Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F204 I0 R1 247 174.3 8556966 94.2% 8060661 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,720,629 T→C 38.1% T66A (ACT→GCT)  slyA ← global transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,720,6290TC38.1% 81.5 / 123.8 118T66A (ACT→GCT) slyAglobal transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (34/39);  new base C (21/24);  total (55/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.23e-01

Only 100 of 119 total aligned reads displayed.

GGCTCTGCCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTGTAACGTAACCCAATG  >  NC_000913/1720532‑1720728
                                                                                                 |                                                                                                   
ggCTCTGCCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTAc                                                                                                   >  2:3150805/1‑100 (MQ=255)
ggCTCTGCCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCAc                                                                                                   <  1:1154198/100‑1 (MQ=255)
ggCTCTGCCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCAc                                                                                                   <  1:295468/100‑1 (MQ=255)
 gCTCTGCCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAGCCCTTTTTCTTCCAGTTGGTCCAGAGCACg                                                                                                  >  1:1147228/1‑100 (MQ=255)
  ctctGCCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACgg                                                                                                 >  1:3117688/1‑100 (MQ=255)
   tctGCCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGa                                                                                                <  1:1730569/100‑1 (MQ=255)
     tGCCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGAcc                                                                                              >  2:2566005/1‑100 (MQ=255)
     tGCCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGAcn                                                                                              <  1:3916727/100‑2 (MQ=255)
      gCCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCa                                                                                             >  1:103141/1‑100 (MQ=255)
       ccTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGCGTACGGACCAg                                                                                            >  2:3562963/1‑100 (MQ=255)
       ccTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAg                                                                                            >  2:1318605/1‑100 (MQ=255)
        cTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGt                                                                                           >  2:2967072/1‑100 (MQ=255)
            tCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAGCCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATg                                                                                       <  2:1147231/100‑1 (MQ=255)
            tCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATg                                                                                       >  1:106429/1‑100 (MQ=255)
            tCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATg                                                                                       <  2:1452551/100‑1 (MQ=255)
             ccGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAGATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATgg                                                                                      <  1:2895620/100‑1 (MQ=255)
              cGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGgc                                                                                     <  1:28444/100‑1 (MQ=255)
              cGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGgc                                                                                     >  2:2867826/1‑100 (MQ=255)
              cGTCAGTTTAATAAGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTAGGTCCAGAGTACGGACCAGTGATGgc                                                                                     <  1:628771/100‑1 (MQ=255)
               gTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGgct                                                                                    >  2:185499/1‑100 (MQ=255)
                 cAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGgctgc                                                                                  <  2:6360/100‑1 (MQ=255)
                   gTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTc                                                                                >  2:373148/1‑100 (MQ=255)
                   gTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTc                                                                                >  2:2116910/1‑100 (MQ=255)
                    tttAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCg                                                                               <  1:1437348/100‑1 (MQ=255)
                    tttAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCg                                                                               <  2:4264004/100‑1 (MQ=255)
                     ttAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGa                                                                              >  2:3067004/1‑100 (MQ=255)
                     ttAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTATTCCAGTTGGTCCAGAGTACGGACCAGTGATGGATGCTCGa                                                                              >  2:3564934/1‑100 (MQ=255)
                      tAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGAt                                                                             <  2:361889/100‑1 (MQ=255)
                       aaTACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATg                                                                            <  1:588362/100‑1 (MQ=255)
                        aTACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGc                                                                           >  2:3867363/1‑100 (MQ=255)
                        aTACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGc                                                                           <  1:332185/100‑1 (MQ=255)
                         tACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGcc                                                                          <  1:3639030/100‑1 (MQ=255)
                          aCGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCa                                                                         <  2:1222467/100‑1 (MQ=255)
                            gTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAAt                                                                       <  2:889351/100‑1 (MQ=255)
                            gTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAAt                                                                       <  1:1877221/100‑1 (MQ=255)
                              ttAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCg                                                                     >  2:1299504/1‑100 (MQ=255)
                              ttAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCg                                                                     <  2:2528279/100‑1 (MQ=255)
                                aGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCt                                                                   >  2:3138585/1‑100 (MQ=255)
                                 gCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCtt                                                                  >  2:1157548/1‑100 (MQ=255)
                                 gCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCtt                                                                  >  1:3268266/1‑100 (MQ=255)
                                 gCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCtt                                                                  >  2:2311874/1‑100 (MQ=255)
                                 gCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGCCTGCTCGATGCCAATCGCt                                                                   >  2:1618266/1‑99 (MQ=255)
                                   ccGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCtttt                                                                >  2:464432/1‑100 (MQ=255)
                                   ccGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCtttt                                                                >  1:1910981/1‑100 (MQ=255)
                                     gacgaTCGCTTGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGAGCATTGATGGCTGCTCGATGCCAATCGCTTTTGc                                                              <  1:2810738/100‑1 (MQ=255)
                                      acgaTCGCTGGCACAAGTTTGACGCAAAATTAACCCGTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGcc                                                             <  2:1910985/100‑1 (MQ=255)
                                        gaTCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAg                                                           >  1:1087020/1‑100 (MQ=255)
                                         aTCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTTTTCCAGTTGGTCCAGAGTAAGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGcaa                                                            >  1:3312531/1‑96 (MQ=255)
                                          tCGCTGGCACAAGTTTGACGCTCAATTAACCCTTTAGCGTCCTGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAg                                                           <  1:1656203/98‑1 (MQ=255)
