Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F133 I1 R1
|
102 |
83.9 |
4092546 |
96.1% |
3932936 |
99.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,887,352 |
T→A |
intergenic (‑133/+226) |
ygcB ← / ← cysH |
Cascade complex anti‑viral R‑loop helicase‑annealase Cas3/phosphoadenosine phosphosulfate reductase; PAPS reductase, thioredoxin dependent |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,887,352 | 0 | T | A | 98.8%
| 298.9
/ ‑3.9
| 84 | intergenic (‑133/+226) | ygcB/cysH | Cascade complex anti‑viral R‑loop helicase‑annealase Cas3/phosphoadenosine phosphosulfate reductase; PAPS reductase, thioredoxin dependent |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base A (49/34); total (49/35) |
| Fisher's exact test for biased strand distribution p-value = 4.17e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01 |
TCAGCGGGCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGACAGCCTC > NC_000913/2887257‑2887447
|
tCAGCGGGCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGttt > 2:1668436/1‑100 (MQ=255)
tCAGCGGGCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGttt > 1:1575696/1‑100 (MQ=255)
aGCGGGCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTgg > 2:1182980/1‑100 (MQ=255)
gCGGGCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTggg > 2:752732/1‑100 (MQ=255)
ggCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTc > 1:1212238/1‑100 (MQ=255)
gCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCg < 1:752735/100‑1 (MQ=255)
tATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGaa > 2:768866/1‑100 (MQ=255)
tATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGaa > 1:1624538/1‑100 (MQ=255)
tttttCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACg > 1:99816/1‑100 (MQ=255)
ttttCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGc > 2:600794/1‑100 (MQ=255)
tttCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCt > 2:381011/1‑100 (MQ=255)
ttCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTg < 1:986657/100‑1 (MQ=255)
tCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTgg > 1:17300/1‑100 (MQ=255)
cGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTACCATTCGAATAGTTTGGGTTCGAACGCTGGc > 2:1817162/1‑100 (MQ=255)
cGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGc > 2:62343/1‑100 (MQ=255)
tgctgcTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTc < 1:1139218/100‑1 (MQ=255)
gctgctCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCa > 2:1933645/1‑100 (MQ=255)
gctgctCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCa > 1:1506549/1‑100 (MQ=255)
tgctCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAgg < 1:875001/100‑1 (MQ=255)
tgctCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAgg < 2:1367478/100‑1 (MQ=255)
tgctCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAgg > 2:833721/1‑100 (MQ=255)
gctCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGt > 2:435573/1‑100 (MQ=255)
cATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGa < 2:17300/100‑1 (MQ=255)
aTAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGAt < 2:1914162/100‑1 (MQ=255)
aTAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGAt < 2:955068/100‑1 (MQ=255)
aTAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGAt > 1:618425/1‑100 (MQ=255)
tAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATa > 1:1437762/1‑100 (MQ=255)
aaaTATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTTGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAg < 2:1262614/100‑1 (MQ=255)
tatCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGaaa < 1:1855231/100‑1 (MQ=255)
tCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAata < 1:768869/100‑1 (MQ=255)
gAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATc < 1:62343/100‑1 (MQ=255)
gAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATc > 2:371455/1‑100 (MQ=255)
aaTTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCg < 1:1668449/100‑1 (MQ=255)
aaTTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCg < 1:1815418/100‑1 (MQ=255)
aaTTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCg < 1:133345/100‑1 (MQ=255)
aTTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGc > 2:856205/1‑100 (MQ=255)
tACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCt < 1:1533657/100‑1 (MQ=255)
tACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCt > 2:1675092/1‑100 (MQ=255)
aCAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCTAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTg < 2:758486/100‑1 (MQ=255)
cAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTgg > 1:1330529/1‑100 (MQ=255)
gTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTgggg > 2:275738/1‑100 (MQ=255)
gATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCt > 1:1841362/1‑100 (MQ=255)
gATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCt > 2:1147171/1‑100 (MQ=255)
taCACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTg < 2:41092/100‑1 (MQ=255)
taCACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTg > 1:1814092/1‑100 (MQ=255)
cacaTGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTc < 2:540226/100‑1 (MQ=255)
cacaTGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTc > 1:543894/1‑100 (MQ=255)
cacaTGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTc > 2:1591082/1‑100 (MQ=255)
cacaTGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTc > 1:772489/1‑100 (MQ=255)
caTGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTcta > 2:1242307/1‑98 (MQ=255)
caTGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCa > 2:102601/1‑100 (MQ=255)
caTGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCa < 2:1112434/100‑1 (MQ=255)
gTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCt > 2:459354/1‑100 (MQ=255)
gTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCt < 2:772486/100‑1 (MQ=255)
gTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCt < 1:381012/100‑1 (MQ=255)
tCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTg < 1:1675105/100‑1 (MQ=255)
cTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCTAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCt > 1:1633735/1‑98 (MQ=255)
cTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTgg < 1:1821184/100‑1 (MQ=255)
tGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGaa > 1:726224/1‑100 (MQ=255)
tGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGaa > 1:1901628/1‑100 (MQ=255)
ccTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAAcc > 1:488338/1‑100 (MQ=255)
cTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCt > 1:339214/1‑100 (MQ=255)
cccTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTcg > 2:1041708/1‑100 (MQ=255)
cccTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTcg > 1:1301920/1‑100 (MQ=255)
ccTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTcgc > 2:1890123/1‑100 (MQ=255)
cTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTcgcg < 1:275739/100‑1 (MQ=255)
cTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTcgcg < 2:1624525/100‑1 (MQ=255)
cTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTcgcg < 2:488337/100‑1 (MQ=255)
cTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTcgcg < 2:1212232/100‑1 (MQ=255)
agaagaGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCt < 1:600796/100‑1 (MQ=255)
agaagaGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCt < 2:1437753/100‑1 (MQ=255)
agaagaGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCt < 1:371456/100‑1 (MQ=255)
aagaGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTa < 1:193942/100‑1 (MQ=255)
cTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCa > 1:838615/1‑100 (MQ=255)
aCATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGAc < 1:102602/100‑1 (MQ=255)
cATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGAc > 2:1841772/1‑99 (MQ=255)
cATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGACa > 2:587146/1‑100 (MQ=255)
cATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGACa > 2:1698462/1‑100 (MQ=255)
cATTCGAATAGTTTGGGTTCGAACGCTGCCCTCAGGTTGATCGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGACTGCTTAACGCCAGAc > 2:1002834/1‑99 (MQ=255)
tCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGACAGcc < 1:834838/100‑1 (MQ=255)
tCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGACAGcc < 2:813083/100‑1 (MQ=255)
cGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGACAGcca > 1:813086/1‑99 (MQ=255)
gAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGACAGCCTc > 2:581607/1‑100 (MQ=255)
gAATAGTTTGGGTTCGAACGCTGGCCTCAGATTCATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCAAGACAGCCTc > 2:1342985/1‑100 (MQ=255)
|
TCAGCGGGCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGACAGCCTC > NC_000913/2887257‑2887447
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
