Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F191 I0 R1	105	142.7	6924153	96.8%	6702580	99.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,551,608	G→A	46.5%	intergenic (‑312/‑28)	gntP ← / → uxuA	fructuronate transporter/mannonate hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,551,608	0	G	A	46.5%	30.7 / 191.8	142	intergenic (‑312/‑28)	gntP/uxuA	fructuronate transporter/mannonate hydrolase
Reads supporting (aligned to +/- strand): ref base G (36/40); new base A (39/27); total (75/67)
Fisher's exact test for biased strand distribution p-value = 1.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.41e-01

Only 100 of 147 total aligned reads displayed.

CTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGGAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCA  >  NC_000913/4551511‑4551707

cTTATACGTCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGGa                                                                                                    <  1:1009287/99‑1 (MQ=255)

  tataCGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGAAAcc                                                                                                 >  1:2914815/1‑100 (MQ=255)

     aCGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGGAACCCAc                                                                                              >  2:130507/1‑100 (MQ=255)

      cGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGAAACCCACt                                                                                             <  1:2690864/100‑1 (MQ=255)

       gCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGAAACCCACTa                                                                                            <  2:565728/100‑1 (MQ=255)

          aTCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGAAACCCACTAtgt                                                                                         >  2:2620934/1‑100 (MQ=255)

           tctcATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGGAACCCACTAtgtg                                                                                        <  2:379404/100‑1 (MQ=255)

           tctcATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGGAACCCACTAtgtg                                                                                        >  2:3358731/1‑100 (MQ=255)

             tcATCCGATGCAACGCCACGGCTGCGGTGTGGTTGTTCATCCGGATACCTAAACATCTCCAGGGTTCCGCGTCTCTTTGCTGTGAAACCCACTATGTGaa                                                                                      <  1:168285/100‑1 (MQ=255)

             tcATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGAAACCCACTATGTGaa                                                                                      <  1:717338/100‑1 (MQ=255)

              cATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGGAACCCACTATGTGaaa                                                                                     <  2:876849/100‑1 (MQ=255)

              cATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGGAACCCACTATGTGaaa                                                                                     >  2:2979549/1‑100 (MQ=255)

               aTCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGGAACCCACTATGTGAAag                                                                                    <  1:431146/100‑1 (MQ=255)

               aTCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGGAACCCACTATGTGAAag                                                                                    >  2:872676/1‑100 (MQ=255)

               aTCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGAAACCCACTATGTGAAag                                                                                    >  1:1464494/1‑100 (MQ=255)

               aTCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGAAACCCACTATGTGAAag                                                                                    >  2:442022/1‑100 (MQ=255)

                tCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGGAACCCACTATGTGAAaga                                                                                   >  2:1344426/1‑100 (MQ=255)

                 ccGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGGAACCCACTATGTGAAagag                                                                                  <  2:192223/100‑1 (MQ=255)

                  cGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGAAACCCACTATGTGAAAGAgg                                                                                 <  2:2438063/100‑1 (MQ=255)

                   gATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGAAACCCACTATGTGAAAGAGGa                                                                                >  1:2046052/1‑100 (MQ=255)

                   gATGCAACGCCACGGCTGCGGTCTGGTTGTTCAGCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGGAACCCACTATGTGAAAGAGGa                                                                                >  1:1022113/1‑100 (MQ=255)

                     tGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGAAACCCACTATGTGAAAGAGGaaa                                                                              >  2:421480/1‑100 (MQ=255)

                      gCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGATACCTAAACAACTCCAGGGTTCCGCGGCTCTTTGCTGTGGAACCCACTATGTGAAAGAGGaaaa                                                                             <  1:2869820/100‑1 (MQ=255)

                       cAACGCCGCGGCTGCGGTCTGGTGGTTCATCCGGATACCTAAACAGCTCCATTGTTCCGCGTCTCTTGGCTGAGGAACCTACTAGGTGAAAGAGGtaaaa                                                                            >  1:1370370/1‑100 (MQ=255)

