Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F244 I1 R1 118 120.6 6073099 93.5% 5678347 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,657,494 T→C L167P (CTG→CCG)  hdeD → acid‑resistance membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,657,4940TC97.7% 446.2 / ‑1.4 132L167P (CTG→CCG) hdeDacid‑resistance membrane protein
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base C (61/67);  total (63/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01

Only 100 of 134 total aligned reads displayed.

AGCTGGCTACAGCTCGTTATTGGTGTGCTGGATATCGTCATTGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATC  >  NC_000913/3657397‑3657590
                                                                                                 |                                                                                                
aGCTGGCTACAGCTCGTTATTGGTGTGCTGGATATCGTCATTGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACcgg                                                                                                >  1:2598862/1‑100 (MQ=255)
   tGGCTACAGCTCGTTATTGGTGTGCTGGATATCGTCATTGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACcggtgg                                                                                             >  2:2914908/1‑100 (MQ=255)
    ggCTACAGCTCGTTATTGGTGTGCTGGATATCGTCATTGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTggg                                                                                            <  1:2170240/100‑1 (MQ=255)
    ggCTACAGCTCGTTATTGGTGTGCTGGATATCGTCATTGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTggg                                                                                            >  2:2391153/1‑100 (MQ=255)
     gCTACAGCTCGTTATTGGTGTGCTGGATATCGTCATTGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGa                                                                                           >  1:366937/1‑100 (MQ=255)
     gCTACAGCTCGTTATTGGTGTGCTGGATATCGTCATTGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGa                                                                                           >  1:144481/1‑100 (MQ=255)
      cTACAGCTCGTTATTGGTGTGCTGGATATCGTCATTGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGaa                                                                                          <  1:901313/100‑1 (MQ=255)
      cTACAGCTCGTTATTGGTGTGCTGGATATCGTCATTGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGaa                                                                                          >  2:1507227/1‑100 (MQ=255)
       tACAGCTCGTTATTGGTGTGCTGGATATCGTCATTGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAAt                                                                                         >  1:2363174/1‑100 (MQ=255)
           gCTCGTTATTGGTGTGCTGGATATCGTCATTGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGaa                                                                                     >  2:2596820/1‑100 (MQ=255)
           gCTCGTTATTGGTGTGCTGGATATCGTCATTGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGaa                                                                                     >  1:977474/1‑100 (MQ=255)
           gCTCGTTATTGGTGTGCTGGATATCGTCATTGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGaa                                                                                     >  2:1816975/1‑100 (MQ=255)
           gCTCGTTATTGGTGTGCTGGATATCGTCATTGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGATGGTTTCCACACCGGTGGGAATTg                                                                                       <  2:1000188/98‑1 (MQ=255)
            cTCGTTATTGGTGTGCTGGATATCGTCATTGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAAc                                                                                    >  1:1546198/1‑100 (MQ=255)
            cTCGTTATTGGTGTGCTGGATATCGTCATTGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAAc                                                                                    >  1:565719/1‑100 (MQ=255)
            cTCGTTATTGGTGTGCTGGATATCGTCATTGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAAc                                                                                    >  1:331991/1‑100 (MQ=255)
            cTCGTTATTGGTGTGCTGGATATCGTCATTGCCTGGACATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAAc                                                                                    <  2:2038354/100‑1 (MQ=255)
             tCGTTATTGGTGTGCTGGATATCGTCATTGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACt                                                                                   <  1:1871458/100‑1 (MQ=255)
              cGTTATTGGTGTGCTGGATATCGTCATTGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTg                                                                                  <  1:2295951/100‑1 (MQ=255)
                ttATTGGTGTGCTGGATATCGTCATTGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGat                                                                                <  2:1808372/100‑1 (MQ=255)
                   ttGGTGTGCTGGATATCGTCATTGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATAtt                                                                             >  2:2421330/1‑100 (MQ=255)
                     ggTGTGCTGGATATCGTCATTGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTa                                                                           >  2:684166/1‑100 (MQ=255)
                     ggTGTGCTGGATATCGTCATTGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTa                                                                           >  1:1974390/1‑100 (MQ=255)
                     ggTGTGCTGGATATCGTCATTGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTa                                                                           >  1:2972035/1‑100 (MQ=255)
                        gtgCTGGATATCGTCATTGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAgcg                                                                        >  2:2805163/1‑100 (MQ=255)
                        gtgCTGGATATCGTCATTGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAgcg                                                                        >  1:2081454/1‑100 (MQ=255)
                         tgCTGGATATCGTCATTGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAgcgc                                                                       <  2:1723167/100‑1 (MQ=255)
                          gCTGGATATCGTCATTGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGcgcc                                                                      >  2:2332983/1‑100 (MQ=255)
                            tGGATATCGTCATTGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGcgccgc                                                                    >  2:2381586/1‑100 (MQ=255)