                                           cGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTg                                                        <  2:4241019/100‑1 (MQ=255)
                                               ggCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAAtt                                                    <  2:90005/100‑1 (MQ=255)
                                                 cacaAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTg                                                  <  1:2204189/100‑1 (MQ=255)
                                                 cacaAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAAttt                                                   >  2:162027/1‑99 (MQ=255)
                                                 cacaAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTg                                                  >  2:1639694/1‑100 (MQ=255)
                                                  acaAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGc                                                 <  2:3814544/100‑1 (MQ=255)
                                                  acaAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGc                                                 <  1:1580012/100‑1 (MQ=255)
                                                   caAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTTCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCg                                                <  1:952600/100‑1 (MQ=255)
                                                   caAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCg                                                >  1:2002797/1‑100 (MQ=255)
                                                   caAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCg                                                >  2:2855242/1‑100 (MQ=255)
                                                   caAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCg                                                >  1:1997642/1‑100 (MQ=255)
                                                    aaGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGa                                               >  2:2823117/1‑100 (MQ=255)
                                                     aGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGAc                                              <  2:714287/100‑1 (MQ=255)
                                                     aGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGAc                                              <  2:3522295/100‑1 (MQ=255)
                                                          gACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTc                                         <  2:3753841/100‑1 (MQ=255)
                                                            cgcgAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTg                                       >  2:1935177/1‑100 (MQ=255)
                                                            cgcgAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTg                                       >  1:4142206/1‑100 (MQ=255)
                                                            cgcgAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTg                                       >  1:1577940/1‑100 (MQ=255)
                                                            cgcgAAATTAACCCTTTTTCTTCCAGTTGGGCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTGGCCAGTTGAATTTGGGAGTGGtgtg                                       >  1:982938/1‑100 (MQ=255)
                                                             gcgAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTgg                                      <  2:3115256/100‑1 (MQ=255)
                                                             gcgAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTgg                                      <  2:3638425/100‑1 (MQ=255)
                                                             gcgAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTgg                                      <  2:1980385/100‑1 (MQ=255)
                                                              cgAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGa                                     <  2:3268278/100‑1 (MQ=255)
                                                              cgAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAATTGAATTTGCGACTGGTCTGGa                                     <  2:3847410/100‑1 (MQ=255)
                                                                aaaTTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAgg                                   <  2:1382510/100‑1 (MQ=255)
                                                                  aTTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTa                                 <  1:1254308/100‑1 (MQ=255)
                                                                  aTTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTa                                 <  2:3546522/100‑1 (MQ=255)
                                                                     aaCCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGTCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACt                              <  1:818212/100‑1 (MQ=255)
                                                                     aaCCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACt                              <  1:3867346/100‑1 (MQ=255)
                                                                     aaCCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACt                              <  1:3867027/100‑1 (MQ=255)
                                                                        ccTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTACCTGAt                           >  1:3443997/1‑100 (MQ=255)
                                                                         cTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATg                          >  2:1804468/1‑100 (MQ=255)
                                                                          tttttCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATgg                         <  2:1898715/100‑1 (MQ=255)
                                                                            tttCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGat                       <  2:646814/100‑1 (MQ=255)
                                                                             ttcttcCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGata                      <  2:106429/100‑1 (MQ=255)
                                                                             ttcttcCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTGGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGat                       <  1:1114922/99‑1 (MQ=255)
                                                                                 tcCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATtgt                  >  2:1238050/1‑100 (MQ=255)
                                                                                     gTTGGTCCAGAGTACGGACCAGCGATGGCTGATCGATTCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTgtaa              <  1:2888904/100‑1 (MQ=255)
                                                                                      ttGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTgtaac             <  1:1740284/100‑1 (MQ=255)
                                                                                      ttGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTgtaac             <  2:24196/100‑1 (MQ=255)
                                                                                       tGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTgtaacg            <  2:4086414/100‑1 (MQ=255)
                                                                                         gTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTgtaacgta          >  1:2372264/1‑100 (MQ=255)
                                                                                         gTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTgtaacgta          >  2:1309003/1‑100 (MQ=255)
                                                                                         gTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTATTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTgtaacgta          >  1:90746/1‑100 (MQ=255)
                                                                                          tCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTgtaacgtaa         >  2:3808934/1‑100 (MQ=255)
                                                                                          tCCACAGTACGGCCCAGTGATCGCAGCTCGATGCCTATCGCTTTTGCCAGTTCAATTTCCTACTGGTCTGGAGGTACCTGATCGATATTGTgtaatgtc          <  1:2545536/99‑2 (MQ=255)
                                                                                           ccAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTgtaacgtaac        <  1:1595071/100‑1 (MQ=255)
                                                                                             agagTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTGTAACGTAAccc      >  1:2151624/1‑100 (MQ=255)
                                                                                             agagTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTGTAACGTAAccc      >  2:1310402/1‑100 (MQ=255)
                                                                                             agagCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTGTAACGTAAccc      <  2:2061013/100‑1 (MQ=255)
                                                                                                 tACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTGTAACGTAACCCAATg  <  2:3346263/100‑1 (MQ=255)
                                                                                                 |                                                                                                   
GGCTCTGCCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTGTAACGTAACCCAATG  >  NC_000913/1720532‑1720728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A