Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F53 I1 R1 64 112.2 5657101 94.0% 5317674 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,595,315 (A)5→4 coding (166/1278 nt) livM ← branched‑chain amino acid ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,595,3110A.95.5% 465.1 / 14.8 110coding (170/1278 nt)livMbranched‑chain amino acid ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (3/2);  new base . (48/57);  total (51/59)
Fisher's exact test for biased strand distribution p-value = 6.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 118 total aligned reads displayed.

TTCTGCTTCACCGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCAC  >  NC_000913/3595212‑3595405
                                                                                                   |                                                                                              
ttCTGCTTCACCGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGgaaga                                                                                                >  2:1871427/1‑100 (MQ=255)
   tGCTTCACCGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGaaaa                                                                                             >  2:1815910/1‑100 (MQ=255)
    gCTTCACCGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGaaaag                                                                                            >  2:2607890/1‑99 (MQ=255)
    gCTTCACCGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGaaaag                                                                                            <  2:2706663/100‑2 (MQ=255)
      ttCACCGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGaaaagac                                                                                          >  1:509462/1‑97 (MQ=255)
       tCACCGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGaaaagacc                                                                                         <  1:588067/100‑5 (MQ=255)
          ccGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGaccacc                                                                                     >  2:1220161/1‑100 (MQ=255)
          ccGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGaccacc                                                                                     >  1:52380/1‑100 (MQ=255)
            gTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCAccgc                                                                                   <  1:480743/100‑1 (MQ=255)
                  ccATCAATGGCGGGCAGAATAATCTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGcc                                                                             <  1:1184320/100‑1 (MQ=255)
                  ccATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGcc                                                                             <  1:1014759/100‑1 (MQ=255)
                  ccATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGcc                                                                             <  1:180555/100‑1 (MQ=255)
                   cATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCg                                                                            >  1:2478303/1‑100 (MQ=255)
                   cATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCg                                                                            >  2:2018793/1‑100 (MQ=255)
                   cATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCg                                                                            <  1:1889572/100‑1 (MQ=255)
                   cATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCg                                                                            >  1:1241076/1‑100 (MQ=255)
                   cATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCg                                                                            <  2:2371956/100‑1 (MQ=255)
                   cATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGA‑CACCGCCGTGCCGa                                                                           >  1:1241067/1‑100 (MQ=255)
                    aTCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGa                                                                           >  1:1291319/1‑100 (MQ=255)
                     tCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGAt                                                                          <  1:410330/100‑1 (MQ=255)
                      cAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATa                                                                         <  1:2820430/100‑1 (MQ=255)
                        aTGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATaaa                                                                       >  2:2625399/1‑100 (MQ=255)
                         tGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAac                                                                      <  1:1269315/100‑1 (MQ=255)
                            cGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACGCTTTCTGGAAAGCCGGTCGGAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACAcc                                                                   <  1:1545898/100‑1 (MQ=255)
                            cGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACAcc                                                                   >  1:2046796/1‑100 (MQ=255)
                            cGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACAcc                                                                   >  2:148474/1‑100 (MQ=255)
                             gggCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACAccc                                                                  <  2:491151/100‑1 (MQ=255)
                                 aGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTg                                                              <  2:2328598/100‑1 (MQ=255)
                                  gAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGc                                                             <  2:2412321/100‑1 (MQ=255)
                                   aaTAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGcn                                                            <  2:602708/100‑2 (MQ=255)
                                   aaTAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGcc                                                            >  2:109625/1‑100 (MQ=255)
                                    aTAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCa                                                           <  1:1577813/100‑1 (MQ=255)
                                     tAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCaa                                                          >  2:143106/1‑100 (MQ=255)
                                      aaaCTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAAc                                                         <  2:1295716/100‑1 (MQ=255)
                                      aaaCTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAAc                                                         <  2:2449966/100‑1 (MQ=255)
                                       aaCTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACg                                                        >  1:2724919/1‑100 (MQ=255)
                                       aaCTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACg                                                        >  1:2602885/1‑100 (MQ=255)
                                         cTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGa                                                      >  2:1322456/1‑100 (MQ=255)
                                         cTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGa                                                      <  2:2015081/100‑1 (MQ=255)
                                         cTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGa                                                      >  2:2614421/1‑100 (MQ=255)
                                          ttCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGAc                                                     <  2:167516/100‑1 (MQ=255)
                                             ggTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCACCGCACAt                                                   >  2:549287/1‑99 (MQ=255)
                                              gTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATc                                                  <  2:2505760/100‑1 (MQ=255)
                                              gTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATcc                                                 <  1:1290092/100‑1 (MQ=255)
                                              gTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATc                                                  <  2:1077993/99‑1 (MQ=255)
                                                  ggAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAg                                             <  2:1225636/100‑1 (MQ=255)
                                                   gAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGc                                            >  2:2338930/1‑100 (MQ=255)
                                                    aaaCGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGcc                                           <  2:610511/100‑1 (MQ=255)
                                                        gCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCgtgt                                       <  1:2607869/100‑1 (MQ=255)
