Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F56 I0 R1	215	141.2	6785521	95.3%	6466601	99.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,307,386	A→T	65.0%	D410E (GAT→GAA)	yojI ←	microcin J25 efflux ABC transporter permease/ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,307,386	0	A	T	65.0%	114.9 / 123.3	118	D410E (GAT→GAA)	yojI	microcin J25 efflux ABC transporter permease/ATPase
Reads supporting (aligned to +/- strand): ref base A (28/13); new base T (40/36); total (69/49)
Fisher's exact test for biased strand distribution p-value = 1.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.70e-01

Only 100 of 119 total aligned reads displayed.

GTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCC  >  NC_000913/2307290‑2307484

gTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCa                                                                                                 <  2:160442/100‑1 (MQ=255)

gTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCa                                                                                                 <  1:1042660/100‑1 (MQ=255)

 ttGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCaa                                                                                                <  1:2323385/100‑1 (MQ=255)

  tGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCaaa                                                                                               <  2:768260/100‑1 (MQ=255)

   gCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAAc                                                                                              >  2:1066714/1‑100 (MQ=255)

    cTTAACTCAAGCTTATGAGCCCTTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTCGCGGGTTTGCCCTCCGGCCCCCGCAGGTGTTCAAAc                                                                                              >  1:889299/1‑99 (MQ=255)

    cTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACa                                                                                             >  1:3340916/1‑100 (MQ=255)

     ttAACTCAAGCTTATGAGCCATTTTCAGCTGCGCAAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAg                                                                                            >  1:2990018/1‑100 (MQ=255)

      tAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGc                                                                                           >  2:3356434/1‑100 (MQ=255)

      tAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGc                                                                                           >  2:1111063/1‑100 (MQ=255)

       aaCTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGcc                                                                                          <  2:327558/100‑1 (MQ=255)

         cTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAg                                                                                        <  1:359687/100‑1 (MQ=255)

         cTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAg                                                                                        >  1:2855091/1‑100 (MQ=255)

         cTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAg                                                                                        >  2:2350281/1‑100 (MQ=255)

         cTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCCGGTTAGCGGATTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAg                                                                                        <  2:877572/100‑1 (MQ=255)

          tCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGa                                                                                       <  2:1038994/100‑1 (MQ=255)

           cAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGAc                                                                                      >  2:858857/1‑100 (MQ=255)

            aaGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACTCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACa                                                                                     >  2:3262331/1‑100 (MQ=255)

             aGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACa                                                                                     >  1:1424330/1‑99 (MQ=255)

                 tATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTGCAAACAGCCCGACATCgg                                                                                 >  1:2758428/1‑99 (MQ=255)

                    gAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTaaa                                                                             >  1:1459412/1‑100 (MQ=255)

                     aGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAac                                                                            <  2:2616356/100‑1 (MQ=255)

                     aGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAac                                                                            <  2:2604384/100‑1 (MQ=255)

                     aGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAac                                                                            <  2:1907484/100‑1 (MQ=255)

                      gCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAaca                                                                           <  2:1453131/100‑1 (MQ=255)

                        cATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACt                                                                         >  2:1441334/1‑100 (MQ=255)

                            ttCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCg                                                                     >  2:373399/1‑100 (MQ=255)

                             tCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGc                                                                       >  1:982803/1‑97 (MQ=255)

                              cAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGaa                                                                   >  1:2203512/1‑100 (MQ=255)

                               aGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGaaa                                                                  >  1:2862373/1‑100 (MQ=255)

                                gCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGaaaa                                                                 >  1:290820/1‑100 (MQ=255)

                                gCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGaaaa                                                                 >  1:2844488/1‑100 (MQ=255)

                                gCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGaaaa                                                                 >  2:1521709/1‑100 (MQ=255)

                                gCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGaaaa                                                                 >  2:1523056/1‑100 (MQ=255)

                                gCTGCGCCAGCCACTTCTCAACCAGTTGAGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGGTCACCCAGCCAGACATCGGTCAACACTGCCGtaa                                                                  >  1:25299/1‑99 (MQ=255)

                                   gcgcCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACACCGGTAAACACTGCCGAAAACAg                                                              >  1:3238976/1‑100 (MQ=255)

                                     gccagccaCTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGtt                                                            >  2:93367/1‑100 (MQ=255)

                                     gccagccaCTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGtt                                                            >  2:2223965/1‑100 (MQ=255)

                                        agccaCTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGc                                                         >  2:2991232/1‑100 (MQ=255)

                                        agccaCTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGc                                                         <  1:2758132/100‑1 (MQ=255)

                                        agccaCTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGc                                                         <  2:59012/100‑1 (MQ=255)

                                        agccaCTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGc                                                         <  1:1756680/100‑1 (MQ=255)

                                        agccaCTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGc                                                         <  2:818290/100‑1 (MQ=255)

                                         gccaCTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCg                                                        <  2:522825/100‑1 (MQ=255)

                                          ttccgatctaaCCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGa                                                       <  2:1991394/91‑1 (MQ=255)

                                          ccaCTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGa                                                       <  1:3017640/100‑1 (MQ=255)

                                           caCTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGAt                                                      >  1:3250055/1‑100 (MQ=255)

