Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A9 F200 I1 R1
|
100 |
75.0 |
3643689 |
96.7% |
3523447 |
99.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,952,380 |
G→A |
L60L (CTG→CTA) |
ilvM → |
acetolactate synthase 2 small subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,952,380 | 0 | G | A | 97.5%
| 267.8
/ ‑2.2
| 81 | L60L (CTG→CTA) | ilvM | acetolactate synthase 2 small subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/2); new base A (40/39); total (40/41) |
| Fisher's exact test for biased strand distribution p-value = 4.94e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.98e-01 |
TTTCCACGTCTGCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATCACAACAAATCCGCGCCTGAGCGCAAAAGGAA > NC_000913/3952284‑3952476
|
tttCCACGTCTGCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTAttt < 2:228045/100‑1 (MQ=255)
tttCCACGTCTGCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTAttt < 1:392082/100‑1 (MQ=255)
tCCACGTCTGCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAg < 2:1086710/100‑1 (MQ=255)
tCCACGTCTGCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAg > 1:422359/1‑100 (MQ=255)
tCCACGTCTGCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAg < 2:343635/100‑1 (MQ=255)
cACGTCTGCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTc < 2:922434/100‑1 (MQ=255)
cGTCTGCTCACTGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGGCCACGGTCGGTCGACTTACTATTTAGTCAg < 1:1191045/100‑1 (MQ=255)
gTCTGCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAg > 1:1533634/1‑99 (MQ=255)
tCTGCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGtt < 1:1431824/100‑1 (MQ=255)
cTGCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTta < 1:78417/100‑1 (MQ=255)
cTGCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTta > 2:1005819/1‑100 (MQ=255)
tGCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTta < 2:225220/99‑1 (MQ=255)
gCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTtaaa > 2:1264070/1‑100 (MQ=255)
gCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTtaaa < 2:19887/100‑1 (MQ=255)
gCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTtaaa > 2:1709728/1‑100 (MQ=255)
cTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTtaaat < 1:1360215/100‑1 (MQ=255)
cAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTtaaataa > 1:36437/1‑100 (MQ=255)
aaTGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTtaaataaa > 2:1115511/1‑100 (MQ=255)
aTGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAAc < 1:1305696/100‑1 (MQ=255)
atatGGCCGCCGCCTGCGATGCATAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACtgg < 2:132196/99‑1 (MQ=255)
atatGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACtggt < 1:1042738/100‑1 (MQ=255)
tatGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACtggtg < 2:32280/100‑1 (MQ=255)
atGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACtggtgg < 1:26403/100‑1 (MQ=255)
atGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACtggtgg < 1:1014176/100‑1 (MQ=255)
atGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACtggtg > 1:772580/1‑99 (MQ=255)
gccgccgccAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACg > 1:1654651/1‑100 (MQ=255)
gccgccgccAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACg > 1:288429/1‑100 (MQ=255)
ccgccgccAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGt < 1:1412863/100‑1 (MQ=255)
gccgccAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCg < 2:1675658/100‑1 (MQ=255)
cgccAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGca < 1:1335643/100‑1 (MQ=255)
cgccAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGca > 2:778434/1‑100 (MQ=255)
gccAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGcac < 2:1017268/100‑1 (MQ=255)
aGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACg > 2:1690074/1‑100 (MQ=255)
aTGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGc > 1:1134914/1‑100 (MQ=255)
aTGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGc > 2:17319/1‑100 (MQ=255)
tGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGcc < 1:205780/100‑1 (MQ=255)
tGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGcc < 2:422361/100‑1 (MQ=255)
tGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGAACACGTTGcc > 1:39267/1‑100 (MQ=255)
gCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCa < 1:617025/100‑1 (MQ=255)
gCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCa < 2:1677837/100‑1 (MQ=255)
