Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F125 I0 R1 65 108.6 5319566 96.1% 5112102 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,595,315 (A)5→4 100% coding (166/1278 nt) livM ← branched‑chain amino acid ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,595,3110A.60.0% 46.2 / 175.9 90coding (170/1278 nt)livMbranched‑chain amino acid ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (22/14);  new base . (24/30);  total (46/44)
Fisher's exact test for biased strand distribution p-value = 1.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01

Only 100 of 101 total aligned reads displayed.

CTGCTTCACCGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCACGCCC  >  NC_000913/3595214‑3595409
                                                                                                 |                                                                                                  
cTGCTTCACCGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGaaa                                                                                                  >  2:699464/1‑100 (MQ=255)
  gCTTCACCGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGaaaaa                                                                                                >  1:593956/1‑100 (MQ=255)
  gCTTCACCGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGaaaa                                                                                                 >  2:180303/1‑99 (MQ=255)
  gCTTCACCGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCCGGAAGaaaag                                                                                                <  1:2011014/100‑2 (MQ=255)
  gCTTCACCGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTATGGAAAGCCGGTCGCAAAAGCTGGAAGaaaag                                                                                                >  1:2044972/1‑99 (MQ=255)
  gCTTCACCGTGGAGCCATCAAGAACAGGCAGAACAAACTGCGGTCCGGAAAAGCTTTTGAACCCTTTCTGGAAAGACGGTCGCAAAAGCTGGACGaaaag                                                                                                >  2:1940124/1‑99 (MQ=255)
      cACCGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGaaaagacc                                                                                             <  2:717263/99‑5 (MQ=255)
        ccGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAAGaccac                                                                                          >  2:416799/1‑100 (MQ=255)
          tgcccgCCATTGATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCAGGTCGCAAAAGCTGGAAG‑AAAAGACCAccgc                                                                                       >  1:1270534/6‑100 (MQ=255)
          gTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAAGACCAccg                                                                                        >  2:1331403/1‑100 (MQ=255)
          gTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAAGACCAccg                                                                                        >  1:1449685/1‑100 (MQ=255)
              aGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAAGACCACCGCCGt                                                                                    >  1:461079/1‑100 (MQ=255)
               gCCATCAATGGCGGGCATAATAAACTTCGGTCCGGAATCTCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGc                                                                                  <  1:751481/100‑1 (MQ=255)
                ccATCAATGGCGGGCAGAATAAACTTCGTTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGcc                                                                                 <  1:513554/100‑1 (MQ=255)
                ccATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGcc                                                                                 <  2:2570889/100‑1 (MQ=255)
                ccATCAATGGCGGGCAGAATAAACTTCAGTCCGGAAACGCTTTTCAACCCTTTATGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGcc                                                                                 <  2:2625263/100‑1 (MQ=255)
                 cATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAAGACCACCGCCGTGcc                                                                                 >  2:10947/1‑100 (MQ=255)
                 cATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAAGACCACCGCCGTGcc                                                                                 <  2:1108616/100‑1 (MQ=255)
                  aTCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGa                                                                               <  1:589360/100‑1 (MQ=255)
                  aTCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGa                                                                               <  2:1153641/100‑1 (MQ=255)
                   tCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACGACCGCCGTGCCGa                                                                               >  2:528094/1‑99 (MQ=255)
                   tCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGAt                                                                              <  1:1569801/100‑1 (MQ=255)
                      aTGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATaaa                                                                           >  1:1843387/1‑100 (MQ=255)
                      aTGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATaaa                                                                           <  1:1957902/100‑1 (MQ=255)
                      aTGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATaaa                                                                           <  1:1605189/100‑1 (MQ=255)
                        ggCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAaca                                                                         <  1:1589982/100‑1 (MQ=255)
                            ggCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAAGACCACCGCCGTGCCGATAAACAccc                                                                      >  1:1759193/1‑100 (MQ=255)
                             gCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGTCGGTCGCAAAAGCTGGACG‑AAAAGACCACCGCCGTGCCGATAAacacacac                                                                    >  2:2164423/1‑100 (MQ=255)
                               aGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTg                                                                  <  2:2609796/100‑1 (MQ=255)
                                gAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGc                                                                 <  2:1580910/100‑1 (MQ=255)
