Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F191 I0 R1	105	142.7	6924153	96.8%	6702580	99.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,903,585	G→A	53.1%	G39G (GGC→GGT)	bglB ←	cryptic phospho‑beta‑glucosidase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,903,585	0	G	A	53.1%	12.0 / 173.9	126	G39G (GGC→GGT)	bglB	cryptic phospho‑beta‑glucosidase B
Reads supporting (aligned to +/- strand): ref base G (30/29); new base A (29/38); total (59/67)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.07e-01

Only 100 of 126 total aligned reads displayed.

TAACGCGATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAA  >  NC_000913/3903489‑3903679

tAACGCGATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTGCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCa                                                                                             >  1:2328260/1‑100 (MQ=255)

 aaCGCGATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCa                                                                                             >  1:2710258/1‑99 (MQ=255)

    gcgATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAg                                                                                         <  1:1111215/100‑1 (MQ=255)

    gcgATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAg                                                                                         >  2:2462089/1‑100 (MQ=255)

      gATATCTTCCGGGTAACGTTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGc                                                                                       <  1:603038/100‑1 (MQ=255)

      gATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGc                                                                                       <  2:2362130/100‑1 (MQ=255)

      gATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGc                                                                                       >  1:934155/1‑100 (MQ=255)

      gATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGc                                                                                       <  1:924964/100‑1 (MQ=255)

      gATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGc                                                                                       <  1:1066814/100‑1 (MQ=255)

      gATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGc                                                                                       <  1:2554720/100‑1 (MQ=255)

      gATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTAAACCATGAGGc                                                                                       >  1:2274000/1‑100 (MQ=255)

         atCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTg                                                                                     >  1:1429874/1‑99 (MQ=255)

          tCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTa                                                                                   <  1:2564129/100‑1 (MQ=255)

            ttCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTaaa                                                                                 >  2:1852414/1‑100 (MQ=255)

                 ggTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCACATTCCGCCATGAGGCTGTAAATCTg                                                                             >  1:915812/1‑99 (MQ=255)

                  gTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAg                                                                           >  2:2178461/1‑100 (MQ=255)

                  gTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAg                                                                           <  1:3229530/100‑1 (MQ=255)

                   tAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAgg                                                                          <  1:817182/100‑1 (MQ=255)

                   tAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAgg                                                                          <  2:449885/100‑1 (MQ=255)

                    aaCGGTGATAACAATCGATGGCGACATCTTTGATATTCTCTTTCCCAAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAgct                                                                         >  1:1244524/1‑98 (MQ=255)

                      cGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCg                                                                       <  1:3177987/100‑1 (MQ=255)

                      cGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAg                                                                           <  1:2654580/96‑1 (MQ=255)

                      cGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTc                                                                        >  1:353308/1‑99 (MQ=255)

                      cGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCg                                                                       <  1:1642418/100‑1 (MQ=255)

                       ggTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCga                                                                      >  2:2055861/1‑100 (MQ=255)

                        gTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCgag                                                                     <  2:2530254/100‑1 (MQ=255)

                            tAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATcc                                                                 >  1:1633526/1‑100 (MQ=255)

                            tAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATcc                                                                 >  2:815670/1‑100 (MQ=255)

                            tAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATcc                                                                 >  1:1602976/1‑100 (MQ=255)

                            tAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATcc                                                                 <  1:3167616/100‑1 (MQ=255)

                            tAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGAGGCGCGGTGCCATTGTTCCCATTACACCATGAGGCTGTAAATCTGAGGGCGAGATcc                                                                 >  2:1023431/1‑100 (MQ=255)

                             aaaaaTCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATccc                                                                <  2:3179905/100‑1 (MQ=255)

                              aaaaTCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCt                                                               >  1:3437202/1‑100 (MQ=255)

                              aaaaTCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCt                                                               >  1:3316370/1‑100 (MQ=255)

                               aaaTCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCtt                                                              >  1:1381224/1‑100 (MQ=255)

                                 aTCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTg                                                            <  2:618127/100‑1 (MQ=255)

                                  tCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTATATCTGAGGTCGAGATCCCTTTGc                                                           <  2:2932365/100‑1 (MQ=255)

                                   cGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGcc                                                          <  2:1602991/100‑1 (MQ=255)

                                    gATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTCAGGTCGAGATCCCTTTGCCa                                                         <  1:2116304/100‑1 (MQ=255)

                                    gATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCa                                                         <  1:3340728/100‑1 (MQ=255)

                                    gATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCa                                                         <  2:2035494/100‑1 (MQ=255)

                                     aTGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCAt                                                        >  1:2603829/1‑100 (MQ=255)

                                      tGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATc                                                       <  2:2710279/100‑1 (MQ=255)

                                       ggCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCt                                                      <  1:538487/100‑1 (MQ=255)

                                         cGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTc                                                    >  1:3122831/1‑100 (MQ=255)

                                         cGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTc                                                    >  2:521768/1‑100 (MQ=255)

