Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F133 I0 R1 200 171.4 8351354 94.3% 7875326 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,622,796 T→G 100% L1202R (CTG→CGG)  rhsB → Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,622,7960TG98.8% 467.4 / ‑2.2 179L1202R (CTG→CGG) rhsBRhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base G (85/91);  total (86/93)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 182 total aligned reads displayed.

ATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGTATGGATA  >  NC_000913/3622700‑3622892
                                                                                                |                                                                                                
atgatgAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTa                                                                                               <  1:3219293/100‑1 (MQ=25)
 tgatgaATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTAt                                                                                              >  1:4087177/1‑100 (MQ=25)
 tgatgaATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTAt                                                                                              >  2:206617/1‑100 (MQ=25)
  gatgaATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTAtt                                                                                             <  2:1631690/100‑1 (MQ=17)
  gatgaATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTAtt                                                                                             >  1:3500799/1‑100 (MQ=18)
  gatgaATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTAtt                                                                                             <  2:2115701/100‑1 (MQ=18)
   atgaATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTa                                                                                            <  2:565156/100‑1 (MQ=18)
   atgaATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTa                                                                                            <  2:425203/100‑1 (MQ=18)
    tgaATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTAc                                                                                           <  2:2277157/100‑1 (MQ=32)
    tgaATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTAc                                                                                           >  2:358237/1‑100 (MQ=17)
     gaATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACa                                                                                          <  1:3658623/100‑1 (MQ=18)
      aaTGGGGCAACCTGCTGAATGAAGAGAACCCGCAGCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGGATGATGAGGAGTCCGGCCGGTATTa                                                                                            <  2:3664407/97‑1 (MQ=255)
         ggggCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCg                                                                                      >  1:3730856/1‑100 (MQ=18)
         ggggCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCg                                                                                      <  1:69028/100‑1 (MQ=18)
          gggCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGc                                                                                     >  2:1726663/1‑100 (MQ=18)
          gggCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGc                                                                                     >  1:3614021/1‑100 (MQ=18)
          gggCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGc                                                                                     >  2:3651280/1‑100 (MQ=17)
           ggCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGcc                                                                                    <  2:2236807/100‑1 (MQ=18)
           ggCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGcc                                                                                    >  1:1333019/1‑100 (MQ=18)
           ggCAACCTGCTGAATGAAGAGAACCCACATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGc                                                                                     <  2:1288645/99‑1 (MQ=16)
            gCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTCTGATGAGGAGTCCGGCCGGTATTACAACCGcc                                                                                    <  1:3959881/99‑1 (MQ=12)
              aaCCTGCTGAATGAAGAGAACCCGTATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCAc                                                                                  <  2:37980/99‑1 (MQ=12)
              aaCCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCAcc                                                                                 <  1:1199440/100‑1 (MQ=18)
                 ctgctgAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCt                                                                              >  1:2031971/1‑100 (MQ=17)
                  tgctgAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCta                                                                             >  2:3057737/1‑100 (MQ=18)
                   gctgAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCtat                                                                            >  2:2951614/1‑100 (MQ=18)
                    ctgAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCtatt                                                                           <  1:2375685/100‑1 (MQ=18)
                     tgaatgaaGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCtatta                                                                          >  1:2323001/1‑100 (MQ=18)
                     tgaatgaaGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGCCCTGTATTACAACCGACACCTCtatta                                                                          >  2:3969820/1‑100 (MQ=255)
                      gaatgaaGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCtattat                                                                         <  2:1712503/100‑1 (MQ=18)
                        atgaaGAGAACCCGCATCAGATGCAGCAGCTTATCCGGATGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGa                                                                       >  2:506717/1‑100 (MQ=255)
                         tgaaGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGAc                                                                      >  1:2774354/1‑100 (MQ=18)
                           aaGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGAccc                                                                    >  1:639235/1‑100 (MQ=18)
                           aaGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGAccc                                                                    >  1:3910381/1‑100 (MQ=18)
                           aaGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGAccc                                                                    >  2:4054618/1‑100 (MQ=17)
                             gagaACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGc                                                                  <  1:2165582/100‑1 (MQ=18)
                             gagaACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGc                                                                  <  2:2282383/100‑1 (MQ=18)
                               gaACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTg                                                                >  2:842867/1‑100 (MQ=17)
                                 aCCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCa                                                              >  1:1797089/1‑100 (MQ=18)
                                     gCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAgggg                                                          <  1:1648184/100‑1 (MQ=17)
                                     gCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCACTGCAgggg                                                          <  2:139717/100‑1 (MQ=255)
                                     gCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGAGGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAgggg                                                          >  1:2383917/1‑100 (MQ=12)
                                       aTCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCg                                                        >  1:3768190/1‑100 (MQ=18)
                                         cAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGat                                                      <  2:1073354/100‑1 (MQ=17)
                                          aGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGata                                                     >  2:2679308/1‑100 (MQ=18)
                                            cTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGatata                                                   <  2:167893/100‑1 (MQ=18)
                                            cTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGatata                                                   >  1:1129057/1‑100 (MQ=18)
                                              gcagcaGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATc                                                 <  1:3182999/100‑1 (MQ=17)
                                               cagcagCTTCTCCGGCTGCCGCGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCTACCGCTATTATGACCCGCTGCAGGGGCGATATATc                                                 <  2:520485/99‑1 (MQ=11)
