Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F56 I1 R1
|
165 |
53.8 |
2611523 |
96.4% |
2517508 |
99.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,307,386 |
A→T |
D410E (GAT→GAA) |
yojI ← |
microcin J25 efflux ABC transporter permease/ATPase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,307,386 | 0 | A | T | 94.0%
| 159.1
/ 0.8
| 50 | D410E (GAT→GAA) | yojI | microcin J25 efflux ABC transporter permease/ATPase |
| Reads supporting (aligned to +/- strand): ref base A (2/1); new base T (22/25); total (24/26) |
| Fisher's exact test for biased strand distribution p-value = 6.02e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01 |
CGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAA > NC_000913/2307289‑2307475
|
cGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTc < 1:951439/100‑1 (MQ=255)
cGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTc < 1:242752/100‑1 (MQ=255)
ttGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAGCCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCaa < 2:209782/100‑1 (MQ=255)
tGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCaaa < 1:646512/100‑1 (MQ=255)
tGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCaaa > 2:411306/1‑100 (MQ=255)
gCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAAc < 2:1020605/100‑1 (MQ=255)
cTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACa < 1:1058868/100‑1 (MQ=255)
ttAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAg > 2:655822/1‑100 (MQ=255)
aCTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCa > 1:1075797/1‑100 (MQ=255)
aCTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCa > 1:334526/1‑100 (MQ=255)
aCTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTGACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCa > 1:414071/1‑100 (MQ=255)
cAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGGTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGAc < 1:80498/100‑1 (MQ=255)
aaGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGAc > 1:84947/1‑99 (MQ=255)
gCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATc < 2:382639/100‑1 (MQ=255)
tATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTTACCTCCGACCCCAGCTGTTGTTTAAACAGCCAGACATc > 2:1022221/1‑97 (MQ=255)
tATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGt > 2:666431/1‑100 (MQ=255)
gAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTaa > 2:1178360/1‑99 (MQ=255)
cATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACt < 1:113385/100‑1 (MQ=255)
aTTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTg < 1:1204891/100‑1 (MQ=255)
aTTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTg < 1:1027004/100‑1 (MQ=255)
ttttCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGc < 1:560797/100‑1 (MQ=255)
tttCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGcc < 1:1125787/100‑1 (MQ=255)
ttCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCg > 2:1056744/1‑100 (MQ=255)
tCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGa > 2:413482/1‑100 (MQ=255)
aGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGaaa > 2:416625/1‑100 (MQ=255)
gCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGaaaa > 2:723388/1‑100 (MQ=255)
gccagccaCTTCTCAACCAGTTGCGGGTTAACGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCGGAAAACAGtt > 2:858112/1‑100 (MQ=255)
agccaCTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGc < 1:730131/100‑1 (MQ=255)
cAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTc > 1:163507/1‑100 (MQ=255)
aCCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTcc < 1:1022230/99‑1 (MQ=255)
cAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGgt < 1:986434/100‑1 (MQ=255)
cAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGgt < 2:946712/100‑1 (MQ=255)
ggTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTcgcc > 1:355384/1‑100 (MQ=255)
ggTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTcgcc > 1:24095/1‑100 (MQ=255)
aGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTg > 2:422054/1‑100 (MQ=255)
cGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGAc < 1:1173952/100‑1 (MQ=255)
cGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGAc > 2:233155/1‑100 (MQ=255)
ggTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAg < 1:1178371/100‑1 (MQ=255)
tttACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGt < 1:655826/100‑1 (MQ=255)
aCCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTg < 1:125248/100‑1 (MQ=255)
aCCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTg < 1:494817/100‑1 (MQ=255)
cccTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGc > 1:1196701/1‑100 (MQ=255)
cccTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGc > 1:47937/1‑100 (MQ=255)
cccTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGc > 2:546038/1‑100 (MQ=255)
ccTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGcc < 2:1075787/100‑1 (MQ=255)
tCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGCCAGGTTTGCCAt < 1:857584/100‑1 (MQ=255)
ccGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGCTTGTTCGCCGCTGACAGGTTTGCCATc > 2:678936/1‑100 (MQ=255)
ggCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCa > 1:176906/1‑100 (MQ=255)
gCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAg < 1:1071337/100‑1 (MQ=255)
cagcagTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCaa < 1:1210944/99‑1 (MQ=255)
|
CGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAA > NC_000913/2307289‑2307475
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
