Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F212 I0 R1 246 122.0 6097616 93.1% 5676880 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,165,015 T→A 100% D320V (GAT→GTT)  parC ← DNA topoisomerase IV, subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,165,0150TA98.7% 395.4 / ‑4.0 128D320V (GAT→GTT) parCDNA topoisomerase IV, subunit A
Reads supporting (aligned to +/- strand):  ref base T (1/3);  new base A (69/54);  total (71/57)
Fisher's exact test for biased strand distribution p-value = 3.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 128 total aligned reads displayed.

ACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGAT  >  NC_000913/3164919‑3165110
                                                                                                |                                                                                               
acCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTATCCAGAACg                                                                                               >  2:1985099/1‑99 (MQ=255)
   aGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACggtg                                                                                            >  2:2857368/1‑99 (MQ=255)
    gCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACggtggt                                                                                          >  1:2735536/1‑100 (MQ=255)
     ccATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTa                                                                                         >  1:457678/1‑100 (MQ=255)
      cATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAg                                                                                        >  1:835155/1‑100 (MQ=255)
      cATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAg                                                                                        >  1:625770/1‑100 (MQ=255)
      cATTCGGAGAGGATTTCCAGCAGGTTTTGCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAg                                                                                        <  1:1178348/100‑1 (MQ=255)
       aTTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAAGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGc                                                                                       >  1:43706/1‑100 (MQ=255)
          cGGAGAGGATTTCCAGCAGGTTTTTCACCGCGGGACGACCATGCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGaa                                                                                    <  1:222600/100‑1 (MQ=255)
           ggAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGGCCATCAAAACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAggcaag                                                                                   >  2:332195/1‑95 (MQ=255)
           ggAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAag                                                                                   <  2:933820/100‑1 (MQ=255)
            gagaGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAaga                                                                                  <  2:1885973/100‑1 (MQ=255)
            gagaGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAaga                                                                                  <  1:23345/100‑1 (MQ=255)
              gagGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGGGGTAGCAAagacg                                                                                >  2:2774242/1‑98 (MQ=255)
                ggATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAggtg                                                                              >  2:1529920/1‑100 (MQ=255)
                ggATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGAACGATCATACTAAGGTTAATACGATAGCTTTTTTCCAGAACGGTGGTAGCGAAGAggtg                                                                              >  2:10905/1‑100 (MQ=255)
                ggATTTCCAGAAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTCCCAGAACGGAAGAAGCGaaaa                                                                                  >  2:2354762/1‑94 (MQ=255)
                 gATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATATGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAggtgg                                                                             <  2:1876797/100‑1 (MQ=255)
                  aTTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAggtggt                                                                            <  1:2813725/100‑1 (MQ=255)
                  aTTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAggtg                                                                              >  1:1615707/1‑98 (MQ=255)
                  aTTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAACACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAggtggt                                                                            <  2:1019347/100‑1 (MQ=255)
                   tttCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGtt                                                                           >  1:2613066/1‑100 (MQ=255)
                   tttCCAGCAGGTTTTTCACCACAGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACTGTGGTAGCGAAGAggtgaat                                                                           >  2:1144297/1‑97 (MQ=255)
                    ttCCAGCAGGTTTTTCTCCGCCGGACTTCCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTtc                                                                          <  1:311917/100‑1 (MQ=255)
                     tCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGCACGGTGGTAGCGAAGCGGTGGTtca                                                                         >  2:1204246/1‑100 (MQ=255)
                     tCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTtca                                                                         <  2:2762453/100‑1 (MQ=255)
                     tCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTtca                                                                         <  1:1114072/100‑1 (MQ=255)
                     tCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGGTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAaga                                                                                  >  2:792120/1‑91 (MQ=255)
                      ccAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTtcat                                                                        >  1:1283921/1‑100 (MQ=255)
                      ccAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTtca                                                                         <  2:2156807/99‑1 (MQ=255)
                       cagcagGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTtcatc                                                                       >  1:1165769/1‑100 (MQ=255)
                         gcagGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCAc                                                                     <  2:1008409/100‑1 (MQ=255)
                          cagGTTTTTCACCGCCGGACGACCATCCAGACCGATCTTATTAAGGTTAATACGATAGCTCTTTTCCAGAACTTTGGTAGCGAAGAGGTGGTTCATCAcc                                                                    <  2:1951994/100‑1 (MQ=255)
                          cagGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCAcc                                                                    <  1:1902394/100‑1 (MQ=255)
                           agGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCt                                                                   <  2:1793579/100‑1 (MQ=255)
                            ggTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTg                                                                  <  1:2576088/100‑1 (MQ=255)
                             gTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGa                                                                 <  1:1613163/100‑1 (MQ=255)
                             gTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGa                                                                 >  2:432162/1‑100 (MQ=255)
                              tttttCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGAt                                                                >  2:1265678/1‑100 (MQ=255)
                               tgttCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATc                                                               <  2:1837181/98‑1 (MQ=255)
                                tttCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATcc                                                              <  2:2434464/100‑1 (MQ=255)
                                tttCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATcc                                                              <  1:1761043/100‑1 (MQ=255)
                                 ttCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCa                                                             <  1:2996082/100‑1 (MQ=255)
                                   cACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCata                                                           <  2:632002/100‑1 (MQ=255)
                                   cACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCata                                                           >  1:1714279/1‑100 (MQ=255)
                                   cACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGAAAGCCCTTTTCCAGAACGGTCGTAGCCAAGAGGTGGTTCATCACCTCACCCata                                                           >  2:2153638/1‑100 (MQ=255)
                                    gccgccgGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCatat                                                          >  2:2208938/2‑100 (MQ=255)
                                    aCCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCata                                                           <  2:1967965/99‑1 (MQ=255)
