660/100‑1 (MQ=255)
                                       cGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTaa                                                          >  1:1759465/1‑97 (MQ=255)
                                       cGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAAccc                                                       >  1:627748/1‑100 (MQ=255)
                                       cGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTCTCTACCGTTAACTCTCAGGATCAGGTAAccc                                                       >  2:1833407/1‑100 (MQ=255)
                                        gTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCa                                                      >  2:1247477/1‑100 (MQ=255)
                                         tAAACAGAATTTAAGAGCTGAGAGAATTATGTTAGAACAACGCGTAAATTGTGACGCACTGACAGTTTCTATAGTTAACTCTCAGGATCAGGTaagc                                                        >  2:1003841/1‑95 (MQ=255)
                                         tAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAATGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCaa                                                     >  2:1957941/1‑100 (MQ=255)
                                         tAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCaa                                                     >  1:1139247/1‑100 (MQ=255)
                                           aaCAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCaaaa                                                   >  2:1815642/1‑100 (MQ=255)
                                           aaCAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCaaaa                                                   >  1:1989194/1‑100 (MQ=255)
                                            aCAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCaaaaa                                                  >  1:8650/1‑100 (MQ=255)
                                            aCAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCaaaaa                                                  <  2:681240/100‑1 (MQ=255)
                                            aCAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCATAAATTCTGACGCGCTGACCGTTTCTACCCTTAACTCTCAGCATCACGTCACCCaaca                                                   >  2:2119695/1‑97 (MQ=255)
                                             cAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAAc                                                 >  1:158660/1‑100 (MQ=255)
                                             cAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAAc                                                 >  2:1410413/1‑100 (MQ=255)
                                               gAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAAccc                                               <  2:8650/100‑1 (MQ=255)
                                               gAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAAccc                                               <  1:1634132/100‑1 (MQ=255)
                                               gAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAAccc                                               >  2:406134/1‑100 (MQ=255)
                                               gAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAAccc                                               >  2:2190057/1‑100 (MQ=255)
                                                aaataaatAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCaaaaa                                                  <  1:566060/96‑1 (MQ=255)
                                                aaaTAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAAcccc                                              <  1:808926/100‑1 (MQ=255)
                                                  aTAAAAAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTg                                            <  2:1553414/100‑1 (MQ=255)
                                                     aaGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGt                                         <  1:1539699/100‑1 (MQ=255)
                                                     aaGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGt                                         <  2:2093067/100‑1 (MQ=255)
                                                      agagCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTg                                        <  1:1396017/100‑1 (MQ=255)
                                                      agagCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTg                                        <  1:682387/100‑1 (MQ=255)
                                                      agagCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTGAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTg                                        <  2:335977/100‑1 (MQ=255)
                                                       gagCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGa                                       <  2:1565288/100‑1 (MQ=255)
                                                          cTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTc                                    >  2:710676/1‑100 (MQ=255)
                                                            gACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCgg                                  >  2:1353885/1‑100 (MQ=255)
                                                            gACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCgg                                  >  2:804938/1‑100 (MQ=255)
                                                             aCAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGt                                 >  1:1463675/1‑100 (MQ=255)
                                                              cAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGtt                                <  1:1979266/100‑1 (MQ=255)
                                                              cAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGtt                                >  1:2226052/1‑100 (MQ=255)
                                                               aGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATAAGGTAACACAAAAACCCCTGCGTGACTCGGt                                 <  2:833801/98‑1 (MQ=255)
                                                                gAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTaa                              >  2:392877/1‑100 (MQ=255)
                                                                gAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTaa                              <  2:1820351/100‑1 (MQ=255)
                                                                gAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTATCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGtt                                >  2:1864678/1‑98 (MQ=255)
                                                                  aCTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAAc                            <  1:389872/100‑1 (MQ=255)
                                                                  aCTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAAc                            <  1:2114324/100‑1 (MQ=255)
                                                                  aCTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAAc                            <  1:2148090/100‑1 (MQ=255)
                                                                    tATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTGTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACGCGGTTAAAc                            <  2:490458/98‑1 (MQ=255)
                                                                      tGTTCGAACAACGGGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGc                        <  2:1270079/100‑1 (MQ=255)
                                                                      tGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGc                        <  1:758382/100‑1 (MQ=255)
                                                                      tGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGc                        <  2:339941/100‑1 (MQ=255)
                                                                       gTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCa                       >  2:719639/1‑100 (MQ=255)
                                                                          cGAACACCGCGTAAATTCTGACGCACTGACCGTGTCCACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACt                     <  2:2244713/99‑1 (MQ=255)
                                                                          cGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTg                    >  2:762728/1‑100 (MQ=255)
                                                                           gAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGTATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTga                   <  1:1215926/100‑1 (MQ=255)
                                                                           gAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATAAAATAAACAAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTga                   <  2:1662355/100‑1 (MQ=255)
                                                                             acaacGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTgaag                 <  1:1833419/100‑1 (MQ=255)
                                                                               aacGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTgaagaa               <  1:710676/100‑1 (MQ=255)
                                                                               aacGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTgaagaa               <  1:2011916/100‑1 (MQ=255)
                                                                                acGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAAc              <  1:1576576/100‑1 (MQ=255)
                                                                                acGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAAc              >  1:33197/1‑100 (MQ=255)
                                                                                 cgcgTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACt             >  1:1382945/1‑100 (MQ=255)
                                                                                 cgcgTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACt             >  1:1024473/1‑100 (MQ=255)
                                                                                 cgcgTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACt             >  1:1813426/1‑100 (MQ=255)
                                                                                  gcgTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTAAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACAGAAGAACTa            >  2:550539/1‑100 (MQ=255)
                                                                                   cgTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTAt           >  1:897969/1‑100 (MQ=255)
                                                                                       aaTTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTg       <  2:559562/100‑1 (MQ=255)
                                                                                       aaTTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTg       <  2:1683140/100‑1 (MQ=255)
                                                                                          tCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGCTc    <  2:1776882/100‑1 (MQ=255)
                                                                                          tCTGACGCACTGACCGTTGCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGCTc    <  1:1039581/100‑1 (MQ=255)
                                                                                           cTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGCTc    >  1:2165945/1‑99 (MQ=255)
                                                                                           cTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCa   >  2:783920/1‑100 (MQ=255)
                                                                                           cTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCa   >  1:1125980/1‑100 (MQ=255)
                                                                                             gACGCACTGACCGTTTCTACCGTTAACTCTCAGGATTAAGTAATCCAAAAACCCCTTCTTGACTCGGTTAATCACTCACTTAAGAACTATTTTGCTc    <  2:1302874/97‑1 (MQ=255)
                                                                                                 |                                                                                              
AACAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAA  >  NC_000913/3411202‑3411393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A