                                          gACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATCTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTcc                                                   <  1:2423513/100‑1 (MQ=255)

                                            cATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTg                                                 <  1:3281444/100‑1 (MQ=255)

                                             aTCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAGATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGc                                                >  1:637622/1‑100 (MQ=255)

                                             aTCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTg                                                 <  2:1539289/99‑1 (MQ=255)

                                              tCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGcc                                               <  1:1177109/100‑1 (MQ=255)

                                              tCTTTGATATTCACTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGcc                                               >  2:2277929/1‑100 (MQ=255)

                                               cTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCa                                              >  2:578783/1‑100 (MQ=255)

                                               cTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCa                                              <  1:1657763/100‑1 (MQ=255)

                                               cTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCa                                              <  2:3247443/100‑1 (MQ=255)

                                               cTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCa                                              >  1:275291/1‑100 (MQ=255)

                                                tttGATATTCTCTTTCCCCAGGATGTGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAg                                             <  2:3096594/100‑1 (MQ=255)

                                                tttGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCan                                             <  1:158201/100‑2 (MQ=255)

                                                  tGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGc                                           >  1:1482405/1‑100 (MQ=255)

                                                   gATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCa                                          >  1:809859/1‑100 (MQ=255)

                                                   gATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCa                                          <  2:1721980/100‑1 (MQ=255)

                                                    atatTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCAc                                         >  1:279040/1‑100 (MQ=255)

                                                      atTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCt                                       >  1:1162136/1‑100 (MQ=255)

                                                      atTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCt                                       >  1:690362/1‑100 (MQ=255)

                                                        tctcTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTc                                     >  2:1208259/1‑100 (MQ=255)

                                                        tctcTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTc                                     <  2:706029/100‑1 (MQ=255)

                                                         ctctTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTcc                                    >  1:1229137/1‑100 (MQ=255)

                                                         ctctTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTTCCAGGCACCtttc                                    <  2:2328278/100‑3 (MQ=255)

                                                           ctTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCAc                                  <  2:1837145/100‑1 (MQ=255)

                                                            tttCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCAcc                                 >  2:1291281/1‑100 (MQ=255)

                                                            tttCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCAcc                                 >  2:1205788/1‑100 (MQ=255)

                                                            tttCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCAcc                                 <  2:46630/100‑1 (MQ=255)

                                                            tttCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCAcc                                 <  2:3431978/100‑1 (MQ=255)

                                                             ttCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCt                                <  2:2879351/100‑1 (MQ=255)

                                                               ccccAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGa                              >  1:1423563/1‑100 (MQ=255)

                                                               ccccAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGa                              >  2:1396437/1‑100 (MQ=255)

                                                                cccAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGAt                             <  2:3437230/100‑1 (MQ=255)

                                                                    ggATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTgg                          <  1:3460950/99‑1 (MQ=255)

                                                                     gATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCa                        <  2:854786/100‑1 (MQ=255)

                                                                      aTGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAg                       <  2:1651608/100‑1 (MQ=255)

                                                                       tGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGc                      <  2:334757/100‑1 (MQ=255)

                                                                        gcgcGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCt                     <  2:1429886/100‑1 (MQ=255)

                                                                         cgcgGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTg                    >  2:1719576/1‑100 (MQ=255)

                                                                         cgcgGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTg                    >  2:2604679/1‑100 (MQ=255)

                                                                         cgcgGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTg                    >  1:342641/1‑100 (MQ=255)

                                                                         cgcgGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTg                    >  1:3305309/1‑100 (MQ=255)

                                                                          gcgGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGt                   >  1:1999619/1‑100 (MQ=255)

                                                                           cgGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGtt                  >  2:3352552/1‑100 (MQ=255)

                                                                             gTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTgc                <  1:1136369/100‑1 (MQ=255)

                                                                                ccATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGcc             >  1:2461496/1‑100 (MQ=255)

                                                                                ccATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGcc             <  2:660585/100‑1 (MQ=255)

                                                                                ccATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGcc             <  2:525149/100‑1 (MQ=255)

                                                                                 cATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCa            >  2:1124082/1‑100 (MQ=255)

                                                                                   tttttCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCAcc          <  2:3443552/100‑1 (MQ=255)

                                                                                    ttttCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCAccc         >  2:576275/1‑100 (MQ=255)

                                                                                     tttCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCAcccc        <  2:3429998/100‑1 (MQ=255)

                                                                                     tttCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCAcccc        <  1:2090186/100‑1 (MQ=255)

                                                                                        cccATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGGACCTTCCACCTTATTGGCAGCTGTTGCGCCCCCCCaaa     >  1:794780/1‑100 (MQ=255)

                                                                                          cATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGa   >  1:1136621/1‑100 (MQ=255)

                                                                                           aTTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGaa  >  2:1548894/1‑100 (MQ=255)

TAACGCGATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAA  >  NC_000913/3903489‑3903679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A