Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F244 I0 R1 270 114.4 5840133 93.3% 5448844 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,903,585 G→A 80.9% G39G (GGC→GGT)  bglB ← cryptic phospho‑beta‑glucosidase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,903,5850GA80.9% 276.6 / 50.9 138G39G (GGC→GGT) bglBcryptic phospho‑beta‑glucosidase B
Reads supporting (aligned to +/- strand):  ref base G (12/14);  new base A (50/59);  total (63/75)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 138 total aligned reads displayed.

TAACGCGATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGT  >  NC_000913/3903489‑3903683
                                                                                                 |                                                                                                  
tAACGCGATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCa                                                                                                 <  1:897766/100‑1 (MQ=255)
  actcgATACCTTCCGTGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATg                                                                                               <  1:2853401/97‑1 (MQ=255)
  aCGCGATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATg                                                                                               >  2:247247/1‑100 (MQ=255)
   cgcgATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATg                                                                                               <  1:804365/99‑1 (MQ=255)
   cgcgATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATg                                                                                               >  1:2833708/1‑99 (MQ=255)
   cgcgATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGa                                                                                              >  2:1230890/1‑100 (MQ=255)
   cgcgATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCACCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGa                                                                                              >  1:1007523/1‑100 (MQ=255)
    gcgATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGAg                                                                                             <  2:770232/100‑1 (MQ=255)
      gATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGAGGc                                                                                           <  2:892854/100‑1 (MQ=255)
        tatCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTg                                                                                         <  1:259499/100‑1 (MQ=255)
         atCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGt                                                                                        <  2:1855725/100‑1 (MQ=255)
          tCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTa                                                                                       >  2:1069100/1‑100 (MQ=255)
           cTTCCGGGTAACGTTGATAAAAATCTATGGCGATATCTTTGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTa                                                                                       <  1:200617/99‑1 (MQ=255)
           cTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTaa                                                                                      <  1:533381/100‑1 (MQ=255)
            ttCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTaaa                                                                                     >  2:2853666/1‑100 (MQ=255)
            ttCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTaaa                                                                                     <  1:565079/100‑1 (MQ=255)
             tacGGGTAACGGTGATAAAAATAGATGGCGACGTCTTTGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTGCCATTACACCATGAGGCTGTaaa                                                                                     <  1:41768/97‑1 (MQ=255)
                gggTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTg                                                                                 <  2:315000/100‑1 (MQ=255)
                   tAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAgg                                                                              <  1:2367236/100‑1 (MQ=255)
                   tAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAgg                                                                              <  1:2815817/100‑1 (MQ=255)
                    aaCGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGt                                                                             >  1:1545785/1‑100 (MQ=255)
                    aaCGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGt                                                                             <  2:2337804/100‑1 (MQ=255)
                     aCGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTc                                                                            >  1:1176754/1‑100 (MQ=255)
                      cGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCg                                                                           >  1:2368793/1‑100 (MQ=255)
                       ggTGATAAAAATCGATGGCGACATTTTTGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCga                                                                          <  1:2723239/100‑1 (MQ=255)
                       ggTGATAAAAATCGATGGCGACATCTTTGATATTCGCATTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCga                                                                          <  1:1959330/100‑1 (MQ=255)
                        gTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCgag                                                                         >  2:704882/1‑100 (MQ=255)
                         tGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCgag                                                                         >  1:945538/1‑99 (MQ=255)
                          gATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGAt                                                                       <  1:2472630/100‑1 (MQ=255)
                           aTAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATc                                                                      <  2:407716/100‑1 (MQ=255)
                            tAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATcc                                                                     >  2:2581612/1‑100 (MQ=255)
                            tAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATcc                                                                     >  2:1767414/1‑100 (MQ=255)
                             aaagaTCGATGGCGACATCTTTGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATccc                                                                    <  1:682365/100‑1 (MQ=255)
                                 aTCGATGGCGACATCTTTGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTg                                                                <  1:2668449/100‑1 (MQ=255)
                                 aTCGATGGCGACATCTTTGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTg                                                                <  2:2685531/100‑1 (MQ=255)
                                 aTCGATGGCGACATCTTTGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTg                                                                <  2:1667900/100‑1 (MQ=255)
                                 aTCGATGGCGACATCTTTGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTg                                                                >  1:2084745/1‑100 (MQ=255)
                                  tCGATGGCGACATCTTTGTTATTCTCTTTCCACAG‑GATGTGCGGTTCCATTTTTCCCATTACTCAATCAGGCTGTAAAGCTGTCGTCGAGAtgcgtgtgt                                                               <  1:226932/100‑9 (MQ=255)
                                  tCGATGGCGACATCTTTGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGc                                                               >  1:2544774/1‑100 (MQ=255)
                                  tCGATGGCGACATCTTTGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGc                                                               <  1:2138404/100‑1 (MQ=255)
                                   cGATGGCGACATCTTTGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGcc                                                              <  1:317672/100‑1 (MQ=255)
                                    gATGGCGACATCTTTGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCa                                                             <  1:2101290/100‑1 (MQ=255)
                                    gATGGCGACATCTTTGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCa                                                             >  2:2409058/1‑100 (MQ=255)
                                       ggCGACATCTTTGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATc                                                           >  1:1409217/1‑99 (MQ=255)
                                            cATCTTTGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTg                                                     <  1:2911512/100‑1 (MQ=255)
                                            cATCTTTGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTg                                                     <  1:448702/100‑1 (MQ=255)
                                              tCTTTGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTTCTg                                                     >  1:2569663/1‑98 (MQ=255)
                                              tCTTTGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGcc                                                   <  1:1451638/100‑1 (MQ=255)
                                               cTTTGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAGATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCa                                                  >  1:1039268/1‑100 (MQ=255)
