breseq  version 0.29.0  revision 8f9c342918e4
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 36,231 T→C intergenic (‑69/+40) caiD ← / ← caiC carnitinyl‑CoA dehydratase/putative crotonobetaine/carnitine‑CoA ligase
RA 53,012 A→G G135G (GGT→GGC pdxA ← 4‑hydroxy‑L‑threonine phosphate dehydrogenase, NAD‑dependent
RA 88,208 A→T T61S (ACA→TCA)  cra → transcriptional repressor‑activator for carbon metabolism
RA 107,553 (T)9→8 intergenic (+79/‑152) lpxC → / → secM UDP‑3‑O‑acyl N‑acetylglucosamine deacetylase/regulator of secA translation
RA 129,360 G→T intergenic (+24/+47) lpd → / ← yacH dihydrolipoyl dehydrogenase; E3 component of pyruvate and 2‑oxoglutarate dehydrogenases complexes; glycine cleavage system L protein; dihydrolipoamide dehydrogenase/DUF3300 family protein
RA 160,736 A→T intergenic (‑132/+46) dksA ← / ← sfsA transcriptional regulator of rRNA transcription; DnaK suppressor protein/sugar fermentation stimulation protein A
RA 162,973 G→A C290Y (TGT→TAT)  hrpB → putative ATP‑dependent helicase
RA 178,902 C→T L84L (TTG→TTA mtn ← 5'‑methylthioadenosine/S‑adenosylhomocysteine nucleosidase
RA 179,327 A→T I31F (ATC→TTC)  dgt → deoxyguanosine triphosphate triphosphohydrolase
RA 179,442 A→T H69L (CAC→CTC)  dgt → deoxyguanosine triphosphate triphosphohydrolase
JC 190,553 Δ14 bp coding (680‑693/726 nt) rpsB → 30S ribosomal subunit protein S2
RA 194,128 C→T P203L (CCG→CTG)  dxr → 1‑deoxy‑D‑xylulose 5‑phosphate reductoisomerase
RA 216,568 C→T A146T (GCC→ACC)  yaeF ← putative lipoprotein
RA 232,196 G→T G91C (GGC→TGC)  yafE → putative S‑adenosyl‑L‑methionine‑dependent methyltransferase
RA 236,541 C→T R159* (CGA→TGA)  dnaQ → DNA polymerase III epsilon subunit
MC JC 257,908 Δ776 bp [crl] [crl]
RA 265,418 A→G R42R (CGT→CGC ykfF ← CP4‑6 prophage; uncharacterized protein
RA 281,581 G→A R135W (CGG→TGG)  yagA ← CP4‑6 prophage; putative DNA‑binding transcriptional regulator
RA 362,884 A→G L99S (TTA→TCA)  lacY ← lactose permease
RA 364,024 C→T R761H (CGC→CAC)  lacZ ← beta‑D‑galactosidase
RA 367,830 G→A Y197Y (TAC→TAT mhpR ← mhp operon transcriptional activator
RA 383,477 G→A G153G (GGC→GGT yaiP ← putative family 2 glycosyltransferase
RA 400,214 T→C D237G (GAC→GGC)  ddlA ← D‑alanine‑D‑alanine ligase A
RA 416,389 (C)5→4 coding (564/1203 nt) sbcD ← exonuclease, dsDNA, ATP‑dependent
RA 428,188 G→T R181L (CGC→CTC)  secD → SecYEG protein translocase auxillary subunit
RA 459,256 T→C D123D (GAT→GAC lon → DNA‑binding ATP‑dependent protease La
RA 459,642 A→G E252G (GAA→GGA)  lon → DNA‑binding ATP‑dependent protease La
RA 497,139 (T)5→6 intergenic (+145/‑36) htpG → / → adk protein refolding molecular co‑chaperone Hsp90, Hsp70‑dependent; heat‑shock protein; ATPase/adenylate kinase
RA 520,993 T→G V193G (GTC→GGC)  ybbP → putative ABC transporter permease
RA 532,380 A→G intergenic (‑159/‑71) allS ← / → allA allD operon transcriptional activator/ureidoglycolate lyase, releasing urea
RA 584,262 (A)7→8 coding (582/750 nt) appY → global transcriptional activator; DLP12 prophage
RA 627,309 G→A G414S (GGC→AGC)  entE → 2,3‑dihydroxybenzoate‑AMP ligase component of enterobactin synthase multienzyme complex
RA 649,964 +G coding (527/909 nt) citE ← citrate lyase, citryl‑ACP lyase (beta) subunit