                                gAATAAACTTCGGTCCGGAAACGCTTTCCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAAGACCACCGCCGTGCCGATAAACACCCACTg                                                                  >  2:1302953/1‑100 (MQ=255)
                                   tAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCa                                                               <  1:2172783/100‑1 (MQ=255)
                                   tAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCaa                                                              >  2:2523904/1‑100 (MQ=255)
                                   tAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCaa                                                              >  1:766495/1‑100 (MQ=255)
                                    aaaCTTCGGTCCGAAAACGCTTTTCAACCCTATCTGGAAAGCCGGTCGCAAAAGATGGAAGAAAAAGACCACCGTCGTTCCGATCAACACCCAATGCCaa                                                              >  2:296833/1‑100 (MQ=255)
                                     aaCTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACg                                                            >  2:2377128/1‑100 (MQ=255)
                                      aCTTCGTTCCGGAAACGCTTTTGAACCCTTTCTGGAAAGCCGGTAGCGAAAGCTGGAAGAAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAAc                                                             >  2:1612479/1‑99 (MQ=255)
                                      aCTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACg                                                            <  1:1452698/100‑1 (MQ=255)
                                      aCTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACgg                                                           <  2:637302/100‑1 (MQ=255)
                                      aCTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACgg                                                           <  1:689978/100‑1 (MQ=255)
                                      aCTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACg                                                            <  2:1197661/99‑1 (MQ=255)
                                      aCTCCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCAGATCAAAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACgg                                                           <  2:248579/100‑1 (MQ=255)
                                       cTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACgg                                                           <  1:230908/100‑1 (MQ=255)
                                       cTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACgg                                                           <  2:267764/100‑1 (MQ=255)
                                       cTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACgg                                                           <  2:1758247/100‑1 (MQ=255)
                                       cTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAAAgg                                                           <  2:866918/100‑1 (MQ=255)
                                       cTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGa                                                          >  2:2414469/1‑100 (MQ=255)
                                       cTTCGGCCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGa                                                          <  2:1889231/100‑1 (MQ=255)
                                        ttCGGTCCGGAAACGCTTTTCAATCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGa                                                          <  1:1197354/100‑1 (MQ=255)
                                            gTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATcc                                                     >  1:609596/1‑100 (MQ=255)
                                              ccGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCg                                                    >  1:1279869/1‑100 (MQ=255)
                                              ccGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAAGACAACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCg                                                    >  2:2521500/1‑100 (MQ=255)
                                              ccGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGa                                                   >  2:1128412/1‑100 (MQ=255)
                                                ggAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGaa                                                  >  2:921409/1‑100 (MQ=255)
                                                  aaaCGCTTTTGAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAg                                                 >  2:387564/1‑99 (MQ=255)
                                                  aaaCGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAAAACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGc                                                >  1:1079619/1‑100 (MQ=255)
                                                   aaCGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCg                                              <  1:185290/100‑1 (MQ=255)
                                                       cTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTc                                          <  1:687058/100‑1 (MQ=255)
                                                         tttCAACCCTTTCTGGAAAGCCGGTCGCAAGAGGTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCAAACGGACATCCGAGGCCGTGTCGa                                        >  2:1739102/1‑100 (MQ=255)
                                                         tttCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCg                                         >  2:921371/1‑100 (MQ=255)
                                                            cAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGacca                                     >  2:1513938/1‑100 (MQ=255)
                                                            cAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGacca                                     >  1:1490074/1‑100 (MQ=255)
                                                             aaCCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGaccac                                    <  2:2141989/100‑1 (MQ=255)
                                                             aaCCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGacca                                     >  1:2553881/1‑99 (MQ=255)
                                                              aCCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGaccacc                                   <  2:1232427/100‑1 (MQ=255)
                                                               cccTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGAccacca                                  >  1:2596652/1‑100 (MQ=255)
