Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F256 I0 R1	328	102.7	5123138	93.4%	4785010	99.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,777,363	T→C	100%	F83F (TTT→TTC)	yfjZ →	CP4‑57 prophage; antitoxin of the YpjF‑YfjZ toxin‑antitoxin system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,777,363	0	T	C	98.0%	315.4 / ‑4.0	96	F83F (TTT→TTC)	yfjZ	CP4‑57 prophage; antitoxin of the YpjF‑YfjZ toxin‑antitoxin system
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (54/39); total (56/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

TATTTCCACATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACGAGAGCAAGCATGA  >  NC_000913/2777266‑2777456

tATTTCCACATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCAc                                                                                             <  2:175381/100‑1 (MQ=255)

 aTTTCCACATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGACCAATGCGTCACCCTGTACCACAACGGTTTCAcc                                                                                            >  1:1681678/1‑100 (MQ=255)

   ttCCACATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTg                                                                                          <  1:1896746/100‑1 (MQ=255)

     ccACATTTTATCAGCCAGATAGAGTCGATGCTGAGCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCg                                                                                        <  2:1352294/100‑1 (MQ=255)

     ccACATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCg                                                                                        >  1:2229405/1‑100 (MQ=255)

      cacaTTTTATCAGCCAGATAGAGTCGATGCTTACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGa                                                                                       <  2:2548230/100‑1 (MQ=255)

       acaTTTTGTCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGaa                                                                                      >  1:1348306/1‑100 (MQ=255)

        caTTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACGCTGTACCACAACGGTGTCACCTGCGa                                                                                       >  2:1100010/1‑98 (MQ=255)

          ccgatctaGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGcc                                                                                   <  1:167773/93‑1 (MQ=255)

           tttATCAGCCAGATAGAGTCGATGCTGACCACTGGTGATCTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCg                                                                                  <  2:1807732/100‑1 (MQ=255)

           tttATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCg                                                                                  >  1:916323/1‑100 (MQ=255)

              aTCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATa                                                                               >  2:329431/1‑100 (MQ=255)

                cAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATAct                                                                             >  1:1374369/1‑100 (MQ=255)

                 aGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGccagctcgg                                                                            >  2:167771/1‑93 (MQ=255)

                 aGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATActc                                                                            >  1:365466/1‑100 (MQ=255)

                 aGCCAGATAGAGTAGATGCTGACCACTGGTGAACTGAACCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATAct                                                                             >  2:1581781/1‑99 (MQ=255)

                  gCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATActct                                                                           >  2:1833766/1‑100 (MQ=255)

                   ccAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCtt                                                                          <  1:1066911/100‑1 (MQ=255)

                     aGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTgg                                                                        >  1:2440529/1‑100 (MQ=255)

                     aGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTgg                                                                        <  2:2515489/100‑1 (MQ=255)

                      gATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGt                                                                       <  2:1315262/100‑1 (MQ=255)

                      gATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGt                                                                       <  1:356024/100‑1 (MQ=255)

                      gATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGt                                                                       <  2:911547/100‑1 (MQ=255)

                       aTAGAGTCGATGCTGACCACTTGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTa                                                                      <  2:1305864/100‑1 (MQ=255)

                           agTGGATGCTGACGACTGGTGTACTGAATCTGCGCCATGCCCAATGCGTCATTTTGTTGCATAACGGTTTGACCTGCGACGCGGGTACTTTTGGTAGtt                                                                   >  2:1325377/1‑99 (MQ=255)

                             tCGATGGTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCg                                                                <  1:1026726/100‑1 (MQ=255)

                              cGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCgg                                                               >  1:2093404/1‑100 (MQ=255)

                               gATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGc                                                              <  2:976574/100‑1 (MQ=255)

                                aTGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGTAGCCGATACTCTTGGTAGTTGCGGCt                                                             <  1:1558057/100‑1 (MQ=255)

                                  gCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTAc                                                           >  2:133234/1‑100 (MQ=255)

                                   cTGACCACTGGTGCACTGAATCTACGACATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCAGCGAAGCCGATACTCCTGGTAGTCGCGGCTACg                                                          >  2:395654/1‑100 (MQ=21)

                                   cTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACg                                                          <  1:2215622/100‑1 (MQ=255)

                                   cTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACg                                                          >  2:1992822/1‑100 (MQ=255)

                                   cTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTCCGAAGCCGATACTCTTGGTAGTTGCGGCTACg                                                          >  2:857508/1‑100 (MQ=255)

                                    tGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGt                                                         >  2:195535/1‑100 (MQ=255)

                                    tGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGt                                                         >  2:418173/1‑100 (MQ=255)

                                       ccACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGtata                                                      <  1:1957047/100‑1 (MQ=255)

                                          cTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACAt                                                   >  1:1275840/1‑100 (MQ=255)

                                          cTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACAt                                                   >  1:914014/1‑100 (MQ=255)

                                           tGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATc                                                  >  1:2485754/1‑100 (MQ=255)

                                            ggTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCg                                                 >  2:196252/1‑100 (MQ=255)

                                             gTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGc                                                <  1:1695148/100‑1 (MQ=255)

                                              ttaaCTGAATCCCCGCCATGCCCACTGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCg                                                 <  2:2480386/96‑1 (MQ=255)