RA 661,369 T→C N4D (AAT→GAT)  ybeF ← LysR family putative transcriptional regulator
RA 662,636 T→G T3P (ACC→CCC)  ybeD ← UPF0250 family protein
RA 700,805 A→T C264S (TGC→AGC)  nagC ← N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor
RA 712,150 A→T L218Q (CTG→CAG)  ybfF ← acyl‑CoA esterase
RA 720,946 C→T intergenic (+106/+110) ybfK → / ← kdpE uncharacterized protein/response regulator in two‑component regulatory system with KdpD
RA 750,896 Δ1 bp coding (1274/2448 nt) ybgQ ← putative outer membrane protein
RA 772,209 C→A T251N (ACC→AAC)  cydA → cytochrome d terminal oxidase, subunit I
RA 834,352 A→T Q428L (CAG→CTG)  dinG → ATP‑dependent DNA helicase
RA 851,094 G→A P502S (CCG→TCG)  opgE ← OPG biosynthetic transmembrane phosphoethanolamine transferase
RA 882,722 G→A R5C (CGC→TGC)  deoR ← deoxyribose‑5‑phosphate‑inducible deoxyribose operon transcriptional repressor; repressor of nupG and tsx
RA 894,155 T→G D124E (GAT→GAG potF → putrescine ABC transporter periplasmic binding protein
RA 905,720 C→A L270M (CTG→ATG)  amiD → 1,6‑anhydro‑N‑acetylmuramyl‑L‑alanine amidase, Zn‑dependent; OM lipoprotein
RA 931,952 C→T V33V (GTG→GTA trxB ← thioredoxin reductase, FAD/NAD(P)‑binding
RA 933,387 C→T A55V (GCC→GTC)  ftsK → DNA translocase at septal ring sorting daughter chromsomes
RA 966,368 A→G A683A (GCA→GCG ycaI → ComEC family inner membrane protein
RA 975,083 T→A D255E (GAT→GAA mukF → chromosome condensin MukBEF, kleisin‑like subunit, binds calcium
RA 1,017,296 A→C I335S (ATC→AGC)  ycbZ ← putative peptidase
RA 1,065,426 A→T intergenic (+134/‑159) yccE → / → agp uncharacterized protein/glucose‑1‑phosphatase/inositol phosphatase
RA 1,068,621 T→A T407S (ACG→TCG)  rutG ← pyrimidine permease
RA 1,073,307 Δ1 bp coding (705/1149 nt) rutA ← pyrimidine oxygenase, FMN‑dependent
RA 1,097,209 T→G intergenic (+380/+356) ycdU → / ← serX putative inner membrane protein/tRNA‑Ser
RA 1,100,233 G→A intergenic (+39/‑63) ycdY → / → ycdZ redox enzyme maturation protein (REMP) chaperone for YcdX/DUF1097 family inner membrane protein
RA 1,111,377 A→G D172G (GAT→GGT)  opgH → OPG biosynthetic ACP‑dependent transmembrane UDP‑glucose beta‑1,2 glycosyltransferase; nutrient‑dependent cell size regulator, FtsZ assembly antagonist
RA 1,143,125 C→T D415N (GAC→AAC)  rne ← endoribonuclease; RNA‑binding protein;RNA degradosome binding protein
RA 1,170,338 (A)6→5 coding (37/963 nt) ldtC ← L,D‑transpeptidase linking Lpp to murein
RA 1,197,340 T→C T65A (ACT→GCT)  ymfD ← e14 prophage; putative SAM‑dependent methyltransferase
RA 1,197,739 A→G S167P (TCC→CCC)  ymfE ← e14 prophage; putative inner membrane protein
RA 1,207,981 G→A A161T (GCC→ACC)  stfP → e14 prophage; uncharacterized protein
RA 1,292,944 T→C intergenic (‑22/‑583) hns ← / → tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
JC JC 1,293,196 IS5 (+) +4 bp intergenic (‑274/‑328) hns ← / → tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
RA 1,307,634 T→A I338F (ATC→TTC)  clsA ← cardiolipin synthase 1
RA 1,369,793 G→A E35E (GAG→GAA pspE → thiosulfate:cyanide sulfurtransferase (rhodanese)
RA 1,382,820 T→C Y292H (TAT→CAT)  ompG → outer membrane porin G