                         aCGCCACGGCTGCGGTCTGGTTGTTCATCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGGAACCCACTATGTGAAAGAGGAAAAATc                                                                          >  1:3302708/1‑100 (MQ=255)

                          cGCCACGGCTGCGGTCTGGTTGTTCATCCGGATACCTCAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGAAACCCACTATGTGAAAGAGGAAAAATCa                                                                         >  1:1854202/1‑100 (MQ=255)

                            ccACGGCTGCGGTCTGGTTGTTCATCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGAAACCCACTATGTGAAAGAGGAAAAATCATg                                                                       >  1:335305/1‑100 (MQ=255)

                            ccACGGCTGCGGTCTGGTTGTTCATCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGAAACCCACTATGTGAAAGAGGAAAAATCATg                                                                       <  2:1772533/100‑1 (MQ=255)

                             cACGGCTGCGGTCTGGTTGTTCATCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGGAACCCACTATGTGAAAGAGGAAAAATCATg                                                                       >  2:157439/1‑99 (MQ=255)

                             cACGGCTGCGGTCTGGTTGTTCATCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGGAACCCACTATGTGAAAAAAAAAAAATCATgg                                                                      <  1:2602300/100‑1 (MQ=255)

                             cACGGCTGCGGTCTGGTTGTTCATCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGAAACCCACTATGTGAAAGAGGAAAAATCATgg                                                                      >  2:1857753/1‑100 (MQ=255)

                              aaggCTGCGGTCTGGTTGTTCATCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGGATCCCACTAAAAAAAAAAAAAAAAATCATgg                                                                      <  1:3043389/97‑1 (MQ=255)

                              aCGGCTGCGGTCTGGTTGTTCATCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGGAACCCACTATGTGAAAGAGGAAAAATCATGGa                                                                     <  2:2388467/100‑1 (MQ=255)

                              aCGGCTGCGGTCTGGTTGTTCATCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGGAACCCACTATGTGAAAGAGGAAAAATCATGGa                                                                     <  1:891083/100‑1 (MQ=255)

                              aCGGCTGCGGTCTGGTTGTTCATCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGGAACCCACTATGTGAAAGAGGAAAAATCATGGa                                                                     <  2:2676582/100‑1 (MQ=255)

                                ggCTGCGGTCTGGTTGTTCATCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGAAACCCACTATGTGAAAGAGGAAAAATCATGGAAc                                                                   >  2:719823/1‑100 (MQ=255)

                                     cGGTCTGGTTGTTCATCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGGAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGAcc                                                              <  1:3235591/100‑1 (MQ=255)

                                     cGGTCTGGTTGTTCATCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGGAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGAcc                                                              >  2:3239176/1‑100 (MQ=255)

                                      ggTCTGGTTGTTCATCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGGAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCt                                                             >  1:2530733/1‑100 (MQ=255)

                                       gTCTGGTTGTTCATCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGAAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTg                                                            >  1:2341899/1‑100 (MQ=255)

                                          aggTTGTTCATCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGAAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGgcg                                                         >  2:2562267/2‑100 (MQ=255)

                                           ggTTGTTCATCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGAAACCCCCTATGTGAAAGAGGAAAAATCATGGAACAGACCTGgcgc                                                        >  1:1666202/1‑100 (MQ=255)

                                           ggTTGTTCATCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGAAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGgcgc                                                        >  2:2833479/1‑100 (MQ=255)

                                            gttgttCATCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGGAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCt                                                       <  2:16614/100‑1 (MQ=255)

                                            gttgttCATCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGGAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCt                                                       <  1:2809358/100‑1 (MQ=255)

                                            gttgttCATCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGAAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCt                                                       >  1:1089662/1‑100 (MQ=255)

                                            gttgttCATCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGAAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCt                                                       <  1:50492/100‑1 (MQ=255)

                                             ttgttCATCCGGATACCTAAACAACTCCAGGGTTCCGCGTCACTTTGCTGTGAAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTg                                                      <  1:2224889/100‑1 (MQ=255)

                                               gttCATCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGGAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGt                                                    >  2:2159443/1‑100 (MQ=255)

