Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F210 I1 R1 75 123.0 6181441 93.9% 5804373 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,436,256 G→A A350A (GCG→GCA)  rsmB → 16S rRNA m(5)C967 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,436,2560GA99.2% 438.0 / NA 128A350A (GCG→GCA) rsmB16S rRNA m(5)C967 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (55/72);  total (56/72)
Fisher's exact test for biased strand distribution p-value = 4.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 128 total aligned reads displayed.

CATTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAA  >  NC_000913/3436160‑3436351
                                                                                                |                                                                                               
cATTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACaa                                                                                              >  2:423310/1‑100 (MQ=255)
  ttttATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAAtt                                                                                            <  1:1284111/100‑1 (MQ=255)
   tttATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTg                                                                                           <  1:492663/100‑1 (MQ=255)
    ttattaGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGc                                                                                          <  2:2968423/100‑1 (MQ=255)
    ttattaGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGc                                                                                          <  1:110910/100‑1 (MQ=255)
      attaGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAg                                                                                        <  2:2760397/100‑1 (MQ=255)
      attaGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAg                                                                                        <  1:2821433/100‑1 (MQ=255)
      attaGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAg                                                                                        <  2:2860347/100‑1 (MQ=255)
      attaGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAg                                                                                        <  1:100080/100‑1 (MQ=255)
        taGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTc                                                                                      <  1:596791/100‑1 (MQ=255)
         aGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCt                                                                                     >  2:779289/1‑100 (MQ=255)
         aGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCt                                                                                     >  1:1499680/1‑100 (MQ=255)
           aTGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGa                                                                                   <  1:2527484/100‑1 (MQ=255)
            tGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGTGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGaa                                                                                  >  1:2318616/1‑100 (MQ=255)
            tGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGaa                                                                                  >  2:270889/1‑100 (MQ=255)
              cgcCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGaact                                                                                >  1:961976/1‑98 (MQ=255)
              cgcCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAAt                                                                                >  1:1142982/1‑100 (MQ=255)
              cgcCTTGTTCAGCAACCGGTGTGATTAGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAAt                                                                                <  2:864115/100‑1 (MQ=255)
                ccTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATtc                                                                              >  1:1084294/1‑100 (MQ=255)
                ccTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATtc                                                                              <  2:1533267/100‑1 (MQ=255)
                 cTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATtct                                                                             <  2:2363961/100‑1 (MQ=255)
                  ttGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATtctc                                                                            <  2:609391/100‑1 (MQ=255)
                   tGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCg                                                                           <  1:3021127/100‑1 (MQ=255)
                     ttCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGCACTCGCACAATTGCAGTCTGAAATTCTCGAc                                                                         >  2:1314000/1‑100 (MQ=255)
                     ttCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGACCTCGCACAATTGCAGTCTGAAATTCTCGAc                                                                         >  2:286903/1‑100 (MQ=255)
                      ncAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACg                                                                        >  1:1165507/2‑100 (MQ=255)
                       cAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGc                                                                       >  1:2541569/1‑100 (MQ=255)
                       cAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGc                                                                       <  2:850419/100‑1 (MQ=255)
                        aGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGc                                                                       >  1:397033/1‑99 (MQ=255)
                          cAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCAt                                                                    >  2:961380/1‑100 (MQ=255)
                           aaCCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCAtt                                                                   <  2:1953274/100‑1 (MQ=255)
                             ccGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTg                                                                 >  2:1133572/1‑100 (MQ=255)
                              cGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTgg                                                                >  1:1788784/1‑100 (MQ=255)
                              cGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTgg                                                                <  2:764979/100‑1 (MQ=255)
                               ggTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGc                                                               >  2:1586487/1‑100 (MQ=255)
                               ggTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGc                                                               <  1:3070835/100‑1 (MQ=255)
                                gtgtGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGcc                                                              >  1:1909301/1‑100 (MQ=255)
                                   tgATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCa                                                           >  1:973873/1‑100 (MQ=255)
                                   tgATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCa                                                           <  2:332294/100‑1 (MQ=255)
                                    gATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGcct                                                          >  2:1767214/1‑98 (MQ=255)
                                    gATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCAt                                                          >  2:967675/1‑100 (MQ=255)
                                    gATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCAt                                                          <  1:556295/100‑1 (MQ=255)
                                    gATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCAt                                                          >  1:1036297/1‑100 (MQ=255)
                                    gATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCAt                                                          >  1:1630426/1‑100 (MQ=255)
                                     aTTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCAtt                                                         <  1:496442/100‑1 (MQ=255)
                                     aTTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCAtt                                                         >  1:2514392/1‑100 (MQ=255)
                                       tcgtcgCCATCCAGATATTAAATGGTTACGTCGCGGTCGCGATATCCCGGAACTCGCACCATTGCAGGCTGAAATTCTCCACGCCCTTTGGCCGCATTTa                                                       >  2:2886596/1‑100 (MQ=255)
                                       tcgtcgCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTa                                                       <  1:1136921/100‑1 (MQ=255)
                                        cgtcgCCATCCAGATATTAAATGGTTACGTCGCTATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCAttt                                                        <  2:2588934/98‑1 (MQ=255)
