Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A11 F56 I1 R1
|
155 |
65.2 |
3164312 |
95.9% |
3034575 |
99.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,887,352 |
T→A |
intergenic (‑133/+226) |
ygcB ← / ← cysH |
Cascade complex anti‑viral R‑loop helicase‑annealase Cas3/phosphoadenosine phosphosulfate reductase; PAPS reductase, thioredoxin dependent |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,887,352 | 0 | T | A | 95.7%
| 228.7
/ 0.4
| 70 | intergenic (‑133/+226) | ygcB/cysH | Cascade complex anti‑viral R‑loop helicase‑annealase Cas3/phosphoadenosine phosphosulfate reductase; PAPS reductase, thioredoxin dependent |
| Reads supporting (aligned to +/- strand): ref base T (3/0); new base A (35/32); total (38/32) |
| Fisher's exact test for biased strand distribution p-value = 2.45e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01 |
CAGCGGGCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGACAGCCTC > NC_000913/2887258‑2887447
|
cAGCGGGCTATTTTTTTTTTTTTTTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTg > 2:1410753/1‑100 (MQ=255)
cAGCGGGCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTg < 1:590963/100‑1 (MQ=255)
cAGCGGGCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTg > 1:460856/1‑100 (MQ=255)
cAGCGGGCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTg < 2:818252/100‑1 (MQ=255)
cAGCGGGCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTg < 2:346776/100‑1 (MQ=255)
cAGCGGGCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTg > 1:213572/1‑100 (MQ=255)
cAGCGGGCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTg < 1:1550592/100‑1 (MQ=255)
aGCGGGCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTgg < 1:827155/100‑1 (MQ=255)
cGGGCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGt > 1:1035279/1‑100 (MQ=255)
gCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCg < 2:594302/100‑1 (MQ=255)
ttCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACTTTCGAATAGTTTGGGTTCGAACGCTg < 1:688205/100‑1 (MQ=255)
ttCGATGCTGCTCAGAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTg < 2:1075763/100‑1 (MQ=255)
gATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGcc > 1:1487581/1‑100 (MQ=255)
gATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGcc > 2:244513/1‑100 (MQ=255)
gATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGcc > 1:912460/1‑100 (MQ=255)
gATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGcc < 1:340481/100‑1 (MQ=255)
gATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGc > 1:1276655/1‑99 (MQ=255)
ctgctCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAg < 1:1562047/100‑1 (MQ=255)
ctCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGtt > 2:1115122/1‑100 (MQ=255)
tCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTg > 2:1129234/1‑100 (MQ=255)
cATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGa > 2:1255128/1‑100 (MQ=255)
aTAACTATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGa > 2:1145578/1‑99 (MQ=255)
aTAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGAt < 2:237751/100‑1 (MQ=255)
aaaTATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGAtcg > 2:962007/1‑98 (MQ=255)
atCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAat > 2:807350/1‑100 (MQ=255)
gAATTACAGTGATATACACATGTCTTGCTTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTTGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATc > 2:232485/1‑100 (MQ=255)
tACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCt > 2:870961/1‑100 (MQ=255)
aCAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTg < 1:1171477/100‑1 (MQ=255)
cAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTgg < 1:226454/100‑1 (MQ=255)
aGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTggg < 2:1487572/100‑1 (MQ=255)
aGTGATATAAACATGTCTTGCCTCACTGCAAAGAAGATACTAACATGCGAATAGTATAGGTTCGAGCGCTGGCCTCAGGTTGATAGAAATATCGCCTggg < 2:110638/100‑1 (MQ=255)
gTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTgggg > 1:1373324/1‑100 (MQ=255)
tGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGc < 1:69742/100‑1 (MQ=255)
tGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGc > 2:511192/1‑100 (MQ=255)
gATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCt > 2:1433327/1‑100 (MQ=255)
tataCACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCttt < 1:1427624/100‑1 (MQ=255)
tataCACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCttt < 1:1958/100‑1 (MQ=255)
taCACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTAGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTg > 1:1108846/1‑100 (MQ=255)
taCACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACACTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTg < 2:851903/100‑1 (MQ=255)
acacATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGt < 1:34212/100‑1 (MQ=255)
acaTGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTcc > 1:990009/1‑100 (MQ=255)
caTGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCa < 1:1452654/100‑1 (MQ=255)
cttCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATc < 2:757489/98‑1 (MQ=255)
gTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCt < 1:694491/100‑1 (MQ=255)
gTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCt < 2:1373317/100‑1 (MQ=255)
tCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATcag > 1:757491/1‑98 (MQ=255)
tCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTg < 1:1551218/100‑1 (MQ=255)
cTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTgg > 1:753271/1‑100 (MQ=255)
ncTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTACGAACGCTGGCCTCAGGTTAATAGCAATATCGACTGGGGCTTTTGTCAATCTGGa > 2:862355/2‑97 (MQ=255)
cTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAAcc < 2:1043157/99‑1 (MQ=255)
cTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTcgcg > 2:164076/1‑100 (MQ=255)
cTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTcgcg > 1:766257/1‑100 (MQ=255)
cTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTcgcg > 1:630210/1‑100 (MQ=255)
cTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTcgc > 2:800425/1‑99 (MQ=255)
cTGCAAAGAAGAGACTAACATACGAATAAGTTGGGTTCGAACGCTAGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTcacg > 2:151740/1‑100 (MQ=255)
cAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCATCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACATCGCGAAt > 2:639221/1‑100 (MQ=255)
aagaagAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGc < 1:557374/100‑1 (MQ=255)
agaagaGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCt < 1:1203383/100‑1 (MQ=255)
gaagaGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGTAACCTCGCGAATGc > 2:880175/1‑98 (MQ=255)
agagACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTaa > 2:273633/1‑100 (MQ=255)
gagaCTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAAc < 1:244513/100‑1 (MQ=255)
gaCTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGc > 1:1279627/1‑100 (MQ=255)
gaCTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGc > 1:440640/1‑100 (MQ=255)
aCTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGcc < 2:1222742/100‑1 (MQ=255)
cTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCa > 1:585583/1‑100 (MQ=255)
aaCATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGa > 2:115976/1‑100 (MQ=255)
cATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGACa < 1:1410761/100‑1 (MQ=255)
cATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGACa > 2:985705/1‑100 (MQ=255)
aTTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGGATGCTTAACGCCAGAc > 2:1338117/1‑98 (MQ=255)
gAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGACAGCCTc < 2:1561405/100‑1 (MQ=255)
|
CAGCGGGCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGACAGCCTC > NC_000913/2887258‑2887447
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
