Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F56 I0 R1 215 141.2 6785521 95.3% 6466601 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,165,015 T→A 61.4% D320V (GAT→GTT)  parC ← DNA topoisomerase IV, subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,165,0150TA61.4% 82.4 / 143.2 126D320V (GAT→GTT) parCDNA topoisomerase IV, subunit A
Reads supporting (aligned to +/- strand):  ref base T (20/28);  new base A (52/25);  total (72/54)
Fisher's exact test for biased strand distribution p-value = 5.44e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.94e-02

Only 100 of 126 total aligned reads displayed.

CACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGATT  >  NC_000913/3164918‑3165111
                                                                                                 |                                                                                                
  ccAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCggt                                                                                              >  1:1724905/1‑100 (MQ=255)
    aGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACggtgg                                                                                            <  1:1012282/100‑1 (MQ=255)
     gCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCggtggt                                                                                           <  1:3313073/100‑1 (MQ=255)
      ccATTCGGAGAGGCTTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACggtggt                                                                                           >  1:2554935/1‑99 (MQ=255)
       cATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAg                                                                                         >  1:229858/1‑100 (MQ=255)
       cATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAg                                                                                         >  2:1498158/1‑100 (MQ=255)
        aTTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGc                                                                                        <  2:622182/100‑1 (MQ=255)
        aTTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCAAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGc                                                                                        >  2:815010/1‑100 (MQ=255)
          tCGGAGAGGATTTCCAGCAGGTTTTTTTTTTTTTTTTTACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTCGTAGCg                                                                                       >  1:2658145/1‑99 (MQ=255)
           cGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGAGGTAGCGaa                                                                                     >  1:2987264/1‑100 (MQ=255)
            ggAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTCAATACGATCCCCCTTTTCCCGAACGGTGGCAGCGa                                                                                      >  1:2285874/1‑98 (MQ=255)
              agagGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAGTACGATAGCTCTTTTCCAGATCGGTGGTAGCGAagag                                                                                  <  1:1810495/100‑1 (MQ=255)
               gagGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAgg                                                                                 >  1:3309028/1‑100 (MQ=255)
               gagGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAgg                                                                                 <  1:515951/100‑1 (MQ=255)
                agGATTTCNAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAgg                                                                                 >  2:3200044/1‑99 (MQ=255)
                agGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAggt                                                                                <  1:2088549/100‑1 (MQ=255)
                 ggATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACTATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAggtg                                                                               <  2:2395142/100‑1 (MQ=255)
                 ggATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGAGGTAGCGAaga                                                                                   >  1:90954/1‑96 (MQ=255)
                  gATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAggtgg                                                                              <  2:830327/100‑1 (MQ=255)
                  gATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAggtgg                                                                              <  1:1724155/100‑1 (MQ=255)
                   aTTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAggtggt                                                                             <  1:3144763/100‑1 (MQ=255)
                   aTTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAggtggt                                                                             >  2:394898/1‑100 (MQ=255)
                     ttCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTtc                                                                           <  1:723696/100‑1 (MQ=255)
                      tCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTtca                                                                          >  2:1759589/1‑100 (MQ=255)
                      tCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTtca                                                                          <  1:2061229/100‑1 (MQ=255)
                        cagcagGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTtcatc                                                                        >  2:3322396/1‑100 (MQ=255)
                         agcagGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGGTAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTtcatca                                                                       >  1:2795418/1‑100 (MQ=255)
                          gcagGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCAc                                                                      <  1:34361/100‑1 (MQ=255)
                           cagGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCAcc                                                                     >  1:572973/1‑100 (MQ=255)
                           cagGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCAcc                                                                     <  1:1138158/100‑1 (MQ=255)
                           cagGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCAcc                                                                     <  1:511675/100‑1 (MQ=255)
                           cagGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCAcc                                                                     <  2:1509637/100‑1 (MQ=255)
                           cagGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCAcc                                                                     <  1:3351421/100‑1 (MQ=255)
                            agGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCt                                                                    <  1:2551021/100‑1 (MQ=255)
                             ggTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTg                                                                   >  2:1686203/1‑100 (MQ=255)
                             ggTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGACAGCTCTCTTCCAGAACGGTGGTAGCGAAGAGGTCGTTCATCACCTg                                                                   >  2:1668401/1‑100 (MQ=255)
                             ggTTTTTCACCGACGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGATCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTg                                                                   >  2:1668261/1‑100 (MQ=255)
                               tttttCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGAt                                                                 >  2:1583695/1‑100 (MQ=255)
                                ttttCACCGCCGGACGACCATCCAGACCGCTCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTTGTAGCGAAGAGGTGGTTCATCCCCTGATc                                                                >  1:195732/1‑100 (MQ=255)
                                 tttCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATcc                                                               <  2:812909/100‑1 (MQ=255)
                                  ttCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCa                                                              <  1:575435/100‑1 (MQ=255)
                                    cACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCata                                                            >  2:105063/1‑100 (MQ=255)
                                    cACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCata                                                            >  2:1793190/1‑100 (MQ=255)
                                    cACCGCCGGACGACCATCCAGACCGATCATATTAAGGGTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCat                                                             >  1:1036245/1‑99 (MQ=255)
                                    cACCGCCGGACGACCATCCAGACCGATCATATTAAGGATAATACGAGAGCTCTTTTCCAGAACTGTGGTAGCGACGAGGTGGTTAATCACCTTATCCa                                                              >  1:1799417/1‑98 (MQ=255)
                                     aCCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCatat                                                           <  2:1756510/100‑1 (MQ=255)
                                      ccgccgGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATc                                                          >  2:3317180/1‑100 (MQ=255)
                                      ccgccgGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCAAAGAGGTGGTTCATCACCTGATCCATATc                                                          >  1:786197/1‑100 (MQ=255)
