Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F56 I0 R1 215 141.2 6785521 95.3% 6466601 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,777,363 T→C 62.5% F83F (TTT→TTC)  yfjZ → CP4‑57 prophage; antitoxin of the YpjF‑YfjZ toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,777,3630TC62.5% 122.4 / 130.8 134F83F (TTT→TTC) yfjZCP4‑57 prophage; antitoxin of the YpjF‑YfjZ toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base T (27/23);  new base C (38/45);  total (66/68)
Fisher's exact test for biased strand distribution p-value = 3.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.16e-01

Only 100 of 136 total aligned reads displayed.

GTATTTCCACATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACGAGAGCAAGCATGAACACTC  >  NC_000913/2777265‑2777462
                                                                                                  |                                                                                                   
gTATTTCCACATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTTa                                                                                                    >  2:107368/1‑100 (MQ=255)
  aTTTCCACATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTTAcc                                                                                                  >  2:618016/1‑100 (MQ=255)
    ttCCACATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTg                                                                                                >  1:1283598/1‑100 (MQ=255)
     tCCACATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTTACCTGc                                                                                               >  1:2155530/1‑100 (MQ=255)
     tCCACATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTTACCTGc                                                                                               >  2:3304257/1‑100 (MQ=255)
     tCCACATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGc                                                                                               >  1:3024560/1‑100 (MQ=255)
      ccACATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCg                                                                                              >  1:474435/1‑100 (MQ=255)
      ccACATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCg                                                                                              <  1:2980484/100‑1 (MQ=255)
        acaTTTTATCAGCAAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCg                                                                                              <  1:1193527/98‑1 (MQ=255)
         caTTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTTACCTGCGAAg                                                                                           <  2:3180724/100‑1 (MQ=255)
          aTTTTATCAGCGAGATAGAGTCGATGCTGACCACTGGTGAACTGAAGCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGc                                                                                          <  2:463815/100‑1 (MQ=255)
          aTTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGc                                                                                          <  2:2563873/100‑1 (MQ=255)
           ttttATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACGACGGTTTTACCTGCGAAGcc                                                                                         <  1:2067279/100‑1 (MQ=255)
             ttATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCTAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGa                                                                                       >  2:2862070/1‑100 (MQ=255)
             ttATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGa                                                                                       >  1:2288299/1‑100 (MQ=255)
             ttATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGa                                                                                       <  2:1584737/100‑1 (MQ=255)
              tATCAGCCAGCTAGAGTCGATGCGGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGAt                                                                                      <  1:2221977/100‑1 (MQ=255)
                tCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATAc                                                                                    <  2:1342413/100‑1 (MQ=255)
                   gCCCGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCCGTCCCACAACGGTTTCACCTGCGAAGcc                                                                                         >  1:3244417/1‑92 (MQ=39)
                   gCCAGATATAGTCGATGCTGACCACTGGTGAACTGAATGCCCGCCATGCCCAATGCGTCACCCTGTGCCACAACGGTTTCACCTGCGAAGCCAATAct                                                                                   >  1:3225618/1‑98 (MQ=255)
                      aGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTgg                                                                              <  1:978394/100‑1 (MQ=255)
                      aGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTgg                                                                              >  2:583793/1‑100 (MQ=255)
                      aGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTgg                                                                              <  2:1645580/100‑1 (MQ=255)
                       gATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGt                                                                             <  2:1650486/100‑1 (MQ=255)
                       gATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGt                                                                             <  2:2143432/100‑1 (MQ=255)
                         tAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAg                                                                           >  1:2357696/1‑100 (MQ=255)
                          agagTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGt                                                                          <  1:14840/100‑1 (MQ=255)
                              tCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCg                                                                      <  1:1474966/100‑1 (MQ=255)
                               cGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGTgg                                                                     <  1:2461395/100‑1 (MQ=255)
                               cGATGCTGACCACTGGTGAACGGAATCTCCGCCATGCCCAATGCGTCACCCTGTAACACAAAGGTATTACCTGCGGAGCCGATACTCTTGGTAGTTGCgg                                                                     >  2:1272369/1‑100 (MQ=255)
                                gATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGc                                                                    <  2:2962442/100‑1 (MQ=255)
