Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A16 F258 I0 R1 432 103.0 5191697 92.7% 4812703 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,165,032 G→A 22.7% H314H (CAC→CAT)  parC ← DNA topoisomerase IV, subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,165,0320GA22.7% 144.3 / 35.6 80H314H (CAC→CAT) parCDNA topoisomerase IV, subunit A
Reads supporting (aligned to +/- strand):  ref base G (37/24);  new base A (7/11);  total (45/35)
Fisher's exact test for biased strand distribution p-value = 1.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

GGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGATTCATCGCGCAGATCGTCAACC  >  NC_000913/3164935‑3165131
                                                                                                 |                                                                                                   
ggATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAgg                                                                                                     >  2:2050970/1‑98 (MQ=255)
 gATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAgatgg                                                                                                  >  2:1471382/1‑100 (MQ=255)
  aTTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAgatggt                                                                                                 <  2:1232901/100‑1 (MQ=255)
    ttCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGATGGTtc                                                                                               <  2:1183308/100‑1 (MQ=255)
     tCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTtca                                                                                              <  1:811327/100‑1 (MQ=255)
     tCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTtca                                                                                              >  2:2178858/1‑100 (MQ=255)
     tCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTtca                                                                                              <  1:2023093/100‑1 (MQ=255)
        agcagGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTtcatca                                                                                           <  2:611254/100‑1 (MQ=255)
          cagGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGCTATCTCTTTTCCAGATCGGTGGTAGCGAAGCGGTGGTTCATCAcc                                                                                         >  1:1815314/1‑100 (MQ=255)
           agGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCt                                                                                        <  2:1419481/100‑1 (MQ=255)
            ggTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTg                                                                                       >  2:1088449/1‑100 (MQ=255)
              tttttCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGAt                                                                                     <  1:822037/100‑1 (MQ=255)
              tttttCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGAGGTTCATAACCTGAt                                                                                     >  2:1135329/1‑100 (MQ=255)
               ttttCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATc                                                                                    <  1:866893/100‑1 (MQ=255)
                tttCACCGCCGGACGACCATCCCGCCCGATCATATTGAGGTGAATACGATCGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGGTCATCACCTGATcc                                                                                   <  1:2237050/100‑1 (MQ=255)
                tttCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATcc                                                                                   <  2:1992164/100‑1 (MQ=255)
                 ttCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCa                                                                                  >  1:2418120/1‑100 (MQ=255)
                  tCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGCGGTAGCGAAGAGGTGGTTCATCCCCTGATcc                                                                                   >  1:900299/1‑98 (MQ=255)
                   cACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCata                                                                                >  1:1717310/1‑100 (MQ=255)
                   cACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCata                                                                                >  2:2390176/1‑100 (MQ=255)
                   cACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCata                                                                                >  2:2518923/1‑100 (MQ=255)
                     ccgccgGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGAGGTAGCGAAAAGGTGGTTCATCACCTGATCCATATc                                                                              >  1:2119764/1‑100 (MQ=255)
                       gccgGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGATGGTTCATCACCTGATCCATATCCa                                                                            <  2:465157/100‑1 (MQ=255)
                       gccgGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGATGGTTCATCACCTGATCCATATCCa                                                                            <  2:464904/100‑1 (MQ=255)
                         cgGACGACCATCCAGACCGATCATATTAAGGTCAGTAGGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGCTGGTTCAGCACCTGAGCCATATCCAc                                                                           >  1:1266748/1‑99 (MQ=255)
                             cgacCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGt                                                                      >  1:814502/1‑100 (MQ=255)
                              gacCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGtt                                                                     <  2:971947/100‑1 (MQ=255)
                               acgagcctgACCGATCATAGTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCGCGCGGTTg                                                                    <  1:2426826/92‑1 (MQ=255)
                                ccATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTgg                                                                   >  1:2065769/1‑100 (MQ=255)
                                 cATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGa                                                                  >  2:582931/1‑100 (MQ=255)
                                 cATCCAGACCGATCATATTAAGGTAAATACGATAGCTGTTTTTCCGAGCGGTGGTAGCGAAGGGGTGGTGCATCACCTGATTCATATCCACGCGGTTg                                                                    <  1:33013/98‑1 (MQ=255)
                                  atgcAGACCGATCATATTAAGGTTAATACGATAGCTTTTTTCCAGATCGGTGGTAGCAAAGAGGTGTTTCATTATCTGATCCATATCGATGCGGTTGGaa                                                                 >  1:146514/4‑100 (MQ=255)
                                  aTCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGaa                                                                 >  2:2004316/1‑100 (MQ=255)
                                  aTCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGaa                                                                 <  1:52339/100‑1 (MQ=255)
                                      aGACCGATCATATTAAGGTTAATACGATAGCTCTTTCCGAGATCGGTGGTAGCGAAGAGGTGGTTGATCACCTGATCCATATCCACGCGGTTGGAAcgc                                                              >  1:2420271/1‑99 (MQ=255)
                                         ccGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGca                                                          >  1:2536105/1‑100 (MQ=255)
                                          cGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGcac                                                         <  2:982234/100‑1 (MQ=255)
                                          cGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGcac                                                         >  1:2355769/1‑100 (MQ=255)
                                           gATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGcaca                                                        >  2:1902384/1‑100 (MQ=255)
                                              cATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTGATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATc                                                     <  1:825662/100‑1 (MQ=255)
                                               atatTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCa                                                    >  1:423351/1‑100 (MQ=255)
                                                tatTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCAc                                                   >  1:2167565/1‑100 (MQ=255)
                                                tatTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGATGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCAc                                                   >  1:1205785/1‑100 (MQ=255)
