Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F256 I1 R1 186 136.5 6958264 92.1% 6408561 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,622,796 T→G L1202R (CTG→CGG)  rhsB → Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,622,7960TG98.4% 330.9 / ‑2.1 126L1202R (CTG→CGG) rhsBRhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base G (58/66);  total (60/66)
Fisher's exact test for biased strand distribution p-value = 2.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01

Only 100 of 132 total aligned reads displayed.

TATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGTATGGATA  >  NC_000913/3622699‑3622892
                                                                                                 |                                                                                                
tatGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGt                                                                                                <  1:1152792/100‑1 (MQ=38)
 atgatgAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTa                                                                                               >  2:2819710/1‑100 (MQ=25)
  tgatgaATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTAt                                                                                              >  1:483/1‑100 (MQ=25)
   cttgaATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTAtt                                                                                             <  1:3132566/98‑1 (MQ=16)
   cttgaATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTAtt                                                                                             <  1:3134399/98‑1 (MQ=16)
    ctgaATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTa                                                                                            <  2:1484966/99‑1 (MQ=18)
    atgaATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTa                                                                                            <  2:1870476/100‑1 (MQ=18)
     tgaATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTTCCGGGGTAGCAGTATGATGAGGAGTCCGGCCGGTATTAc                                                                                           >  1:2437918/1‑100 (MQ=255)
     tgaATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTag                                                                                           >  2:3132616/1‑99 (MQ=18)
     tgaATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTAc                                                                                           <  1:2304328/100‑1 (MQ=18)
     tgaATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTAc                                                                                           <  1:2915103/100‑1 (MQ=18)
      gaATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACa                                                                                          <  1:2991470/100‑1 (MQ=17)
       naTGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACaa                                                                                         >  2:2153291/2‑100 (MQ=18)
          ggggCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCg                                                                                      <  2:573284/100‑1 (MQ=18)
            ggCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGcc                                                                                    >  2:209253/1‑100 (MQ=18)
             gCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCa                                                                                   >  1:391675/1‑100 (MQ=33)
                aCCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCg                                                                                <  2:3046346/100‑1 (MQ=18)
                 ccTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGc                                                                               >  2:2778623/1‑100 (MQ=18)
                   tgctgAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCta                                                                             >  2:1797333/1‑100 (MQ=18)
                    gctgAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCtat                                                                            >  1:1115473/1‑100 (MQ=17)
                     ctgAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCtatt                                                                           <  1:1644296/100‑1 (MQ=17)
                      tgaatgaaGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCtatta                                                                          <  2:439751/100‑1 (MQ=18)
                        aatgaaGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATg                                                                        >  2:1301475/1‑100 (MQ=17)
                         atgaaGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGa                                                                       >  1:729290/1‑100 (MQ=18)
                          tgaaGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGAc                                                                      >  2:1779479/1‑100 (MQ=18)
                          tgaaGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGAc                                                                      >  2:2431440/1‑100 (MQ=18)
                          tgaaGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGAc                                                                      >  2:257362/1‑100 (MQ=18)
                           gaaGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGAcc                                                                     <  2:52267/100‑1 (MQ=18)
                              nagaACCCGCATCAGCTGCAGCAGCTTATCCGGCTGACGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGa                                                                       >  1:64946/2‑95 (MQ=255)
                               agaACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCTGGTATTACAACCGCCACCGCTATTATGACCCGCt                                                                 >  1:2422697/1‑100 (MQ=12)
                                gaACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTg                                                                >  1:3003563/1‑100 (MQ=18)
                                 aaCCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGc                                                               <  2:1493411/100‑1 (MQ=17)
                                   cccGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCa                                                              >  1:521405/1‑99 (MQ=18)
                                   cccGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAg                                                             >  2:245561/1‑100 (MQ=18)
                                   cccGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAg                                                             >  2:1396830/1‑100 (MQ=18)
                                   cccGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAg                                                             <  2:2248647/100‑1 (MQ=18)
                                    ccGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAgg                                                            <  2:819493/100‑1 (MQ=18)
                                     cGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAggg                                                           <  2:2763703/100‑1 (MQ=18)
                                      gCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAgggg                                                          <  2:3081199/100‑1 (MQ=18)
                                       cATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGc                                                         <  2:214638/100‑1 (MQ=18)
                                        cgccccTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCg                                                        <  2:1669491/95‑1 (MQ=255)
                                        aTCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCg                                                        <  2:555142/100‑1 (MQ=18)
                                         tCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGa                                                       >  1:1098279/1‑100 (MQ=18)
                                           aGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGata                                                     <  2:1276519/100‑1 (MQ=18)
                                           aGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGata                                                     <  2:2608377/100‑1 (MQ=17)
                                           aGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGata                                                     <  1:3157931/100‑1 (MQ=18)
                                           aGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGata                                                     <  2:1972624/100‑1 (MQ=18)
                                            gCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGatat                                                    <  1:1091281/100‑1 (MQ=17)
                                               gcagcaGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATc                                                 >  2:52002/1‑100 (MQ=18)