                                        cgtcgCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTaaa                                                      >  1:2676105/1‑100 (MQ=255)
                                        cgtcgCCATCCAGATATTAAATGGTTACGTCGCGATCACGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTaa                                                      >  2:1651606/1‑100 (MQ=255)
                                         gtcgCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTaaa                                                     <  1:1314011/100‑1 (MQ=255)
                                         gtcgCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTaaa                                                     <  1:2840712/100‑1 (MQ=255)
                                          tcgCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTaaaa                                                    >  2:655412/1‑100 (MQ=255)
                                          tcgCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTaaaa                                                    >  2:1110416/1‑100 (MQ=255)
                                          tcgCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTaaaa                                                    <  1:657271/100‑1 (MQ=255)
                                            gCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAAc                                                  >  1:3080303/1‑100 (MQ=255)
                                             ccATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAAcc                                                 <  1:539551/100‑1 (MQ=255)
                                               aTCCATATACCAAATGATTACGGCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCgg                                               <  2:175003/100‑1 (MQ=255)
                                                tCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGt                                              <  2:1490575/100‑1 (MQ=255)
                                                 ccAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTg                                             <  1:261699/100‑1 (MQ=255)
                                                  cAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTgg                                            <  1:1209135/100‑1 (MQ=255)
                                                  cAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTgg                                            >  1:2134827/1‑100 (MQ=255)
                                                  cAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTgg                                            >  1:1080032/1‑100 (MQ=255)
                                                  cAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTgg                                            >  1:492267/1‑100 (MQ=255)
                                                   aGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGa                                           <  2:1142972/100‑1 (MQ=255)
                                                   aGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGa                                           <  1:2759349/100‑1 (MQ=255)
                                                      tatTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAAct                                        >  2:1455576/1‑100 (MQ=255)
                                                         tAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTg                                     <  2:121772/100‑1 (MQ=255)
                                                          aaaTGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTgg                                    <  2:309297/100‑1 (MQ=255)
                                                          aaaTGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATGCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTgg                                    <  2:1296692/100‑1 (MQ=255)
                                                           aaTGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGt                                   <  1:2150417/100‑1 (MQ=255)
                                                               gTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTAt                               >  1:256463/1‑100 (MQ=255)
                                                                ttACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATg                              <  1:423312/100‑1 (MQ=255)
                                                                ttACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATg                              <  2:1981709/100‑1 (MQ=255)
                                                                ttACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATg                              <  1:1108830/100‑1 (MQ=255)
                                                                ttACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATg                              <  2:457539/100‑1 (MQ=255)
                                                                  aCGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGcc                            >  1:167298/1‑100 (MQ=255)
                                                                  aCGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGcc                            <  1:113128/100‑1 (MQ=255)
                                                                   cGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCa                           >  2:1764479/1‑100 (MQ=255)
                                                                     tcgcgatcgcgaTATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCAcc                         <  1:970633/100‑1 (MQ=255)
                                                                     tcgcgatcgcgaTATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCAcc                         <  1:405870/100‑1 (MQ=255)
                                                                        cgatcgcgatATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGt                      >  2:1849664/1‑100 (MQ=255)
                                                                            ccgatcttCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCgg                  <  1:2869398/93‑1 (MQ=255)
                                                                             gcgatATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGgt                 <  2:1630408/100‑1 (MQ=255)
                                                                             gcgatATCCCGGAACTAGAACAATTGCAGTCTGAAATTCTAGACGCCATTTGGCCGGATTTAAAACCCGGTGGACCTCTGGTCTATGCCACCTGTTCgg                  >  2:616879/1‑99 (MQ=255)
                                                                              cgatATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGgtg                <  2:203740/100‑1 (MQ=255)
                                                                              cgatATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGgtg                <  2:1620577/100‑1 (MQ=255)
                                                                               gatATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGgtgt               <  2:3000099/100‑1 (MQ=255)
                                                                                  atCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTa             <  2:961965/99‑1 (MQ=255)
                                                                                    cccGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCg          >  2:116153/1‑100 (MQ=255)
                                                                                    cccGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCg          <  1:1533614/100‑1 (MQ=255)
                                                                                    cccGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCg          >  1:3014675/1‑100 (MQ=255)
                                                                                     ccGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCgg         <  2:2812541/100‑1 (MQ=255)
                                                                                     ccGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCgg         <  1:2269533/100‑1 (MQ=255)
                                                                                         aaCTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAaga     <  1:967686/100‑1 (MQ=255)
                                                                                          aCTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAagag    <  1:2371940/100‑1 (MQ=255)
                                                                                           cTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAgaga   <  1:1945386/100‑1 (MQ=255)
                                                                                           cTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAgaga   >  1:2603680/1‑100 (MQ=255)
                                                                                            tCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGaa  <  2:2318593/100‑1 (MQ=255)
                                                                                                |                                                                                               
CATTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAA  >  NC_000913/3436160‑3436351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A