Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F256 I1 R1	186	136.5	6958264	92.1%	6408561	99.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,910,662	A→T	D288E (GAT→GAA)	pstS ←	phosphate ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,910,662	0	A	T	99.2%	406.2 / ‑4.4	124	D288E (GAT→GAA)	pstS	phosphate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand): ref base A (1/0); new base T (61/61); total (63/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.33e-01

Only 100 of 124 total aligned reads displayed.

GCTGGCGTAATCCAGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGATCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGAAGGTTTTGCTCCAGTCT  >  NC_000913/3910566‑3910758

gCTGGCGTAATCCAGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCt                                                                                               >  2:2718737/1‑100 (MQ=255)

gCTGGCGTAATCCAGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCt                                                                                               >  1:1341440/1‑100 (MQ=255)

   ggCGTAATCCAGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTtg                                                                                            >  2:1870330/1‑100 (MQ=255)

   ggCGTAATCCAGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTtg                                                                                            >  1:3472450/1‑100 (MQ=255)

    gCGTAATCCAGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTtgt                                                                                           <  2:1412354/100‑1 (MQ=255)

    gCGTAATCCAGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGGGCTTtg                                                                                            >  2:2771724/1‑99 (MQ=255)

     cGTAATCCAGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTtgtg                                                                                          <  2:340567/100‑1 (MQ=255)

     cGTAATCCAGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTtgtg                                                                                          <  2:2370379/100‑1 (MQ=255)

      gTAATCCAGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTgg                                                                                         <  2:2803288/100‑1 (MQ=255)

       tAATCCAGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGa                                                                                        <  2:1322763/100‑1 (MQ=255)

        aaTCCAGGTCGTTCGCCTGTTTCGCCCCGGTCTTGTATGCCCAGTCGTAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGAt                                                                                       <  1:2347039/100‑1 (MQ=255)

        aaTCCAGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGAt                                                                                       <  1:1688045/100‑1 (MQ=255)

           ccAGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAg                                                                                    >  2:3077552/1‑100 (MQ=255)

           ccAGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAg                                                                                    >  1:2136100/1‑100 (MQ=255)

           ccAGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTCCTGCTTTCTTCTGTTCTTTGTGGATc                                                                                      >  1:1494103/1‑98 (MQ=255)

            cAGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGa                                                                                   >  2:1339462/1‑100 (MQ=255)

             aGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGaa                                                                                  >  2:2467175/1‑100 (MQ=255)

             aGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGaa                                                                                  >  1:3030766/1‑100 (MQ=255)

             aGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGaa                                                                                  >  1:298120/1‑100 (MQ=255)

              ggTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAAt                                                                                 >  1:2586376/1‑100 (MQ=255)

                 cGTTCGCCTGTTTCGCCCCGGATTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGATCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGaa                                                                              >  1:1575947/1‑100 (MQ=255)

                 cGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGaa                                                                              >  2:949333/1‑100 (MQ=255)

                  gTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAAc                                                                             <  1:540760/100‑1 (MQ=255)

                   ttCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACg                                                                            <  2:1347793/100‑1 (MQ=255)

                    tCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGt                                                                           >  1:2369539/1‑100 (MQ=255)

                     cGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTg                                                                          >  1:2413605/1‑100 (MQ=255)

                     cGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTg                                                                          <  1:1878160/100‑1 (MQ=255)

                     cGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTg                                                                          <  2:895268/100‑1 (MQ=255)

                      gCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTgg                                                                         >  2:1009977/1‑100 (MQ=255)

                      gCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTgg                                                                         >  2:1857004/1‑100 (MQ=255)

                         tGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTag                                                                      >  1:2296062/1‑100 (MQ=255)

                          gTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTaga                                                                     <  2:2613567/100‑1 (MQ=255)

                             tAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGt                                                                  <  2:1763571/100‑1 (MQ=255)

                             tAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCCGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGt                                                                  >  1:1262622/1‑100 (MQ=255)

                              aGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTa                                                                 >  1:779154/1‑100 (MQ=255)

                                ccccGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAAt                                                               >  1:339541/1‑100 (MQ=255)

                                ccccGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAAt                                                               >  1:2476830/1‑100 (MQ=255)

                                 cccGGTTTTGTACGTCCAGTCGAAGAATTTCAGCACTGCTGTGCATTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAAt                                                               <  1:2489076/99‑1 (MQ=255)

                                 cccGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATa                                                              >  1:2210959/1‑100 (MQ=255)

                                  ccGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAg                                                             >  1:81868/1‑100 (MQ=255)

                                   cGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAgg                                                            <  1:762996/100‑1 (MQ=255)

                                      ttttGTACGCCCAGTCGAAGAATTTCAGCACTTCGGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCa                                                         <  1:1441436/100‑1 (MQ=255)

                                        ttGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATg                                                       <  1:2866488/100‑1 (MQ=255)

                                         tGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGc                                                      >  2:1782396/1‑100 (MQ=255)

                                          gTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCa                                                     >  2:2802937/1‑100 (MQ=255)

                                              gCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCtt                                                 <  2:2008790/100‑1 (MQ=255)

                                               cccAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTc                                                >  2:1235068/1‑100 (MQ=255)

                                                ccAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCg                                               <  2:1202183/100‑1 (MQ=255)

                                                ccAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCg                                               <  1:551830/100‑1 (MQ=255)