                                               gcagcaGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATc                                                 <  2:2790773/100‑1 (MQ=18)
                                                 agcagcTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCAc                                               >  1:1383869/1‑100 (MQ=18)
                                                 agcagcTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCAc                                               <  1:2411509/100‑1 (MQ=18)
                                                  gcagcTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACt                                              >  1:553256/1‑100 (MQ=18)
                                                  gcagcTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACt                                              >  2:2556751/1‑100 (MQ=18)
                                                   cagcTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTc                                             <  2:1598343/100‑1 (MQ=18)
                                                     gcTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTTCAGGGTCGATATATCAccc                                             >  2:1378654/1‑96 (MQ=11)
                                                     gcTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAg                                           >  2:977268/1‑100 (MQ=18)
                                                     gcTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAg                                           >  1:1261952/1‑100 (MQ=18)
                                                     gcTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAg                                           <  1:231371/100‑1 (MQ=18)
                                                     gcTTATCCGGCTGCCGGGGCAGCAGCATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAg                                           <  1:253344/100‑1 (MQ=12)
                                                      cTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAgg                                          >  2:878501/1‑100 (MQ=17)
                                                         atactgCTGCCCCGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATCTCACTCAGGATc                                       >  1:631432/6‑100 (MQ=11)
                                                         aTCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATc                                       >  2:2197939/1‑100 (MQ=18)
                                                          tCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATcc                                      <  2:2995646/100‑1 (MQ=18)
                                                           ccGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATcc                                      <  2:1730366/99‑1 (MQ=18)
                                                            cGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCg                                     >  1:2040356/1‑99 (MQ=17)
                                                            cGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGa                                    >  1:1946151/1‑100 (MQ=17)
                                                            cGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGa                                    >  2:2634879/1‑100 (MQ=17)
                                                                 nccccggCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGa                               >  2:773696/6‑100 (MQ=2)
                                                                  ccGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGAc                              <  2:1197990/100‑1 (MQ=14)
                                                                    ggggCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTg                            <  1:2662584/100‑1 (MQ=17)
                                                                       gcagcaGTCTGCTGCGGAGTCAGGACGGTATTCCACCCCCCCCTGCTATTATGACCCGCTGCAGGGGCGTTATATCACTCAGGATCCGAGTGGACTGa                           <  1:285619/98‑1 (MQ=2)
                                                                       gcagcaGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAg                         <  1:1309030/100‑1 (MQ=25)
                                                                       gcagcaGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAg                         >  2:2759535/1‑100 (MQ=25)
                                                                        cagcagTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAgg                        >  2:3280845/1‑100 (MQ=35)
                                                                          gcagTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAgggg                      <  1:1712028/100‑1 (MQ=38)
                                                                          gcagTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAgggg                      <  2:2758641/100‑1 (MQ=38)
                                                                          gcagTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAgatc                      >  2:1186855/1‑97 (MQ=255)
                                                                              tATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGAt                  >  2:1347535/1‑100 (MQ=255)
                                                                               atgatgAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATg                 <  2:378951/100‑1 (MQ=25)
                                                                                tgatgaGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATgg                <  2:3477100/100‑1 (MQ=255)
                                                                                 gatgaGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGa               <  2:1610775/100‑1 (MQ=255)
                                                                                  atgaGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGaa              >  1:552494/1‑100 (MQ=25)
                                                                                   tgaGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAAc             <  2:169904/100‑1 (MQ=255)
                                                                                   tgaGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAAc             >  1:2313852/1‑100 (MQ=255)
                                                                                    gaggagTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAAcc            <  2:911666/100‑1 (MQ=255)
                                                                                      ggagTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTg          <  1:1977303/100‑1 (MQ=255)
                                                                                      ggagTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTg          >  2:318183/1‑100 (MQ=255)
                                                                                      ggagTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTg          >  1:2547584/1‑100 (MQ=255)
                                                                                       gagTCCGGCCGGTATTACAACCGCCACCGCTAGTATGACCCGCTGCAGGGGCGATATATCACTCAGCATCCGATTGGACTGAAGGGGGGATGGAACCTGt         <  1:2083744/100‑1 (MQ=255)
                                                                                        agTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGTa        <  2:2173779/100‑1 (MQ=255)
                                                                                         gTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGTAt       <  1:415431/100‑1 (MQ=255)
                                                                                         gTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGTAt       >  2:712840/1‑100 (MQ=255)
                                                                                           ccggccggTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGTATgg     >  2:605467/1‑100 (MQ=255)
                                                                                           ccggccggTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGTATgg     >  2:2210509/1‑100 (MQ=255)
                                                                                           ccggccggTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGTATgg     >  2:3176538/1‑100 (MQ=255)
                                                                                           ccggccggTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGAACTGAAGGGGGGATGGAACCTGTAt       >  1:2843455/1‑98 (MQ=255)
                                                                                            cGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGTATggn    <  1:773685/100‑2 (MQ=255)
                                                                                            cGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGTATGGa    <  1:475418/100‑1 (MQ=255)
                                                                                             ggCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGTATGGat   >  2:365674/1‑100 (MQ=255)
                                                                                                 |                                                                                                
TATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGTATGGATA  >  NC_000913/3622699‑3622892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A