Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A9 F255 I0 R1
|
121 |
91.8 |
4500463 |
95.1% |
4279940 |
99.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
3,338,374 |
T→C |
36.9% |
N30D (AAC→GAC) |
mlaD ← |
OM lipid asymmetry maintenance protein; membrane‑anchored ABC family periplasmic binding protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,338,374 | 0 | T | C | 36.9%
| 59.3
/ 78.1
| 80 | N30D (AAC→GAC) | mlaD | OM lipid asymmetry maintenance protein; membrane‑anchored ABC family periplasmic binding protein |
| Reads supporting (aligned to +/- strand): ref base T (25/25); new base C (17/12); total (43/37) |
| Fisher's exact test for biased strand distribution p-value = 4.92e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 |
CACCAATACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATT > NC_000913/3338278‑3338471
|
caccaATACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTCcgc > 1:650661/1‑100 (MQ=255)
ccaATACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTcgccg > 1:2051977/1‑100 (MQ=255)
ccaATACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTcgccg > 1:156330/1‑100 (MQ=255)
aaTACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTcgccg < 1:144732/98‑1 (MQ=255)
aaTACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCgccgcc < 2:1416280/100‑1 (MQ=255)
aaTACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTCCgccgcc > 2:374182/1‑100 (MQ=255)
tACTGACCGGAGAGCGCGCTTTCAGGCCGCCTATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCtt < 2:576591/100‑1 (MQ=255)
aCTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTc < 1:17475/100‑1 (MQ=255)
tGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTCCGCCGCCTTCAg < 1:345546/100‑1 (MQ=255)
naCCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGATCGGTTCAGTCGGTATATACGTCACGTACGACGCCTTCAgg > 2:2057874/2‑100 (MQ=255)
gACTGGAGAGCGCGCTTTCAGGCCGCCAATGTTAGCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAg < 1:1316692/99‑1 (MQ=255)
gACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAgg < 1:1616849/100‑1 (MQ=255)
gACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAg < 1:276487/99‑1 (MQ=255)
aCCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGc > 2:1465541/1‑100 (MQ=255)
gcgcgcTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCaaacaaac > 1:1391976/1‑100 (MQ=255)
gcgcGTTCAGGCCGCCAATGTTAGCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTCCGCCGCCTTCAGGCAaacaaaca < 1:711883/99‑1 (MQ=255)
tttCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACTTTCGCCGCCTTTAGTCAAACAAACAGcgcc < 2:383476/100‑1 (MQ=255)
tttCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTCCGCCGCCTTCAGGCAAACAAACAGcgcc < 2:1104523/100‑1 (MQ=255)
tCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTCCGCCGCCTTCAGGCAAACAAACAGcgccgc < 2:1692365/100‑1 (MQ=255)
tCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATAGACTTAAAATCCGACGCCTTCAGGCAAACAAACAGcgccgc > 2:817375/1‑100 (MQ=255)
ggCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCcag > 1:1285487/1‑100 (MQ=255)
ggCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCcag > 1:1713487/1‑100 (MQ=255)
ggCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCcag > 1:1827045/1‑100 (MQ=255)
ggCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTCCGCCGCCTTCAGGCAAACAAACAGCGCCGCcag > 1:1402565/1‑100 (MQ=255)
ggCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTCCGCCGCCTTCAGGCAAACAAACAACGCCGCcag > 1:576948/1‑100 (MQ=255)
gccgccAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCcagc > 1:1356091/1‑100 (MQ=255)
ccgccAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTCCGCCGTCTTCAGGCAAACAAACAGCGCCGCcagca > 1:87662/1‑100 (MQ=255)
ccAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGtg > 1:5608/1‑100 (MQ=255)
ccAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTCCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGtg > 1:221484/1‑100 (MQ=255)
aaTGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGtgc < 1:289620/99‑1 (MQ=255)
tGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGtgctgc < 2:438250/100‑1 (MQ=255)
gTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTgctgct < 2:2051963/100‑1 (MQ=255)
ttATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTgctgct < 1:776956/99‑1 (MQ=255)
tCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCtaata < 1:290438/100‑1 (MQ=255)
cGAACGTCGCATAAAGCGTGTAGGTCGGTTAAGTACGTATGGACGTAACGTCCGCCGCCCTCAGGCAAACAAACAGCGCCGCCAGCAGTCCTGCtaataa > 2:1071775/1‑100 (MQ=255)
gAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTCCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATaaa > 1:1234448/1‑100 (MQ=255)
gAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTCCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATaaa < 2:1632905/100‑1 (MQ=255)
aCGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATaaaaa < 2:143023/100‑1 (MQ=255)
aCGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTCCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATaaaaa < 1:957821/100‑1 (MQ=255)
cGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTCCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAg > 2:1814487/1‑100 (MQ=255)
cGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTCCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAg < 1:53075/100‑1 (MQ=255)
gTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAATCAGCGCCGCCAGCAGTGCTGCTAATAAAAAGa < 1:2132587/100‑1 (MQ=255)
gTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGa < 2:255045/100‑1 (MQ=255)
cGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTCCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATa > 1:886066/1‑100 (MQ=255)
gCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCGGCAGTGCTGCTAATAAAAAGATac < 2:401742/100‑1 (MQ=255)
gCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATac < 1:1389609/100‑1 (MQ=255)
gCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTCCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATac > 1:308665/1‑100 (MQ=255)
aTAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATaccc < 1:1415347/100‑1 (MQ=255)
aTAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTCCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATaccc > 1:1598030/1‑100 (MQ=255)
aaGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATacccacc < 2:1455490/100‑1 (MQ=255)
gCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTCCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATAcccaccca > 1:81772/1‑100 (MQ=255)
cGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCaa > 2:1055118/1‑100 (MQ=255)
gtAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCaaagt < 2:654113/100‑3 (MQ=255)
tAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAAttt > 2:1773399/1‑100 (MQ=255)
aGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTc > 1:52465/1‑100 (MQ=255)
aGGTCGGTTCAGTACGTATGGACGTCACGTCCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTc < 1:2019883/100‑1 (MQ=255)
aGGTCGGTTCAGTACGTATGGACGTCACGTCCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTc > 2:1883084/1‑100 (MQ=255)
ggTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTCa > 1:2051651/1‑100 (MQ=255)
ggTCGGTTCAGTACGTATGGACGTCACGTCCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTCa > 1:909310/1‑100 (MQ=255)
tCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTCAtt < 1:164336/100‑1 (MQ=255)
ggTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTGAttt > 1:1027512/1‑99 (MQ=255)
ggTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTCAtttt > 1:1700968/1‑100 (MQ=255)
tCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTCAttttttt > 2:185665/1‑100 (MQ=255)
cAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCACCAGTGCTGATAATAAAAAGAAACCCACCCGAATTTCAtttttttt > 1:1333428/1‑100 (MQ=255)
gTACGTATGGACGTCACGTCCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTCATTTTTTTTc > 2:472015/1‑99 (MQ=255)
tACGTATGGACGTCACGTCCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTCATTTTTTTTCGt < 1:1492700/100‑1 (MQ=255)
aCGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATCCCCACCCAAATTTCATTTTTTTTCGtt < 2:1744483/100‑1 (MQ=255)
cGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTCATTTTTTTTCGttt < 1:1391375/100‑1 (MQ=255)
tATGGACGTCACGTTGGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCTCCCAAATTTCATTTTTTTTCGttgg < 2:179113/99‑3 (MQ=255)
tATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTCATTTTTTTTCGTTTGc > 2:1008502/1‑100 (MQ=255)
ggACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTCATTTTTTTTCTTTTGCATg > 2:609558/1‑100 (MQ=255)
ggACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTCATTTTTTTTCGTTTGCAt > 1:771511/1‑99 (MQ=255)
ggACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTCATTTTTTTTCGTTTGCATg > 2:203454/1‑100 (MQ=255)
gACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGa > 2:1498029/1‑100 (MQ=255)
gACGTCACGTCCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGTTGCTAATAAAAAGATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGa < 2:1596079/100‑1 (MQ=255)
cGTCACGTTCGCCGCCTTCAGGCAAACAAACCGCGCCGCCAGCAGTGCTGCTAATAAAAAGAAACCCACCCAAATTACAATTTTTTTCGTTTGCATGaa > 1:112275/1‑99 (MQ=255)
cGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAAc > 2:711562/1‑100 (MQ=255)
cGTCACGTCCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAAc < 1:850499/100‑1 (MQ=255)
cGTCACGTCCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAAc < 2:81772/100‑1 (MQ=255)
gTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAAtt > 1:955522/1‑100 (MQ=255)
|
CACCAATACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATT > NC_000913/3338278‑3338471
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