                                                         cTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTc                                      <  2:2328173/100‑1 (MQ=255)
                                                         cTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTc                                      <  1:109624/100‑1 (MQ=255)
                                                         cTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTc                                      <  2:1032498/100‑1 (MQ=255)
                                                         cTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTc                                      >  1:1500547/1‑100 (MQ=255)
                                                          ttttCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCg                                     >  1:374348/1‑100 (MQ=255)
                                                          ttttCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCg                                     <  1:423578/100‑1 (MQ=255)
                                                             tCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGCCCACCGCCGTGCCGATAAACACACACTGCGAACGGACATCCGAAGCCGTATCGacc                                  >  1:1946496/1‑100 (MQ=255)
                                                             tCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGacc                                  <  1:2164542/100‑1 (MQ=255)
                                                             tCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGacc                                  <  1:2821262/100‑1 (MQ=255)
                                                             tCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGacc                                  >  2:1707603/1‑100 (MQ=255)
                                                             tCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGacc                                  <  2:273166/100‑1 (MQ=255)
                                                              cAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGacca                                 <  2:52381/100‑1 (MQ=255)
                                                              cAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGacca                                 >  1:1250293/1‑100 (MQ=255)
                                                              cAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGacca                                 >  1:519854/1‑100 (MQ=255)
                                                               aaCCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGaccac                                >  2:95056/1‑100 (MQ=255)
                                                                tcccTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGaccacc                               <  2:2087708/99‑1 (MQ=255)
                                                                 cccTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGAccacca                              >  1:2087693/1‑100 (MQ=255)
                                                                  ccTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAg                             <  2:435761/100‑1 (MQ=255)
                                                                  ccTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAg                             >  2:1830133/1‑100 (MQ=255)
                                                                  ccTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAg                             >  1:1921964/1‑100 (MQ=255)
                                                                   cTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGt                            >  1:1425298/1‑100 (MQ=255)
                                                                       cTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACCCCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTgg                        <  2:2678008/100‑1 (MQ=255)
                                                                       cTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTgg                        >  2:2009832/1‑100 (MQ=255)
                                                                        tGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATATACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGt                       >  1:217635/1‑100 (MQ=255)
                                                                        tGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGt                       >  1:89289/1‑100 (MQ=255)
                                                                         ggAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTg                      <  1:2354543/100‑1 (MQ=255)
                                                                         ggAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTg                      <  1:2079879/100‑1 (MQ=255)
                                                                              gCCGGTCGCCCAAGCTGGAAG‑CAACCACCACCGCCGTGCCGCTCACCACCCCATGCCAACGGCCGAACGAAGACGTGTCGATCACCCGTTAGGTGcta                   <  1:1648248/98‑3 (MQ=255)
                                                                               ccGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATcc                >  1:2558388/1‑100 (MQ=255)
                                                                                  gTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGc             <  2:1152031/100‑1 (MQ=255)
                                                                                   tCGCAAAAGCTGGAAGAAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGc             <  1:1690741/100‑1 (MQ=255)
                                                                                    cGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTc           <  2:2350566/100‑1 (MQ=255)
                                                                                     gCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTcc          >  2:2280502/1‑100 (MQ=255)
                                                                                     gCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTcc          <  2:886238/100‑1 (MQ=255)
                                                                                     gCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTcc          <  2:1781721/100‑1 (MQ=255)
                                                                                     gCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTcc          <  2:1338803/100‑1 (MQ=255)
                                                                                     gCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTcc          >  1:663456/1‑100 (MQ=255)
                                                                                      cAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCa         >  1:226325/1‑100 (MQ=255)
                                                                                      cAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCa         >  1:2170146/1‑100 (MQ=255)
                                                                                       aaaaGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAg        >  2:342078/1‑100 (MQ=255)
                                                                                        aaaGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGt       >  2:150154/1‑100 (MQ=255)
                                                                                        aaaGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGt       <  1:1440775/100‑1 (MQ=255)
                                                                                         aaGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGtt      <  1:830693/100‑1 (MQ=255)
                                                                                          aGCTGGAAGAAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGtt      >  1:2443553/1‑100 (MQ=255)
                                                                                          aGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTg     <  2:2724943/100‑1 (MQ=255)
                                                                                          aGCTGGAAG‑AAAAGA‑CACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGc    <  2:1241075/100‑1 (MQ=255)
                                                                                           gCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTg     <  1:990516/99‑1 (MQ=255)
                                                                                           gCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGc    <  2:519859/100‑1 (MQ=255)
                                                                                            cTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCa   >  2:463123/1‑100 (MQ=255)
                                                                                            cTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCa   >  1:721978/1‑100 (MQ=255)
                                                                                             tGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCAc  <  2:176532/100‑1 (MQ=255)
                                                                                                   |                                                                                              
TTCTGCTTCACCGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCAC  >  NC_000913/3595212‑3595405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A