Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F133 I0 R1	200	171.4	8351354	94.3%	7875326	99.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,720,629	T→C	74.4%	T66A (ACT→GCT)	slyA ←	global transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,720,629	0	T	C	74.4%	214.6 / 75.3	127	T66A (ACT→GCT)	slyA	global transcriptional regulator
Reads supporting (aligned to +/- strand): ref base T (14/18); new base C (42/52); total (56/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

Only 100 of 127 total aligned reads displayed.

GCTCTGCCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTGTAACGTAACCC  >  NC_000913/1720533‑1720724

gCTCTGCCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTAc                                                                                               <  2:2016855/99‑1 (MQ=255)

gCTCTGCCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACg                                                                                              >  1:1353676/1‑100 (MQ=255)

 ctctGCCTTTTCCTTCAGTTTAATACGTTTAGCCCTACGATCTCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACgg                                                                                             <  2:3707659/100‑1 (MQ=255)

  tctGCCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGa                                                                                            <  2:3393929/100‑1 (MQ=255)

    tGCCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCTAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGAcc                                                                                          <  1:348752/100‑1 (MQ=255)

    tGCCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAAGTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGAcc                                                                                          <  2:3582230/100‑1 (MQ=255)

       cTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTTGTCCAGAGTACGGACCAGt                                                                                       >  2:2970956/1‑100 (MQ=255)

       cTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGt                                                                                       >  1:2176805/1‑100 (MQ=255)

          ttCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGAt                                                                                    <  1:1160120/100‑1 (MQ=255)

            ccGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGCGCACGGACCAGTGATgg                                                                                  >  2:223731/1‑100 (MQ=255)

            ccGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATgg                                                                                  >  1:1741547/1‑100 (MQ=255)

             cGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGgc                                                                                 <  1:2499101/100‑1 (MQ=255)

                cAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGgctgc                                                                              >  1:241482/1‑100 (MQ=255)

                cAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGgctgc                                                                              >  2:2624684/1‑100 (MQ=255)

                 aGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGgctgct                                                                             >  2:1609460/1‑100 (MQ=255)

                  gTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTc                                                                            <  2:647898/100‑1 (MQ=255)

                  gTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTc                                                                            >  1:1236140/1‑100 (MQ=255)

                   tttAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCg                                                                           >  1:2751463/1‑100 (MQ=255)

                     tAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCTAGAGTACGGACCAGTGATGGCTGCTCGAt                                                                         >  2:3229129/1‑100 (MQ=255)

                     tAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGAt                                                                         <  1:1509849/100‑1 (MQ=255)

                      aaTACGTTTAGCGCGCCGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTGTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATg                                                                        <  2:1870897/100‑1 (MQ=255)

                      aaTACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATg                                                                        >  1:541737/1‑100 (MQ=255)

                        tACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGcc                                                                      <  1:1366983/100‑1 (MQ=255)

                        tACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTGCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGcc                                                                      >  2:2659368/1‑100 (MQ=255)

                           gTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAAt                                                                   <  2:1396144/100‑1 (MQ=255)

                            tttAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATc                                                                  <  1:1217279/100‑1 (MQ=255)

                              tAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGc                                                                <  1:638315/100‑1 (MQ=255)

                               aGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCt                                                               <  2:706163/100‑1 (MQ=255)

                                gCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCtt                                                              >  2:4130625/1‑100 (MQ=255)

                                  ccGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCtttt                                                            >  1:2585206/1‑100 (MQ=255)

                                   cgacgaTCGCTGGCACAAGTTTCACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCttt                                                             <  2:3095457/98‑1 (MQ=255)

                                    gacgaTCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGc                                                          <  1:1340349/100‑1 (MQ=255)

                                     acgaTCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGcc                                                         <  2:3729186/100‑1 (MQ=255)

                                     acgaTCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGcc                                                         <  2:3879487/100‑1 (MQ=255)

                                     acgaTCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGcc                                                         <  1:3529363/100‑1 (MQ=255)

                                        aTCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCATTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAg                                                       <  2:477044/99‑1 (MQ=255)

                                          cGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTg                                                    <  2:445054/100‑1 (MQ=255)

                                          cGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTg                                                    >  1:521261/1‑100 (MQ=255)

                                          cGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTg                                                    <  2:1000996/100‑1 (MQ=255)

                                           gCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGtt                                                     >  2:2046653/1‑98 (MQ=255)

                                           gCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTg                                                    <  2:155597/99‑1 (MQ=255)

                                            cTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGaa                                                  <  1:1495345/100‑1 (MQ=255)

                                              ggCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAAtt                                                <  2:4008116/100‑1 (MQ=255)

                                              ggCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAAtt                                                >  1:2468964/1‑100 (MQ=255)

                                                cacaAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTg                                              >  1:3042619/1‑100 (MQ=255)

                                                 acaAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGc                                             <  1:3561975/100‑1 (MQ=255)

                                                 acaAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGc                                             <  1:3699430/100‑1 (MQ=255)

                                                  caAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCg                                            >  1:1084042/1‑100 (MQ=255)

                                                  caAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCg                                            <  1:505207/100‑1 (MQ=255)

                                                  caAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCg                                            <  1:857521/100‑1 (MQ=255)

                                                    aGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGAc                                          <  1:3791083/100‑1 (MQ=255)

