Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A16 F258 I0 R1 432 103.0 5191697 92.7% 4812703 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,411,299 T→C 100% V10A (GTA→GCA)  fis → global DNA‑binding transcriptional dual regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,411,2990TC97.2% 343.7 / ‑0.5 105V10A (GTA→GCA) fisglobal DNA‑binding transcriptional dual regulator
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base C (46/56);  total (47/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.65e-01

Only 100 of 105 total aligned reads displayed.

AACAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAAC  >  NC_000913/3411202‑3411394
                                                                                                 |                                                                                               
aaCAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCAc                                                                                               <  1:541601/100‑1 (MQ=255)
  cAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAACTTCTGACGCACTg                                                                                             >  1:792561/1‑100 (MQ=255)
  cAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTg                                                                                             >  1:566006/1‑100 (MQ=255)
  cAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTg                                                                                             <  1:107091/100‑1 (MQ=255)
    gCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGa                                                                                            >  1:1952847/1‑99 (MQ=255)
    gCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGAc                                                                                           >  1:1866750/1‑100 (MQ=255)
    gCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGAc                                                                                           <  2:1158593/100‑1 (MQ=255)
    gCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGAc                                                                                           >  2:1305259/1‑100 (MQ=255)
     cTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGAcc                                                                                          >  1:966670/1‑100 (MQ=255)
     cTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGAcc                                                                                          <  1:1919257/100‑1 (MQ=255)
          ggCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTc                                                                                     <  1:2187697/100‑1 (MQ=255)
          ggCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTc                                                                                     >  1:1989967/1‑100 (MQ=255)
          ggCGTTGGAGGCATACCTCGAAAATTTTGCGTAAACAGAAATAACGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGAAGCACTGCCCGttt                                                                                      >  1:1083676/1‑99 (MQ=255)
           gCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCt                                                                                    <  1:1054280/100‑1 (MQ=255)
           gCGTTGGAGGCATACTTCGAAAACCTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTc                                                                                     <  1:2188087/99‑1 (MQ=255)
            cGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTa                                                                                   <  2:457033/100‑1 (MQ=255)
            cGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTa                                                                                   <  2:248739/100‑1 (MQ=255)
                gtatgCATACTTCTAAAATTTTTCTTAAGCAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTTACGCACTGACCGTTTCTACCg                                                                                <  1:2422443/95‑1 (MQ=255)
                ggAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGATCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGt                                                                               <  1:1170420/100‑1 (MQ=255)
                 gAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGt                                                                               <  1:1517709/99‑1 (MQ=255)
                  aGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTa                                                                             <  1:1124781/100‑1 (MQ=255)
                   ggCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGCACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTaa                                                                            >  1:1434569/1‑100 (MQ=255)
                     cATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAAc                                                                           >  2:2092912/1‑99 (MQ=255)
                        aCTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACtctc                                                                       <  2:332461/100‑1 (MQ=255)
                         cTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCa                                                                      <  1:1092998/100‑1 (MQ=255)
                          ttCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAg                                                                     >  2:1379873/1‑100 (MQ=255)
                           tCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAgg                                                                    <  1:1229825/100‑1 (MQ=255)
                            cGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGa                                                                   <  2:1451221/100‑1 (MQ=255)
                            cGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGa                                                                   >  2:1801445/1‑100 (MQ=255)
                             gAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGAt                                                                  >  1:1290834/1‑100 (MQ=255)
                              aaaaTTTTGCGTAAACAGAAATAAAGATCTGACAGAACTATGTTCTAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACtctc                                                                       <  1:2080445/94‑1 (MQ=255)
                              aaaaTTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATc                                                                 >  2:876631/1‑100 (MQ=255)
                                aaTTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAg                                                               >  2:1774630/1‑100 (MQ=255)
                                 aTTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAgg                                                              >  1:544749/1‑100 (MQ=255)
                                  ttttGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACTGTTAACTCTCAGGATCAGGt                                                             <  2:2438015/100‑1 (MQ=255)
                                  ttttGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGt                                                             >  1:190108/1‑100 (MQ=255)
                                  ttttGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGCTTCTACCGTTAACTCTCAGGATCAGGt                                                             <  2:1614588/100‑1 (MQ=255)
                                   tttGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTa                                                            <  1:2350109/100‑1 (MQ=255)
                                      gCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAAcc                                                         <  1:383874/100‑1 (MQ=255)
                                      gCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAAcc                                                         <  2:2382255/100‑1 (MQ=255)
                                       cGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAAccc                                                        <  1:236230/100‑1 (MQ=255)
                                       cGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAAccc                                                        >  2:1369702/1‑100 (MQ=255)
                                       cGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAAccc                                                        <  2:2113615/100‑1 (MQ=255)
                                        gTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCa                                                       >  2:936342/1‑100 (MQ=255)
                                        gTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCa                                                       >  2:1230855/1‑100 (MQ=255)
                                        gTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCa                                                       <  2:1952857/100‑1 (MQ=255)
                                        gTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCa                                                       <  2:877693/100‑1 (MQ=255)
                                        gTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAAAAATGCGTAAATTCTGACGCACTGACTGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCa                                                       >  1:1067419/1‑100 (MQ=255)