                                                   gTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCt                                            >  1:179514/1‑100 (MQ=255)

                                                    tCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCtt                                           <  2:657899/100‑1 (MQ=255)

                                                      gaagaaTTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTc                                         >  2:906820/1‑100 (MQ=255)

                                                      gaagaaTTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTc                                         <  2:2557055/100‑1 (MQ=255)

                                                      gaagaaTTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTc                                         >  1:3243263/1‑100 (MQ=255)

                                                       aagaaTTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCt                                        <  1:2738661/100‑1 (MQ=255)

                                                       aagaaTTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCt                                        <  1:1421942/100‑1 (MQ=255)

                                                       aagaaTTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCt                                        <  1:1475235/100‑1 (MQ=255)

                                                        agaaTTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCtg                                       >  1:959615/1‑100 (MQ=255)

                                                         gaaTTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCtg                                       <  1:451196/99‑1 (MQ=255)

                                                          aaTTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCtggt                                     >  2:1708098/1‑100 (MQ=255)

                                                           aTTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCtggtt                                    >  2:1924001/1‑100 (MQ=255)

                                                           aTTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCtggtt                                    <  1:1582417/100‑1 (MQ=255)

                                                             ttCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCtggttgg                                  >  2:496083/1‑100 (MQ=255)

                                                             ttCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCtggttgg                                  <  1:203667/100‑1 (MQ=255)

                                                              tCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCtggttggt                                 >  1:111040/1‑100 (MQ=255)

                                                               cAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTc                                <  2:904852/100‑1 (MQ=255)

                                                               cAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTc                                <  2:378665/100‑1 (MQ=255)

                                                                aGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCa                               >  1:3357880/1‑100 (MQ=255)

                                                                 gCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAg                              >  2:19752/1‑100 (MQ=255)

                                                                   aCTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGAt                            >  2:2526462/1‑100 (MQ=255)

                                                                    cTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATc                           >  2:772399/1‑100 (MQ=255)

                                                                     ttCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATcc                          <  2:2739848/100‑1 (MQ=255)

                                                                     ttCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGAGCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTTGTCAGATcc                          <  1:3042125/100‑1 (MQ=255)

                                                                      tCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCt                         <  2:2727514/100‑1 (MQ=255)

                                                                       cTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTg                        <  2:866594/100‑1 (MQ=255)

                                                                       cTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTg                        <  1:2718694/100‑1 (MQ=255)

                                                                        tgtgCCTTGTTCTGGTTTCTTCTGATCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGa                       >  1:25870/1‑100 (MQ=255)

                                                                         gtgCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAg                      <  2:3463716/100‑1 (MQ=255)

                                                                         gtgCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAg                      >  1:2283664/1‑100 (MQ=255)

                                                                            ccTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGa                   >  1:352744/1‑100 (MQ=255)

                                                                             cTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGaa                  >  1:2482866/1‑100 (MQ=255)

                                                                              ttGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGAAg                 <  1:2958376/100‑1 (MQ=255)

                                                                               tGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGAAgg                <  1:2449285/100‑1 (MQ=255)

                                                                                gTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGAAGGt               >  1:782629/1‑100 (MQ=255)

                                                                                  tCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGAAGGttt             >  2:1402494/1‑100 (MQ=255)

                                                                                  tCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGAAGGttt             >  1:2866220/1‑100 (MQ=255)

                                                                                   cTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGACTTTCTGGTTGGTCAGATCCTGAGCGAAGAtttt            >  1:2942212/1‑100 (MQ=255)

                                                                                    tGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGAAGGtttgc           <  1:1668274/100‑3 (MQ=255)

                                                                                     ggTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGAAGGTTTTGc          >  1:72938/1‑100 (MQ=255)

                                                                                     ggTTTCTTCTGTTCTCTGTGGATCAGAATGAACGGGGGAGAGGTAATAGGCCATGCACCTTCGCCACTCTGGTTGGTCAGATCCTGAGCGAAGGtttt            >  1:1226779/1‑98 (MQ=255)

                                                                                      gTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGAAGGTTTTGCt         <  2:2745684/100‑1 (MQ=255)

                                                                                      gTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGAAGGTTTTGCt         <  1:2164507/100‑1 (MQ=255)

                                                                                        ttcttcTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGAAGGTTTTGCTcc       <  1:248676/100‑1 (MQ=255)

                                                                                        ttcttcTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGAAGGTTTTGCTcc       >  2:700394/1‑100 (MQ=255)

                                                                                        atcttctGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGAAGGTTTTGCTcc       <  2:1078238/99‑1 (MQ=255)

                                                                                            tctGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGAAGGTTTTGCTCCAgat   >  1:1078225/1‑98 (MQ=255)

                                                                                            tctGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGAAGGTTTTGCTCCAGTc   >  2:2485119/1‑100 (MQ=255)

                                                                                            tctGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGAAGGTTTTGCTCCAGTc   <  1:546410/100‑1 (MQ=255)

                                                                                            tctGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGAAGGGTTTGCTCCAGTc   >  2:1514599/1‑100 (MQ=255)

                                                                                             ctGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGAAGGTTTTGCTCCAGTCt  >  1:1993698/1‑100 (MQ=255)

GCTGGCGTAATCCAGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGATCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGAAGGTTTTGCTCCAGTCT  >  NC_000913/3910566‑3910758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A