Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F256 I0 R1 328 102.7 5123138 93.4% 4785010 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,165,015 T→A 100% D320V (GAT→GTT)  parC ← DNA topoisomerase IV, subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,165,0150TA99.4% 305.2 / ‑6.3 98D320V (GAT→GTT) parCDNA topoisomerase IV, subunit A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (59/38);  minor base C (1/0);  total (60/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

ACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGAT  >  NC_000913/3164919‑3165110
                                                                                                |                                                                                               
acCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCTCCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACgg                                                                                              <  1:1799277/100‑1 (MQ=255)
acCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACgg                                                                                              <  1:2165808/100‑1 (MQ=255)
  cAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACGATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACggtg                                                                                            >  2:2342146/1‑100 (MQ=255)
  cAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACggtg                                                                                            >  1:1391619/1‑100 (MQ=255)
  cAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACggtg                                                                                            <  1:1505911/100‑1 (MQ=255)
   aGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACggtgg                                                                                           <  2:757175/100‑1 (MQ=255)
    gCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACggtggt                                                                                          <  1:680959/100‑1 (MQ=255)
    gCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACggtggt                                                                                          <  2:49139/100‑1 (MQ=255)
    gCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACggtggt                                                                                          <  1:787831/100‑1 (MQ=255)
      cATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAg                                                                                        >  2:2070569/1‑100 (MQ=255)
       aTTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGc                                                                                       <  2:1486605/100‑1 (MQ=255)
       aTTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGc                                                                                       <  2:801933/100‑1 (MQ=255)
        ttCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCg                                                                                      <  2:964436/100‑1 (MQ=255)
         tCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGa                                                                                     <  2:2450499/100‑1 (MQ=255)
           ggAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAag                                                                                   >  1:1657613/1‑100 (MQ=255)
           ggAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAag                                                                                   <  1:821334/100‑1 (MQ=255)
            gagaGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAaga                                                                                  >  1:1425810/1‑100 (MQ=255)
              gagGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAgg                                                                                <  1:58934/100‑1 (MQ=255)
               agGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAggt                                                                               <  1:761630/100‑1 (MQ=255)
                ggATTTCCAGCAGGTTTTTCACCGCTGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGCGGCAGc                                                                                       >  2:861947/1‑91 (MQ=255)
                ggATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAggtg                                                                              >  1:1221199/1‑100 (MQ=255)
                ggATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAggtg                                                                              >  1:2264820/1‑100 (MQ=255)
                ggATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATAATATTACGGTTAATACGATAGCTCTTTTCCAGACCGGTGCTAGCAAAGAggt                                                                               >  1:2138435/1‑99 (MQ=255)
                  aTTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAggtggt                                                                            >  1:331859/1‑100 (MQ=255)
                  aTTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAggtggt                                                                            <  2:1430010/100‑1 (MQ=255)
                  aTTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAggtggt                                                                            >  1:2023315/1‑100 (MQ=255)
                   tttCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGtt                                                                           >  2:1945337/1‑100 (MQ=255)
                     tCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATAGTAAGGTTAATACGCTAGCTCCTTTCCAGAACGGTGGTAACGAagag                                                                                 >  2:2051195/1‑92 (MQ=255)
                      ccAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTtcat                                                                        >  1:1248975/1‑100 (MQ=255)
                        agcagGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTtcatca                                                                      >  1:33653/1‑100 (MQ=255)
                        agcagGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTtcatc                                                                       >  1:2134843/1‑99 (MQ=255)
                        agcagGTTTTTCACCGCAGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCAAGAACGGTGGTAGCGAAGAGGTGGTtcataa                                                                      >  2:1650231/1‑98 (MQ=255)
                         gcagGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCAc                                                                     >  2:1164172/1‑100 (MQ=255)
                         gcagGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCAc                                                                     >  1:272048/1‑100 (MQ=255)
                          cagGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCAcc                                                                    <  1:1219220/100‑1 (MQ=255)
                            ggTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTg                                                                  >  1:72315/1‑100 (MQ=255)
                            ggTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTg                                                                  >  2:576336/1‑100 (MQ=255)
                            ggTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTg                                                                  >  2:871359/1‑100 (MQ=255)
                             gTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCACAACGTTGGTACCGAAGAGGTGGGTCATCACCTGa                                                                 >  2:2313727/1‑100 (MQ=255)
                              tttttCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGAt                                                                >  1:2549253/1‑100 (MQ=255)
                              tttttCACCGCCGGACGACCATCCAGACCGATCATATTAAGGGTAATACGATAGCTCTTTTCCAGAACGCTGGTCGCGCAGAGGTGGTTCCTCAACtca                                                                 >  2:1323217/1‑97 (MQ=255)
                               ttttCACCTCCTGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATc                                                               >  2:2156077/1‑100 (MQ=255)
                                 ttCACCGCCGGACGACCATCCATACCGATCATATTAAGGCTCATACGATAGCTCTTTCCCAGAACGGAGGTAGCGAACAGGTGGTACATCCCCCGa                                                                 >  2:2215656/1‑96 (MQ=255)
                                   caccggcaaacgacCACCCAGACCCATCATATTAAGGTTAATACGAGAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATcc                                                              <  2:1883243/88‑1 (MQ=255)
                                    aCCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTGTTTTCCAGAACGGTGGTAGCGCAGAGGTGGTTCATCACCTGAACCatat                                                          >  2:1691199/1‑100 (MQ=255)
                                     ccgccgGACGAACATCCAGACCGATCATATTAAGGTTAAGACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGCGGTGGTGCATCGCCTGATCCATATc                                                         >  2:473418/1‑100 (MQ=255)
                                      cgccgGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATcc                                                        >  1:8876/1‑100 (MQ=255)