                                     ccgccgGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATc                                                         >  1:2711388/1‑100 (MQ=255)
                                     ccgccgGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATc                                                         >  2:375201/1‑100 (MQ=255)
                                      cgccgGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGATGTGGTTCATCACCTGATCCATATc                                                         <  2:2948600/99‑1 (MQ=255)
                                      cgccgGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATcc                                                        >  1:873412/1‑100 (MQ=255)
                                       gccgGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCa                                                       >  1:364372/1‑100 (MQ=255)
                                         cgGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCAcg                                                     >  1:813184/1‑100 (MQ=255)
                                          ggACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCAcgc                                                    >  2:2354194/1‑100 (MQ=255)
                                          ggACGACCATCCAGACCGATCATATTAAGGTTAATAAGATAGCTCTTTTTCAGAACGGTGGTAGCGAAGAAGTGGTTCATCACCTGATCCATATCCAcg                                                     >  2:3036474/1‑99 (MQ=255)
                                               acCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTg                                               <  2:2454176/100‑1 (MQ=255)
                                               acCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTg                                               <  1:432165/100‑1 (MQ=255)
                                               acCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTg                                               <  2:3012661/100‑1 (MQ=255)
                                                 cgtCCAGACCGTTAATATTAAGGTTAATACGTGAGCTCTTTTCCCGGTCGGTAGTAGCGAAGACGTGGTTCATCGCTTTATTCATATCCACGCGGTTgg                                              <  2:3017658/97‑1 (MQ=255)
                                                 cATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGa                                             >  2:2706239/1‑100 (MQ=255)
                                                 cATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGa                                             >  1:2815606/1‑100 (MQ=255)
                                                 cATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGa                                             >  1:2761367/1‑100 (MQ=255)
                                                   tCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAAc                                           >  2:933070/1‑100 (MQ=255)
                                                     cAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCAAAGAAGTAGTTCATCACCTGATCCATATCCACGCGGTTGGAAcgc                                         >  1:899242/1‑100 (MQ=255)
                                                       gACCGATCATATTAAGGTTAATACGCTAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCgg                                       <  2:2649560/100‑1 (MQ=255)
                                                       gACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCgg                                       <  1:995878/100‑1 (MQ=255)
                                                       gACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCgg                                       <  2:2690956/100‑1 (MQ=255)
                                                        aCCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGc                                      <  1:1204257/100‑1 (MQ=255)
                                                         ccGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGca                                     <  1:1490376/100‑1 (MQ=255)
                                                         ccGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGca                                     <  2:1314630/100‑1 (MQ=255)
                                                           gATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGcaca                                   <  2:1186706/100‑1 (MQ=255)
                                                            aTCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACaa                                  >  1:2793018/1‑100 (MQ=255)
                                                            aTCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACaa                                  >  1:1751160/1‑100 (MQ=255)
                                                              cATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCCCAATc                                >  2:1739758/1‑100 (MQ=255)
                                                              cATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATc                                >  1:888372/1‑100 (MQ=255)
                                                               atatTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAGGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCa                               >  2:721542/1‑100 (MQ=255)
                                                                tatTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCAc                              >  2:2526711/1‑100 (MQ=255)
                                                                    aaGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACACGGTTGGAACGCGGCACAATCACCAgg                          >  1:76419/1‑100 (MQ=255)
                                                                      ggTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcg                        >  1:2407522/1‑100 (MQ=255)
                                                                      ggTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcg                        >  2:1322181/1‑100 (MQ=255)
                                                                       gTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcgg                       >  2:2024483/1‑100 (MQ=255)
                                                                        ttAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCAGTTGGAACGCGGCACAATCACCAGGcggg                      >  1:698568/1‑100 (MQ=255)
                                                                             aCGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcgggtcggg                 >  1:1868540/1‑100 (MQ=255)
                                                                              cGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcgggtcgggt                >  2:1509775/1‑100 (MQ=255)
                                                                               gATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGtt               >  2:945079/1‑100 (MQ=255)
                                                                                aTAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTtc              <  1:1881146/100‑1 (MQ=255)
                                                                                 tAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCTCCAGGCGGGTCGGGTtct             <  2:666217/100‑1 (MQ=255)
                                                                                 tAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTtct             >  1:1337872/1‑100 (MQ=255)
                                                                                   gCTCTTTTCCAGAACGGTGGTCGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGCCACAATCAACACGCGAGTCGGGTtcac            >  1:1974908/1‑97 (MQ=255)
                                                                                   gCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCg           <  2:2692422/100‑1 (MQ=255)
                                                                                     tctTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTg         <  1:2813429/100‑1 (MQ=255)
                                                                                      ctTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTgg        >  2:2219631/1‑100 (MQ=255)
                                                                                       ttttCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGt       <  1:2551437/100‑1 (MQ=255)
                                                                                        tttCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTc      <  1:2741231/100‑1 (MQ=255)
                                                                                         ttCCAGAACGGTGGTAGCGAAGAGGAGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCCGGCGGGTCGGGTTCTTGTGGTCa     >  2:2542112/1‑100 (MQ=255)
                                                                                          tCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAg    <  1:1322193/100‑1 (MQ=255)
                                                                                           ccAGAACGGTGGTAGCGAAGAGGTGGTTCCTCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATAACCAGGCGGGACGGATTCTAGTGGTCAGa   >  1:1765854/1‑100 (MQ=255)
                                                                                           ccAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGa   >  1:1599695/1‑100 (MQ=255)
                                                                                            cAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGAt  >  2:1358094/1‑100 (MQ=255)
                                                                                                |                                                                                               
ACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGAT  >  NC_000913/3164919‑3165110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A