                            tGGATATCGTCATTGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGcgccgc                                                                    >  2:416923/1‑100 (MQ=255)
                            tGGATATCGTCATTGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGcgccg                                                                     >  1:1197604/1‑99 (MQ=255)
                                tatCGTCATTGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGc                                                                <  1:228417/100‑1 (MQ=255)
                                 atCGTCATTGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGcc                                                               <  2:128690/100‑1 (MQ=255)
                                 atCGTCATTGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGcc                                                               <  2:1364373/100‑1 (MQ=255)
                                  tCGTCATTGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCt                                                              <  2:540624/100‑1 (MQ=255)
                                   cGTCATTGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTg                                                             >  1:2648302/1‑100 (MQ=255)
                                      cATTGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTc                                                          <  1:24982/100‑1 (MQ=255)
                                      cATTGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTc                                                          >  1:2739066/1‑100 (MQ=255)
                                       gttGGCTGGATATTCCTTGGTGCACCGTGGATGGTGTCTGTAACGCTGGTGGCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCa                                                         <  2:2099452/99‑1 (MQ=255)
                                       aTTGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCa                                                         <  1:181212/100‑1 (MQ=255)
                                       aTTGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCa                                                         <  2:105391/100‑1 (MQ=255)
                                         tGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTACCGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTGAGCGCCGCCAGCCTGTTCa                                                         <  2:2773334/98‑1 (MQ=255)
                                         tGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAgn                                                       <  2:1376057/100‑2 (MQ=255)
                                         tGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGc                                                       <  1:1250622/100‑1 (MQ=255)
                                           ccTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCtt                                                     >  2:669908/1‑100 (MQ=255)
                                             tGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCg                                                   >  1:3008408/1‑100 (MQ=255)
                                             tGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCg                                                   <  1:3006554/100‑1 (MQ=255)
                                             tGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCg                                                   <  1:2842874/100‑1 (MQ=255)
                                              ggATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGc                                                  <  2:927883/100‑1 (MQ=255)
                                               gATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGcc                                                 <  1:2381608/100‑1 (MQ=255)
                                                 atctCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAg                                               <  1:81695/97‑1 (MQ=255)
                                                   ttCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGtt                                             <  2:1152393/100‑1 (MQ=255)
                                                   ttCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGtt                                             >  1:1568984/1‑100 (MQ=255)
                                                    tCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGTAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGttt                                            <  2:745134/100‑1 (MQ=255)
                                                     ccTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAgagat                                           >  2:81694/1‑96 (MQ=255)
                                                     ccTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTg                                           >  1:644321/1‑100 (MQ=255)
                                                     ccTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTg                                           >  2:237906/1‑100 (MQ=255)
                                                      cTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACGCCGGTGGGAATTGAACTGAGATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGt                                          >  2:1657867/1‑100 (MQ=255)
                                                      cTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGt                                          >  1:1016763/1‑100 (MQ=255)
                                                      cTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGt                                          >  2:2891167/1‑100 (MQ=255)
                                                      cTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGt                                          <  2:2630067/100‑1 (MQ=255)
                                                      cTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGt                                          >  1:2948738/1‑100 (MQ=255)
                                                      cTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGt                                          <  1:2914757/100‑1 (MQ=255)
                                                         ggTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCg                                       <  1:1720251/100‑1 (MQ=255)
                                                          gTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACCCCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCg                                       <  2:1659666/99‑1 (MQ=255)