                                  tGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTa                                                                  <  2:1252054/100‑1 (MQ=255)
                                  tGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTa                                                                  >  1:1252050/1‑100 (MQ=255)
                                  tGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTa                                                                  <  1:2763066/100‑1 (MQ=255)
                                  tGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTa                                                                  >  2:1431458/1‑100 (MQ=255)
                                    ttGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACg                                                                <  2:1479642/99‑1 (MQ=255)
                                    cTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACg                                                                <  2:260442/100‑1 (MQ=255)
                                       aCCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTTACCGGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGtat                                                             <  2:967286/100‑1 (MQ=255)
                                        ccACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGtata                                                            <  1:2327336/100‑1 (MQ=255)
                                        ccACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGtata                                                            >  2:1544380/1‑100 (MQ=255)
                                          aCTGGTGAACTGAATCCCCGCCGTGCCAAATGCGTCACCCGGTCCCACGACGGTCAGACCTGCGTTGCCGATCCTCGTGGGAGTTGCGGCTACGTTTACa                                                          >  1:1629454/1‑100 (MQ=19)
                                          aCTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACa                                                          >  1:423183/1‑100 (MQ=255)
                                             ggTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTTACCTGCGCAGCCCCTACCCTCGGTAGTTGCGGCTACGCATACAt                                                         >  1:121965/1‑98 (MQ=21)
                                             ggTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCg                                                       >  2:284962/1‑100 (MQ=255)
                                               tGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCt                                                     >  2:2399441/1‑100 (MQ=255)
                                                  ncTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGtt                                                  >  2:3075254/2‑100 (MQ=255)
                                                   cTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGttt                                                 <  1:1021539/100‑1 (MQ=255)
                                                    tGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTa                                                >  2:2904642/1‑100 (MQ=255)
                                                    tGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTa                                                <  2:37286/100‑1 (MQ=255)
                                                    tGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTa                                                <  2:335693/100‑1 (MQ=255)
                                                     gAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTAc                                               >  2:2645289/1‑100 (MQ=255)
                                                     gAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTAc                                               >  2:2935605/1‑100 (MQ=255)
                                                      aaTCCCCGCCATGCCCAATGCGTCCCTCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTAcc                                              <  1:325709/100‑1 (MQ=255)
                                                      aaTCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTGTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGttt                                                 <  1:3383524/97‑1 (MQ=255)
                                                       aTCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTAccc                                             <  2:151000/100‑1 (MQ=255)
                                                       aTCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTAccc                                             >  1:2733120/1‑100 (MQ=255)
                                                        tCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTAcccc                                            <  1:1726153/100‑1 (MQ=255)
                                                        tCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTAcccc                                            <  2:1283602/100‑1 (MQ=255)
                                                        tCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTAcccc                                            <  1:2457666/100‑1 (MQ=255)
                                                         ccccGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCa                                           >  1:1578383/1‑100 (MQ=255)
                                                          cccGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCAc                                          <  2:3219778/100‑1 (MQ=255)
                                                               cATGCCCAATGCGTCACCCTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAAc                                     >  1:2618126/1‑100 (MQ=255)
                                                                aTGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACg                                    <  2:420669/100‑1 (MQ=255)
                                                                  gCCGAAGGCGTCCCTCGGTACCGCAAGGGGTTTGCCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGGTTAcccc                                            <  1:3153593/90‑1 (MQ=18)
                                                                   cccAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTGCCCCACTCAACGcta                                 >  1:2589731/1‑100 (MQ=255)
                                                                    ccAATGCGTCACCCTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGctaa                                >  2:908251/1‑100 (MQ=255)
                                                                     cAATGCGTCACCCTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGctaac                               <  2:3244832/100‑1 (MQ=255)