                                                 atTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCAcc                                                  <  2:588442/100‑1 (MQ=255)
                                                 atTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCAcc                                                  >  1:509477/1‑100 (MQ=255)
                                                 atTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGATGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACGATCAcc                                                  <  2:387749/100‑1 (MQ=255)
                                                   tAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAg                                                >  1:1337110/1‑100 (MQ=255)
                                                      ggTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcg                                             >  1:2408807/1‑100 (MQ=255)
                                                       gTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcgg                                            >  2:77592/1‑100 (MQ=255)
                                                         tAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcgggt                                          >  1:946818/1‑100 (MQ=255)
                                                         tAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGATGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcgggt                                          >  1:552854/1‑100 (MQ=255)
                                                           aTACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGATGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcgggtcg                                        >  1:2081536/1‑100 (MQ=255)
                                                             aCGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcgggtcggg                                      <  2:657378/100‑1 (MQ=255)
                                                             aCGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcgggtcggg                                      >  1:1024655/1‑100 (MQ=255)
                                                                   gCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCg                                <  1:2020643/100‑1 (MQ=255)
                                                                    ctctTTTCCTGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGt                               >  2:1344810/1‑100 (MQ=255)
                                                                    ctctTTTCCCGATCGGTGGTAGCGAAGAGATGGTTCATCACCTGATCCATATCGACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGt                               >  1:127007/1‑100 (MQ=255)
                                                                     tctTTTCCAGATCGGTGGTAGCGAAGAGATGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTg                              <  2:101375/100‑1 (MQ=255)
                                                                     tctTTTCCAGATCAGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTg                              >  1:1600872/1‑100 (MQ=255)
                                                                        tttCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTc                           >  2:1139078/1‑100 (MQ=255)
                                                                          tCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAg                         <  1:2039817/100‑1 (MQ=255)
                                                                          tCCAGATCGGTGGTAGCGAAGAGATGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAg                         >  2:1697474/1‑100 (MQ=255)
                                                                           ccAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGa                        >  2:2483073/1‑100 (MQ=255)
                                                                           ccAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGa                        >  2:1497493/1‑100 (MQ=255)
                                                                             aGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGAtt                      >  1:814406/1‑100 (MQ=255)
                                                                                tCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGATTCAt                   >  1:1679817/1‑100 (MQ=255)
                                                                                  ggtggtAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGATTCATcg                 <  2:2076447/100‑1 (MQ=255)
                                                                                  ggtggtAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGATTCATcg                 >  2:1223798/1‑100 (MQ=255)
                                                                                  ggtggtAGCGAAGAGATGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGATTCATcg                 >  2:17317/1‑100 (MQ=255)
                                                                                   gtggtAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGATTCATcgc                <  1:1444060/100‑1 (MQ=255)
                                                                                   gtggtAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGATTCATcgc                <  2:2406937/100‑1 (MQ=255)
                                                                                    tggtAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGATTCATcgcg               <  2:2414971/100‑1 (MQ=255)
                                                                                    tggtAGCGAAGAGATGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGATTCATcgcg               <  2:812085/100‑1 (MQ=255)
                                                                                     ggtAGCGAAGAGATGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGATTCATcgcg               <  1:552587/99‑1 (MQ=255)
                                                                                      tctgCGACGAGATGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGATTCATCGCGCa             <  1:1385810/97‑1 (MQ=255)
                                                                                      gtAGCGAAGAGATGGTTCATCACCTGATCCATATCCAAGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGATTCATCgcgc              <  1:365343/99‑1 (MQ=255)
                                                                                       tAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGATTCATCGCGCAg            <  1:433265/100‑1 (MQ=255)
                                                                                         gCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCCCCAGGCGCGTCGGGTTCTCGTGGTCAGATTCATCGCGCCGAt          >  1:865766/1‑100 (MQ=255)
                                                                                         gCGAAGAGATGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGATTCATCGCGCaaga          >  2:1385818/1‑97 (MQ=255)
                                                                                          cGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGATTCATCGCGCAGATc         >  2:492750/1‑100 (MQ=255)
                                                                                             agagGTGGTTCATCGCCTGATGCTTATCGACGCGGTTGGAACGCGGCACAATCGCCAGGCGGGTCGGGTTCTCGTGGTCAGATTCATCGCGCAGATc         <  1:2175694/97‑1 (MQ=255)
                                                                                             agagATGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGATTCATCGCGCAGATCGTc      <  2:540974/100‑1 (MQ=255)
                                                                                             agagATGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGATTCATCGCGCAGATCGTc      <  2:828897/100‑1 (MQ=255)
                                                                                                ggtggtTCATCAGCTGATCCATATCCACGCGGCTCGAACGCGGCACACTCACCAGGCGGGTCGGGTTCTCGTGGTCAGAATCATCGCGCAGATCGTCacc   >  1:1637827/1‑98 (MQ=255)
                                                                                                ggtggtTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGATTCATCGCGCAGATCGTCaa    >  1:2448167/1‑99 (MQ=255)
                                                                                                ggtggtTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGATTCATCGCGCAGATCGTCAAc   <  1:372483/100‑1 (MQ=255)
                                                                                                 gtggtTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGAGTCATCGCGCAGATCGTCAAcc  >  1:1535965/1‑100 (MQ=255)
                                                                                                 |                                                                                                   
GGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGATTCATCGCGCAGATCGTCAACC  >  NC_000913/3164935‑3165131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A