                                                          gTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGt                                      <  1:238698/100‑1 (MQ=255)
                                                           tGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGtt                                     <  2:1586394/100‑1 (MQ=255)
                                                           tGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGtt                                     >  2:1160749/1‑100 (MQ=255)
                                                           tGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGtt                                     <  2:2264909/100‑1 (MQ=255)
                                                            gCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTa                                    >  1:2144718/1‑100 (MQ=255)
                                                            gCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTa                                    >  2:406700/1‑100 (MQ=255)
                                                            gCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTa                                    >  2:261074/1‑100 (MQ=255)
                                                              aaCGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAag                                  >  2:550634/1‑100 (MQ=255)
                                                               aCGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAagc                                 >  1:1149611/1‑100 (MQ=255)
                                                               aCGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAagc                                 <  1:1031112/100‑1 (MQ=255)
                                                                cGCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAagca                                <  2:2246548/100‑1 (MQ=255)
                                                                 gCCGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAagcag                               <  1:233248/100‑1 (MQ=255)
                                                                  ccGATGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAagcagc                              <  2:565712/100‑1 (MQ=255)
                                                                      tGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCGGCTTCGCCAGTTTGTTCGTTAAGCAGCaata                          <  1:1348485/100‑1 (MQ=255)
                                                                      tGGTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCaata                          <  1:311874/100‑1 (MQ=255)
                                                                       ggTGTCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCaataa                         >  1:1676833/1‑100 (MQ=255)
                                                                         tgtCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGCTATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTCTTCGTTAAGCAGCAATAAtt                       >  1:804330/1‑100 (MQ=255)
                                                                         tgtCTGTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAATTTGTTCGTTAAGCAGCAATAAtt                       <  2:2972009/100‑1 (MQ=255)
                                                                         tgtCTGTAACGCTGGATTGCACACAGGTGGGAATGGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTGCGCCAGTTTGGTCGTTAAGCAGCaataat                        >  2:331074/1‑99 (MQ=255)
                                                                              gTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTAcccc                  <  1:2987163/100‑1 (MQ=255)
                                                                              gTAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTAccc                   <  2:394635/99‑1 (MQ=255)
                                                                               tAACGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCg                 >  1:625263/1‑100 (MQ=255)
                                                                                aaCGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCgg                <  1:2003359/100‑1 (MQ=255)
                                                                                aaCGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCgg                <  2:331987/100‑1 (MQ=255)
                                                                                aaCGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCgg                >  1:1271580/1‑100 (MQ=255)
                                                                                 aCGCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGt               <  1:2778083/100‑1 (MQ=255)
                                                                                   gCTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTg             <  1:257207/100‑1 (MQ=255)
                                                                                    cTGGTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGt            >  1:2659405/1‑100 (MQ=255)
                                                                                       gTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCAc         <  2:1546185/100‑1 (MQ=255)
                                                                                       gTTTCCACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCAc         >  2:2682639/1‑100 (MQ=255)
                                                                                           ccACACCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCgg     >  2:1804212/1‑100 (MQ=255)
                                                                                            cacaCCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGa    >  1:1509999/1‑100 (MQ=255)
                                                                                             acacCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCATTAAGCAGCAATAATTACCCCGGTTGTCACCCGGAt   <  1:2131511/100‑1 (MQ=255)
                                                                                              cacCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGAt   <  1:2803196/99‑1 (MQ=255)
                                                                                              cacCGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATc  <  2:236104/100‑1 (MQ=255)
                                                                                                 |                                                                                                
AGCTGGCTACAGCTCGTTATTGGTGTGCTGGATATCGTCATTGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATC  >  NC_000913/3657397‑3657590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A