Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F142 I1 R1	99	64.8	3148104	96.3%	3031624	99.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,278,298	C→T	intergenic (‑61/+272)	yejG ← / ← bcr	uncharacterized protein/bicyclomycin/cysteine/sulfonamide efflux transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,278,298	0	C	T	98.3%	202.5 / ‑4.2	60	intergenic (‑61/+272)	yejG/bcr	uncharacterized protein/bicyclomycin/cysteine/sulfonamide efflux transporter
Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (36/23); total (36/24)
Fisher's exact test for biased strand distribution p-value = 4.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

GCAGTCGATGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTT  >  NC_000913/2278201‑2278394

gCAGTCGATGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATtaa                                                                                                <  1:572427/100‑1 (MQ=255)

 cAGTCGATGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATtaac                                                                                               <  2:471561/100‑1 (MQ=255)

  aGTCGCTGGCCGATTGACAGTCGTAATTCACTCACTTATTTTCCCCCGATGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATtaaca                                                                                              <  2:1478833/100‑1 (MQ=255)

     cGATGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATc                                                                                           >  2:796839/1‑100 (MQ=255)

         ggACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGt                                                                                        >  2:1380344/1‑99 (MQ=255)

           aCGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACCATCCGTCtt                                                                                     <  2:70697/100‑1 (MQ=255)

           aCGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCtt                                                                                     <  1:37699/100‑1 (MQ=255)

           aCGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCtt                                                                                     <  2:1087161/100‑1 (MQ=255)

           aCGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCtt                                                                                     <  2:499474/100‑1 (MQ=255)

             gATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTta                                                                                   <  2:589020/100‑1 (MQ=255)

             gATTGAGAGTGGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACACCATTTTTAACACATCATTAACAATCCGTCTTta                                                                                   <  2:1030714/100‑1 (MQ=255)

               ttGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTtata                                                                                 >  1:971809/1‑100 (MQ=255)

                tGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATAc                                                                                <  1:249368/100‑1 (MQ=255)

                tGAGAATTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTtat                                                                                  <  2:548143/98‑1 (MQ=255)

                 gagaGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACa                                                                               <  2:977968/100‑1 (MQ=255)

                      ttGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCg                                                                          <  2:703544/100‑1 (MQ=255)

                         tAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTaa                                                                       <  1:1485800/100‑1 (MQ=255)

                          aaTGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTaaa                                                                      >  1:548906/1‑100 (MQ=255)

                           aTGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAg                                                                     >  1:443668/1‑100 (MQ=255)

                           aTGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAg                                                                     >  1:1050337/1‑100 (MQ=255)

                              aaGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGAtt                                                                  >  1:147075/1‑100 (MQ=255)

                                 tCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGAt                                                               <  2:116407/100‑1 (MQ=255)

                                  cACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATg                                                              >  1:1451437/1‑100 (MQ=255)

                                  cACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATg                                                              >  1:484417/1‑100 (MQ=255)

                                  cACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATg                                                              >  1:321399/1‑100 (MQ=255)

                                  cACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATg                                                              <  1:302044/100‑1 (MQ=255)

                                  cACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATg                                                              >  1:1024010/1‑100 (MQ=255)

                                      tATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTc                                                          >  2:683827/1‑100 (MQ=255)

                                       aTTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCg                                                         <  1:917751/100‑1 (MQ=255)

                                           tCCCCGGGAGCACATTCACTTCCCAATTTGGTTTCCTCAATTTTAACCCAATATTAACAATTCGTCTTTTTACAGATTGTAAAGATTGATGCCttcgc                                                       >  2:92358/1‑94 (MQ=255)

                                           tCCCCGGAAGTACATTCACTTCACAATTTGTTTACATCCATTTTAACACATAATTAACAATCCGTATTTATACAGATCGTAAAGATTGATGACTCGCCt                                                      >  2:114251/1‑99 (MQ=255)

                                                ggAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTgg                                                >  1:702367/1‑100 (MQ=255)

                                                   aGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCct                                             >  1:880127/1‑100 (MQ=255)

                                                   aGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCct                                             >  2:155244/1‑100 (MQ=255)

                                                     cacaTTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCctct                                           >  1:692187/1‑100 (MQ=255)

                                                     cacaTTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCctct                                           >  2:1183992/1‑100 (MQ=255)

                                                      acaTTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGACTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTa                                          >  1:15425/1‑100 (MQ=255)

                                                        aTTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATg                                        >  2:1351380/1‑100 (MQ=255)

                                                        aTTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATg                                        >  1:525599/1‑100 (MQ=255)

                                                          tcacttcacAATTTGTTTACATCAATTGTAACACATCATTAACAATCCGGCTTGATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGc                                      <  1:1556423/100‑1 (MQ=255)

                                                           cacttcacAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGct                                     >  1:353184/1‑100 (MQ=255)

                                                            acttcacAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGctc                                    >  1:164279/1‑100 (MQ=255)

                                                             cttcacAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGctct                                   >  2:484492/1‑100 (MQ=255)

                                                              ttcacAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTg                                  <  2:164279/100‑1 (MQ=255)

                                                                cacaATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGAt                                >  1:1115160/1‑100 (MQ=255)

                                                                cacaATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGAt                                >  1:326285/1‑100 (MQ=255)

                                                                cacaATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGAt                                >  2:1283632/1‑100 (MQ=255)

                                                                cacaATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGAt                                <  1:992073/100‑1 (MQ=255)

                                                                 acaATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGAtt                               <  1:683830/100‑1 (MQ=255)

                                                                   aaTTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTa                             <  1:408195/100‑1 (MQ=255)

                                                                     tttGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAg                           <  2:1247832/100‑1 (MQ=255)

                                                                     tttGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAg                           <  2:1070949/100‑1 (MQ=255)

                                                                          ttACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTACGTGGCCTCTATGGCTCTGATTTAAGTAata                      <  1:1057703/100‑1 (MQ=255)

                                                                             cATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCt                   >  1:1333566/1‑100 (MQ=255)

                                                                              aTCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATc                    >  1:1538705/1‑98 (MQ=255)

                                                                              aTCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTg                  >  2:94827/1‑100 (MQ=255)

                                                                              aTCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTg                  >  1:1192922/1‑100 (MQ=255)

                                                                               tCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGc                 >  1:1566291/1‑100 (MQ=255)

                                                                                cAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCg                >  2:1274081/1‑100 (MQ=255)

                                                                                cAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTAATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTatt                       >  2:1073556/1‑91 (MQ=255)

                                                                                  aTTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCggg              <  2:559912/100‑1 (MQ=255)

                                                                                      tAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTg          >  1:1555017/1‑100 (MQ=255)

                                                                                       aaCACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTgg         <  1:349800/100‑1 (MQ=255)

                                                                                            atcattAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACg    <  2:147075/100‑1 (MQ=255)

                                                                                              cattaACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGtt  >  2:1165158/1‑100 (MQ=255)

                                                                                              cattaACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGtt  >  2:1094085/1‑100 (MQ=255)

                                                                                              cattaACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGtt  >  1:916126/1‑100 (MQ=255)

GCAGTCGATGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTT  >  NC_000913/2278201‑2278394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A