RA 1,399,677 C→T intergenic (‑151/+44) fnr ← / ← ogt oxygen‑sensing anaerobic growth regulon transcriptional regulator FNR; autorepressor/O‑6‑alkylguanine‑DNA:cysteine‑protein methyltransferase
RA 1,401,844 A→G I471T (ATC→ACC)  abgB ← p‑aminobenzoyl‑glutamate hydrolase, B subunit
RA 1,407,859 A→G intergenic (‑64/‑191) ydaM ← / → ydaN diguanylate cyclase, csgD regulator/putative Zn(II) transporter
RA 1,433,718 T→C intergenic (‑44/+273) pinR ← / ← ynaE Rac prophage; putative site‑specific recombinase/cold shock protein, Rac prophage
RA 1,434,459 C→T intergenic (‑235/+499) ynaE ← / ← ttcC cold shock protein, Rac prophage/pseudogene, prophage Rac integration site ttcA duplication;Phage or Prophage Related
RA 1,435,737 G→A intergenic (‑118/+23) uspF ← / ← ompN stress‑induced protein, ATP‑binding protein/outer membrane pore protein N, non‑specific
RA 1,448,947 A→C T477P (ACG→CCG)  feaB → phenylacetaldehyde dehydrogenase
RA 1,470,558 G→A pseudogene (42/3497 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,493,774 A→G L435L (TTA→TTG trg → methyl‑accepting chemotaxis protein III, ribose and galactose sensor receptor
RA 1,501,912 T→A I117I (ATT→ATA tehB → tellurite, selenium methyltransferase, SAM‑dependent; tellurite, selenium resistance protein
RA 1,530,076 (A)7→8 coding (155/483 nt) ydcD → putative immunity protein for RhsE
RA 1,547,395 A→G intergenic (‑226/‑6) yddG ← / → fdnG aromatic amino acid exporter/formate dehydrogenase‑N, alpha subunit, nitrate‑inducible
RA 1,555,324 A→G S116P (TCT→CCT)  maeA ← malate dehydrogenase, decarboxylating, NAD‑requiring; malic enzyme
RA 1,580,094 C→T D233N (GAT→AAT)  ydeM ← putative YdeN‑specific sulfatase‑maturating enzyme
RA 1,650,904 T→C pseudogene (60/693 nt) insD1 → IS2 transposase TnpB;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,658,592 C→T A175V (GCC→GTC)  ynfE → putative selenate reductase, periplasmic
RA 1,665,195 (T)7→8 intergenic (+75/‑120) dmsD → / → clcB twin‑argninine leader‑binding protein for DmsA and TorA/H(+)/Cl(‑) exchange transporter
RA 1,681,932 T→C P33P (CCA→CCG ydgC ← GlpM family inner membrane protein
RA 1,707,029 A→G V37V (GTA→GTG rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,712,306 (T)7→8 intergenic (+148/‑463) nth → / → dtpA DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III)/dipeptide and tripeptide permease A
RA 1,712,518 (C)6→5 intergenic (+360/‑251) nth → / → dtpA DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III)/dipeptide and tripeptide permease A
RA 1,728,444 C→A T33N (ACC→AAC)  rnt → RNase T; exoribonuclease T; structured DNA 3' exonuclease; RNA processing; DNA repair
RA 1,827,031 T→A D410V (GAT→GTT)  astB ← succinylarginine dihydrolase
RA 1,880,549 T→C T71A (ACC→GCC)  leuE ← leucine efflux protein
RA 1,881,128 C→A E228* (GAA→TAA)  dmlR ← transcriptional activator of dmlA
RA 1,946,790 T→G G180G (GGT→GGG yebB → DUF830 family protein
RA 1,971,761 T→C T311A (ACC→GCC)  tar ← methyl‑accepting chemotaxis protein II
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
RA 1,983,761 C→T E437K (GAA→AAA)  araG ← L‑arabinose ABC transporter ATPase
RA 2,071,321 A→G intergenic (+110/‑218) yeeP → / → flu pseudogene, CP4‑44 prophage; 50S ribosome‑binding GTPase family;Phage or Prophage Related; putative histone/CP4‑44 prophage; antigen 43 (Ag43) phase‑variable biofilm formation autotransporter
