Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F56 I0 R1	312	170.3	8276235	93.7%	7754832	99.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,910,662	A→T	100%	D288E (GAT→GAA)	pstS ←	phosphate ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,910,662	0	A	T	98.5%	652.6 / ‑0.9	194	D288E (GAT→GAA)	pstS	phosphate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand): ref base A (2/1); new base T (80/111); total (82/112)
Fisher's exact test for biased strand distribution p-value = 5.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

Only 100 of 194 total aligned reads displayed.

GGCTGGCGTAATCCAGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGATCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGAAGGTTTTGCTCCAGTCT  >  NC_000913/3910565‑3910758

  cTGGCGTAATCCAGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCtt                                                                                              >  1:2010905/1‑100 (MQ=255)

    ggCGTAATCCAGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCCCTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCttt                                                                                             >  2:891485/1‑99 (MQ=255)

    ggCGTAATCCAGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTtg                                                                                            <  1:156839/100‑1 (MQ=255)

    ggCGTAATCCAGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTtg                                                                                            >  2:2222471/1‑100 (MQ=255)

     gCGTAATCCAGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTtg                                                                                            <  2:703240/99‑1 (MQ=255)

      cGTAATCCAGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTtgtg                                                                                          <  2:1652054/100‑1 (MQ=255)

      cGTAATCCAGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTtgtg                                                                                          <  2:1004673/100‑1 (MQ=255)

      cGTAATCCAGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTtgtg                                                                                          <  2:3325705/100‑1 (MQ=255)

       gTAATCCAGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTgg                                                                                         >  2:3084021/1‑100 (MQ=255)

         aaTCCAGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGAt                                                                                       <  2:3638526/100‑1 (MQ=255)

          aTCCGGGGCGTTCGCCTCTTCAGCCCCGCTTTCCTACCCCACGTCGCCGGCTCTCAGCACTTCTGTGCCTTCCCCTGGCTTCTTCTCTCCTTTGTGGacc                                                                                      >  2:3685878/1‑98 (MQ=255)

          aTCCAGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATc                                                                                      >  1:52893/1‑100 (MQ=255)

          aTCCAGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTGCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATc                                                                                      <  2:158993/100‑1 (MQ=255)

            ccAGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAg                                                                                    >  1:973454/1‑100 (MQ=255)

            ccAGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAg                                                                                    <  1:2623581/100‑1 (MQ=255)

              aGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGACTTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGaa                                                                                  <  2:1848130/100‑1 (MQ=255)

               ggTCGTTCGCCTGTTTAGCCCCGGTTTTGGACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAAt                                                                                 <  2:969791/100‑1 (MQ=255)

                gTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATg                                                                                <  1:3784324/100‑1 (MQ=255)

                gTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATg                                                                                <  1:3070431/100‑1 (MQ=255)

                     tCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGt                                                                           >  1:3553222/1‑100 (MQ=255)

                      cGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTg                                                                          >  1:2775687/1‑100 (MQ=255)

                       gCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGACCGTg                                                                          >  2:130560/1‑99 (MQ=255)

                       gCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTgg                                                                         <  2:1667522/100‑1 (MQ=255)

                       gCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTgg                                                                         >  2:2037556/1‑100 (MQ=255)

                        ccTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGCACGTGGt                                                                        >  2:3196879/1‑100 (MQ=255)

                        ccTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTgg                                                                         >  1:581509/1‑99 (MQ=255)

                            tttAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTagag                                                                    <  1:303623/100‑1 (MQ=255)

                             ttAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAgg                                                                   >  2:3660883/1‑100 (MQ=255)

                              tAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGt                                                                  <  1:872252/100‑1 (MQ=255)

                              tAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGt                                                                  <  2:1116363/100‑1 (MQ=255)

                              tAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGt                                                                  <  1:317569/100‑1 (MQ=255)

                               aGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTa                                                                 <  2:3946230/100‑1 (MQ=255)

                                gCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTaa                                                                >  2:112249/1‑100 (MQ=255)