cacaAAATATAAATATCGAATTGACCGTTGACAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCAGACGTTGCCAt > 1:1192262/1‑100 (MQ=255)
acaAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATc > 2:1671287/1‑100 (MQ=255)
acaAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATc > 1:1262637/1‑100 (MQ=255)
aaaaTATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTg < 2:1630079/100‑1 (MQ=255)
aaaaTATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTg < 1:996996/100‑1 (MQ=255)
tataaatatCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCag < 2:790453/100‑1 (MQ=255)
atatCGAATTGACCGTTGCCAGCCAACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGcac > 1:1264810/1‑100 (MQ=255)
atCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACaa > 2:922403/1‑100 (MQ=255)
tCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAAc < 1:221437/100‑1 (MQ=255)
tCGAATTGACCGTTGCCACCCCACGGTCGGTCGACTTACTATTGAGTCAGTTACATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAAc < 1:1162525/100‑1 (MQ=255)
cGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAAcc < 1:773123/100‑1 (MQ=255)
cGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAAcc > 2:1126399/1‑100 (MQ=255)
gAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACca < 1:778429/100‑1 (MQ=255)
gAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACca > 2:1814746/1‑100 (MQ=255)
aTTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACcaca > 2:1301580/1‑100 (MQ=255)
gACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATca > 2:991554/1‑100 (MQ=255)
gACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATca < 2:1262648/100‑1 (MQ=255)
aCCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATcac > 1:1468243/1‑100 (MQ=255)
ccGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATcac > 1:134309/1‑99 (MQ=255)
gTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATCacaac < 2:288431/100‑1 (MQ=255)
ttGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATCacaac < 1:1054118/99‑1 (MQ=255)
tGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATCAcaacaa > 2:1454080/1‑100 (MQ=255)
tGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATCAcaacaa > 2:1767695/1‑100 (MQ=255)
cAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATCACAACAAAt > 1:366126/1‑99 (MQ=255)
ccccgatcggtcgACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGCGCACAACCACATCACAAGAAATCcg < 1:613814/92‑1 (MQ=255)
ccACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATCACAACAAATCcgcg > 2:1484397/1‑100 (MQ=255)
cACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGAGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATCACAACAAATCCgcgc < 1:1277297/100‑1 (MQ=255)
aCGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATCACAACAAATCCGCGcc > 1:1747479/1‑100 (MQ=255)
cggtcggtCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGGTGCCATCTGCCATAGCACAACCACATCACAACAAATCCGCGCCt > 2:989733/1‑100 (MQ=255)
ggtcggtcGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATCACAACAAATCCGCGCCTg < 1:922397/100‑1 (MQ=255)
gtcggtcgACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATCACAACAAATCCGCGCCTg < 1:567762/99‑1 (MQ=255)
cggtcgACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATCACAACAAATCCGCGCCTGAgc > 2:1708375/1‑100 (MQ=255)
ggtcgACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATCACAACAAATCCGCGCCTGAgcg > 2:643625/1‑100 (MQ=255)
gACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATCACAACAAATCCGCGCCTGAGCGCaaa > 1:145142/1‑100 (MQ=255)
cTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATCACAACAAATCCGCGCCTGAGCGCAAAAg > 2:1594852/1‑100 (MQ=255)
cTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATCACAACAAATCCGCGCCTGAGCGCAAAAg > 2:19392/1‑100 (MQ=255)
cTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATCACAACAAATCCGCGCCTGAGCGCAAAAg > 2:1508289/1‑100 (MQ=255)
cTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATCACAACAAATCCGCGCCTGAGCGCAAAAg < 2:1533645/100‑1 (MQ=255)
ttACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATCACAACAAATCCGCGCCTGAGCGCAAAAgg < 2:1042382/100‑1 (MQ=255)
tACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATCACAACAAATCCGCGCCTGAGCGCAAAAGGa > 1:1088293/1‑100 (MQ=255)
aCTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATCACAACAAATCCGCGCCTGAGCGCAAAAGGaa > 1:541768/1‑100 (MQ=255)
|
TTTCCACGTCTGCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATCACAACAAATCCGCGCCTGAGCGCAAAAGGAA > NC_000913/3952284‑3952476
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
