Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A16 F258 I1 R1	132	116.1	5785507	94.2%	5449947	99.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,499,708	(C)_5→4	intergenic (+93/‑202)	yhfL → / → frlA	small lipoprotein/putative fructoselysine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,499,704	0	C	.	96.5%	481.2 / 8.7	113	intergenic (+89/‑206)	yhfL/frlA	small lipoprotein/putative fructoselysine transporter
Reads supporting (aligned to +/- strand): ref base C (2/2); new base . (45/64); total (47/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

CTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTC  >  NC_000913/3499605‑3499799

cTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGc                                                                                                 >  2:2413706/1‑100 (MQ=255)

 tGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGcc                                                                                                >  1:705415/1‑100 (MQ=255)

 tGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGcc                                                                                                <  2:13650/100‑1 (MQ=255)

  gCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGccc                                                                                               >  2:673872/1‑100 (MQ=255)

    acaGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGccccg                                                                                             <  2:2110536/100‑2 (MQ=255)

    acaGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGccccg                                                                                             <  1:2508199/100‑2 (MQ=255)

       gTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGccccgccc                                                                                          <  1:830022/100‑5 (MQ=255)

          aGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTa                                                                                      >  2:2456992/1‑100 (MQ=255)

          aGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTa                                                                                      >  2:2222046/1‑100 (MQ=255)

           gTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTaa                                                                                     >  1:295896/1‑100 (MQ=255)

           gTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTaa                                                                                     <  1:469656/100‑1 (MQ=255)

             ctctGACAGAAATCGGCTAACACCGGTTTAACTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTaaaa                                                                                   >  2:388732/1‑100 (MQ=255)

              tctGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTaaaaa                                                                                  <  2:206745/100‑1 (MQ=255)

              tctGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTaaaaa                                                                                  <  1:741498/100‑1 (MQ=255)

              tctGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTaaaaa                                                                                  >  1:849156/1‑100 (MQ=255)

                 gACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAatat                                                                               >  2:1609491/1‑100 (MQ=255)

                   cAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATAttt                                                                             >  1:1556131/1‑100 (MQ=255)

                   cAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATAttt                                                                             >  1:1461605/1‑100 (MQ=255)

                     gAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTc                                                                           >  1:579070/1‑100 (MQ=255)

                     gAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTc                                                                           >  1:26165/1‑100 (MQ=255)

                     gAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTc                                                                           >  1:613364/1‑100 (MQ=255)

                     gAAATCGACTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTc                                                                           <  1:2231596/100‑1 (MQ=255)

                      aaaTCGGCTACCACCGGTTTAATTGCCCGGCGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTT‑CCCCCGCCCGTAAAAATATTTTc                                                                           <  2:1685775/99‑1 (MQ=255)

                       aaTCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCAc                                                                         <  2:2695123/100‑1 (MQ=255)

                       aaTCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCAc                                                                         <  1:1324311/100‑1 (MQ=255)

                       aaTCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCAc                                                                         <  1:939364/100‑1 (MQ=255)

                       aaTCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCAc                                                                         <  2:1230415/100‑1 (MQ=255)

                        aTCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACt                                                                        <  1:2473054/100‑1 (MQ=255)

                           ggCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTa                                                                     <  1:710004/100‑1 (MQ=255)

                           ggCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTa                                                                     <  2:1405552/100‑1 (MQ=255)

                             cTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTaaa                                                                   <  2:2170810/100‑1 (MQ=255)

                             cTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTaaa                                                                   >  1:928952/1‑100 (MQ=255)

                              tAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAAt                                                                  <  2:1914423/100‑1 (MQ=255)

                              tAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAAt                                                                  <  1:2567491/100‑1 (MQ=255)

                               aaCACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACttagatc                                                                  >  1:1919637/1‑95 (MQ=255)

                                 cacCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCa                                                               >  1:1642317/1‑100 (MQ=255)

                                  acCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCaa                                                              >  1:11000/1‑100 (MQ=255)

                                   ccGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAAt                                                             <  2:187416/100‑1 (MQ=255)

                                    cGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAAtt                                                            >  2:2234483/1‑100 (MQ=255)

                                      gTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTg                                                          <  2:2808117/100‑1 (MQ=255)

                                      gTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTg                                                          <  2:1795986/100‑1 (MQ=255)

                                      gTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTg                                                          <  1:1974014/100‑1 (MQ=255)

                                       tttAATTGCCCGACGCCCTCTTGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGa                                                         <  2:2576232/100‑1 (MQ=255)

                                        ttAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGAt                                                        <  1:274919/100‑1 (MQ=255)

                                         tAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATa                                                       <  1:1609501/100‑1 (MQ=255)

                                           aTTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATaan                                                     <  2:1472831/100‑2 (MQ=255)

                                           aTTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAAc                                                     >  1:2879156/1‑100 (MQ=255)

                                            ttttccGACCCCCTCTGGATTTTCCGAGGGCGTATTTATTTCTATGTCATTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACt                                                    <  2:1428082/96‑1 (MQ=255)

                                            ttGCCCGACGTCCTCTGGATTTTCCGAGGGCGTATTTTTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACt                                                    <  2:2300535/100‑1 (MQ=255)

