Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A11 F212 I1 R1
|
174 |
80.0 |
3892211 |
96.7% |
3763768 |
99.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,307,386 |
A→T |
D410E (GAT→GAA) |
yojI ← |
microcin J25 efflux ABC transporter permease/ATPase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,307,386 | 0 | A | T | 93.5%
| 205.5
/ 3.5
| 62 | D410E (GAT→GAA) | yojI | microcin J25 efflux ABC transporter permease/ATPase |
| Reads supporting (aligned to +/- strand): ref base A (3/1); new base T (24/34); total (27/35) |
| Fisher's exact test for biased strand distribution p-value = 3.09e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 |
CGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCC > NC_000913/2307289‑2307484
|
cGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTc < 2:944099/100‑1 (MQ=255)
cGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTc < 2:1746459/100‑1 (MQ=255)
cGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTc < 2:1822197/100‑1 (MQ=255)
gTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCa < 1:559224/100‑1 (MQ=255)
tGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCaaa < 2:430782/100‑1 (MQ=255)
tGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCaa < 2:224836/99‑1 (MQ=255)
gCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAAc > 1:919736/1‑100 (MQ=255)
gCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAAc < 2:919737/100‑1 (MQ=255)
cattAACTAAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTTCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAAc > 1:1331260/3‑100 (MQ=255)
aaCTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGcc < 2:1666759/100‑1 (MQ=255)
cAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGAc > 2:1818595/1‑100 (MQ=255)
cAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGAc < 2:444649/100‑1 (MQ=255)
aaGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACa > 2:378943/1‑100 (MQ=255)
aGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACAt > 1:1296939/1‑100 (MQ=255)
ttATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCgg < 1:1875844/100‑1 (MQ=255)
aTGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTa > 2:1549122/1‑100 (MQ=255)
aTGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTa < 2:1825886/100‑1 (MQ=255)
tGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCCGCAGTTGTTCAAACAGCCAGACCTCGGTaa > 1:959020/1‑100 (MQ=255)
gAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTaaa > 1:393424/1‑100 (MQ=255)
aGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAac < 1:1144342/100‑1 (MQ=255)
ccATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAacac > 1:1252847/1‑100 (MQ=255)
ccATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAacac > 2:1299933/1‑100 (MQ=255)
ttCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCg < 2:1736216/100‑1 (MQ=255)
gCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGaaaa > 1:161991/1‑100 (MQ=255)
ccaCTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGa < 1:80789/100‑1 (MQ=255)
ccaCTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGa > 2:171816/1‑100 (MQ=255)
caCTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGAt < 2:1252851/100‑1 (MQ=255)
caCTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGa > 2:319876/1‑99 (MQ=255)
ttCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAAt < 2:1868410/100‑1 (MQ=255)
ctcAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCt > 2:880252/1‑100 (MQ=255)
ccAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCgg < 2:796601/100‑1 (MQ=255)
ccAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCgg < 2:161992/100‑1 (MQ=255)
cAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGgt < 2:87257/100‑1 (MQ=255)
gTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGgttg < 1:1306973/100‑1 (MQ=255)
gTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGgttg < 2:1805847/100‑1 (MQ=255)
gTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGgttg > 2:1023858/1‑100 (MQ=255)
tGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGgttgtt > 2:1667173/1‑100 (MQ=255)
gCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTc < 1:734013/100‑1 (MQ=255)
cGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTcg < 2:393426/100‑1 (MQ=255)
cGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTcg > 2:1382905/1‑100 (MQ=255)
cGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTcg < 2:1353106/100‑1 (MQ=255)
gggTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTcgc > 1:191674/1‑100 (MQ=255)
ggTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTcgcc < 1:162525/100‑1 (MQ=255)
ggTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTcgcc > 2:1698962/1‑100 (MQ=255)
ggTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTcgcc > 1:20803/1‑100 (MQ=255)
gTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTcgccg < 1:1932270/100‑1 (MQ=255)
tAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCt > 1:1651564/1‑100 (MQ=255)
aGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTg > 2:770243/1‑100 (MQ=255)
aGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTg > 2:1020765/1‑100 (MQ=255)
ggTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCTGGTTGTTCGCCGCTGACAg > 1:1739570/1‑100 (MQ=255)
aCCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTg < 1:289656/100‑1 (MQ=255)
cccTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGc < 2:128196/100‑1 (MQ=255)
cTCCGGCCCCAGCAGTTGTTCAAACAGTCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCa < 2:1401092/100‑1 (MQ=255)
tCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCAt > 2:1098142/1‑100 (MQ=255)
ccGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATc < 1:135268/100‑1 (MQ=255)
cccAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCa < 2:1094877/100‑1 (MQ=255)
ccAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCaa < 2:1381552/100‑1 (MQ=255)
cagTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGAtt < 2:1577659/100‑1 (MQ=255)
cagTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGAtt < 2:1578420/100‑1 (MQ=255)
agTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGAttt > 1:995688/1‑100 (MQ=255)
gttgttCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTc < 1:662972/100‑1 (MQ=255)
ttGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTcg < 1:43667/100‑1 (MQ=255)
gATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTcgcc > 2:409/1‑100 (MQ=255)
|
CGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCC > NC_000913/2307289‑2307484
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