                                                    tCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGGAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGc                                               <  2:2419914/100‑1 (MQ=255)

                                                    tCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGGAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGc                                               <  1:2670620/100‑1 (MQ=255)

                                                    tCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGGAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGc                                               <  2:590628/100‑1 (MQ=255)

                                                    tCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGAAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGc                                               <  2:1089671/100‑1 (MQ=255)

                                                     ccGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGGAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGcc                                              <  2:2962012/100‑1 (MQ=255)

                                                     ccGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGAAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGcc                                              <  1:3438120/100‑1 (MQ=255)

                                                      cGGATACCTAAACAACTCCAGGGTTCGGCGTCTCTTTGCTGTGAAACCCACTAAAAAAAAAAAAAAAAATCATGGAACAGACCTGGCGCTGGTACgg                                                <  1:727449/97‑1 (MQ=255)

                                                         aTACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGGAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGCCCaaa                                          >  2:1093944/1‑100 (MQ=255)

                                                          tACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGGAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGCCCAAAc                                         <  2:2177275/100‑1 (MQ=255)

                                                           aCCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGGAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGCCCAAACg                                        <  1:2979525/100‑1 (MQ=255)

                                                            ccTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGGAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGCCCAAACGa                                       >  2:965099/1‑100 (MQ=255)

                                                              tAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGGAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGCCCAAACGATc                                     <  2:1766201/100‑1 (MQ=255)

                                                              tAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGGAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGCCCAAACGATc                                     >  1:3090130/1‑100 (MQ=255)

                                                              tAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGGAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGCCCAAACGATc                                     >  1:3090131/1‑100 (MQ=255)

                                                                aacaacTCCAGGGTTCCGCGTCTCTTTGCTGTGGAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGCCCAAACGATCCg                                   <  1:130507/100‑1 (MQ=255)

                                                                aacaacTCCAGGGTGCCGCGTCTCTTTGCTGTGGAGCCCACTAGGGGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGCCCAAACGATc                                     <  1:3301100/98‑1 (MQ=255)

                                                                 acaacTCCAGGGTTCCGCGTCTCTTTGCTGTGGAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGCCCAAACGATCCgg                                  <  2:3206618/100‑1 (MQ=255)

                                                                  caacTCCAGGGTTCCGCGTCTCTTTGCTGTGGAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGCCCAAACGATCCGGt                                 >  2:1810886/1‑100 (MQ=255)

                                                                     cTCCAGGGTTCCGCGTCTCTTTGCTGTGGAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGCCCAAACGATCCGGTTTc                              <  1:1344414/100‑1 (MQ=255)

                                                                     cTCCAGGGTTCCGCGTCTCTTTGCTGTGGAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGCCCAAACGATCCGGTTTc                              >  1:1936420/1‑100 (MQ=255)

                                                                     cTCCAGGGTTCCGCGTCTCTTTGCTGTGAAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGCCCAAACGATCCGGTTTc                              >  1:2164450/1‑100 (MQ=255)

                                                                       ccAGGGTTCCGCGTCTCTTTGCTGTGGAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGCCCAAACGATCCGGTTTCtt                            >  1:525217/1‑100 (MQ=255)

                                                                       ccAGGGTTCCGCGTCTCTTTGCTGTGGAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGCCCAAACGATCCGGTTTCtt                            >  1:3404280/1‑100 (MQ=255)

                                                                       ccAGGGTTCCGCGTCTCTTTGCTGTGAAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGCCCAAACGATCCGGTTTCtt                            <  2:571093/100‑1 (MQ=255)

                                                                       ccAGGGTTCCGCGTCTCTTTGCTGTGAAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGCCCAAACGATCCGGTTTCtt                            >  1:522670/1‑100 (MQ=255)

                                                                       ccAGGGTTCCGCGTCTCTTTGCGGTGGAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGCCCAAACGATCCGGTTTCt                             <  1:1016836/99‑1 (MQ=255)

                                                                        cAGGGTTCCGCGTCTCTTTGCTGTGGAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGCCCAAACGATCCGGTTTCttt                           >  2:2832239/1‑100 (MQ=255)