                                                                    tCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGttt                              <  1:1616268/100‑1 (MQ=255)
                                                                    tCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTg                             >  1:1103679/1‑100 (MQ=255)
                                                                       ggAAAGCCGGTCGCAAAAGCTGGAAGAAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGt                           >  1:1136820/1‑100 (MQ=255)
                                                                       ggAAAGCCGGTCGCAAAAGCTGGAAGAAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGt                           >  2:471800/1‑100 (MQ=255)
                                                                        gAAAGCCGGTCGCAAAAGCTGGAAGAAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTg                          <  2:1759176/100‑1 (MQ=255)
                                                                         aaaGCCGGTCGCAAAAGCTGGAAGAAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGc                         <  2:461075/100‑1 (MQ=255)
                                                                          aaGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCa                       <  2:2528188/100‑1 (MQ=255)
                                                                            gCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATc                     >  1:1466177/1‑100 (MQ=255)
                                                                             ccGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATcc                    >  1:317716/1‑100 (MQ=255)
                                                                                gTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGc                 <  1:1065342/100‑1 (MQ=255)
                                                                                 tCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCt                <  1:1037346/100‑1 (MQ=255)
                                                                                 tCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCt                >  2:1321244/1‑100 (MQ=255)
                                                                                  cGCAAAAGCTGGAAGAAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCt                >  2:1350263/1‑100 (MQ=255)
                                                                                    cAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCCCCa             >  2:1844380/1‑100 (MQ=255)
                                                                                    cAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTAGACAAACAGTTTGGTGCCATCCAGCTCCa             >  1:605671/1‑100 (MQ=255)
                                                                                     aaaaGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTct              <  2:1991426/98‑2 (MQ=255)
                                                                                        aGCTGGAAGAAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGtt          <  1:2256999/100‑1 (MQ=255)
                                                                                        aGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACTCCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTg         <  1:833251/100‑1 (MQ=255)
                                                                                        aGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTg         >  1:683698/1‑100 (MQ=255)
                                                                                        aGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTg         <  1:1482587/100‑1 (MQ=255)
                                                                                         gCTGGAAGAAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGAACATCCGAAGCCGAATCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTg         >  1:220177/1‑100 (MQ=255)
                                                                                         gCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGc        <  1:729462/100‑1 (MQ=255)
                                                                                          cTGGAAGAAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGc        <  1:921380/100‑1 (MQ=255)
                                                                                          cTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTgaa       >  2:592693/1‑98 (MQ=255)
                                                                                           tGGAAGAAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCa       >  1:1272768/1‑100 (MQ=255)
                                                                                           tGGAAGAAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCa       >  1:2270615/1‑100 (MQ=255)
                                                                                           tGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCAc      >  1:236973/1‑100 (MQ=255)
                                                                                            ggAAGAAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCAc      >  2:2269567/1‑100 (MQ=255)
                                                                                            ggAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTTCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCACg     <  1:664179/100‑1 (MQ=255)
                                                                                            ggAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCACg     <  2:1103669/100‑1 (MQ=255)
                                                                                              aagaaAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCACGc    >  2:1014960/1‑100 (MQ=255)
                                                                                               agaaAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCACGcc   <  1:387566/100‑1 (MQ=255)
                                                                                                gaaAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCACGccc  >  1:1887785/1‑100 (MQ=255)
                                                                                                 aaaaaGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCTGTTTGGTGCCATCCAGCTCCAGTTGCACGccc  <  1:2521530/99‑1 (MQ=255)
                                                                                                 |                                                                                                  
CTGCTTCACCGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCACGCCC  >  NC_000913/3595214‑3595409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A