                                      cgccgGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATcc                                                        <  1:696176/100‑1 (MQ=255)
                                        ccgGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCAc                                                      <  2:2550633/100‑1 (MQ=255)
                                        ccgGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCAc                                                      >  1:1869643/1‑100 (MQ=255)
                                          ggACGACCATCCAGACCGATCATATTAAGGTTAAGACGAGAGCTCTTGTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCAcgc                                                    <  2:2146390/100‑1 (MQ=255)
                                             cgacCATCCAGACCGATCATATTAAGGTTAACACGATAGCTCTTTTCCAGAACGGTGGTAGCGAACAGCACGTCCATCACCTGATCCATATCCCCGCgg                                                  >  2:1595362/1‑99 (MQ=255)
                                               acCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTg                                               >  1:613752/1‑100 (MQ=255)
                                                ccATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTgg                                              >  2:2111270/1‑100 (MQ=255)
                                                 cATCCAGACCGATCATATTAAGGTTAATACGATAGCTTTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGa                                             >  2:1363849/1‑100 (MQ=255)
                                                 cATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGa                                             >  1:1663057/1‑100 (MQ=255)
                                                 cATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGa                                             >  2:2004936/1‑100 (MQ=255)
                                                  aTCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGaa                                            >  1:1992679/1‑100 (MQ=255)
                                                   tCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAAc                                           <  1:81746/100‑1 (MQ=255)
                                                     cAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAAcgc                                         >  2:8619/1‑100 (MQ=255)
                                                     cAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAAcg                                          >  2:1471284/1‑99 (MQ=255)
                                                       gtccGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCgg                                       <  2:1463808/98‑1 (MQ=255)
                                                        aCCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGc                                      <  2:2084612/100‑1 (MQ=255)
                                                         ccGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGca                                     <  2:1854860/100‑1 (MQ=255)
                                                         ccGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGca                                     <  1:2315809/100‑1 (MQ=255)
                                                           gATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGcaca                                   >  2:415924/1‑100 (MQ=255)
                                                              cATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCATCTGATCCATATCCACGCGGTTGGAACGCGGCACAATc                                <  2:1376282/100‑1 (MQ=255)
                                                              cATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATc                                <  2:270101/100‑1 (MQ=255)
                                                               atatTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCa                               <  2:591588/100‑1 (MQ=255)
                                                                tatTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCAc                              >  1:935018/1‑100 (MQ=255)
                                                                tatTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCAc                              >  2:1195639/1‑100 (MQ=255)
                                                                tatTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCAc                              >  2:1861656/1‑100 (MQ=255)
                                                                tatTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATAGCCACGCGGTTGGAACGCGGCACAATCAc                              >  2:2043322/1‑100 (MQ=255)
                                                                  ttAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCa                            <  1:1307450/100‑1 (MQ=255)
                                                                   tAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAg                           >  2:2499669/1‑100 (MQ=255)
                                                                   tAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACAAg                           >  2:580975/1‑100 (MQ=255)
                                                                      ggTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcg                        >  1:2271610/1‑100 (MQ=255)
                                                                      ggTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcg                        >  1:738879/1‑100 (MQ=255)
                                                                      ggTTAATACGATAGCTCTTGTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcg                        <  2:2256769/100‑1 (MQ=255)
                                                                       gTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcgg                       >  2:1872592/1‑100 (MQ=255)
                                                                       gTTAATACGATAGCTCTTTTACAGAACGGTGGTAGCGAAGAGGTGGTTCATCACTTGATCCATATACACGCGGTTGGAACGCGGGACAATCACCAGGcgg                       >  2:1124018/1‑100 (MQ=255)
                                                                        ttAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcggg                      <  2:879515/100‑1 (MQ=255)
                                                                           aTACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcgggtcg                   >  2:1302470/1‑100 (MQ=255)
                                                                             aCGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcgggtcggg                 <  1:1861670/100‑1 (MQ=255)
                                                                             aCGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcgggccggg                 >  2:395480/1‑100 (MQ=255)
                                                                              cGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcgggtcgggt                <  1:335378/100‑1 (MQ=255)
                                                                               gATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcgggccgggt                >  1:763558/1‑99 (MQ=255)
                                                                                   gCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCg           <  1:871367/100‑1 (MQ=255)
                                                                                   gCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCg           >  1:891283/1‑100 (MQ=255)
                                                                                    ctctTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGt          >  1:1630329/1‑100 (MQ=255)
                                                                                     tctTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTg         <  2:2409896/100‑1 (MQ=255)
                                                                                      ctTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTgg        >  1:1425034/1‑100 (MQ=255)
                                                                                      ctTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTgg        <  2:1571020/100‑1 (MQ=255)
                                                                                       ttttCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGt       <  1:899585/100‑1 (MQ=255)
                                                                                          tCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAg    >  2:973158/1‑100 (MQ=255)
                                                                                           ccAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGa   <  1:1447032/100‑1 (MQ=255)
                                                                                            cAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCGCAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGAt  >  2:808483/1‑100 (MQ=255)
                                                                                            cAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGAt  >  2:271065/1‑100 (MQ=255)
                                                                                                |                                                                                               
ACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGAT  >  NC_000913/3164919‑3165110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A