                                           caCTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGAt                                                      >  1:1320184/1‑100 (MQ=255)

                                           caCTTCTCAACCAATTGCGGGTTAGCGGGTTCACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGAt                                                      >  2:2278730/1‑100 (MQ=255)

                                             cTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATaa                                                    <  1:1663517/100‑1 (MQ=255)

                                               tctcAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATc                                                  >  1:256441/1‑100 (MQ=255)

                                               tctcAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATc                                                  <  2:1226865/100‑1 (MQ=255)

                                               tctcAACCAGTTGCGGGTTAGCCGGTTTACCATCCGGGCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATc                                                  <  1:2281443/100‑1 (MQ=255)

                                                ctcAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCt                                                 >  1:525574/1‑100 (MQ=255)

                                                   aaCCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAGCAGTTTGCGATAATCTTcc                                              <  1:1603455/100‑1 (MQ=255)

                                                   aaCCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGaagatcggaa                                              >  1:1991381/1‑91 (MQ=255)

                                                    aCCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCg                                             >  2:477436/1‑100 (MQ=255)

                                                    aCCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCg                                             >  2:388221/1‑100 (MQ=255)

                                                     ccAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCgg                                            >  2:751662/1‑100 (MQ=255)

                                                        gTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGgttg                                         >  2:3174124/1‑100 (MQ=255)

                                                         ttGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGgttgt                                        <  2:950128/100‑1 (MQ=255)

                                                         ttGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGgttgt                                        <  2:1603778/100‑1 (MQ=255)

                                                            cGGGTTAGCGGGTTTCCCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTcg                                     <  2:2783484/100‑1 (MQ=255)

                                                             gggTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTcgc                                    >  1:3076473/1‑100 (MQ=255)

                                                             gggTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTcgc                                    <  1:83392/100‑1 (MQ=255)

                                                              ggTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTcgcc                                   <  1:1169144/100‑1 (MQ=255)

                                                              ggTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTcgcc                                   >  1:1998973/1‑100 (MQ=255)

                                                               gTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTcgccg                                  <  2:692566/100‑1 (MQ=255)

                                                               gTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTcgccg                                  <  1:84117/100‑1 (MQ=255)

                                                                  aGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTg                               >  1:2346446/1‑100 (MQ=255)

                                                                  aGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAACCTTCCGGTTGTTcgccgc                                 >  1:3211764/1‑98 (MQ=255)

                                                                  aGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTg                               >  2:3029243/1‑100 (MQ=255)

                                                                  aGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTg                               <  1:3305607/100‑1 (MQ=255)

                                                                   gCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGa                              >  2:888164/1‑100 (MQ=255)

                                                                   gCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGa                              >  2:2603202/1‑100 (MQ=255)

                                                                       gTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAgg                          <  2:290821/100‑1 (MQ=255)

                                                                           aCCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTg                      <  1:3014733/100‑1 (MQ=255)

                                                                           aCCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTg                      >  1:3316818/1‑100 (MQ=255)

                                                                            cccTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCGGGCTGTTCGCCGCTGACAGGTTTGc                     >  1:2021679/1‑100 (MQ=255)

                                                                            cccTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGc                     <  1:2910449/100‑1 (MQ=255)

                                                                            cccTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGc                     >  2:3293350/1‑100 (MQ=255)

                                                                             ccTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGcc                    >  1:1640982/1‑100 (MQ=255)

                                                                             ccTCCGGCCCCAGCAGTTGAACAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGcc                    <  1:1639137/100‑1 (MQ=255)

                                                                              cTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTCAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCa                   >  1:186337/1‑100 (MQ=255)

                                                                              cTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCa                   <  1:1109374/100‑1 (MQ=255)

                                                                                 cGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATcc                <  2:138466/100‑1 (MQ=255)

                                                                                  ggCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTTCCGAAAACAGTTTGCGATAATCTTCTGGTCGTTCGCCGCTAACAGGTTTTCCATc                 >  1:2306620/1‑98 (MQ=255)

                                                                                    ccccAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGc             >  1:498228/1‑100 (MQ=255)

                                                                                     nccAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCa            >  1:2418196/2‑100 (MQ=255)

                                                                                     cccAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCa            >  1:2673962/1‑100 (MQ=255)

                                                                                     cccAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCa            >  2:2329562/1‑100 (MQ=255)

                                                                                     cccAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCa            <  2:1640989/100‑1 (MQ=255)

                                                                                       cagcagTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCAGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAg          >  1:784973/1‑100 (MQ=255)

                                                                                        agcagTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGa         <  1:3039688/100‑1 (MQ=255)

                                                                                         gcagTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGAt        >  1:926543/1‑100 (MQ=255)

                                                                                         gcagTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGAt        <  1:1352537/100‑1 (MQ=255)

                                                                                          cagTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGAt        <  1:575877/99‑1 (MQ=255)

                                                                                           agTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGAttt      <  1:703781/100‑1 (MQ=255)

                                                                                            gttgttCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTc     <  1:881941/100‑1 (MQ=255)

                                                                                               gATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTcgcc  >  2:2270527/1‑100 (MQ=255)

GTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCC  >  NC_000913/2307290‑2307484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A