                                              tGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCt                                               >  1:500075/1‑100 (MQ=255)

                                              tGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTATCACCTGCGAAGCCGATCCTCTTGGTAGTTGCGTCTACGTATACATCGc                                                >  2:2201121/1‑99 (MQ=21)

                                              tGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTATACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCt                                               >  1:1801379/1‑100 (MQ=255)

                                               gAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTg                                              >  1:2512007/1‑100 (MQ=255)

                                                aaCTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTg                                              <  1:624038/99‑1 (MQ=255)

                                                aaCTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGt                                             >  2:1814143/1‑100 (MQ=255)

                                                 aCTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATCCTCTTGGTAGTTGCGGCTACGTATACATCGCTGtt                                            >  1:51045/1‑100 (MQ=255)

                                                 aCTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGtt                                            >  1:922968/1‑100 (MQ=255)

                                                 aCTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGtt                                            >  1:1256005/1‑100 (MQ=255)

                                                 aCTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGtt                                            >  2:14442/1‑100 (MQ=255)

                                                  ccgAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGttt                                           <  1:2494794/98‑1 (MQ=255)

                                                   tGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTa                                          >  2:795177/1‑100 (MQ=255)

                                                    gAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTAc                                         >  2:914751/1‑100 (MQ=255)

                                                      aTCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTAccc                                       >  1:1224777/1‑100 (MQ=255)

                                                       tCCCCGCCATGCCCAATGCGTCACCCTGTACCACCACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTAcccc                                      <  2:886987/100‑1 (MQ=255)

                                                       tCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTAcccc                                      >  1:1151933/1‑100 (MQ=255)

                                                        ccccGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCa                                     >  2:872053/1‑100 (MQ=255)

                                                        ccccGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTGGGTAGATGCGGCTATGTATACATCGCTGTTTACCCCa                                     >  2:1729072/1‑100 (MQ=21)

                                                         cccGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTAcccccc                                    >  2:52561/1‑98 (MQ=255)

                                                         cccGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCAc                                    >  1:1298733/1‑100 (MQ=255)

                                                          ccGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACt                                   <  1:1020781/100‑1 (MQ=255)

                                                          ccGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACt                                   <  1:1301140/100‑1 (MQ=255)

                                                          ccGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACt                                   >  1:2503235/1‑100 (MQ=255)

                                                            gCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCa                                 <  1:2115421/100‑1 (MQ=255)

                                                            gCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCa                                 >  2:1074639/1‑100 (MQ=255)

                                                              cATGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAAc                               <  1:2505398/100‑1 (MQ=255)

                                                                tGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGc                             <  1:133236/100‑1 (MQ=255)

                                                                tGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGc                             <  2:974789/100‑1 (MQ=255)

                                                                tGCCCAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGc                             <  1:364827/100‑1 (MQ=255)

                                                                  cccAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGcta                           <  2:2184681/100‑1 (MQ=255)

                                                                   ccAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGctaa                          <  1:2023020/100‑1 (MQ=255)

                                                                   ccAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGctaa                          >  2:1867327/1‑100 (MQ=255)

                                                                    cAATGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGctaac                         <  2:54946/100‑1 (MQ=255)

                                                                     aaTGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGctaact                        >  1:259304/1‑100 (MQ=255)

                                                                           tCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTc                  <  1:2074755/100‑1 (MQ=255)

                                                                              cccTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCAc                >  2:1149932/1‑99 (MQ=255)

                                                                              cccTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACg               >  2:2291241/1‑100 (MQ=255)

                                                                              cccTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACg               >  1:395061/1‑100 (MQ=255)

                                                                              cccTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACg               <  2:1224769/100‑1 (MQ=255)

                                                                                cTGTACCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGAGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACgag             >  1:1724031/1‑100 (MQ=255)

                                                                                    aCCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACGAGagca         <  1:105239/100‑1 (MQ=255)

                                                                                    aCCACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACGAGagca         >  2:2482969/1‑100 (MQ=255)

                                                                                     ccACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACGAGagcaa        >  1:1099318/1‑100 (MQ=255)

                                                                                     ccACAACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACGAGagcaa        >  1:1031283/1‑100 (MQ=255)

                                                                                      cacaACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACGAGagcaag       >  1:307910/1‑100 (MQ=255)

                                                                                      cacaACGGGTTCACCTGCGACGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACGAGagcaag       <  2:597909/100‑1 (MQ=255)

                                                                                       acaACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACGAGagcaagc      >  1:1347576/1‑100 (MQ=255)

                                                                                       acaACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACGAGagcaagc      <  1:1239163/100‑1 (MQ=255)

                                                                                       acaACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACGAGagcaagc      >  1:2158479/1‑100 (MQ=255)

                                                                                        caACGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACGAGagcaagca     <  2:1251987/100‑1 (MQ=255)

                                                                                          aCGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTGCCCCACTCAACGCTAACTAATTGCACGAGagcaagca     <  2:74382/98‑1 (MQ=255)

                                                                                          aCGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACGAGAGCAAGCATg   <  1:2197074/100‑1 (MQ=255)

                                                                                           cGGTTTCACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACGAGAGCAAGCATGa  >  1:1535104/1‑100 (MQ=255)

TATTTCCACATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACGAGAGCAAGCATGA  >  NC_000913/2777266‑2777456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A