                                gCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTaa                                                                >  1:465819/1‑100 (MQ=255)

                                 ccccGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAAt                                                               >  1:3080049/1‑100 (MQ=255)

                                 ccccGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGATCTTTGTGGATCAGAATGAACGTGGTAGAGGTAAt                                                               >  1:924212/1‑100 (MQ=255)

                                  cccGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATa                                                              >  1:1224568/1‑100 (MQ=255)

                                  cccGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATa                                                              >  1:3964242/1‑100 (MQ=255)

                                  cccGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATa                                                              <  2:3259985/100‑1 (MQ=255)

                                    cGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAgg                                                            >  2:1079712/1‑100 (MQ=255)

                                     ggTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGc                                                           <  1:3717166/100‑1 (MQ=255)

                                      gTTTTGTACGCCCAGTCGAAGAATTTCAGCTCTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTGGAGGTAATAGGcc                                                          <  2:2543912/100‑1 (MQ=255)

                                       ttttGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCa                                                         <  2:3510747/100‑1 (MQ=255)

                                         ttGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATg                                                       <  1:1909104/100‑1 (MQ=255)

                                           gTACGCCCAGTCGAATAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCa                                                     <  2:2089355/100‑1 (MQ=255)

                                             aCGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATc                                                   <  2:2193255/100‑1 (MQ=255)

                                             aCGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATc                                                   <  1:2884060/100‑1 (MQ=255)

                                              cGCCCAGTCGAAGAATTTCAGCGCTTCTGTGTCTTGTTCTGGTTTCTACTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCt                                                  <  2:2786684/100‑1 (MQ=255)

                                              cGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCt                                                  >  1:1334676/1‑100 (MQ=255)

                                               gaccAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCtt                                                 >  2:1210449/3‑100 (MQ=255)

                                                cccAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTc                                                >  2:3011838/1‑100 (MQ=255)

                                                 ccAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCg                                               <  2:2882276/100‑1 (MQ=255)

                                                  cAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCg                                               >  1:3424548/1‑99 (MQ=255)

                                                   aGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGcc                                             >  1:1783975/1‑100 (MQ=255)

                                                   aGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGcc                                             <  1:3385544/100‑1 (MQ=255)

                                                    gTCGAAGATTTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCt                                            <  2:2815786/100‑1 (MQ=255)

                                                    gTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCt                                            >  2:2182119/1‑100 (MQ=255)

                                                    gTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCt                                            >  1:2451761/1‑100 (MQ=255)

                                                      cGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCttt                                          >  2:373332/1‑100 (MQ=255)

                                                      cGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCttt                                          <  2:83550/100‑1 (MQ=255)

                                                       gaagaaTTTCAGCACTTCTGTGCCTTGTTGTGGTTTCTTCTATTCTTTGTCGATCAGAATGAAGGTGGGAGGGGTAAGAGGCAGTACATCTTCGCAttt                                          >  2:3773856/1‑99 (MQ=255)

                                                       gaagaaTTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTc                                         >  2:626523/1‑100 (MQ=255)

                                                        aagaaTTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGTTAGAGGTAATAGGCCATGCATCTTCGCCTTTCt                                        >  1:3670334/1‑100 (MQ=255)

                                                          gaaTTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCtgg                                      <  2:1414950/100‑1 (MQ=255)

                                                           aaTTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAAAAAAAATAGTAAAGGTAATAGGCCATGCATCTTCGCCTTTCtggt                                     <  2:3227722/100‑1 (MQ=255)

                                                            aTTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCtggtt                                    <  1:920865/100‑1 (MQ=255)

                                                             tttCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCtggttg                                   <  2:607865/100‑1 (MQ=255)

                                                             tttCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCGGTTCTTTGTGGTTCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCtggttg                                   <  2:4007452/100‑1 (MQ=255)

                                                               tCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGTTTGAGGTAATATGCCATGCATCTTCGCCTTTCtggttggt                                 >  2:2016966/1‑100 (MQ=255)