RA 2,159,354 C→T R323R (CGC→CGT mdtC → multidrug efflux system, subunit C
RA 2,161,946 A→G A161A (GCA→GCG iceT → putative citrate/iron‑citrate/zinc‑citrate efflux transporter
RA 2,173,076 C→A pseudogene (287/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,191,432 C→T G72S (GGC→AGC)  yehC ← putative periplasmic pilin chaperone
RA 2,197,365 G→A pseudogene (892/2847 nt) yehH → DUF4132 family pseudogene; defective yehI paralog; molybdate metabolism regulator, first fragment; molybdate metabolism regulator, second fragment 2; yehI' molR mutations have phenotypes and the N‑terminal molR fragment of yehI' may be expressed and may have retained or evolved MolR function
RA 2,210,811 C→T pseudogene (1712/2001 nt) yehQ → pseudogene
RA 2,235,656 T→C I131T (ATC→ACC)  preA → dihydropyrimidine dehydrogenase, NADH‑dependent, subunit C
RA 2,246,800 A→G intergenic (‑31/+263) cirA ← / ← lysP colicin IA outer membrane receptor and translocator; ferric iron‑catecholate transporter/lysine transporter
RA 2,250,210 T→A V165E (GTG→GAG)  yeiH → UPF0324 family inner membrane protein
RA 2,278,298 C→T intergenic (‑61/+272) yejG ← / ← bcr uncharacterized protein/bicyclomycin/cysteine/sulfonamide efflux transporter
RA 2,279,021 G→A A247V (GCG→GTG)  bcr ← bicyclomycin/cysteine/sulfonamide efflux transporter
RA 2,307,386 A→T D410E (GAT→GAA yojI ← microcin J25 efflux ABC transporter permease/ATPase
RA 2,361,323 T→A Q31L (CAG→CTG)  rhmD ← L‑rhamnonate dehydratase
RA 2,386,365 T→A F169Y (TTC→TAC)  yfbL → putative M28A family peptidase
RA 2,411,637 Δ1 bp coding (453/651 nt) yfbT ← sugar phosphatas
RA 2,420,727 G→A G36D (GGC→GAC)  yfcG → GSH‑dependent disulfide bond oxidoreductase
RA 2,436,937 A→T D305E (GAT→GAA pdxB ← erythronate‑4‑phosphate dehydrogenase
RA 2,469,656 (T)7→8 coding (526/1332 nt) gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
RA 2,493,645 G→A intergenic (‑391/+122) frc ← / ← yfdX formyl‑CoA transferase, NAD(P)‑binding/uncharacterized protein
RA 2,495,173 T→A A40A (GCA→GCT yfdY ← DUF2545 family putative inner membrane protein
RA 2,521,967 C→A A171S (GCG→TCG)  xapR ← transcriptional activator of xapAB
RA 2,525,135 (A)6→5 coding (9/765 nt) yfeN → putative outer membrane protein
RA 2,530,925 T→C Q103Q (CAA→CAG zipA ← FtsZ stabilizer
RA 2,534,206 T→G R47R (CGT→CGG ptsI → PEP‑protein phosphotransferase of PTS system (enzyme I)
RA 2,541,456 A→G I75T (ATT→ACT)  cysW ← sulfate/thiosulfate ABC transporter permease
RA 2,563,518 (A)5→6 intergenic (+401/‑74) yffP → / → yffQ CPZ‑55 prophage; uncharacterized protein/CPZ‑55 prophage; uncharacterized protein
RA 2,564,525 G→A M1M (ATG→ATA) † yffS → CPZ‑55 prophage; uncharacterized protein
RA 2,593,917 G→A A648V (GCG→GTG)  tmcA ← elongator methionine tRNA (ac4C34) acetyltransferase
RA 2,600,288 C→T S128L (TCG→TTG)  gcvR → transcriptional repressor, regulatory protein accessory to GcvA
RA 2,638,037 C→A A206S (GCG→TCG)  bamB ← BamABCDE complex OM biogenesis lipoprotein
RA 2,644,169 G→T P39T (CCC→ACC)  rlmN ← dual specificity 23S rRNA m(2)A2503, tRNA m(2)A37 methyltransferase, SAM‑dependent