                                                                        cAGGGTTCCGCGTCTCTTTGCTGTGGAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGCCCAAACGATCCGGTTTCttt                           >  2:3356721/1‑100 (MQ=255)

                                                                           ggTTCCGCGTCTCTTTGCTGTGGAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGCCCAAACGATCCGGTTTCTTTAGc                        <  1:3358707/100‑1 (MQ=255)

                                                                            gTTCCGCGTCTCTTTGCTGTGAAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGCCCAAACGATCCGGTTTCTTTAGCt                       >  1:1711132/1‑100 (MQ=255)

                                                                            gTTCCGCGTCTCTTTGCTGTGAAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGCCCAAACGATCCGGTTTCTTTAGCt                       <  2:2146605/100‑1 (MQ=255)

                                                                            gTTCCGCGTCTCTTTGCTGTGAAACCCACTATATGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGCCCAAACGATCCGGTTTCTTTAGc                        >  2:3281391/1‑99 (MQ=255)

                                                                             ttCCGCGTCTCTTTGCTGTGGAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGCCCAAACGATCCGGTTTCTTTAGCTg                      >  2:901344/1‑100 (MQ=255)

                                                                               ccGCGTCTCTTTGCTGTGGAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGCCCAAACGATCCGGTTTCTTTAGCTGAt                    <  2:1658836/100‑1 (MQ=255)

                                                                                  cgTCTCTTTGCTGTGGAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGCCCAAACGATCCGGTTTCTTTAGCTGATGTc                 <  2:1347306/100‑1 (MQ=255)

                                                                                  cgTCTCTTTGCTGTGAAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGCCCAAACGATCCGGTTTCTTTAGCTGATGTc                 <  1:2718158/100‑1 (MQ=255)

                                                                                   gTCTCTTTGCTGTGGAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGCCCAAACGATCCGGTTTCTTTAGCTGATGTcc                <  2:375205/100‑1 (MQ=255)

                                                                                   gTCTCTTTGCTGTGAAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGCCCAAACGATCCGGTTTCTTTAGCTGATGTcc                <  1:1373479/100‑1 (MQ=255)

                                                                                    tctcTTTGCTGTGAAACCCACTATGTGACAGAGGAAAAATAATGGAACAGACCTGGCGCTGGTACGGCCCAAACGATCCGGTTTCTTTAGCTGATGTCCg               >  2:1551411/1‑100 (MQ=255)

                                                                                     ctctTTGCTGTGAAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGt              >  1:2439688/1‑100 (MQ=255)

                                                                                      tctTTGCTGTGGAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTc             >  2:1268079/1‑100 (MQ=255)

                                                                                      tctTTGCTGTGGAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTc             >  1:1178801/1‑100 (MQ=255)

                                                                                        tttGCTTTAAAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTc             <  1:2849687/98‑1 (MQ=255)

                                                                                         ttGCTGTGAAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAgg          >  2:532318/1‑100 (MQ=255)

                                                                                            cTGTGGAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAggcgg       >  1:3189957/1‑100 (MQ=255)

                                                                                             tgtgAAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAggcggg      >  2:1747780/1‑100 (MQ=255)

                                                                                              gtgGAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAggcgggc     <  2:2352570/100‑1 (MQ=255)

                                                                                              gtgGAACCCACTATGTGAAAGAGGAAAAATCATGGAAAAGACCTGGCGCTGGTACGGCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAggcgggc     >  1:1855647/1‑100 (MQ=255)

                                                                                                ggAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGgcgc   >  2:745853/1‑100 (MQ=255)

                                                                                                ggAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGgcgc   <  2:2785103/100‑1 (MQ=255)

                                                                                                 gAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCa  <  1:2454434/100‑1 (MQ=255)

CTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGATACCTAAACAACTCCAGGGTTCCGCGTCTCTTTGCTGTGGAACCCACTATGTGAAAGAGGAAAAATCATGGAACAGACCTGGCGCTGGTACGGCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCA  >  NC_000913/4551511‑4551707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A