Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F244 I1 R1 118 120.6 6073099 93.5% 5678347 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,468,389 T→C S489G (AGT→GGT)  chiA ← periplasmic endochitinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,468,3890TC97.1% 353.9 / ‑0.6 104S489G (AGT→GGT) chiAperiplasmic endochitinase
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base C (48/53);  total (49/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

Only 100 of 105 total aligned reads displayed.

TCTTCGTTGCTATAGCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGTTC  >  NC_000913/3468292‑3468485
                                                                                                 |                                                                                                
tctTCGTTGCTATAGCTCTGATCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACctt                                                                                                >  1:2725960/1‑100 (MQ=255)
 ctTCGTTGCTATAGCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTg                                                                                               <  1:132008/100‑1 (MQ=255)
 ctTCGTTGCTATAGCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTg                                                                                               >  2:1650641/1‑100 (MQ=255)
 ctTCGTTGCTATAGCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTg                                                                                               >  1:2563234/1‑100 (MQ=255)
     gTTGCTATAGCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTc                                                                                           >  1:818667/1‑100 (MQ=255)
       tGCTATAGCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCa                                                                                         <  1:997548/100‑1 (MQ=255)
       tGCTATAGCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCa                                                                                         <  2:2916379/100‑1 (MQ=255)
        gCTATAGCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAg                                                                                        >  1:66974/1‑100 (MQ=255)
         cTATAGCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGa                                                                                       <  2:1468699/100‑1 (MQ=255)
         cTATAGCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGa                                                                                       >  1:656557/1‑100 (MQ=255)
         cTATAGCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGa                                                                                       <  1:2750803/100‑1 (MQ=255)
         cTATAGCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGa                                                                                       >  2:1965065/1‑100 (MQ=255)
          nataGCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGaa                                                                                      >  2:415401/2‑100 (MQ=255)
           ataGCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAAc                                                                                     >  1:1176376/1‑100 (MQ=255)
            taGCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACa                                                                                    >  2:1018497/1‑100 (MQ=255)
              gCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAgc                                                                                  <  1:422483/100‑1 (MQ=255)
              gCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAgc                                                                                  >  2:32717/1‑100 (MQ=255)
               ctctGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAgcg                                                                                 <  1:1346102/100‑1 (MQ=255)
               ctctGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGAGTTGTCACCTTGCGTCCAGAACAgcg                                                                                 <  1:1846107/100‑1 (MQ=255)
                tctGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAgcgc                                                                                <  1:203117/100‑1 (MQ=255)
                 ctGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCt                                                                               <  1:1851479/100‑1 (MQ=255)
                 ctGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCt                                                                               >  2:71013/1‑100 (MQ=255)
                 ctGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGAAGGGTTGTCACCTTGCGTCCAGAANAGCGCt                                                                               >  1:2319633/1‑100 (MQ=255)
                  tGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCtt                                                                              <  2:1691070/100‑1 (MQ=255)
                   gAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTc                                                                             <  2:2974037/100‑1 (MQ=255)
                   gAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTc                                                                             <  2:150948/100‑1 (MQ=255)
                      ccTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATa                                                                          <  1:1929780/100‑1 (MQ=255)
                      ccTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATa                                                                          <  1:1302042/100‑1 (MQ=255)
                        ttACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAg                                                                        <  2:1375789/100‑1 (MQ=255)
                         tACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGc                                                                       >  2:2014747/1‑100 (MQ=255)
                          aCCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAg                                                                        <  1:1739818/98‑1 (MQ=255)
                           ccTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCt                                                                     >  2:1301371/1‑100 (MQ=255)
                           ccTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCt                                                                     >  2:1723006/1‑100 (MQ=255)
                            cTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGTAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGccgg                                                                    <  1:2048523/100‑3 (MQ=255)
                             tAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGc                                                                   >  1:1263813/1‑100 (MQ=255)
                              aaCGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGcc                                                                  >  1:2354335/1‑100 (MQ=255)
                              aaCGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGcc                                                                  >  1:1544518/1‑100 (MQ=255)
                                cGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCa                                                                >  2:15869/1‑100 (MQ=255)
                                cGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCa                                                                <  1:870741/100‑1 (MQ=255)
                                  gCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATc                                                              >  1:1365298/1‑100 (MQ=255)
                                   cTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATca                                                             <  1:1650655/100‑1 (MQ=255)
                                   cTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATca                                                             <  2:1805764/100‑1 (MQ=255)
                                   cTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATca                                                             >  1:1362188/1‑100 (MQ=255)
                                     tCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATcaac                                                           <  1:2218358/100‑1 (MQ=255)
                                     tCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATcaa                                                            <  2:2725936/99‑1 (MQ=255)
                                      ccACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATcaaca                                                          <  1:468990/100‑1 (MQ=255)
                                       cACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATcaacaa                                                         >  2:2798560/1‑100 (MQ=255)
                                        aCGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATcaacaan                                                        <  1:415405/100‑2 (MQ=255)