                                            ttGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACt                                                    <  2:341810/100‑1 (MQ=255)

                                            ttGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACt                                                    <  1:2828997/100‑1 (MQ=255)

                                             tGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTa                                                   <  2:1637593/100‑1 (MQ=255)

                                              gCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTAc                                                  <  1:27955/100‑1 (MQ=255)

                                               nccGACGCCCTCTGGATTTTCCGAGGGCGTATTAGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATATTCACTTTAAATTCAATTTGATAACTACa                                                 >  2:1738622/2‑100 (MQ=255)

                                               cccGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTAc                                                  >  1:759495/1‑100 (MQ=255)

                                               cccGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACa                                                 >  2:1906320/1‑100 (MQ=255)

                                                 cGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATc                                               >  1:1056650/1‑100 (MQ=255)

                                                      cccTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTa                                          <  1:2298880/100‑1 (MQ=255)

                                                       ccTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAg                                         <  1:1674338/100‑1 (MQ=255)

                                                        ctctGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATGACTACATCCATTAGc                                        >  1:2847083/1‑100 (MQ=255)

                                                        ctctGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGc                                        <  1:2359918/100‑1 (MQ=255)

                                                         tctGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTCCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGcc                                       >  2:1249995/1‑100 (MQ=255)

                                                          ctGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGcc                                       <  2:1355708/99‑1 (MQ=255)

                                                               ttttCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAgcg                                 >  1:1412401/1‑100 (MQ=255)

                                                               ttttCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑ACCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAgcg                                 >  1:2033663/1‑100 (MQ=255)

                                                                   ccGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGAt                             <  2:352852/100‑1 (MQ=255)

                                                                    cGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATc                            >  1:2132196/1‑100 (MQ=255)

                                                                     gAGGGCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATcc                           <  2:634640/100‑1 (MQ=255)

                                                                        ggCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCtgt                        <  2:2476552/100‑1 (MQ=255)

                                                                         gCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTGCATCCATTAGCCACAGCGCGATCCtgtg                       <  2:1623173/100‑1 (MQ=255)

                                                                         gCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATcc                           <  2:1232588/96‑1 (MQ=255)

                                                                         gCGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACAGCCATTAGCCACAGCGCGATCCtgt                        <  1:1315825/99‑1 (MQ=255)

                                                                          cGTATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGc                      <  2:613362/100‑1 (MQ=255)

                                                                            tATTTGTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCg                     >  1:1127829/1‑99 (MQ=255)

                                                                                 gTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAAttt               >  1:1857182/1‑100 (MQ=255)

                                                                                 gTTTCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAAttt               >  1:1107295/1‑100 (MQ=255)

                                                                                   ttCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTtg             <  1:2707090/100‑1 (MQ=255)

                                                                                   ttCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATCTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTtg             <  2:1532328/100‑1 (MQ=255)

                                                                                    tCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTtgt            >  1:136155/1‑100 (MQ=255)

                                                                                    tCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTtgt            <  1:551387/100‑1 (MQ=255)

                                                                                    tCTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTtgt            <  1:2527743/100‑1 (MQ=255)

                                                                                     cTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTtgtg           <  2:1461598/100‑1 (MQ=255)

                                                                                     cTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTtgt            >  2:2236351/1‑99 (MQ=255)

                                                                                     cTATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACACCCATTAGCCACAGCGCGATCCTGTGCGAAATTTtgtg           <  2:1545580/100‑1 (MQ=255)

                                                                                      tATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGa          <  1:1130484/100‑1 (MQ=255)

                                                                                      tATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGa          <  1:318770/100‑1 (MQ=255)

                                                                                      tATGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGa          <  1:571132/100‑1 (MQ=255)

                                                                                        tGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATc        >  2:2110761/1‑100 (MQ=255)

                                                                                        tGTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATc        <  2:1191005/100‑1 (MQ=255)

                                                                                         gTCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCt       >  1:69377/1‑100 (MQ=255)

                                                                                         gTCCTTCTTG‑CCCCGCCCGAAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAACAACAGCGCGATCCTGTGCGAAATTTTGTGATCt       >  2:138552/1‑100 (MQ=255)

                                                                                          tCCTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCtt      >  1:2504369/1‑100 (MQ=255)

                                                                                           ccTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTtc     >  2:1560132/1‑100 (MQ=255)

                                                                                           ccTTCTTG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTtc     >  1:1578644/1‑100 (MQ=255)

                                                                                            cttcttG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTtcc    <  1:909040/100‑1 (MQ=255)

                                                                                              tcttG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCACTTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTtcctc  <  2:2033649/100‑1 (MQ=255)

                                                                                              tcttG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCGTTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTtcctc  >  2:1855520/1‑100 (MQ=255)

                                                                                              tcttG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTtcctc  >  2:543330/1‑100 (MQ=255)

                                                                                              tcttG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTtcctc  >  1:2765813/1‑100 (MQ=255)

                                                                                              tcttG‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTtcctc  >  2:898905/1‑100 (MQ=255)

CTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTC  >  NC_000913/3499605‑3499799

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A