                                                                     cAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGctaac                               <  1:3083130/100‑1 (MQ=255)
                                                                     cAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGctaac                               <  2:2000430/100‑1 (MQ=255)
                                                                      aaTGCGTCACCCTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGctaact                              >  2:1640444/1‑100 (MQ=255)
                                                                        tGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGctaactaa                            <  2:946730/100‑1 (MQ=255)
                                                                          cGTCACCCTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAACTAAtt                          >  1:3203343/1‑100 (MQ=255)
                                                                          cGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAACTAAtt                          >  1:2986021/1‑100 (MQ=255)
                                                                           gTCACCCTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAACTAAttt                         >  2:1292607/1‑100 (MQ=255)
                                                                            tCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAGCGCTAACTAATTTc                        >  1:1373293/1‑100 (MQ=255)
                                                                            tCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTc                        <  2:1093633/100‑1 (MQ=255)
                                                                            tCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTc                        <  1:886960/100‑1 (MQ=255)
                                                                             cACCCTGTACCGCAGCGGTTTCTCCTGCGGAGCCGATTCTCTTGTTAGTTGCGGCTAGGTATACATCGCTGTTTTCCCCGCTCATCGCTAACTAAttcc                        <  1:607704/99‑3 (MQ=255)
                                                                             cACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCa                       >  2:830802/1‑100 (MQ=255)
                                                                             cACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCa                       >  2:2230157/1‑100 (MQ=255)
                                                                              aCCCTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCAc                      <  1:1642521/100‑1 (MQ=255)
                                                                              aCCCTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCAc                      <  1:2642231/100‑1 (MQ=255)
                                                                               cccTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTATTTACCCCACTCAACGCTAACTAATTTCACg                     >  1:1505883/1‑100 (MQ=255)
                                                                               cccTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACg                     <  2:977325/100‑1 (MQ=255)
                                                                                ccTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACga                    >  2:1036177/1‑100 (MQ=255)
                                                                                 cTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACgag                   <  1:861240/100‑1 (MQ=255)
                                                                                     aCCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACTCCACTCAACGCTAACTAATTTCACGAGagca               >  1:2372146/1‑100 (MQ=255)
                                                                                      ccACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACGAGagcaa              >  2:1723878/1‑100 (MQ=255)
                                                                                      ccACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACGAGagcaa              >  2:2393188/1‑100 (MQ=255)
                                                                                      ccACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACGAGagcaa              <  1:973739/100‑1 (MQ=255)
                                                                                      ccACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACGAGagcaa              >  1:236605/1‑100 (MQ=255)
                                                                                       cacaACGGTTTCACCTGCGAAGCCGCTACTCTTGGTAGTTGCGGCTACGTATACATCTCTGTTTACCCCACTCCACGCTAACTAATTTCACGAGagcaag             >  1:435686/1‑100 (MQ=25)
                                                                                       cacaACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACGAGagcaag             <  2:2321958/100‑1 (MQ=255)
                                                                                        acaACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGATGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACGAGagcaagc            >  1:1801771/1‑100 (MQ=255)
                                                                                        acaACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACGAGagcaagc            <  2:1002683/100‑1 (MQ=255)
                                                                                         caACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACGAGagcaagca           >  2:1901864/1‑100 (MQ=255)
                                                                                         caACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACGAGagcaagc            >  2:747012/1‑99 (MQ=255)
                                                                                              gTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACGAGAGCAAGCATGAac      >  1:230794/1‑100 (MQ=255)
                                                                                               tttcACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACGAGAGCAAGCATGAaca     >  1:2823578/1‑100 (MQ=255)
                                                                                                  tACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACGAGAGCAAGCATGAACActc  <  1:335512/100‑1 (MQ=255)
                                                                                                  |                                                                                                   
GTATTTCCACATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACGAGAGCAAGCATGAACACTC  >  NC_000913/2777265‑2777462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A