                                               cagcagCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCa                                                >  2:3375667/1‑100 (MQ=18)
                                                agcagcTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCAc                                               <  2:2993968/100‑1 (MQ=18)
                                                 gcagcTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACt                                              >  2:1901739/1‑100 (MQ=18)
                                                    gcTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAg                                           >  1:917112/1‑100 (MQ=18)
                                                    gcTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAg                                           <  2:1376830/100‑1 (MQ=18)
                                                    gcTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAg                                           >  1:2172986/1‑100 (MQ=18)
                                                    gcTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAg                                           >  1:2244695/1‑100 (MQ=18)
                                                     cTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAgg                                          >  1:1700596/1‑100 (MQ=18)
                                                       tATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGAt                                        <  2:3817021/100‑1 (MQ=18)
                                                       tATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGAt                                        >  1:559139/1‑100 (MQ=18)
                                                        atactgCTGCCCCGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATc                                       >  1:106989/6‑100 (MQ=11)
                                                        aTCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATc                                       <  1:3053077/100‑1 (MQ=18)
                                                         tCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATcc                                      >  2:54759/1‑100 (MQ=17)
                                                         tCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATcc                                      <  1:4135977/100‑1 (MQ=18)
                                                          ccGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCg                                     <  1:1875150/100‑1 (MQ=18)
                                                            tgCTGCCCCGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGAt                                   >  1:3567510/2‑100 (MQ=11)
                                                            ggCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGAt                                   <  1:1596199/100‑1 (MQ=14)
                                                                gCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGa                               >  1:2861702/1‑100 (MQ=11)
                                                                 ccGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACACCCGCCACCGCTATTATCACCCGCTGCAGCGACGATATATCACTAAGGATCCGATTGGa                               >  2:1845599/1‑99 (MQ=11)
                                                                 ccGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGAc                              <  2:1862003/100‑1 (MQ=14)
                                                                 ccGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGAc                              <  2:2182233/100‑1 (MQ=14)
                                                                  cGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACt                             <  1:726844/100‑1 (MQ=17)
                                                                     ggCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGaa                          >  1:1020786/1‑100 (MQ=17)
                                                                     ggCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGaa                          >  1:3449388/1‑100 (MQ=18)
                                                                       cagcagTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAgg                        <  1:2098104/100‑1 (MQ=25)
                                                                       cagcagTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAgg                        >  2:1237463/1‑100 (MQ=25)
                                                                          cagTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAggggg                     <  1:880474/100‑1 (MQ=255)
                                                                          cagTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAggggg                     <  1:2163896/100‑1 (MQ=255)
                                                                          cagTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAggggg                     <  2:2951046/100‑1 (MQ=25)
                                                                           agTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAgggggg                    <  1:1774702/100‑1 (MQ=255)
                                                                           agTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAgggggg                    >  2:1489923/1‑100 (MQ=25)
                                                                            gTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGa                   >  2:1774627/1‑100 (MQ=255)
                                                                             tATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGAt                  <  2:574656/100‑1 (MQ=255)
                                                                              atgatgAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATg                 <  2:2545826/100‑1 (MQ=38)
                                                                              atgatgAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATg                 >  1:3628064/1‑100 (MQ=25)
                                                                               tgatgaGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATgg                >  1:122531/1‑100 (MQ=255)
                                                                                gatgaGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGa               >  1:2192646/1‑100 (MQ=25)
                                                                                 atgaGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGaa              >  1:1444102/1‑100 (MQ=25)
                                                                                  tgaGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAAc             >  1:2526849/1‑100 (MQ=255)
                                                                                  tgaGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAAc             <  2:4143111/100‑1 (MQ=255)
                                                                                   gaggagTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAAcc            <  2:2144027/100‑1 (MQ=255)
                                                                                       agTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGTa        <  1:1306294/100‑1 (MQ=255)
                                                                                       agTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTAAGGATCCGATTGGACTGAAGGGGTGATGGAACCTGTa        >  2:236781/1‑100 (MQ=255)
                                                                                       agTCCCGCCGGGATTCCAACCGCCACCGATATTATGACCCGCTGCAGGGGCGATATATCACACAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGt         <  2:688088/99‑1 (MQ=14)
                                                                                        gTCCGGCCGGTATTACAACCGCCACCGCTATTCTGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGAGATGGAACCTGTAt       >  2:875960/1‑100 (MQ=255)
                                                                                         tCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGTATg      <  1:2642546/100‑1 (MQ=255)
                                                                                          ccggccggTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCCATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGTATgg     >  2:3437978/1‑100 (MQ=255)
                                                                                           cGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGTATgg     <  2:1367726/99‑1 (MQ=255)
                                                                                           cGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGTATg      <  2:1104048/98‑1 (MQ=255)
                                                                                           cGGCCGGGATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGTATg      <  2:1161410/98‑1 (MQ=255)
                                                                                            ggCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGTATGGat   >  1:3091950/1‑100 (MQ=255)
                                                                                                |                                                                                                
ATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGTATGGATA  >  NC_000913/3622700‑3622892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A