Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F204 I0 R1	247	174.3	8556966	94.2%	8060661	99.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,720,828	C→T	53.8%	intergenic (‑4/‑197)	slyA ← / → ydhI	global transcriptional regulator/DUF1656 family putative inner membrane efflux pump associated protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,720,828	0	C	T	53.8%	11.4 / 173.3	128	intergenic (‑4/‑197)	slyA/ydhI	global transcriptional regulator/DUF1656 family putative inner membrane efflux pump associated protein
Reads supporting (aligned to +/- strand): ref base C (34/25); new base T (40/29); total (74/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.05e-01

Only 100 of 131 total aligned reads displayed.

GGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTATTGCTGCATTTGGTTACCG  >  NC_000913/1720729‑1720927

ggTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTc                                                                                                     >  2:649190/1‑100 (MQ=255)

   ttGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTAtc                                                                                                  <  1:1318601/100‑1 (MQ=255)

   ttGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTAtc                                                                                                  <  2:1526019/100‑1 (MQ=255)

     gtgtTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCAtctc                                                                                                >  2:1725922/1‑100 (MQ=255)

      tgtTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTcc                                                                                               >  1:681071/1‑100 (MQ=255)

       gtTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCt                                                                                              >  1:3721785/1‑100 (MQ=255)

       gtTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCt                                                                                              >  1:966153/1‑100 (MQ=255)

        ttAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCtt                                                                                             <  2:2767324/100‑1 (MQ=255)

         tAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTCGTGGCGATTCCAATTTTATCTCCTta                                                                                            >  1:3984280/1‑100 (MQ=255)

         tAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTta                                                                                            <  1:1614780/100‑1 (MQ=255)

           aCTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTtata                                                                                          >  2:2845259/1‑100 (MQ=255)

            cTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATaa                                                                                         >  2:3754267/1‑100 (MQ=255)

            cTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATaa                                                                                         >  2:2908464/1‑100 (MQ=255)

             tCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAAt                                                                                        <  1:3066992/100‑1 (MQ=255)

              ccAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAAtt                                                                                       >  2:801735/1‑100 (MQ=255)

              ccAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATATCCTTATAAtt                                                                                       >  1:4132015/1‑100 (MQ=255)

               cAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTa                                                                                      >  1:886112/1‑100 (MQ=255)

               cAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTa                                                                                      >  1:1090706/1‑100 (MQ=255)

               cAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTa                                                                                      <  1:584345/100‑1 (MQ=255)

                aGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAg                                                                                     <  2:1199316/100‑1 (MQ=255)

                 gCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGc                                                                                    >  2:212022/1‑100 (MQ=255)

                  cGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCt                                                                                   >  1:264044/1‑100 (MQ=255)

                    gTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGTACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTg                                                                                 >  1:2993267/1‑100 (MQ=255)

                      ttCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCt                                                                               >  2:714576/1‑100 (MQ=255)

                      ctCAGGCGATGGTCGACCAGAGCACGCCAGATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCGTATAATTAGCTTg                                                                                 <  1:2065740/97‑1 (MQ=255)

                       tCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTa                                                                              >  2:1309773/1‑100 (MQ=255)

                         aGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAg                                                                            <  1:3120432/100‑1 (MQ=255)

                         aGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAg                                                                            >  2:3860860/1‑100 (MQ=255)

                          ggCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGa                                                                           <  1:3003640/100‑1 (MQ=255)

                            cGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGata                                                                         <  2:3917828/100‑1 (MQ=255)

                             gATGCTCTATCAGAGCACGCAATATGCGCACCAACCTTGCCAGATTTGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTTCTAAGa                                                                           <  1:1417018/97‑1 (MQ=255)

                               tGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGAtatta                                                                      <  2:1641212/100‑1 (MQ=255)

                               tGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGAtatta                                                                      <  1:2116906/100‑1 (MQ=255)

                                 gTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATg                                                                    >  1:1207705/1‑100 (MQ=255)

                                  tCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGc                                                                   <  2:1640745/100‑1 (MQ=255)

                                   cTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTCAGATATTATGCg                                                                  <  2:2810051/100‑1 (MQ=255)

                                   cTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCg                                                                  <  2:4015023/100‑1 (MQ=255)

                                   cTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCg                                                                  >  2:3060497/1‑100 (MQ=255)

                                    tATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCgg                                                                 >  2:462221/1‑100 (MQ=255)

                                       cAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCtt                                                              >  1:2615576/1‑100 (MQ=255)

                                       cAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAAGTTTATCTCCTTATAATGAGCTTGCTAAGATATTATGCGGCtt                                                              <  1:219840/100‑1 (MQ=255)

                                       cAGAGCACGCCATATGCGCACCAACCGTGCCAAATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCtt                                                              >  2:4059417/1‑100 (MQ=255)

                                         gagCACGCCATATGCGCACCAATCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCtttt                                                            <  1:1543694/100‑1 (MQ=255)

                                          agCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCtttt                                                            >  1:540412/1‑99 (MQ=255)

                                          agCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCtttt                                                            <  1:2094704/99‑1 (MQ=255)

                                          agCACGCCATATGCGCACCAACCGTGACAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTa                                                           >  2:2590410/1‑100 (MQ=255)