                                                               ncAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCtggttggt                                 >  2:1032532/2‑100 (MQ=255)

                                                                 aGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCa                               <  1:2097153/100‑1 (MQ=255)

                                                                 aGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCa                               <  1:3840428/100‑1 (MQ=255)

                                                                  gCAGTTCTGTTCTTTGTTCTTTTTTTTTCTGTTCTTTGTGGTTCAGATTGAACTTGGTAGAGGTAATGGGTCATGCATCTTCGCCTTTCTGGTTGGTc                                <  2:2456684/98‑1 (MQ=255)

                                                                   cACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGa                             >  1:2606861/1‑100 (MQ=255)

                                                                   cACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGa                             <  1:4044468/100‑1 (MQ=255)

                                                                    aCTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGAt                            >  1:3249926/1‑100 (MQ=255)

                                                                      ttCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATcc                          <  1:2325414/100‑1 (MQ=255)

                                                                      ttCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATcc                          <  2:507669/100‑1 (MQ=255)

                                                                        cTGTGCCTTGTTCTGCTTGCTTCTGTCCTGTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCCGGTTGGTCAGATCCTg                        <  2:4103814/100‑1 (MQ=255)

                                                                          gtgCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAg                      <  1:1674454/100‑1 (MQ=255)

                                                                           tgCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGc                     >  1:775311/1‑100 (MQ=255)

                                                                            gCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAGTGAACGTGGTAGAGGTAATAGGCCATTCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCg                    >  2:216024/1‑100 (MQ=255)

                                                                            gCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCg                    >  1:412303/1‑100 (MQ=255)

                                                                            gCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCg                    >  1:3381430/1‑100 (MQ=255)

                                                                             cgctGTTCTAGTTTCGTCTGTTCTTTGTGGCTCAGACTGAACGTGGTAGATGTAATAGGCCATGCATCTTCGCTTTTCTGGTTGGTCAGATCCTGAGc                     <  2:656650/95‑1 (MQ=255)

                                                                             ccTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGa                   >  1:420472/1‑100 (MQ=255)

                                                                                tGTTCTGGTTTCTTCTGTTCTTTGTGGAACAGAATGAACGTGGTAGTGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGACAGCTCCTGAGCGAAgg                >  1:3304870/1‑100 (MQ=255)

                                                                                   tCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGAAGGttt             >  2:2263335/1‑100 (MQ=255)

                                                                                    cTGGTTTCTTCTGATCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGAAGGtttt            >  1:2807434/1‑100 (MQ=255)

                                                                                       gTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGAAGGTTTTGCt         >  1:1539080/1‑100 (MQ=255)

                                                                                        tttCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGAAGGTTTTGCTc        <  1:1980851/100‑1 (MQ=255)

                                                                                        tttCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGAAGGTTTTGCTc        >  2:1551969/1‑100 (MQ=255)

                                                                                         ttcttcTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGGATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGAAGGTTTTGCTcc       <  1:2037683/100‑1 (MQ=255)

                                                                                         ttcttcTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGAAGGTTTTGCTcc       >  1:2640406/1‑100 (MQ=255)

                                                                                         ttcttcTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGAAGGTTTTGCTcc       <  2:420469/100‑1 (MQ=255)

                                                                                         ttcttcTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGAAGGTTTTGCTcc       <  1:1115979/100‑1 (MQ=255)

                                                                                           cttctGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGAAGGTTTTGCTCCAg     >  2:3450354/1‑100 (MQ=255)

                                                                                           cttctGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGAAGGTTTTGCTCCAg     <  2:2570826/100‑1 (MQ=255)

                                                                                             tctGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGAAGGTTTTGCTCCAGTc   <  1:1146048/100‑1 (MQ=255)

                                                                                              ctGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATACTGAGCGAAGGTTTTGCTCCAGTCt  <  2:2755384/100‑1 (MQ=255)

GGCTGGCGTAATCCAGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGATCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGAAGGTTTTGCTCCAGTCT  >  NC_000913/3910565‑3910758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A