                                         tAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCaa                                                      >  1:139316/1‑100 (MQ=255)
                                          aaaCAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCaaa                                                     >  2:1973976/1‑100 (MQ=255)
                                           aaCAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCaaaa                                                    >  2:452582/1‑100 (MQ=255)
                                            aCAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTTACGCACTTACCGATTATACCGTTAACTCTCAGGATCAGGTAACCCaaaaa                                                   >  1:1203096/1‑100 (MQ=255)
                                            aCAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCaaaaa                                                   >  2:906591/1‑100 (MQ=255)
                                            aCAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCaaaaa                                                   >  1:1728126/1‑100 (MQ=255)
                                             cAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAAc                                                  >  2:531746/1‑100 (MQ=255)
                                              aGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAAcc                                                 <  1:695954/100‑1 (MQ=255)
                                               gAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAAccc                                                >  2:2564231/1‑100 (MQ=255)
                                               gAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAAccc                                                >  2:1651198/1‑100 (MQ=255)
                                                  aTAAAGTGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTg                                             <  2:67090/100‑1 (MQ=255)
                                                    aaaGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTGTACCGTTAACTCTCAGGATCAGGTAGCCCAAAAACCCCTGCg                                           <  1:324727/100‑1 (MQ=255)
                                                    aaaGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCg                                           <  1:324356/100‑1 (MQ=255)
                                                      agagCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTg                                         <  1:1008385/100‑1 (MQ=255)
                                                          cTGACAGAACTATGTTCGAACAACGTGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAGCCCCTGCGTg                                         <  1:811278/96‑1 (MQ=255)
                                                            gACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCgg                                   <  2:795251/100‑1 (MQ=255)
                                                            gACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCgg                                   >  1:1241716/1‑100 (MQ=255)
                                                            gACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCgg                                   >  2:1476379/1‑100 (MQ=255)
                                                            gACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCgg                                   >  2:649700/1‑100 (MQ=255)
                                                            gACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCgg                                   >  1:13131/1‑100 (MQ=255)
                                                            gACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCgg                                   <  2:1401042/100‑1 (MQ=255)
                                                              cAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGtt                                 <  2:629854/100‑1 (MQ=255)
                                                              cAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGtt                                 >  1:2508093/1‑100 (MQ=255)
                                                               aGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTa                                <  1:1399406/100‑1 (MQ=255)
                                                               aGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTa                                <  1:325464/100‑1 (MQ=255)
                                                                  aCTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAAc                             >  2:1667100/1‑100 (MQ=255)
                                                                   cTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACa                            <  2:529264/100‑1 (MQ=255)
                                                                    tATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAg                           <  1:2426702/100‑1 (MQ=255)
                                                                      tGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGc                         <  1:395258/100‑1 (MQ=255)
                                                                      tGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGc                         <  2:1675309/100‑1 (MQ=255)
                                                                       gTTCGAACAACGCGTAAATTCTGACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCa                        >  2:1959346/1‑100 (MQ=255)
                                                                       gTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGc                         <  1:590618/99‑1 (MQ=255)
                                                                       gTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCa                        >  2:363376/1‑100 (MQ=255)
                                                                        ttCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCAc                       <  1:1350620/100‑1 (MQ=255)
                                                                        ttCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCAc                       >  2:2517682/1‑100 (MQ=255)
                                                                        ttCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCAc                       <  2:1480802/100‑1 (MQ=255)
                                                                          cGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTg                     <  2:1728135/100‑1 (MQ=255)
                                                                           gAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTga                    >  1:2552060/1‑100 (MQ=255)
                                                                           gAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTga                    <  1:1294357/100‑1 (MQ=255)
                                                                            aacaacGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTgaa                   <  1:2359678/100‑1 (MQ=255)
                                                                             acaacGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTgaag                  <  1:185043/100‑1 (MQ=255)
                                                                              caacGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAAGTAACAAAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTgaaga                 <  1:2073478/100‑1 (MQ=255)
                                                                                acGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAAc               <  2:1434577/100‑1 (MQ=255)
                                                                                 cgcgTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACt              >  1:563751/1‑100 (MQ=255)
                                                                                   cgTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTAt            >  1:1246636/1‑100 (MQ=255)
                                                                                    gTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTAtt           >  1:1221512/1‑100 (MQ=255)
                                                                                    gTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTAtt           >  1:661925/1‑100 (MQ=255)
                                                                                     tAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTAttt          <  1:2080811/100‑1 (MQ=255)
                                                                                      aaaTTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTAtttt         >  2:1035321/1‑100 (MQ=255)
                                                                                       aaTTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTg        <  2:1633601/100‑1 (MQ=255)
                                                                                         ttCTGACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGCt      <  1:1959336/100‑1 (MQ=255)
                                                                                             gACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAAc  <  2:2437487/100‑1 (MQ=255)
                                                                                                 |                                                                                               
AACAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAAC  >  NC_000913/3411202‑3411394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A