RA 2,758,577 G→T N8K (AAC→AAA yfjH ← CP4‑57 prophage; uncharacterized protein
RA 2,777,363 T→C F83F (TTT→TTC yfjZ → CP4‑57 prophage; antitoxin of the YpjF‑YfjZ toxin‑antitoxin system
RA 2,810,756 G→A intergenic (+77/‑14) ygaH → / → mprA putative L‑valine exporter, norvaline resistance protein/transcriptional repressor of microcin B17 synthesis and multidrug efflux
RA 2,852,356 T→A L74Q (CTG→CAG)  hypD → hydrogenase maturation protein
RA 2,867,164 C→T A130T (GCG→ACG)  rpoS ← RNA polymerase, sigma S (sigma 38) factor
RA 2,887,352 T→A intergenic (‑133/+226) ygcB ← / ← cysH Cascade complex anti‑viral R‑loop helicase‑annealase Cas3/phosphoadenosine phosphosulfate reductase; PAPS reductase, thioredoxin dependent
RA 2,900,481 G→T intergenic (‑208/‑111) ygcW ← / → yqcE putative SDR family oxidoreductase/putative MFS transporter, inner membrane protein
RA 2,966,414 T→G D674A (GAT→GCT)  ptsP ← PEP‑protein phosphotransferase enzyme I; GAF domain containing protein
RA 3,041,028 G→A R15C (CGT→TGT)  sdhE ← flavinator of succinate dehydrogenase; antitoxin of CptAB toxin‑antitoxin pair
RA 3,150,120 T→C F153F (TTT→TTC yghA → putative oxidoreductase
RA 3,165,015 T→A D320V (GAT→GTT)  parC ← DNA topoisomerase IV, subunit A
RA 3,350,648 T→A intergenic (‑189/+41) elbB ← / ← arcB isoprenoid biosynthesis protein with amidotransferase‑like domain/aerobic respiration control sensor histidine protein kinase, cognate to two‑component response regulators ArcA and RssB
RA 3,443,086 T→A N338Y (AAC→TAC)  secY ← preprotein translocase membrane subunit
RA 3,452,057 A→T I77N (ATC→AAC)  rplD ← 50S ribosomal subunit protein L4
RA 3,477,434 T→C intergenic (‑15/‑206) fkpA ← / → slyX FKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)/phi X174 lysis protein
RA 3,524,413 A→G I515V (ATT→GTT)  mrcA → penicillin‑binding protein 1a, murein transglycosylase and transpeptidase
RA 3,541,962 T→G I519S (ATC→AGC)  feoB → ferrous iron transporter protein B and GTP‑binding protein; membrane protein
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,597,846 (T)5→4 intergenic (‑286/‑138) livK ← / → panM leucine transporter subunit/PanD autocleavage accelerator, panothenate synthesis
RA 3,622,796 T→G L1202R (CTG→CGG)  rhsB → Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
RA 3,648,042 T→C intergenic (‑254/‑486) dinQ ← / → arsR UV‑inducible membrane toxin, DinQ‑AgrB type I toxin‑antitoxin system/arsenical resistance operon transcriptional repressor; autorepressor
RA 3,669,340 A→G intergenic (‑152/‑252) yhjA ← / → treF putative cytochrome C peroxidase/cytoplasmic trehalase
RA 3,669,920 A→G E110G (GAA→GGA)  treF → cytoplasmic trehalase
RA 3,708,410 T→A intergenic (‑705/+206) dppA ← / ← proK dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor/tRNA‑Pro
RA 3,746,162 G→T L23L (CTG→CTT yiaO → 2,3‑diketo‑L‑gulonate‑binding periplasmic protein
RA 3,770,087 C→A pseudogene (55/114 nt) yibU → pseudogene, H repeat‑associated protein
RA 3,805,475 T→C N159S (AAT→AGT)  waaP ← kinase that phosphorylates core heptose of lipopolysaccharide
RA 3,822,694 G→T G99W (GGG→TGG)  spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA 3,840,547 T→C intergenic (+39/+2) yicS → / ← nepI putative periplasmic protein/putative transporter