                                            gacgacCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCAcgcg                                                    <  1:1427377/100‑1 (MQ=255)
                                              cgacCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGt                                                  <  1:1312760/100‑1 (MQ=255)
                                              cgacCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGt                                                  >  1:1980419/1‑100 (MQ=255)
                                              cgacCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGt                                                  >  1:3376343/1‑100 (MQ=255)
                                              cgacCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGt                                                  >  2:3134016/1‑100 (MQ=255)
                                              cgacCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGt                                                  >  2:1203445/1‑100 (MQ=255)
                                                acCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTg                                                <  1:1272497/100‑1 (MQ=255)
                                                acCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTg                                                <  1:2125971/100‑1 (MQ=255)
                                                 ccATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTgg                                               >  1:560275/1‑100 (MQ=255)
                                                  cATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGa                                              >  1:870773/1‑100 (MQ=255)
                                                    tCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAAc                                            >  1:1307710/1‑100 (MQ=255)
                                                     ccAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAAcg                                           <  2:1519719/100‑1 (MQ=255)
                                                      cAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAAcgc                                          >  2:103159/1‑100 (MQ=255)
                                                        gACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCgg                                        <  2:2258679/100‑1 (MQ=255)
                                                        gACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCgg                                        >  1:189914/1‑100 (MQ=255)
                                                        gACCGAAGAGATGAAGGTTAATACGATAGCTCTTTTCCAGAGCGCTGGTAGCGAAGAGGTGGTGCATCACCTGATCCATATCCACGCGGTTGGAACGCgg                                        <  1:1815658/100‑1 (MQ=255)
                                                           cGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGcac                                     >  1:379316/1‑100 (MQ=255)
                                                           cGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGcac                                     <  1:1924296/100‑1 (MQ=255)
                                                              tCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAAt                                  >  1:1591329/1‑100 (MQ=255)
                                                                atatTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGACGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCa                                >  2:1998590/1‑100 (MQ=255)
                                                                 tatTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCAc                               <  2:1185835/100‑1 (MQ=255)
                                                                  atTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCAcc                              <  2:3010917/100‑1 (MQ=255)
                                                                   ttAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCa                             <  2:2353873/100‑1 (MQ=255)
                                                                     aaGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAgg                           <  2:3156487/100‑1 (MQ=255)
                                                                     aaGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAgg                           >  2:2447338/1‑100 (MQ=255)
                                                                      aGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGc                          >  2:1191654/1‑100 (MQ=255)
                                                                       ggTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcg                         >  2:279311/1‑100 (MQ=255)
                                                                        gTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcgg                        >  2:1949611/1‑100 (MQ=255)
                                                                         ttAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcggg                       <  1:1770288/100‑1 (MQ=255)
                                                                          tAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcgggt                      >  1:2544294/1‑100 (MQ=255)
                                                                            aTACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcgggtcg                    >  1:3142063/1‑100 (MQ=255)
                                                                            aTACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcgggtcg                    >  2:401592/1‑100 (MQ=255)
                                                                             tACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcgggtcgg                   >  1:959097/1‑100 (MQ=255)
                                                                             tACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcgggtcgg                   >  1:959120/1‑100 (MQ=255)
                                                                              aCGATAGCTCTTTTCCAGAACGGTGGTAGTGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcgggtcggg                  <  1:697565/100‑1 (MQ=255)
                                                                               cGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcgggtcgggt                 <  2:2253239/100‑1 (MQ=255)
                                                                               cGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcgggtcgggt                 <  2:3254068/100‑1 (MQ=255)
                                                                               cGACAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcgggtcgggt                 <  1:2453813/100‑1 (MQ=255)
                                                                                gATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGtt                >  1:1259298/1‑100 (MQ=255)
                                                                                gATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGtt                >  1:1740905/1‑100 (MQ=255)
                                                                                     ctctTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACTCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTTTCGt           >  2:1178283/1‑100 (MQ=255)
                                                                                     ctctTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGt           <  1:1527995/100‑1 (MQ=255)
                                                                                     ctctTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGCTCTCGt           >  1:2455303/1‑100 (MQ=255)
                                                                                     ctctTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATACACGCGGTTGGAAAGCGGCACAATCACCAGGCGGGACGGGTTCTCGt           >  1:387554/1‑100 (MQ=255)
                                                                                      tctTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTg          >  2:1460363/1‑100 (MQ=255)
                                                                                        ttttCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTgg         >  1:1676832/1‑99 (MQ=255)
                                                                                         tttCCAGATCGGTGGTAGCGAAGAGGTGGGTCATCACCTGATCCAGATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTc       <  1:550430/100‑1 (MQ=255)
                                                                                          ttCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCa      >  2:2613807/1‑100 (MQ=255)
                                                                                          ttCCAGATCGGTGGTAGCGAAGAGGTGGGTCAGCACCTGATCCATGTCCAGGCGGTTGGAACGTGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCa      <  1:1162751/100‑1 (MQ=255)
                                                                                            ccAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGCTTCTCGTGGTCAGa    >  1:405754/1‑100 (MQ=255)
                                                                                             cAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGAt   <  2:512915/100‑1 (MQ=255)
                                                                                             cAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGAt   >  1:156111/1‑100 (MQ=255)
                                                                                                 |                                                                                                
CACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGATT  >  NC_000913/3164918‑3165111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A