                                                    aGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGAc                                          <  1:1731248/100‑1 (MQ=255)

                                                    aGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGAc                                          >  1:2657811/1‑100 (MQ=255)

                                                     gTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACt                                         >  1:1517452/1‑100 (MQ=255)

                                                      tttGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACtn                                        <  1:1345289/100‑2 (MQ=255)

                                                       ttGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTgg                                       <  2:3810480/100‑1 (MQ=255)

                                                        tGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGt                                      >  1:1110281/1‑100 (MQ=255)

                                                         gACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTc                                     <  2:3515719/100‑1 (MQ=255)

                                                           cgcgAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTg                                   >  2:2800876/1‑100 (MQ=255)

                                                           cgcgAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTg                                   >  2:187910/1‑100 (MQ=255)

                                                           cgcgAAATTAACCCTTTCTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTg                                   >  1:1161803/1‑100 (MQ=255)

                                                            gcgAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTgg                                  <  1:1554911/100‑1 (MQ=255)

                                                            gcgAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTgg                                  >  1:3453228/1‑100 (MQ=255)

                                                              gAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAg                                >  2:1960129/1‑100 (MQ=255)

                                                               aaaTTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAgg                               <  1:3818434/100‑1 (MQ=255)

                                                               aaaTTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAgg                               <  1:3493066/100‑1 (MQ=255)

                                                                aaTTAACCCTTTTTCTTCCAGTTGGTCCAGAGGACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAgg                               <  1:322870/99‑1 (MQ=255)

                                                                 aTTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTa                             <  1:672143/100‑1 (MQ=255)

                                                                 aTTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTa                             <  1:671915/100‑1 (MQ=255)

                                                                 aTTAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTa                             <  2:3107516/100‑1 (MQ=255)

                                                                   tAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAAc                           <  1:838790/100‑1 (MQ=255)

                                                                   tAACCCTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAAc                           >  1:2164504/1‑100 (MQ=255)

                                                                   tAACCCTTTTTCGTCCAGTTGGTCCAGAGCACGGACCAGTTACGGCTGTCCTATGCCAATCGGCTTTGCCTGTCGCATCTGCACCCGGTCTggcagtagc                           >  2:1862987/1‑93 (MQ=255)

                                                                      cccTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGa                        >  1:4135434/1‑100 (MQ=255)

                                                                        cTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATg                      <  1:2569310/100‑1 (MQ=255)

                                                                        cTTTTTCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATg                      <  1:304950/100‑1 (MQ=255)

                                                                         tttttCTTCCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATgg                     <  2:529639/100‑1 (MQ=255)

                                                                          ttttCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGa                    <  2:121359/100‑1 (MQ=255)

                                                                             tcttcCAGTTGGTCCAGAGTACGGTCCAGTGATGGCTGCTCGATGGCAATCGCTTTTGCCAGTGGAATTTGCGACTGGGCTGGAGGTAACTGATGGata                  <  2:3259572/99‑1 (MQ=255)

                                                                              cttcCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATAtt                >  2:627925/1‑100 (MQ=255)

                                                                              cttcCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATAtt                >  1:3138446/1‑100 (MQ=255)

                                                                               ttcCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATtg               <  1:1185189/100‑1 (MQ=255)

                                                                                tcCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATtgt              >  1:4125087/1‑100 (MQ=255)

                                                                                tcCAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGCCTGGAGGTAACTGATGGATATtgt              >  1:1239475/1‑100 (MQ=255)

                                                                                 ccAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATtgtg             >  1:3236067/1‑100 (MQ=255)

                                                                                  cAGTTGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTgtgt            <  1:2283336/100‑1 (MQ=255)

                                                                                   aGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTgta           >  1:2434765/1‑100 (MQ=255)

                                                                                    gTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTgtaa          >  1:3505417/1‑100 (MQ=255)

                                                                                    gTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTgtaa          <  2:3004242/100‑1 (MQ=255)

                                                                                     ttGGTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTgtaac         <  1:376952/100‑1 (MQ=255)

                                                                                       ggTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTgtaacgt       >  1:3233174/1‑100 (MQ=255)

                                                                                       ggTCCAGAGCACGGACCAGTGATGGCTGATCGATGCCAATCGGTTTTGCCAGTTGAATTTGCGACTGGTCTGGATGTAACTGCTGGGTATTGTgtaa          >  2:3816463/1‑97 (MQ=255)

                                                                                        gTCCAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTgtaacgta      >  1:1656661/1‑100 (MQ=255)

                                                                                          ccAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTgtaacgtaac    >  1:963652/1‑100 (MQ=255)

                                                                                          ccAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTgtaacgtaac    >  1:210511/1‑100 (MQ=255)

                                                                                          ccAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTgtaacgtaac    <  2:1360844/100‑1 (MQ=255)

                                                                                          ccAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTgtaacgtaac    >  1:4031831/1‑100 (MQ=255)

                                                                                          ccAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTgtaacgtaac    >  1:3913681/1‑100 (MQ=255)

                                                                                          ccAGAGCACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTgtaacgtaac    >  1:3365986/1‑100 (MQ=255)

                                                                                            agagTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTGTAACGTAAccc  >  2:2128715/1‑100 (MQ=255)

GCTCTGCCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTGTAACGTAACCC  >  NC_000913/1720533‑1720724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A