                                        aCGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATcaacaac                                                        <  2:2874654/100‑1 (MQ=255)
                                        aCGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATcaacaac                                                        >  2:2835917/1‑100 (MQ=255)
                                            gcggcTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAAcg                                                    <  1:1018507/100‑1 (MQ=255)
                                               gcTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGa                                                 <  1:2411552/100‑1 (MQ=255)
                                                 tATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATc                                               <  2:1951435/100‑1 (MQ=255)
                                                  aTTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCa                                              >  2:585669/1‑100 (MQ=255)
                                                    tGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATGAGCCTGCCCATCAACAACAACGCGATCAcc                                            >  2:1770534/1‑100 (MQ=255)
                                                    tGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCAcc                                            <  2:1596971/100‑1 (MQ=255)
                                                    tGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCAcc                                            <  1:2327575/100‑1 (MQ=255)
                                                     gatACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCt                                           >  1:2811978/3‑100 (MQ=255)
                                                        aCCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTc                                        <  1:2798585/100‑1 (MQ=255)
                                                         ccGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCa                                       <  1:2355896/100‑1 (MQ=255)
                                                         ccGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCa                                       <  2:818071/100‑1 (MQ=255)
                                                            gTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGt                                    >  2:1636373/1‑100 (MQ=255)
                                                             tGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTa                                   <  1:261761/100‑1 (MQ=255)
                                                             tGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACATTTCACGTa                                   >  2:2901012/1‑100 (MQ=255)
                                                              ggCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTac                                  <  1:1732075/100‑1 (MQ=255)
                                                                cGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTacac                                >  2:2522056/1‑100 (MQ=255)
                                                                cGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTacac                                <  1:434508/100‑1 (MQ=255)
                                                                  ttttGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAg                              <  1:2656824/100‑1 (MQ=255)
                                                                    ttGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTg                            >  1:1589253/1‑100 (MQ=255)
                                                                     tGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTgg                           <  2:1949569/100‑1 (MQ=255)
                                                                     tGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTgg                           >  1:1568315/1‑100 (MQ=255)
                                                                     tGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTgg                           <  2:2306107/100‑1 (MQ=255)
                                                                     tGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTgg                           <  2:1365285/100‑1 (MQ=255)
                                                                      aaaacGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGa                          >  1:694898/5‑100 (MQ=255)
                                                                       gTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGAt                         >  1:320862/1‑100 (MQ=255)
                                                                       gTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGAt                         >  1:2532657/1‑100 (MQ=255)
                                                                        ttCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATg                        >  1:952317/1‑100 (MQ=255)
                                                                        aacGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATg                        <  1:1591638/98‑1 (MQ=255)
                                                                          cGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCg                      >  2:2607195/1‑100 (MQ=255)
                                                                           gCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCgg                     >  1:826021/1‑100 (MQ=255)
                                                                           gCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCgg                     >  2:3010105/1‑100 (MQ=255)
                                                                            ccaccaGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGa                    >  1:2727548/1‑100 (MQ=255)
                                                                            ccaccaGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGa                    >  2:3030496/1‑100 (MQ=255)
                                                                             caccaGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGAc                   >  1:2673185/1‑100 (MQ=255)
                                                                             caccaGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGAc                   >  1:1364881/1‑100 (MQ=255)
                                                                             caccaGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGAc                   <  1:71014/100‑1 (MQ=255)
                                                                              accaGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGAcc                  >  1:810292/1‑100 (MQ=255)
                                                                              accaGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGAcc                  <  2:1437157/100‑1 (MQ=255)
                                                                              accaGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGAcc                  >  1:2164247/1‑100 (MQ=255)
                                                                               ccaGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCa                 >  2:112148/1‑100 (MQ=255)
                                                                                    gCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCtt            >  1:970730/1‑100 (MQ=255)
                                                                                     cAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCttt           <  2:2884998/100‑1 (MQ=255)
                                                                                       gggTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCg         <  2:656550/100‑1 (MQ=255)
                                                                                       gggTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCg         <  2:814724/100‑1 (MQ=255)
                                                                                        ggTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCgn        <  2:952308/100‑2 (MQ=255)
                                                                                          ttGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCg      <  1:1654709/100‑1 (MQ=255)
                                                                                           tGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCgg     <  2:1001816/100‑1 (MQ=255)
                                                                                           tGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCgg     >  1:618821/1‑100 (MQ=255)
                                                                                            gTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGt    >  2:85424/1‑100 (MQ=255)
                                                                                              cACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGCCCATGCTTTCGCCGGTTc  >  1:2816567/1‑100 (MQ=255)
                                                                                                 |                                                                                                
TCTTCGTTGCTATAGCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGTTC  >  NC_000913/3468292‑3468485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A