                                           gCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAg                                                          <  2:187054/100‑1 (MQ=255)

                                           gCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAg                                                          >  2:3355572/1‑100 (MQ=255)

                                              cGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAAt                                                       >  1:3039897/1‑100 (MQ=255)

                                              cGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAAt                                                       >  2:1389454/1‑100 (MQ=255)

                                              cGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAAt                                                       >  1:481830/1‑100 (MQ=255)

                                                ccATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAg                                                     >  2:1188877/1‑100 (MQ=255)

                                                 cATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAgt                                                    >  2:1382854/1‑100 (MQ=255)

                                                 cATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAgt                                                    >  2:1475940/1‑100 (MQ=255)

                                                   tatGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAgtgt                                                  <  2:3139661/100‑1 (MQ=255)

                                                   tatGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAg                                                     <  1:3964644/97‑1 (MQ=255)

                                                   tatGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAgtgt                                                  >  2:3406424/1‑100 (MQ=255)

                                                   tatGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAgtgt                                                  <  2:2939378/100‑1 (MQ=255)

                                                    atGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGtgtg                                                 >  2:2469059/1‑100 (MQ=255)

                                                     tGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTgc                                                >  1:3325955/1‑100 (MQ=255)

                                                        gcACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTgcagc                                             >  2:3777857/1‑100 (MQ=255)

                                                         cACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTgcagca                                            >  1:3389072/1‑100 (MQ=255)

                                                         cACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTgcagca                                            >  1:576081/1‑100 (MQ=255)

                                                           ccAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAAt                                          <  1:185499/100‑1 (MQ=255)

                                                           ccAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAAt                                          >  2:2337013/1‑100 (MQ=255)

                                                            cAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAAtt                                         >  2:3792117/1‑100 (MQ=255)

                                                             aaCCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTg                                        >  2:2257039/1‑100 (MQ=255)

                                                                 gTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTAtt                                    >  1:3747685/1‑100 (MQ=255)

                                                                  tGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTg                                   >  1:618366/1‑100 (MQ=255)

                                                                    ccAGATCAGGACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCt                                 >  1:1562874/1‑100 (MQ=255)

                                                                    ccAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCt                                 >  2:4107914/1‑100 (MQ=255)

                                                                    ccAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCt                                 <  2:2506218/100‑1 (MQ=255)

                                                                    ccAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCt                                 >  2:3289297/1‑100 (MQ=255)

                                                                    ccAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCt                                 >  1:840660/1‑100 (MQ=255)

                                                                    ccAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCt                                 <  1:979453/100‑1 (MQ=255)

                                                                    ccAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCt                                 >  1:1663692/1‑100 (MQ=255)

                                                                     cAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTa                                >  1:3117515/1‑100 (MQ=255)

                                                                     cAGATCAGAACCTAGTGGCGATTCCAAGTTTATCTCCTTATAATTATCTTACTAAGATATTATGCGGCTTTTATAATAGTGTGCATCAATTGTATTTCTa                                >  1:856218/1‑100 (MQ=255)

                                                                      aGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTaa                               <  1:796087/100‑1 (MQ=255)

                                                                       gATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTaaa                              <  1:137137/100‑1 (MQ=255)

                                                                         tCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAAc                            <  2:2960052/100‑1 (MQ=255)

                                                                          cAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACa                           >  1:2393555/1‑100 (MQ=255)

                                                                          cAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACa                           >  1:1804282/1‑100 (MQ=255)

                                                                           aGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACaa                          <  1:728727/100‑1 (MQ=255)

                                                                           aGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACaa                          <  2:3560826/100‑1 (MQ=255)

                                                                           aGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACaa                          >  2:3814783/1‑100 (MQ=255)

                                                                              aCCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATg                       <  1:1238047/100‑1 (MQ=255)

                                                                               ccTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGt                      >  2:1617598/1‑100 (MQ=255)

                                                                                  aGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTAtt                   >  2:142991/1‑100 (MQ=255)

                                                                                     ggCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTATtgct                >  2:2711302/1‑100 (MQ=255)

                                                                                       cGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTATtgctgc              >  1:3334256/1‑100 (MQ=255)

                                                                                         aTTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTATTGCTGCAt            <  2:2752772/100‑1 (MQ=255)

                                                                                          ttCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTATTGCTGCAtt           >  2:380899/1‑100 (MQ=255)

                                                                                             cAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTATTGCTGCATTTgg        <  1:1299500/100‑1 (MQ=255)

                                                                                             cAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTATTGCTGCATTTgg        >  2:1610597/1‑100 (MQ=255)

                                                                                              aaTTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTATTGCTGCATTTGGt       <  2:2004325/100‑1 (MQ=255)

                                                                                               aTTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTATTGCTGCATTTGGtt      >  1:960662/1‑100 (MQ=255)

                                                                                                 ttCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTATTGCTGCATTTGGTTAc    >  2:3211436/1‑100 (MQ=255)

                                                                                                 ttCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTATTGCTGCATTTGGTTAc    <  1:885318/100‑1 (MQ=255)

                                                                                                   cATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTATTGCTGCATTTGGTTACCg  >  2:2348550/1‑100 (MQ=255)

GGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTATTGCTGCATTTGGTTACCG  >  NC_000913/1720729‑1720927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A