                                               cTTTGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCa                                                  >  1:865791/1‑100 (MQ=255)
                                                tttGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAg                                                 >  2:1318020/1‑100 (MQ=255)
                                                tttGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAg                                                 <  1:2054901/100‑1 (MQ=255)
                                                tttGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAg                                                 >  1:2268456/1‑100 (MQ=255)
                                                 ttGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAgg                                                <  1:2440779/100‑1 (MQ=255)
                                                 ttGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAgg                                                <  1:1859661/100‑1 (MQ=255)
                                                 ttGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAgg                                                >  1:1246162/1‑100 (MQ=255)
                                                  tGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCAGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAgg                                                >  1:1868475/1‑99 (MQ=255)
                                                    atatTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCAc                                             <  2:372830/100‑1 (MQ=255)
                                                       ttCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCtt                                          <  2:2542175/100‑1 (MQ=255)
                                                        tctcTTTCCCCAG‑GATGCGCGGTTCCATTTTTTTTTTTTTTTCATGAAGCTGTAAATCTGAGGTCTTGTTCCCTTTTCCATCTTCCTGCCAGGCACCTTc                                         >  1:668617/1‑100 (MQ=255)
                                                        tctcTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTc                                         >  2:1545307/1‑100 (MQ=255)
                                                         ctctTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTcc                                        >  1:774935/1‑100 (MQ=255)
                                                         ctctTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTcc                                        >  2:2535162/1‑100 (MQ=255)
                                                         ctctTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTcc                                        <  1:576421/100‑1 (MQ=255)
                                                         ctctTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTcc                                        <  2:2222388/100‑1 (MQ=255)
                                                          tctTTCCCCAGCGAT‑CGCGGTTCTATCTTTCTCATTACGCCACGAGGCTGTATATTTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCa                                       >  1:2200332/1‑100 (MQ=255)
                                                          tctTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCa                                       >  1:2668294/1‑100 (MQ=255)
                                                            tttCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCAcc                                     <  2:774938/100‑1 (MQ=255)
                                                                cccAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGAt                                 <  2:1176759/100‑1 (MQ=255)
                                                                 ccAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGAtt                                <  1:1580034/100‑1 (MQ=255)
                                                                  cAG‑GATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTg                               >  2:409404/1‑100 (MQ=255)
                                                                    g‑gATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTgtc                             >  1:1768438/1‑98 (MQ=255)
                                                                    g‑gATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGc                             <  1:2776673/100‑1 (MQ=255)
                                                                    g‑gATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGc                             <  2:854836/100‑1 (MQ=255)
                                                                     cgacgcgcGGACCCATGTATCCCATTAAACCAACAGCCTGTAAATTTGCGCTCGAGCTTCATTTCCCGTATTAATGGAAGGCACCTTCCACCTGATTgg                              >  1:1178894/5‑99 (MQ=255)
                                                                        tGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGc                          >  2:1624665/1‑100 (MQ=255)
                                                                        tGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGc                          >  1:224417/1‑100 (MQ=255)
                                                                        tGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGc                          <  1:2409043/100‑1 (MQ=255)
                                                                         gcgcGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCt                         >  2:2140981/1‑100 (MQ=255)
                                                                         gcgcGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCt                         >  1:1444772/1‑100 (MQ=255)
                                                                          cgcgGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTg                        >  2:1888069/1‑100 (MQ=255)
                                                                           gcgGTTACATTTTTCCCATTACACCATGAGGATGTCAATCTGAGGTCGAGATCCCTTTGCCATATTCCTGCCAAGCACATTCCACCTGATTGGCAGCTg                        >  1:1129643/1‑99 (MQ=255)
                                                                            cgGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGtt                      >  1:1454335/1‑100 (MQ=255)
                                                                               ttCCATTTTTCCCATTACTCCATGTGGTTGTAAATCTGATGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGcgcaaca                       <  1:1993848/96‑7 (MQ=255)
                                                                               ttCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTgcg                   <  1:2473895/100‑1 (MQ=255)
                                                                               ttCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTgcg                   <  2:1894622/100‑1 (MQ=255)
                                                                                  cATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTgcgc                  <  2:1753756/98‑1 (MQ=255)
                                                                                  cATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCa                >  1:790109/1‑100 (MQ=255)
                                                                                  cATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCa                >  1:1709338/1‑100 (MQ=255)
                                                                                    tttttCCCATTACGCCATGAGGCTGTAAATCTGAGTTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCAcc              >  1:639615/1‑100 (MQ=255)
                                                                                    tttttCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCAcc              <  2:2492954/100‑1 (MQ=255)
                                                                                     ttttCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCAccc             <  1:364323/100‑1 (MQ=255)
                                                                                      tttCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCAcccc            <  1:1076705/100‑1 (MQ=255)
                                                                                      tttCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCAcccc            <  2:1094493/100‑1 (MQ=255)
                                                                                       ttCCCATTACACCATGAGGCTGTAAATCTGAGTTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCa           >  2:2764527/1‑100 (MQ=255)
                                                                                         cccATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCaaa         >  1:1474077/1‑100 (MQ=255)
                                                                                          ccATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAg        >  2:2400529/1‑100 (MQ=255)
                                                                                            aTTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGaa      >  2:1562240/1‑100 (MQ=255)
                                                                                              tACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAAt    >  2:243769/1‑100 (MQ=255)
                                                                                                cGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGt  >  2:1627186/1‑100 (MQ=255)
                                                                                                 |                                                                                                  
TAACGCGATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAG‑GATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGT  >  NC_000913/3903489‑3903683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A