RA 3,853,909 A→G intergenic (+267/‑13) tisB → / → emrD toxic membrane persister formation peptide, LexA‑regulated/multidrug efflux system protein
RA 3,906,597 C→A intergenic (‑30/+256) bglG ← / ← phoU transcriptional antiterminator of the bgl operon/negative regulator of PhoR/PhoB two‑component regulator
RA 3,910,662 A→T D288E (GAT→GAA pstS ← phosphate ABC transporter periplasmic binding protein
RA 3,927,735 Δ1 bp coding (581/993 nt) asnA → asparagine synthetase A
RA 3,950,468 G→T intergenic (+48/‑39) ilvL → / → ilvX ilvG operon leader peptide/uncharacterized protein
RA 3,971,690 A→C Q144P (CAG→CCG)  wecC → UDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase
RA 4,017,924 G→A D198N (GAC→AAC)  rmuC → DNA recombination protein
RA 4,098,512 C→A R259R (CGC→CGA rhaS → transcriptional activator of rhaBAD and rhaT
RA 4,152,760 G→T N113K (AAC→AAA ppc ← phosphoenolpyruvate carboxylase
RA 4,168,540 T→A intergenic (+93/‑101) gltT → / → rrlB tRNA‑Glu/23S ribosomal RNA of rrnB operon
RA 4,178,296 A→T intergenic (+8/‑151) nusG → / → rplK transcription termination factor/50S ribosomal subunit protein L11
RA 4,235,689 +A intergenic (+282/‑217) pgi → / → yjbE glucosephosphate isomerase/extracellular polysaccharide production threonine‑rich protein
RA 4,238,726 A→G I365V (ATT→GTT)  yjbH → DUF940 family extracellular polysaccharide protein
MC JC 4,242,942 Δ66 bp coding (510‑575/891 nt) malG ← maltose transporter subunit
RA 4,279,370 G→T A298S (GCA→TCA)  ghxP → guanine/hypoxanthine permease, high affinity; guanine/hypoxanthine:H+ symporter
RA 4,289,526 A→G K94K (AAA→AAG nrfB → nitrite reductase, formate‑dependent, penta‑heme cytochrome c
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,340,287 G→A L79F (CTT→TTT)  adiA ← arginine decarboxylase
RA 4,352,460 T→G intergenic (+103/‑124) yjdJ → / → ghoS GNAT family putative N‑acetyltransferase/antitoxin of GhoTS toxin‑antitoxin pair; endonuclease for ghoT mRNA
RA 4,358,482 T→A I46F (ATC→TTC)  cadA ← lysine decarboxylase, acid‑inducible
RA 4,360,814 T→G Y374S (TAC→TCC)  cadC ← cadBA operon transcriptional activator
JC 4,370,611 Δ5 bp intergenic (‑199/‑73) yjeH ← / → groS putative transporter/Cpn10 chaperonin GroES, small subunit of GroESL
RA 4,478,879 T→C V136A (GTT→GCT)  rraB → protein inhibitor of RNase E
RA 4,490,055 T→G E3D (GAA→GAC yjgR ← DUF853 family protein with NTPase fold
RA 4,500,814 Δ1 bp intergenic (‑23/‑446) yjgX ← / → yjgZ pseudogene fragment/uncharacterized protein
RA 4,547,319 C→A R742S (CGT→AGT)  fimD → fimbrial usher outer membrane porin protein; FimCD chaperone‑usher
RA 4,615,859 T→G T403P (ACC→CCC)  yjjI ← DUF3029 family protein, putative glycine radical enzyme
RA 4,627,837 A→T I175F (ATC→TTC)  nadR → trifunctional protein: nicotinamide mononucleotide adenylyltransferase, ribosylnicotinamide kinase, transcriptional repressor
RA 4,631,700 T→C L323P (CTT→CCT)  slt → lytic murein transglycosylase, soluble

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423705–3424234 3424607–3424238 5–903 81 [79] [79] 80 [rrfD]–[rrlD] [rrfD], [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)183 (1.400) 107/190 0.0 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein