Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F232 I0 R1	243	156.3	7646460	93.8%	7172379	99.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,488,087	A→G	22.0%	Y257C (TAT→TGT)	lptG →	lipopolysaccharide export ABC permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,488,087	0	A	G	22.0%	304.1 / 91.8	168	Y257C (TAT→TGT)	lptG	lipopolysaccharide export ABC permease
Reads supporting (aligned to +/- strand): ref base A (55/76); new base G (24/13); total (79/89)
Fisher's exact test for biased strand distribution p-value = 1.59e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.50e-01

Only 100 of 172 total aligned reads displayed.

AGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATCAGCTCAACATGTGGAGCAAAATCTTCCAGCCGCTATCTGTGGCGGTGATGATGCT  >  NC_000913/4487988‑4488186

 gACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTAt                                                                                                    <  1:296403/100‑1 (MQ=255)

  aCGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTAtg                                                                                                   <  2:1448939/100‑1 (MQ=255)

  aCGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTAtg                                                                                                   <  2:888879/100‑1 (MQ=255)

     gTGAGCGGCACCTGGAAAACCAACCTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGa                                                                                                <  2:1992729/100‑1 (MQ=255)

     gTGAGCGGCACCTGGAAAACCAACCTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGa                                                                                                >  2:1796651/1‑100 (MQ=255)

         gCGGCACCTGGAAAACCAACCTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTa                                                                                            >  1:1590681/1‑100 (MQ=255)

         gCGGCACCTGGAAAACCAACCTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTa                                                                                            <  2:374046/100‑1 (MQ=255)

         gCGGCACCTGGAAAACCAACCTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTa                                                                                            >  1:2838626/1‑100 (MQ=255)

         gCGGCACCTGCAAAACCAACCTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTa                                                                                            >  1:3374140/1‑100 (MQ=255)

           ggCACCTGGAAAACCAACCTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATc                                                                                          <  2:3067202/100‑1 (MQ=255)

              aCCTGGAAAACCAACCTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGa                                                                                       <  1:136862/100‑1 (MQ=255)

               ccTGGAAAACCAACCTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGaa                                                                                      <  1:1384811/100‑1 (MQ=255)

                cTGGAAAACCAACCTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAg                                                                                     <  2:877446/100‑1 (MQ=255)

                cTGGAAAACCAACCTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAg                                                                                     >  1:2393157/1‑100 (MQ=255)

                 tGGAAAACCAACCTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGt                                                                                    >  1:2629617/1‑100 (MQ=255)

                 tGGAAAACCAACCTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGt                                                                                    <  1:3141217/100‑1 (MQ=255)

                  ggAAAACCAACCTCACGCCGGACAACCTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTc                                                                                   >  2:3303638/1‑100 (MQ=255)

                  ggAAAACCAACCTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTc                                                                                   <  2:2878685/100‑1 (MQ=255)

                  ggAAAACCAACCTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTc                                                                                   <  2:3156360/100‑1 (MQ=255)

                   gAAAACCAACCTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTGTGTGAAGTATCTGAAGTCg                                                                                  >  1:3047792/1‑100 (MQ=255)

                   gAAAACCAACCTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCg                                                                                  >  1:2469335/1‑100 (MQ=255)

                     aaacaaacCTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGa                                                                                 >  2:389552/1‑99 (MQ=255)

                     aaaCCAACCTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAg                                                                                <  1:1002528/100‑1 (MQ=255)

                      aaccaaccTCACGCCGGACAAACTGGGCGTGGTGGCGCTGAACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGc                                                                               <  1:1923890/100‑1 (MQ=255)

                        ccaaccTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCgg                                                                             <  1:2240856/100‑1 (MQ=255)

                        ccaaccTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGAGGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCgn                                                                             <  2:2855836/100‑2 (MQ=255)

                          aaccTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTc                                                                           <  1:1814189/100‑1 (MQ=255)

                          aaccTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTc                                                                           <  2:3344733/100‑1 (MQ=255)

                            ccTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAg                                                                         <  1:3696019/100‑1 (MQ=255)

                            ccTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAg                                                                         <  2:1645467/100‑1 (MQ=255)

                            ccTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAg                                                                         >  1:1615750/1‑100 (MQ=255)

                              tCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGa                                                                       >  1:456998/1‑100 (MQ=255)

                                 cGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGc                                                                    <  1:3050766/100‑1 (MQ=255)

                                  gCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGcc                                                                   <  1:2114076/100‑1 (MQ=255)

                                  gCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGcc                                                                   >  1:2638001/1‑100 (MQ=255)

                                   ccGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCg                                                                  <  2:2629598/100‑1 (MQ=255)

                                   ccGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCg                                                                  >  2:3279027/1‑100 (MQ=255)

                                   ccGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGGTTGCACAACTGTGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCg                                                                  >  2:3011126/1‑100 (MQ=255)

                                    cGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCgg                                                                 <  2:2214981/100‑1 (MQ=255)

                                      gACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGAc                                                               <  1:1014641/100‑1 (MQ=255)

                                       aCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACg                                                              <  1:328021/100‑1 (MQ=255)

                                       aCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACg                                                              <  1:305980/100‑1 (MQ=255)

                                        cAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTGTGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGt                                                             >  1:417214/1‑100 (MQ=255)

                                        cAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTGTGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGt                                                             >  2:1964780/1‑100 (MQ=255)

                                          aaCTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTGTGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTa                                                           >  1:2772161/1‑100 (MQ=255)

                                          aaCTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTa                                                           >  2:3778498/1‑100 (MQ=255)

                                            cTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTGTGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATc                                                         >  1:21949/1‑100 (MQ=255)

                                            cTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTGTGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATc                                                         <  2:1130981/100‑1 (MQ=255)

                                             tGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATCa                                                        <  1:672514/100‑1 (MQ=255)

                                              gggCGTGGTGGCGCTGTACCTGGAGGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCGGAAGTCGAGCGGTCAGGATGCCGGACGTTATCa                                                        <  2:1724702/99‑1 (MQ=255)

                                              gggCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTGTGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATCAg                                                       <  1:141639/100‑1 (MQ=255)

                                              gggCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATCAg                                                       <  1:58155/100‑1 (MQ=255)

                                              gggCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATCAg                                                       >  1:3776043/1‑100 (MQ=255)

                                                gCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTGTGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATCAGCt                                                     >  2:2532734/1‑100 (MQ=255)

                                                 cGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATCAGCTc                                                    <  2:731542/100‑1 (MQ=255)

                                                  gtggtgGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATCAGCTCa                                                   <  1:3561295/100‑1 (MQ=255)

                                                    ggtggCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTGTGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATCAGCTCAAc                                                 >  1:2498547/1‑100 (MQ=255)

                                                     gtggCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATCAGCTCAACa                                                <  2:476738/100‑1 (MQ=255)

                                                      tggCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATCAGCTCAACAt                                               <  1:3428161/100‑1 (MQ=255)

                                                        gcgcTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATCAGCTCAACAtg                                              <  2:1435959/99‑1 (MQ=255)

                                                         cgcTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTGTGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATCAGCTCAACAtgtg                                            >  1:1284665/1‑100 (MQ=255)

                                                           cTGCACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCTGGACGTTATCAGCTCAACAtgtg                                            <  2:3370935/98‑1 (MQ=255)

                                                            tGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTTAGGATGCCGGACGTTATCAGCTCAACATGTGGAg                                         <  1:517243/100‑1 (MQ=255)

                                                            tGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATCAGCTCAACATGTGGAg                                         <  2:2159059/100‑1 (MQ=255)

                                                            tGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATCAGCTCAACATGTGGAg                                         >  2:340711/1‑100 (MQ=255)

                                                               aCCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATCAGCTCAACATGTGGAGCaa                                      >  1:832439/1‑100 (MQ=255)

                                                                cccGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATCAGCTCAACATGTGGAGCaaa                                     >  1:664274/1‑100 (MQ=255)

                                                                cccGGATGCACTCTCTATCAGCGGTTTGCAAAACTGTGTGAAGTATCTGAAGTCGAGCGGTCAGGATGACGGACGTTATCAGCTCAACATCTGGGGCaaa                                     >  2:2367057/1‑100 (MQ=255)

                                                                 ccGGATGCACTCTCTATCAGCGGTTTGCACAACTGCGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATCAGCTCAACATGTGGAGAaaaa                                    <  2:2731577/100‑1 (MQ=255)

                                                                 ccGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATCAGCTCAACATGTGGAGCaaaa                                    <  1:3640666/100‑1 (MQ=255)

                                                                      tGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATCAGCTCAACATGTGGAGCAAAATCTTc                               <  1:1875151/100‑1 (MQ=255)

                                                                        cACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATCAGCTCAACATGTGGAGCAAAATCTTCCa                             >  2:1403669/1‑100 (MQ=255)

                                                                        cACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATCAGCTCAACATGTGGAGCAAAATCTTCCa                             >  1:2700812/1‑100 (MQ=255)

                                                                         aCTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATCAGCTCAACATGTGGAGCAAAATCTTCCa                             <  2:5394/99‑1 (MQ=255)

                                                                         aCTCTCTATAAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATCACGGACGTTATCAGCTCAACATGTGGAGCAAAATCTTCCAg                            >  2:2965331/1‑100 (MQ=255)

                                                                          ctctctAGCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATCAGCTCAACATGTGGAGCAAAATCTTCCAGc                           <  2:1261819/100‑1 (MQ=255)

