Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F191 I1 R1 67 105.9 5535243 92.7% 5131170 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,903,585 G→A G39G (GGC→GGT)  bglB ← cryptic phospho‑beta‑glucosidase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,903,5850GA99.2% 435.1 / NA 124G39G (GGC→GGT) bglBcryptic phospho‑beta‑glucosidase B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (61/62);  minor base C (0/1);  total (61/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 124 total aligned reads displayed.

ATAACGCGATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAA  >  NC_000913/3903488‑3903680
                                                                                                 |                                                                                               
 tAACGCGATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCa                                                                                              <  2:561438/100‑1 (MQ=255)
 tAACGCGATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCa                                                                                              >  2:2575038/1‑100 (MQ=255)
 tAACGCGATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCa                                                                                              >  2:1608116/1‑100 (MQ=255)
 tAACGCGATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCa                                                                                              <  2:1897648/100‑1 (MQ=255)
   aCGCGATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATg                                                                                            <  2:1149850/100‑1 (MQ=255)
   aCGCGATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATg                                                                                            <  1:914332/100‑1 (MQ=255)
   aCGCGATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATg                                                                                            >  2:2712264/1‑100 (MQ=255)
   aCGCGATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATg                                                                                            <  1:420861/100‑1 (MQ=255)
    tgcgATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGa                                                                                           <  2:126312/99‑1 (MQ=255)
     gcgATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGaa                                                                                          >  1:126314/1‑99 (MQ=255)
          atCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGt                                                                                     <  2:828219/100‑1 (MQ=255)
           tCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTa                                                                                    <  2:1959161/100‑1 (MQ=255)
           tCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTa                                                                                    <  2:2261797/100‑1 (MQ=255)
             ttCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTaaa                                                                                  >  1:1154121/1‑100 (MQ=255)
             ttCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTaaa                                                                                  >  1:259479/1‑100 (MQ=255)
              tCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGTGGTTCTATTTTTCCCATTACACCATGAGGCTGTAAAt                                                                                 >  2:1856456/1‑100 (MQ=255)
               ccGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATc                                                                                <  2:1912902/100‑1 (MQ=255)
               ccGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATc                                                                                <  1:2669893/100‑1 (MQ=255)
                cGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCt                                                                               >  1:145793/1‑100 (MQ=255)
                 gggTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTg                                                                              <  2:2137506/100‑1 (MQ=255)
                 gggTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTg                                                                              <  2:708376/100‑1 (MQ=255)
                   gTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAg                                                                            >  1:579719/1‑100 (MQ=255)
                    tAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAgg                                                                           >  1:1397492/1‑100 (MQ=255)
                      aCGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTc                                                                         >  2:1751570/1‑100 (MQ=255)
                        ggTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCga                                                                       <  1:326788/100‑1 (MQ=255)
                         gTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCga                                                                       <  1:533310/99‑1 (MQ=255)
                           gATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGAt                                                                    >  1:210085/1‑100 (MQ=255)
                            aTAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGAt                                                                    >  2:2566001/1‑99 (MQ=255)
                            aTAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATc                                                                   >  2:1615656/1‑100 (MQ=255)
                              aaaaaTCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATccc                                                                 <  1:1608129/100‑1 (MQ=255)
                               aaaaTCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCt                                                                >  1:2570376/1‑100 (MQ=255)
                                aaaTCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCtt                                                               >  1:2574519/1‑100 (MQ=255)
                                  aTCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTg                                                             <  2:42729/100‑1 (MQ=255)
                                   tCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGc                                                            <  1:348488/100‑1 (MQ=255)
                                     gATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCa                                                          <  2:1487836/100‑1 (MQ=255)
                                      aTGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCAt                                                         <  1:956312/100‑1 (MQ=255)
                                        ggCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCt                                                       >  1:347074/1‑100 (MQ=255)
                                         gCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCtt                                                      <  2:2159769/100‑1 (MQ=255)
                                           gACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTcc                                                    <  1:1844752/100‑1 (MQ=255)
                                            aCATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCt                                                   <  1:425998/100‑1 (MQ=255)
                                             cATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTg                                                  <  1:1067787/100‑1 (MQ=255)
                                              aTCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGc                                                 <  2:1221869/100‑1 (MQ=255)
                                               tCTTTGATATTCTCTTTCCCCAGGATGCTCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGcc                                                <  1:507078/100‑1 (MQ=255)
                                               tCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGcc                                                >  1:391045/1‑100 (MQ=255)
                                               tCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGcc                                                <  2:176348/100‑1 (MQ=255)
                                                cTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCa                                               >  2:6666/1‑100 (MQ=255)
                                                cTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCa                                               >  1:2370560/1‑100 (MQ=255)
                                                cTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCa                                               >  2:2249293/1‑100 (MQ=255)
                                                 tttGATATTCTCTTTGCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAg                                              <  1:2720725/100‑1 (MQ=255)
                                                 tttGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAg                                              <  2:427130/100‑1 (MQ=255)
                                                 tttGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAg                                              <  1:2037046/100‑1 (MQ=255)
                                                 tttGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAg                                              <  2:528514/100‑1 (MQ=255)
                                                  ttGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGACGTCGAGATCCCTTTGCCTTCTTCCTGCCacg                                             <  1:1859657/100‑3 (MQ=255)
                                                   tGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACTATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGc                                            <  2:1041206/100‑1 (MQ=255)
                                                   tGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGc                                            <  1:1647638/100‑1 (MQ=255)
                                                   tGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGc                                            >  2:827304/1‑100 (MQ=255)
                                                    gATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCa                                           >  1:1041279/1‑100 (MQ=255)
                                                    cgatctctcTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCa                                           <  2:1551600/95‑1 (MQ=255)
                                                        ttCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCtt                                       >  1:1844135/1‑100 (MQ=255)
                                                        ttCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCtt                                       >  1:2478607/1‑100 (MQ=255)
                                                          ctctTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACCCCCTGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTcc                                     <  2:1938601/100‑1 (MQ=255)
                                                            ctTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCAc                                   <  1:1043667/100‑1 (MQ=255)
                                                            ctTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCAc                                   >  2:883847/1‑100 (MQ=255)
                                                            ctTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCAc                                   >  2:1773531/1‑100 (MQ=255)
                                                                ccccAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGa                               >  2:1565598/1‑100 (MQ=255)
                                                                ccccAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGa                               >  2:2008269/1‑100 (MQ=255)
                                                                 cccAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGAt                              <  1:766064/100‑1 (MQ=255)
                                                                 cccAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGAt                              >  2:850997/1‑100 (MQ=255)
                                                                 cccAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGAt                              >  2:1521706/1‑100 (MQ=255)
                                                                 cccAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGAt                              >  2:310632/1‑100 (MQ=255)
                                                                   tccgatctgcgGTTCCATTTTTCCCATTACACCATGAGGCTATAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGAttn                            <  1:1330387/92‑2 (MQ=255)
                                                                   cAGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTg                            >  1:1916318/1‑100 (MQ=255)
                                                                    aGGATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTgg                           >  2:852690/1‑100 (MQ=255)
                                                                     ggATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGc                          <  1:684665/100‑1 (MQ=255)
                                                                      gATGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCa                         <  2:2300797/100‑1 (MQ=255)
                                                                       aTGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGTGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGc                          <  2:644397/98‑1 (MQ=255)
                                                                       aTGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAg                        <  1:2712283/100‑1 (MQ=255)
                                                                        tGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGc                       <  2:1112419/100‑1 (MQ=255)
                                                                        tGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGc                       <  1:1565610/100‑1 (MQ=255)
                                                                        tGCGCGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGc                       <  1:2455043/100‑1 (MQ=255)
                                                                         gcgcGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCt                      >  2:977397/1‑100 (MQ=255)
                                                                         gcgcGGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCt                      >  1:2000504/1‑100 (MQ=255)
                                                                          cgcgGTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTg                     >  1:2002753/1‑100 (MQ=255)
                                                                          cgcgGTTCCA‑TTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGt                    >  1:1909060/1‑100 (MQ=255)
                                                                           ncgGTTCCATTTTTCCCATTACACCATGAGGCTATAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTgagatcgga                    >  2:1330378/2‑92 (MQ=255)
                                                                              gTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTgc                 >  2:2443359/1‑100 (MQ=255)
                                                                              gTTCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTgc                 <  2:815367/100‑1 (MQ=255)
                                                                                tCCATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTgcgc               >  2:797062/1‑100 (MQ=255)
                                                                                 ccATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGcc              >  1:3141/1‑100 (MQ=255)
                                                                                 ccATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGcc              <  2:3140/100‑1 (MQ=255)
                                                                                 ccATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGACAGCTGTTGCGcc              >  2:287580/1‑100 (MQ=255)
                                                                                  cATTTTTCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCa             >  1:2041080/1‑100 (MQ=255)
                                                                                     ttttCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCAccc          <  1:2124796/100‑1 (MQ=255)
                                                                                      tttCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCAcccc         <  2:2478588/100‑1 (MQ=255)
                                                                                      tttCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCAcccc         <  2:1844120/100‑1 (MQ=255)
                                                                                        tCCCATTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCaa       <  1:1522143/100‑1 (MQ=255)
                                                                                            aTTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGaa   >  1:1816427/1‑100 (MQ=255)
                                                                                            aTTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGaa   >  1:1704064/1‑100 (MQ=255)
                                                                                            aTTACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGaa   >  1:409782/1‑100 (MQ=255)
                                                                                             ttACACCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGaaa  <  2:685030/100‑1 (MQ=255)
                                                                                                 |                                                                                               
ATAACGCGATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAA  >  NC_000913/3903488‑3903680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A