                                                                           tctctATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATCAGCTCAACATGTGGAGCAAAATCTTCCAGcc                          <  2:2793/100‑1 (MQ=255)

                                                                            ntctATCAGCGGTTTGCACAACTGTGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATCAGCTCAACATGTGGAGCAAAATCTTCCAGCCg                         >  2:3081192/2‑100 (MQ=255)

                                                                             tctATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATCAGCTCAACATGTGGAGCAAAATCTTCCAGCCGc                        >  1:1438296/1‑100 (MQ=255)

                                                                               tATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATCAGCTCAACATGTGGAGCAAAATCTTCCAGCCGCt                       <  2:1756981/99‑1 (MQ=255)

                                                                               tATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATCAGCTCAACATGTGGAGCAAAATCTTCCAGCCGCTa                      >  1:3453265/1‑100 (MQ=255)

                                                                                 tCAGCGGTTTGCACAACTGTGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATCAGCTCAACATGTGGAGCAAAATCTTCCAGCCGCTATc                    <  1:2407021/100‑1 (MQ=255)

                                                                                 tCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATCAGCTCAACATGTGGAGCAAAATCTTCCAGCCGCTATc                    >  2:3339464/1‑100 (MQ=255)

                                                                                      ggTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATCAGCTCAACATGTGGAGCAAAATCTTCCAGCCGCTATCTGTgg               >  1:2798260/1‑100 (MQ=255)

                                                                                         ttGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATCAGCTCAACATGTGGAGCAAAATCTTCCAGCCGCTATCTGTGGCgg            <  2:2640720/100‑1 (MQ=255)

                                                                                           gCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATCAGCTCAACATGTGGAGCAAAATCTTCCAGCCGCTATCTGTGGCGGtg          >  2:2183399/1‑100 (MQ=255)

                                                                                           gCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATCAGCTCAACATGTGGAGCAAAATCTTCCAGCCGCTATCTGTGGCGGtg          >  2:3706623/1‑100 (MQ=255)

                                                                                           gCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATCAGCTCAACATGTGGAGCAAAATCTTCCAGCCGCTATCTGTGGCGGtg          >  2:241873/1‑100 (MQ=255)

                                                                                           gCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATCAGCTCAACATGTGGAGCAAAATCTTCCAGCCGCTATCTGTGGCGGtg          <  2:2543278/100‑1 (MQ=255)

                                                                                            cacaACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATCAGCTCAACATGTGGAGCAAAATCTTCCAGCCGCTATCTGTGGCGGtg          >  2:746001/1‑99 (MQ=255)

                                                                                             acaACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATCAGCTCAACATGTGGAGCAAAATCTTCCAGCCGCTATCTGTGGCGGtgat        <  1:1992653/100‑1 (MQ=255)

                                                                                              caACTGTGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATCAGCTCAACATGTGGAGCAAAATCTTCCAGCCGCTATCTGTGGCGGtgatg       >  2:3705376/1‑100 (MQ=255)

                                                                                              caACTGTGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATCAGCTCAACATGTGGAGCAAAATCTTCCAGCCGCTATCTGTGGCGGtgatg       >  1:816801/1‑100 (MQ=255)

                                                                                              caACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATCAGCTCAACATGTGGAGCAAAATCTTCCAGCCGCTATCTGTGGCGGtgatg       <  1:1840148/100‑1 (MQ=255)

                                                                                              caACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATCAGCTCAACATGTGGAGCAAAATCTTCCAGCCGCTATCTGTGGCGGtgatg       <  1:769245/100‑1 (MQ=255)

                                                                                              caACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATCAGCTCAACATGTGGAGCAAAATCTTCCAGCCGCTATCTGTGGCGGtgatg       <  2:653312/100‑1 (MQ=255)

                                                                                              caACTATGTGAAGTATCTGAAGTCGAGCGGTCAGAATGCCGGACGTTATCAGCTCAACATGTGGAGCAAAATCTTCCAGCCGCTATCTGTGGCGGtga         >  2:1060389/1‑98 (MQ=255)

                                                                                                  tATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATCAGCTCAACATGTGGAGCAAAATCTTCCAGCCGCTATCTGTGGCGGTGATGATGc   <  2:2093403/100‑1 (MQ=255)

                                                                                                   aTGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATCAGCTCAACCTGTGGAGCAAAATCTTCCAGCCGCTATCTGTGGCGGTGATGATGCt  <  2:2110497/100‑1 (MQ=255)

                                                                                                   aTGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATCAGCTCAACATGTGGAGCAAAATCTTCCAGCCGCTATCTGTGGCGGTGATGATGc   >  1:3304146/1‑99 (MQ=255)

AGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGGACGTTATCAGCTCAACATGTGGAGCAAAATCTTCCAGCCGCTATCTGTGGCGGTGATGATGCT  >  NC_000913/4487988‑4488186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A